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Detection of viral antigens in bluetongue virus-infected ovine tissues, using the peroxidase-antiperoxidase technique.
1985
Cherrington J.M. | Ghalib H.W. | Sawyer M.M. | Osburn B.I.
Observations on transplacental infection with bluetongue virus in sheep.
1985
Richardson C. | Taylor W.P. | Terlecki S. | Gibbs E.P.J.
Immunologic response of sheep to inactivated and virulent bluetongue virus.
1985
Stott J.L. | Barber T.L. | Osburn B.I.
Humoral immune response of calves to bluetongue virus infection.
1987
MacLachlan N.J. | Heidner H.W. | Fuller F.J.
The sero-prevalence and sero-incidence of African horse sickness and equine encephalosis in selected horse and donkey populations in Zimbabwe
2017
Stuart J.G. Gordon | Charlotte Bolwell | Chris W. Rogers | Godfrey Musuka | Patrick Kelly | Alan Guthrie | Philip S. Mellor | Christopher Hamblin
Sentinel herds and samples submitted by private equine practitioners were used to determine the sero-prevalence and sero-incidence of African horse sickness virus (AHSV) and equine encephalosis virus (EEV) in horse and donkey populations in the Highveld region of Zimbabwe. The sero-prevalence and sero-incidence of antibodies against these viruses were determined using the competitive enzyme-linked immunosorbent assay (ELISA) for the detection of serum antibodies. In donkeys, the median sero-prevalence of AHSV antibodies, across the three rainy seasons under study, was 75% (inter quartile range [IQR] 67–83), with a seasonal median sero-incidence of 45% (IQR 40–63). In horses, the median sero-prevalence of EEV antibodies was 63% (IQR 21–73), with a median seasonal sero-incidence of 10.5% (IQR 10–14), while in donkeys the median sero-prevalence of EEV antibodies was 80% (IQR 67–90), with a median seasonal sero-incidence of 50% (IQR 40–60). This study highlighted the significant levels of exposure of donkeys to AHSV and horses and donkeys to EEV in Zimbabwe despite equine encephalosis remaining unreported by Zimbabwean veterinarians to date. Most seroconversions in sentinel herd animals to AHSV and EEV occurred towards the end of the rainy season in March, April and May corresponding to the time of the year when the Culicoides vectors are in high abundance. In order to determine the clinical significance of these infections, blood and spleen samples, submitted by private equine veterinary practitioners over a 5-year period, from horses showing characteristic clinical signs of African horse sickness were tested for the presence of viral antigen using the antigen capture ELISA. The median sero-prevalence of AHSV antigen in horses recorded from these samples was 38% (IQR 33–88). The predominant AHSV antigen from these samples was serotype 7 (33%) followed by serotype 2 (26%) and serotypes 4 and 8 (16% each). African horse sickness virus serotypes 3 and 9, identified in this study, had not been previously reported in Zimbabwe.
Показать больше [+] Меньше [-]Prevalence of serotype specific antibody to equine encephalosis virus in Thoroughbred yearlings in South Africa (1999-2004)
2008
Howell, P.G.(University of Pretoria Faculty of Veterinary Science Equine Research Centre) | Nurton, Jane P.(University of Pretoria Faculty of Veterinary Science Equine Research Centre) | Nel, Daleen(University of Pretoria Faculty of Veterinary Science Equine Research Centre) | Lourens, Carina W.(University of Pretoria Faculty of Veterinary Science Equine Research Centre) | Guthrie, A.J.(University of Pretoria Faculty of Veterinary Science Equine Research Centre)
Cohorts of yearlings were sampled over a period of 6 years in a retrospective serological survey to establish the annual prevalence of serotype specific antibody to equine encephalosis virus on Thoroughbred stud farms distributed within defined geographical regions of South Africa. Seasonal seroprevalence varied between 3.6 % and 34.7 %, revealing both single and multiple serotype infections in an individual yearling. During the course of this study serotypes 1 and 6 were most frequently and extensively identified while the remaining serotypes 2, 3, 4, 5 and 7 were all identified as sporadic and localized infections affecting only individual horses. This study of the seasonal prevalence of equine encephalosis virus has a corollary and serves as a useful model in the seasonal incidence of the serotypes of African horse sickness and bluetongue in regions where the respective diseases are endemic.
Показать больше [+] Меньше [-]Isolation and characterization of epizootic hemorrhagic disease virus from sheep and cattle in Colorado
1988
Thompson, L.H. | Mecham, J.O. | Holbrook, F.R.
Epizootic hemorrhagic disease virus was isolated from cattle and sheep in northeastern Colorado during July and August 1984. The isolates were identified as serotype 2 by plaque-inhibition serotyping, genome electropherotyping, and protein analysis.
Показать больше [+] Меньше [-]Bluetongue virus infection in pregnant ewes
1994
Parsonson, I.M. | Luedke, A.J. | Barber, T.L. | Walton, T.E.
Inoculation of 53 ewes after 35, 45, 60, or 80 days of gestation with bluetongue virus serotypes 10, 11, 13, or 17, or with epizootic hemorrhagic disease virus serotypes 1 or 2, resulted in overt clinical disease in the 47 ewes inoculated with bluetongue virus but not in the 6 ewes inoculated with epizootic hemorrhagic disease virus. None of the lambs produced by these ewes had developmental defects or any evidence of persistence of viremia.
Показать больше [+] Меньше [-]Prevalence of bovine herpesvirus-1, parainfluenza-3, bovine rotavirus, bovine viral diarrhea, bovine adenovirus-7, bovine leukemia virus and bluetongue virus antibodies in cattle in Mexico
1983
Suzan, V.M. | Aguilar, R.E. (Direccion General de Sanidad Animal, Piso (Mexico)) | Onuma, M. | Murakami, Y.
The classification of seven serotypes of equine encephalosis virus and the prevalence of homologous antibody in horses in South Africa
2002
Howell, P.G. (Pretoria Univ., Onderstepoort (South Africa). Equine Research Centre) | Groenewald, D. | Visage, C.W. | Bosman, A.-M. | Coetzer, J.A.W. | Guthrie, A.J.