A disease characterized by papules, nodules, vesicles, pustules and ulcers on teats and udder as well as drastic drop in milk production was seen among a cattle farm in Fayoum Governorate, Egypt. A virus was isolated by inoculation of vesicle and scrap homogenate pool from infected cattle into the chorioallantoic membrane of specific pathogen free embryonated chicken eggs. The virus was identified by presence of pock lesions, intracytoplasmic inclusion bodies on the chorioallantoic membrane, polymerase chain reaction and immunohistochemistry of the inoculated membrane. A novel pathogenicity model was developed via ear pinna inoculation of Swiss mice. The virus produced vesicular and ulcerative lesions at the site of inoculation in inoculated mice. The virus identity was confirmed by the presence of intracytoplasmic viral antigens by immunohistochemistry

This study was conducted to investigate the pathogenicity of Paenibacillus (P.) polymyxa JB115 and single oral dose toxicity of culture broth containing β-glucan (CBG-JB115) produced from P. polymyxa JB115 in Sprague-Dawely rats of both sexes for 14 days. After oral administration of P. polymyxa JB115 into rats, we could not find any abnormal clinical signs and variation in the body weight and temperature as compared with control group. We also investigated the acute toxicity of CBG-JB115. As the results, there were no clinical signs and variance in the body weight and temperature related with CBG-JB115 in comparison with the control group.