A number of studies have measured collagen fibers and collagen
deposition in wound healing process with advances imaging techniques. However, these are performed by complicated methods and need specific tools. In search of the easier ways in routine histopathological laboratory, collagen measurement and staining pattern of wound healing process were observed in wounded skin of Sprague Dawley’s rat by using two different stains which are standard haematoxylin and eosin (H&E) and modified Masson’s
trichrome staining (MT). The comparison between these staining in wounded tissues was made to evaluate the advantages and disadvantages of both staining in wound healing study for 21 days postwounding. Tissues which stained with MT staining was then evaluated its collagen re-organization and density by using polarized light microscope with the aid of image analyzer software. Results showed that tissues stained with standard H&E could not be used to measure and differentiate the collagen deposition which is contradictory to MT staining. Wounded tissue stained with MT staining has showed a clear view of collagen fibers deposition
and re-organisation compared to H&E staining. This finding could validate the using of modified MT staining which leads to accurate histopathological analysis and observation in wound healing study.

Corona Discharge Therapy,an evidence-based therapeutic modality, was used on a 40-year-old female Malaysian elephant. The elephant was on systemic therapy for her wounds for 5 days. All therapies were then stopped for 4 days. Corona Discharge Therapy was subsequently applied on both the hind limb joints for 3 sessions. The non-invasive
treatment was made with a multi-patented, electronic therapeutic device called VET Sonotron without the concurrent use of drugs. Corona discharge beam (CDB) of the device was used to relieve the joint pain and inflammation. The skin surface of the elephant joints was scanned with the CDB emanating from a discharge electrode to
which was applied periodic bursts of radio frequency at 0.43 mHz, pulsed at 1 kHz of sound frequency. This therapy showed remarkable anti-inflammatory effects.