Eight rabbits exhibited head tilt and subsequently died. At necropsy, three rabbits had crusty deposits in ears and four had reddish lungs. The main histopathological features were severe diffuse suppurative meningoencephalitis (75.0% of rabbits), fibrinopurulent pneumonia (37.5%), and otitis externa (37.5%). Pasteurella multocida (P. multocida) was isolated from brains, ears, and lungs. The capsular serogroups of the isolates were untypable. Based on histopathological features and bacterial analysis results, the rabbits were diagnosed as P. multocida infection. P. multocida infections might result in considerable economic loss in commercial rabbit production facilities in Korea.

Research of immune activity of an experimental series of inactivated vaccine against swine pasteurellosis (Pasteurella) with application of adjuvants of Sepptic Montanida ISA 70 and Montanida ISA 206 was realized in the conditions of the Republic of Belarus. In the capacity of water phase there were used serovars A, B, and D Pasteurella multocida. Research results proved the possibility of application of adjuvants of Sepptic company for the development of vaccine against pasteurellosis. The vaccine with the presented adjuvants after its application did not cause after-trouble effects. In course of immunization there was noted the formation long-lived continuous immunity that was proved by high titres of specific anti-bodies in blood serum in all experimental groups of piglets. Single application of the proposed vaccine in doses 2 or 3 ml per animal proved to be effective. Also it was efficient to apply it twice in doses 1 and 2 ml per animal. For the vaccine production it was proposed to use adjuvant Montanida ISA 206 which did not have high reactogenicity

Morphological indexes in immune system organs of piglets which were vaccinated which were immunized by SPS vaccine (against salmonellosis (Salmonella), pasteurellosis (Pasteurella) and streptococcus) in combination with immunostimulative drugs and without them were studied in the conditions of the Republic of Belarus. Piglets of the first group were vaccinated against salmonellosis, pasteurellosis and streptoccocusis by SPS vaccine. Piglets of the second group were vaccinated by SPS vaccine in combination with vitamin C. Piglets of the third group were vaccinated by SPS vaccine with immunotstimulant sodium thiosulphate. The fourth (control) group of animals was administrated with normal saline. Animals were immunized twice, intramuscularly with 7 day interval and in dose 4 ml (primary), 5 ml (secondary). Vitamin С was added in dose 0,05g per head. Research results showed that immunization by SPS vaccine with sodium thiosulphate and Vitamin С activated the morphological reaction of immune system. Application of Vitamin С and sodium thiosulphate (30% concentration) in combination with SPS vaccine promoted the increasing of lymphoid nudels in lymphodnudus and lien, increasing of plasma cells quantity in 1,3-2,2 times, activation of micro- and macrophage reaction in 1,5-2,8 times in comparison with vaccinated animals

Among bacterial infections of fur animals in beast breeding farm in Belarus pasteurellosis takes a leading place - 42,06%. Pasteurellosis of fur animals is characterized by the expressed seasonal prevalence. The greatest percent of Pasteurella allocation is registered during the autumn and spring periods. The maximum allocation is noted in October and March accordingly 18,55% and 13,21%. Infection rate with pasteurellosis depends on age of fur animals. The most susceptible is the young growth at the age from 2 till 5 months. Pasteurellosis of fur animals is most often clinically shown by symptoms of defeat of respiratory organs and has subacute and chronic current. Pathoanatomical changes at fur animals at pasteurellosis have expressed tropism to respiratory system with formation of characteristic changes without dependence from a disease current; at acute process the picture of hemorrhagic diathesis is added, at a chronic current are observed necrotic changes in a number of tissues. | Среди бактериальных инфекций пушных зверей в звероводческих хозяйствах Республики Беларусь пастереллез занимает ведущее место - 42,06%. Пастереллез пушных зверей характеризуется выраженной сезонностью. Наибольший процент выделения пастерелл регистрируется в осенний и весенний периоды. Максимальное выделение отмечено в октябре и марте соответственно 18,55% и 13,21%. Уровень заболеваемости пастереллезом зависит от возраста пушных зверей. Наиболее восприимчивым является молодняк в возрасте от 2 до 5 месяцев. У пушных зверей пастереллез наиболее часто клинически проявляется симптомами поражения органов дыхания и имеет подострое и хроническое течение. Патологоанатомические изменения у пушных зверей при пастереллезе имеют выраженный тропизм к дыхательной системе с формированием характерных изменений вне зависимости от течения заболевания; при остром процессе добавляется картина гемораггического диатеза, при хроническом течении наблюдаются некротические изменения в ряде тканей.

In the conditions of the Republic of Belarus there was studied sterility, harmlessness and immunoactivity of an obtained production vaccine against swine pasteurellosis by an example of laboratory animals – white mice and rabbits. Research results showed that the obtained vaccine was sterile and harmless for the laboratory animals, and had a high immunogenic activity. The analyzed vaccine prevented disease incidence of white mice after its double application in dose of 0,1 ml per animal and after a single immunization of mice in dose of 0,2 ml per animal. The inactivated vaccine caused the formation of immunity of rabbits after double immunization in dose of 1 ml per animal with 14 days interval and provided 100% vitality of animals.

In the conditions of the Republic of Belarus there was realized a cultivation of pasteurellas by different methods in Hottinger broth in 37 deg C temperature in course of 24 hours. Tissue culture properties were studied in accordance with the character of bacteria growth in liquid nutritive media and at the agar surface. Research results showed that in 7 hours after cultivation in media with application of shuttel apparatus and addition of glucose it was possible to obtain the highest quantity of viable pasteurellas with a high degree of virulence. The obtained culture on the basis of obtained results was proposed to be used for the production of virulence antigen.

Experimental research on production of antigen preparation for hyperimmunization of bulls from blood of which it was possible to produce an active medical and preventive serum against cattle salmonellosis and pasteurellosis was realized in the conditions of the Republic of Belarus. There were developed four variants of antigen on the basis of the formolated aluminous concentrated vaccine against calf salmonellosis and on the basis of semiliquid aluminum hydroxide vaccine against cattle pesteurellosis. All obtained variants of associated antigen had concentration of hydrogen ions, which was close to the neutral rating; they were also sterile and safe for white mice. Associated antigen which was constructed from vaccines in the ratio of 1:1, 1:2, 1:3, and 2:3 had an ability to raise the protection of immunized experimental guinea pigs in the conditions of controlled introduction of infection with S. dublin 373 and S. tythimurium 371. Pasteurellosis component of associated antigen in 1:1 and 1:2 variants protected against death only 3 out of 5 immunized doves, both in relation to P. multocida strain N 796, and bacteria P. multocida strain N 5264. Vaccine ratio in the associated antigen 1:3 and 2:3 turned to be more immunogenically balanced: all tested doves survived after introduction of infection of broth culture P. multocida of N 796 and N 5264 strains. Experimental results showed that the associated antigen on the basis of vaccines in ratio of 1:3 was sterile, safe, active and suitable for hyperimmunization of bull producers serum against salmonellosis and pasteurellosis of calves, as well as antigen in the variant 2:3, but this ratio increased the expenses

Morphological and structural changes in bone marrow of piglets which were immunized with produced in the Republic of Belarus SPS vaccine (against salmonellosis (Salmonella), pasteurellosis (Pasteurella) and streptococcus) in combination with immunostimulants and without them were analyzed in the conditions of the Republic of Belarus. Piglets of the first group were vaccinated against salmonellosis, pasteurellosis and streptoccocusis by SPS vaccine. Piglets of the second group were vaccinated by SPS vaccine with vitamin C. Piglets of the third group were vaccinated by SPS vaccine with immunotstimulant sodium thiosulphate. The fourth (control) group of animals was administrated with normal saline. Animals were immunized twice, intramuscularly with 7 day interval in dose of 4 ml (primary), and 5 ml (secondary). Vitamin С was administrated in dose of 0,05g per head. Sodium thiosulfate was administrated in combination with the vaccine in 30% concentration. Alongside with myelogram there were derived formulas of different cell groups of bone marrow: leucoerythroblastic index; intramedullary index of neutrophil maturity; intramedullary index of eosinophile maturity. Detailed myelogram of piglets on the 14-th day after the second immunization was presented. Research results showed that in all groups of vaccinated animals there was noted the activation of myeloblastic hematogenesis and decreasing of erythropoeisis. Piglet immunization was accompanied by strengthening of myeloblastic hematogenesis, increased number of lymphocytes and plasma cells in bone marrow

The results of Pasteurella allocation from fur animals on the basis of studying of their cultural-morphological, biochemical properties and definition of a specific accessory are given. As a result of carrying out of bacteriological research of the pathological material received from fur animals, pure cultures have been allocated. On the basis of cultural-morphological and biochemical properties these cultures are carried to Pasteurella multocida. At serotyping these cultures Pasteurella multocida are carried to serovariants A, B and D. For selection the most important in the epizootic relation serovariants A and B Pasteurella multocida have been selected. Selected strains of serovariants A and B Pasteurella multocida had stable properties for everyone serovariant Pasteurella, that has given the basis to passport them in a collection of microorganisms to The S.N.Vyshelesskij Institute of Experimental Veterinary Medicine and a demand tax in the National centre of intellectual property at Ministerial council of Belarus on patents | Приводятся результаты выделения от пушных зверей пастерелл на основании изучения их культурально-морфологических, биохимических свойств и определение серовариантной принадлежности. В результате проведения бактериологических исследований патологического материала, полученного от пушных зверей, были выделены чистые культуры на основании культурально-морфологических и биохимических свойств и отнесены к Pasteurella multocida. При серотипизации установленные культуры Pasteurella multocida отнесены к серовариантам А, В и D. Для проведения селекции были отобраны наиболее важные в эпизоотическом отношении серовариан-ты А и В Pasteurella multocida. Селекционированные штаммы серовариантов А и В Pasteurella multocida имели стабильные свойства для каждого сероварианта пастерелл, что дало основание паспортизировать их в коллекции микроорганизмов Инстита экспериментальной ветеринарии им. С.Н. Вышелесского и подать заявки в Национальный центр интеллектуальной собственности при Совете Министров Республики Беларусь на патенты

The data about influence of the developed home-produced inactivated vaccine against pasteurellosis of fur animals on blood, biochemical, immunity indicators at mink are cited. It is established, that application of the given preparation conducts to formation of the necessary immune answer. The developed domestic inactivated vaccine against pasteurellosis of fur animals is a harmless and areactogenic preparation. This vaccine does not render negative action on hematological and biochemical indicators of mink blood. They are identical to indicators in control group of fur animals. Immunization with this vaccine stimulates cellular and humoral immunity factors that testifies to formation of the proof immune answer | Приведены данные о влиянии разработанной отечественной инактивированной вакцины против пастереллеза пушных зверей на гематологические, биохимические, иммунологические показатели норок. Установлено, что применение данного препарата ведет к формированию необходимого иммунного ответа. Разработанная отечественная инактивированная вакцина против пастереллеза пушных зверей является безвредным и ареактогенным препаратом. Инактивированная вакцина против пастереллеза пушных зверей не оказывает отрицательного действия на гематологические и биохимические показатели крови норок. Они идентичны показателям в контрольной группе пушных зверей. Иммунизация инактивированной вакциной против пастереллеза пушных зверей стимулирует клеточные и гуморальные факторы иммунитета, что свидетельствует о формировании стойкого иммунного ответа