The treatment of skin wounds with tissue-loss is one of the most important issues in the field of veterinary medicine. It is aimed to improve the wound faster and without contamination by doing various studies in this subject. In this study, laser phototherapy was preferred considering the same target.      This study was carried out on 20 cats and 20 dogs of different ages, races, and genders, who were brought to Surgery clinic of Animal Hospital at Kirikkale University. These patients were divided into 4 groups, two of which were study and two of which were a control group. In the study group, 10 cats and 10 dogs were subjected to laser phototherapy, and the rest were routinely performed as a control group. The clinical and laboratory results of the animals treated with phototherapy were compared by routine applications. During the study, a patient follow-up form was created to record data such as physiological parameters of the animals, wound sizes, and clinical recovery process of the wound. Besides, the data were transferred to the computer environment for statistical analysis. Animals that are too aggressive and do not allow the application was treated with laser phototherapy or dressing by applying a routine sedation or anesthesia protocol with the permission of the owner. The wound was taken in follow-up data daily. For 21 days, wound sizes were drawn on transparencies film. Then these measurements were made using the area calculation graph paper.      In the obtained clinical data, both infection control and wound closure rates were found to be faster in the study groups than the control groups (P<0,05).      In conclusion, low-intensity laser phototherapy has been suggested to be a treatment option, especially in cases of tissue-loss infected skin injuries, which may be an adjunct to classical applications in both cats and dogs.

Open wounds have lost the barrier that protects tissues from bacterial invasion and allowfor the escape of vital fluids. The purpose of this study is to evaluate the enhancing oftherapeutic effect of Low Level Laser (LLL) and chitosan on the acceleration of openwound healing. Forty two adult rabbits were used. They divided into three equal groups (I,II and III). Wound was produced at the dorsal region by remove all thickness of skin atwidth of 3.5cm and of length 4 cm. Group I was left without treatment, while chitosanpowder and laser therapy were used in group II and III respectively. Results of two groupswere compared with control group. Histpathologicl results at the period of 3, 7, 14, 21 and28 days post operation reflected the presence of large number of fibroblast with formationof new blood vessels, also the collagen fiber become dense and regular in 14 days postoperation in irradiated groups. In 21days there was formation of new and normal epidermalclosed the rupture area of III group.

For the induction of arthropathy, 4% hydrogen peroxide (H₂O₂) was injected for 4 weeks into the intra-articular space of the 25 New Zealand white rabbits to damage articular cartilage. The verification of arthropathy induction and the effect of scan-bio laser treatment were determined by measuring superoxide dismutase (SOD) activity, by observing gross and histopathologic findings. The SOD activity increased by about 40% in arthropathy group, as compared to controls.

Twenty-four cross bred adult dogs were separated into four groups and submitted to bilateral neurotomy of the radial nerve, at the level of the distal third of the humerus. In each dog the epineural anastomosis was made 21 days after neurotomy. The surgical region of the left forearm received daily irradiation with GaAs laser for a ten day period in order to evaluate its influence on the repair process. The right forearm was the control. Functional recovery of the forearms was evaluated through ambulation, test of sensibility and motion evaluation. Nerve regeneration was confirmed by histological studies of the anastomosed nervous stumps, collected by biopsy in the 10th day (group A), in the 30th day (group B), in the 60th day (group C) and in the 90th day (group D) after neuroanastomosis. The histological studies showed that the proliferation of the connective tissue on the anastomosis region was the factor that proved to be the greatest impeachment for the nervous regeneration. The GaAs laser radiation decreases the local inflammatory response but may contribute to neuroma formation. | Vinte e quatro cães adultos, sem raça definida, foram separados em quatro grupos e submetidos a neurotomia bilateral do nervo radial, em nível do terço distal do úmero e, 21 dias após, sofreram anastomose epineural secundária, término-terminal. Nos 10 dias subseqüentes à neuroanastomose, o membro esquerdo de todos os cães foi irradiado com laser arseneto de gálio no intuito de investigar sua influência na regeneração do nervo. O membro contralateral serviu como testemunha. A recuperação funcional foi verificada através da característica da deambulação, testes de sensibilidade e avaliação motora. Foram efetuadas biópsias bilaterais, na região de anastomose, aos 10 dias (grupo A), 30 dias (grupo B), 60 dias (grupo C) e 90 dias (grupo D) após a reconstituição cirúrgica, para estudo morfológico em microscopia óptica. A proliferação de tecido conjuntivo na linha de anastomose é a complicação mais séria no processo de regeneração nervosa. A radiação laser diminui a intensidade da resposta inflamatória ao fio de sutura, mas pode contribuir para a formação de neuroma mais exuberante.

Trinta cães sem raça definida foram divididos em três grupos e submetidos à tenotomia e tenorrafia do tendão calcâneo comum direito e esquerdo. No membro esquerdo foi efetuada aplicação laser AsGa (4j/cm2) para avaliar o processo cicatricial dos mesmos. Posteriormente, as articulações do tarso foram imobilizadas durante 11 dias nos animais do grupo I (sacrificados nesta data) e 21 dias naqueles do grupo II (sacrificados aos 22 dias) e III (sacrificados aos 40 dias de evolução pósoperatória). Os cães do grupo III foram submetidos a caminhadas diárias de 30 minutos nas duas semanas seguintes para estimular a mobilidade do tendão. A avaliação clínica dos efeitos da terapia laser na funcionalidade dos membros foi feita através de exames físicos. Os tendões foram avaliados à necropsia através de análise macroscópica e pelo estudo microscópico realizado em todos os segmentos aos 11 (grupo I), vinte e dois (grupo II) e quarenta dias (grupo III) de evolução pós-operatória. O desempenho funcional do membro esquerdo nos animais do grupo III, exposto à radiação laser mostrou-se melhor que no membro direito. Macroscopicamente os tendões irradiados apresentaram melhor vascularização, menos aderências e cicatrização com melhor aparência estética que os tendões testemunhos. Os estudos histológicos revelaram que o laser não interferiu significativamente na produção de fibroblastos e na síntese de fibras colágenas.

<p>In order to evaluate the influence of lasertherapy on the healing process. Thirty crossbred dogs were divided into three groups (group I, II and III: dogs were sacrificed respective at day 10^th, 21^stand 40 ^thafter starting the experiment) and submitted to tenotomy and tenorraphy of the calcanean common tendon of both limbs.Tarsal joints were fixed for eleven days in those dogs of group I and for twenty-one days on group II and III. All dogs from group III were submitted to daily 30 minutes walks for two weeks in order to stimulate the tendon mobility. AsGa laser was applied daily (4j/cm²) during ten days on the left limb of all dogs during the first postoperative days and the first ten days of the exercises period for group III. Clinical evaluation of lasertherapy on limb functionality was made by physical examination. Involved tendons were evaluated at necropsy and by optic microscopy at eleventh post-operative day (group I), twentieth-first day (group II) and fortieth day (group III). Functional performance of the left hind limbs, exposed to the laser beam was better than that of the right limb. Grossly irradiated tendons showed better vascularization, less adherence and better repair than the non-irradiated one. Histological studies showed that laser the rapy did not interfere significantly on fibroblast production and collagem synthesis.</p> | <p>Trinta cães sem raça definida foram divididos em três grupos e submetidos à tenotomia e tenorrafia do tendão calcâneo comum direito e esquerdo. No membro esquerdo foi efetuada aplicação laser AsGa (4j/cm²) para avaliar o processo cicatricial dos mesmos. Posteriormente, as articulações do tarso foram imobilizadas durante 11 dias nos animais do grupo I (sacrificados nesta data) e 21 dias naqueles do grupo II (sacrificados aos 22 dias) e III (sacrificados aos 40 dias de evolução pósoperatória). Os cães do grupo III foram submetidos a caminhadas diárias de 30 minutos nas duas semanas seguintes para estimular a mobilidade do tendão. A avaliação clínica dos efeitos da terapia laser na funcionalidade dos membros foi feita através de exames físicos. Os tendões foram avaliados à necropsia através de análise macroscópica e pelo estudo microscópico realizado em todos os segmentos aos 11 (grupo I), vinte e dois (grupo II) e quarenta dias (grupo III) de evolução pós-operatória. O desempenho funcional do membro esquerdo nos animais do grupo III, exposto à radiação laser mostrou-se melhor que no membro direito. Macroscopicamente os tendões irradiados apresentaram melhor vascularização, menos aderências e cicatrização com melhor aparência estética que os tendões testemunhos. Os estudos histológicos revelaram que o laser não interferiu significativamente na produção de fibroblastos e na síntese de fibras colágenas.</p>