The aim of this study was to compare serum nitrotyrosine concentrations in healthy dogs with those in dogs with myxomatous mitral valve disease (MMVD). Fifty client-owned dogs were included in this study. Based on echocardiographic results, dogs were categorized into healthy (control), mild-, moderate-, and severe-MMVD groups. Serum nitrotyrosine concentrations were determined from enzyme-linked immunosorbent assays. No significant difference between control dogs and dogs with mild MMVD was detected (p = 0.31). However, dogs with moderate MMVD had significantly higher serum concentrations of nitrotyrosine (p = 0.04) than that in controls, and dogs with severe MMVD had significantly lower serum concentrations of nitrotyrosine (p = 0.03) than that in moderate MMVD dogs. There were negative correlations in the association of serum nitrotyrosine with age (n = 30, R2= 0.067, p = 0.27), left atrial-to-aortic root diameter ratio (n = 30, R2= 0.02, p = 0.57), and platelet count (n = 30, R2= 0.39, p = 0.003); however, only the platelet correlation was significant. Among dogs with MMVD, there was no significant difference in serum nitrotyrosine concentration between males and females. The results of this study suggest that tyrosine nitration end-products might be potential biomarkers for the detection of MMVD in dogs.

In the conditions of the Republic of Belarus there was determined the therapeutic efficacy of combined application of a complex antibacterial veterinary drug (containing lincomycin and spectinomycin) and Trivitim for the treatment of calves with respiratory diseases. In course of the study of 12 1-1,5 month old calves with respiratory diseases there were analyzed the hematological blood indexes, white blood cell differential and content of total protein in blood serum before the beginning of treatment and on the seventh and fourteenth days after beginning treatment. Research results showed that application the antibacterial veterinary drug in complex therapy with Trivitim in treatment of calves proved to be efficient. Their administration shortened the terms of recovery and prevented animal death as well as promoted the normalization of hematological and biochemical blood indexes.

Results of serological examinations of blood serum of sheep on revelation of virus respiratory infections (parainfluenza-3, adenovirus and respiratory syncytial) realized in the conditions of the Kyrgyz Republic were presented. Research results showed that in farm and peasant farm holdings of all studied districts of the Kyrgyz Republic of three types of pneumo viral infections simultaneously give high levels of antibody titers in relation to parainfluenza-3 – 1:128, adenovirus – 1:32, respiratory syncytial infection – 1:16. In some districts sheep population was diseased only by parainfluenzal infection in relation with titer of specific antibodies 1:256

The article features the data on scientific research of the spread and etiology of bovine dermatophytoses. Work was carried out in 2007-2009 in farm holdings of Minsk and Vitebsk regions of the Republic of Belarus. Principal causes of diseases occurrence are the mechanical traumas caused first of all by infringement of animals maintenance of hygienic norms, also infringement of animals feeding technology. The automicroflora on intact skin has been studied. Realized study helped to determine methods and means of dermatophytoses treatment.

The realized studies of an experimental series of inactivated vaccine showed that the developed biological preparation made it possible to achieve high preventive efficiency in the conditions of cows heavy with calves for the creation in newborn calves the expressing immunity after milk feeding in the first hours of their life. As a result realized in the conditions of the Republic of Belarus production experiment of emulsified vaccine against rotavirus infection and colibacillosis of cattle in animal breeding farms of different regions of the Republic the prophylactic efficacy of a developed vaccine made up 97,9-98,9%. Research results showed that the developed inactivated vaccine caused at the immunizated animals the formation of specific antibodies at a higher level in comparison with other analyzed national and foreign analogue preparations.

Research results showed that in the conditions of correct organization and application of technological methods of livestock breeding and feeding it was possible to achieve high livability rate of sheep and lambs up to 90%. Also it was possible to get a substantial break of infection chain of many sheep diseases (especially those which had a respiratory nature). Research results showed that simultaneous application of convalescent-phase serum against sheep acute respiratory diseases, as well as Nitox 200 and Invamek for treatment of sheep and especially 2-3 month old lambs with respiratory infectious diseases promoted the recovery of 80-100% of animals.

Analysis of national studies and literary sources on problems of haemophilus pleuropneumoniae of swine was realized in the conditions of the republic of Belarus. Research was realized in the conditions of Agricultural Production Co-operative Uritskoe (Gomel region, Belarus). It was established that medical actions gave positive result only at early stages of disease.

Definition of quality parameters and chemical composition of feeds, evaluation their nutritional value and composing on this basis optimal rations for high productive animals are the most significant conditions for their health and production. More often metabolism disorders registers in the conditions of intensive animal production, at high dairy efficiency and intensive fattening. At animals at disorder of key parameters of technology (unbalanced feeding, hypodynamia, lack of insolation) is broken metabolism, decreases natural resistance, goes down quality of production that conducts to disease, premature culling and to the compelled slaughter of animals. On frequency these diseases occupy on the average 10% from all noncontagious diseases, and on dairy complexes with industrial technology and in specialized feeding economy at intensive cultivation on their share it is necessary over 30% of a noncontagious pathology. Today the control of rations on such indicators, as dry matter level, quantity of exchange energy, the maintenance of a crude and digested protein, amino acids, sugars, starch, cellulose, fat, macro-and microelements, vitamins, and also sugar-energy-protein and calcium-phosphoric parities, acid-base balance has got special value. The lack of those or other nutrients causes infringement of a clinical condition of animals and reduces their efficiency. Among the most widespread diseases of the animals registered in animal industry: cattle ketosis, osteodystrophy, endemic struma, hypomagnesiemia tetany, pasturable tetany, microelementosis, hypovitaminoses A and Е.

In the conditions of the Republic of Belarus there was produced a trial series of hyperimmune serum against livestock animals colibacillosis and there was realized the quality control of the analysed biological preparation. Research results proved that the developed serum was sterile, safe and had high immunogenetic activity. In course of quality control there were considered the following indexes: determination of physiognomy, colour, presence of visually accessible particulate matters; determination of safety and harnfullness; determination of activity; determination of concentration of hydrogen ions; determination of sterility; determination of serum volume in consumption package. For serum production there were used strains E.coli O9, O78, O0, O117, O26, O139, O15, O41, O55, O115, O141, O8, K88, K99, F41, 987P. For the production of trial series there was realized cultivation of Escherichia on the special nutritive media. There used bulls as serum producers

Analysis of data on distribution of clinicopathologic symptoms of rhinotracheitis, parainfluenza-3, bovine viral diarrhoea, clamidiosis, rhinosyncytial virus, adenovirus diseases, rotaviral diseases, coronavirus infections of cattle, viral transmissible gastroenteritis, and respiratory reproductive syndrome of swine, rinderpest, rabies and Aujesky disease of agricultural animals in the conditions of the Republic of Belarus was realized. There were given descriptions of diagnostic tests and vaccines for disease prevention used in Belarus and other countries. Treatment regimen and methods and other elements of prophylaxis were presented. There was given an analysis of prophylactic efficiency of produced medicine drugs