Male factor infertility contributes partially and solely to the problem of childlessness inaround 50% of the cases. Unfortunately, 30 -- 50% of the etiologies of male infertility are unknownand therefore, no specific therapy can be instituted. Sperm count and sperm motility are primeparameters that determine the functional ability of spermatozoa.This study included determinationof lactate dehydrogenase-C(LDH) activity in male infertility (n=23)and its relationship with thedifferent parameters of the seminogram. Also deals with partial purification lactatedehydrogenasefrom semen plasma of male infertility patients by dialysis, and ion exchange DEAEcellulosetechniques. There was statistically significant correlation was found between LDH activityand Sperm Count , morphology % and Motility % (r=0.486), (r=0.263)and (r=0.444) in fertile grouprespectively, also that the correlation in infertile group was statistically significant between LDHactivity and Sperm Count , morphology % and Motility % (r=0.431), (r=0.671)and (r=0.336)respectively.Oneproteinous peak of lactate dehydrogenaseactivity is obtained with specific activity of 0.861u\mgprotein and purification fold 6.10 compared to crude enzyme. The kinetic characteristics of partiallypurified lactate dehydrogenaseare studied. The maximum activity is obtained at 250μg of enzyme,Tris-Hcl buffersolution at pH 7.9, temperature 37ᵒC, incubation time 5 min., with 10mMK+, Vmax and Km values of partially purified lactate dehydrogenase1.176 unit/ml and 27.02 mM with thesubstrate sodium pyruvateare found respectively

Objective: This study aimed to investigate the impact of thioacetamide (TAA) on the structure and function of the testes and assess the therapeutic effects of Curcuma longa (Cl) against TAA-induced toxicity in rats. Materials and Methods: Thirty-two male albino rats weighing 180–200 gm and aged 11–12 weeks were randomly separated into four groups. The control group was given normal saline, the Cl group was orally administered Cl (500 mg/kg/day), the TAA group received intraperitoneal TAA (200 mg/kg body weight, three times/week), and the Cl with TAA group received Cl orally two hours before TAA administration. After 8 weeks, all rats were anesthetized, and body and testis weights were recorded. Morphological and histological assessments as well as biochemical analyses were conducted. Results: The study revealed a significant decrease in both body and testis weights in the TAA group, accompanied by a substantial increase in luteinizing hormone (LH), follicle-stimulating hor¬mone (FSH), and malondialdehyde (MDA) levels. Testosterone (T) and glutathione (GSH) were significantly decreased in the TAA-treated group compared to the control. Conversely, the Cl-treated group exhibited a substantial decrease in LH, FSH, and MDA levels while showing a significant increase in T and GSH. Conclusion: Cl has been found to have a potential therapeutic role in mitigating TAA-induced testicular damage by acting as an antioxidant. This is supported by a significant decrease in oxi¬dative stress markers and supporting hormonal levels. Further research is needed to understand the underlying mechanisms and explore the clinical applicability of Cl in preventing and treating testicular toxicity. [J Adv Vet Anim Res 2024; 11(3.000): 762-771]