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The effect of green tea on ultraviolet B-induced sunburn cell production in the skin of hairless mouse
2005
Kim, S.H. (Chonnam National University, Gwangju, Republic of Korea), E-mail: shokim@chonnam.ac.kr | Jang, J.S. (Sangju National University, Sangju, Republic of Korea) | Jo, S.K. (Korea Atomic Energy Research Institute, Daejeon, Republic of Korea) | Kim, S.R. (Chonnam National University, Gwangju, Republic of Korea) | Lee, H.J. (Chonnam National University, Gwangju, Republic of Korea) | Lee, J.H. (Chonnam National University, Gwangju, Republic of Korea) | Kim, Y.J. (Chonnam National University, Gwangju, Republic of Korea) | Kim, J.C. (Chonnam National University, Gwangju, Republic of Korea)
In this study we assessed the influences of ultraviolet (UV) light B radiation on epidermal cells by apoptotic sunburn cell (SBC) and the effect of green tea treatment on the inhibition of SBC formation in SKH1-hr mouse. The extent of changes following 200 mJ/㎠ (0.5 mW/sec) was studied at 0, 3, 6, 12, 18, 24, 30 or 36 hours after exposure. SBCs were recognized by 3 hours after irradiation. There was tendency to increase from 3 hours to 24 hours and decrease from then to 36 hours after irradiation. The mice that received 0, 25, 50, 100, 200, 400 or 800 mJ/㎠ of UVB were examined 24 hours after irradiation.
Показать больше [+] Меньше [-]The study for the mouse cerebellum developments irradiated with γ-ray during embryogenesis
2005
Park, I.K. (Chungnam National University, Daejeon, Republic of Korea) | Lee, G.J. (Chung Nam Veterinary Service Laboratory, Daejeon, Republic of Korea) | Lee, K.Y. (Daejeon University, Daejeon, Republic of Korea) | Min, T.S. (Korea Science and Engineering Foundation, Daejeon, Republic of Korea) | Byun, M.W. (Korea Atomic Energy Research Institute, Daejeon, Republic of Korea) | Lee, K.Y. (Chungnam National University, Daejeon, Republic of Korea) | Park, O.S. (Chungnam National University, Daejeon, Republic of Korea) | Kim, S.H. (Chungnam National University, Daejeon, Republic of Korea) | Yook, H.S. (Chungnam National University, Daejeon, Republic of Korea) | Kim, M.K. (Chungnam National University, Daejeon, Republic of Korea), E-mail: mgkim@cnu.ac.kr
In order to study about the lobule and layer formation and cell migration of the mouse cerebellum from at the birth to 15th day effected by 2.5, 5 and 10 Gy r-raddiation at the 19th pregnancy. The routine tissue preparation and staining procedure, Immunohistochemical staining method by the several antibody and western brotting method were utilized from the birth to the 15th day. The results were as followings. 1. The body and cerebellum weights were more slowly increase of the the 2.5 Gy, 5 Gy and 10 Gy irradiation group compare to the control group, and the health condition of the 2.5 Gy group was a little bad. but the 10 Gy group was more severe and begun to die from the 12th day after birth.
Показать больше [+] Меньше [-]Examination of the Lunatic fringe and Uncx4.1 expression by whole-mount in situ hybridization in the embryo of the CKH-Jsr (jumbled spine and ribs) mouse
2005
Okano, S. (Hokkaido Univ., Sapporo (Japan)) | Asano, A. | Sasaki, N. | Kon, Y. | Watanabe, T. | Agui, T.
The CKH-Jsr (jumbled spine and ribs) mouse was found as a spontaneous mutant with malformation of vertebrae, that is, a short trunk and kinky tail. We examined Lunatic Fringe (Lfng and Uncx4. 1 expression in the presomitic mesoderm (PSM) and somites of Jsr-mutant (CKH-Jsr/+) embryos to elucidate pathogenesis of the Jsr mutation. Expression pattern of Lfng in the PSM of Jsr-mutant embryos was similar to that of the normal (C57BL/6) embryos. However, expression pattern of Uncx4. 1 in the somites of Jsr-mutant embryos was impaired to be irregular and mosaic, suggesting that the anterior-posterior (A-P) polarity is disordered in the Jsr mutant. These results indicate that the Jsr mutation disrupts the A-P polarity of somites during the somitogenesis without altering Lfng expression pattern in the PSM.
Показать больше [+] Меньше [-]Upregulation of renal renin-angiotensin system in mouse diabetic nephropathy
2005
Tamura, J.(Hokkaido Univ., Sapporo (Japan)) | Konno, A. | Hashimoto, Y. | Kon, Y.
The aim of this study was to clarify the role of the renal renin-angiotensin system (RAS) in diabetic nephropathy (DN) , which was induced by injection of streptozotocin (STZ). Male CBA/N and CBA/J mice were compared in this study. The former possesses a single renin gene, Ren1. whereas the latter carries two renin genes, Ren1 and Ren2. To examine the molecular dynamics of renal RAS, including renin, angiotensinogen (Agt), angiotensin-converting enzyme (Ace), angiotensin type 1 (Agtr1) and type 2 (Agtr2) receptors in experimental DN, we performed laser-microdissection (LMD) followed by reverse transcriptase nested polymerase chain reaction using each specific primer pairs and immunohistochemistry for renin and angiotensin 2. CBA/N mice had a higher response after injection of STZ than CBA/J mice, showing a significant increase of the kidney/body weight ratio, although there was no significant difference between the two strains for the blood glucose level or pancreatic beta-cell response. The onset of renal pathological changes associated with DN was earlier and more severe in CBA/N mice than in CBA/J mice. Distinct immunoreactivities for renin and angiotensin 2 were newly distributed on the flattered epithelial cells in the dilated distal tubules in the cortex as well as the collecting ducts in the cortex and medulla, and were demonstrated more intensity in CBA/N mice than in CBA/J mice. Micro dissectional analysis in both models revealed a higher incidence of RAS-related gene expression in CBA/J, Ren 2 mice than in CBA-N, Ren 1 mice.
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