This study was planned to evaluate the extracts of Aloe vera (AV) at three different pH as a growth promoter, immunostimulant and coccidiostat agent in broiler production. This experiment was undertaken for a period of 35 days in the Institute of Animal Sciences, Faculty of Animal Husbandry, University of Agriculture Faisalabad, Pakistan. A total of 150 dayold broiler birds (Cobb) were randomly assigned to 5 treatments with 3 replicates of 10 chicks each. Birds were offered commercial broiler starter and finisher diets supplemented with 100 mg/kg enramycin (ANT), 50 ml/liter AV of pH 3 (AV1), 50 ml/liter AV of pH 7 (AV2), 50 ml/liter AV of pH 12 (AV3) and only fresh clean water (CON).

Newcastle and Influenza diseases are important viral diseases and its occurrence and virulence in Iran has increased in recent years. The purpose of this study was to evaluate the humoral immune responses of chickens vaccinated with inactivated dual oil emulsion Newcastle disease and avian influenza vaccine in two methods of intramuscular and subcutaneous injection as well as to assess the possible changes in serum biochemical factors. In this study, after subcutaneous and intramuscular inoculation of inactivated dual oil emulsion Newcastle-Influenza vaccine, the serum antibody level and hematobiochemical factors of these avian were analyzed on 7, 14, 21, and 28 days after vaccine injection.The results showed that the values of biochemical parameters such as albumin, glucose, total protein, triglyceride, cholesterol, ALT, AST, and ALP enzymes, sodium and potassium minerals, and hematocrit and hemoglobin levels in vaccinated and non-vaccinated chickens were not significantly different. Also, in chickens injected with antigen, Newcastle disease and influenza antibody titers were significantly different with those groups containing adjuvant.The results of this study indicate that the intramuscular and subcutaneous injection of dual combination vaccine is similar in terms of changes in biochemical and hematological factors and Newcastle disease and influenza antibody titers. To ensure safety in inactivated vaccines, the presence of proper adjuvant is essential for immune response.