A 2-year-old male stray dog was found to be bleeding from the penile sheath for two months. Physical examination by penile extrusion revealed a severe proliferative and hemorrhagic mass at the bulbar glandis. A natural product ointment was applied in an aerosol spray weekly to evaluate the progress of tumour regression. The reduction in size of the mass at the bulbar glandis was observed within seven days of treatment with the spray of a natural product, namely a liquid mixture of Catharanthus roseus and Cynodon dactylon. Complete resolution of tumor mass was achieved in 4 weeks. The bleeding from the mass was halted within 24 hours of first sprayingwhich exhibited a potent anti-inflammatory effect of local herbs. This preliminary study was conducted to establish the potential use of herbal plants for routine non-invasive treatment of transmissible venereal tumor in a male dog.

A number of studies have measured collagen fibers and collagen
deposition in wound healing process with advances imaging techniques. However, these are performed by complicated methods and need specific tools. In search of the easier ways in routine histopathological laboratory, collagen measurement and staining pattern of wound healing process were observed in wounded skin of Sprague Dawley’s rat by using two different stains which are standard haematoxylin and eosin (H&E) and modified Masson’s
trichrome staining (MT). The comparison between these staining in wounded tissues was made to evaluate the advantages and disadvantages of both staining in wound healing study for 21 days postwounding. Tissues which stained with MT staining was then evaluated its collagen re-organization and density by using polarized light microscope with the aid of image analyzer software. Results showed that tissues stained with standard H&E could not be used to measure and differentiate the collagen deposition which is contradictory to MT staining. Wounded tissue stained with MT staining has showed a clear view of collagen fibers deposition
and re-organisation compared to H&E staining. This finding could validate the using of modified MT staining which leads to accurate histopathological analysis and observation in wound healing study.