A total of 23,322 specimens collected between 2014 and 2017, froma total of 2,592 cases, were received in Veterinary Research Institute, Ipoh (VRI) from various states in Malaysia and testedfor common bacterial, viral, and parasitic diseases in pigs. The highest occurrence of isolated bacteria from 771 samples whichtested positive were Salmonella (47.38%) and Escherichia coli (15.68%), followed by Staphylococcus (6.62%), Streptococcus (5.57%), Klebsiella pneumonia (4.88%), Pseudomona (3.38%), Acinetobacter (3.14%), Aeromonas (2.79%), Enterobacter (2.44%), one each of Bacillus and Pasteurella multocida (1.74%), Enterococcus (1.39%) and Corynebacterium (1.05%). 1.74% of each bacteria detected were Moxarella, Aspergillus, Burkholderia andChromobacterium. Positive samples tested by ELISA was Japanese encephalitis virus (JEV) (9.15%), Aujezsky disease virus (ADV)(5.37%), porcine cirvo-virus-2 (PCV2) (5.09%) and porcine reproductive and respiratory syndrome virus (PRRSV) (4.52%). Positive amples tested by the molecular test wasPCV2 (1.62%), PRRSV (1.32%) and classical swine fever virus (CSFV) (0.4%). Serology tests were conducted on 11,305 samplesand reported positive for Brucella suis (15.32%), Brucella abortus (0.62%), Brucella melitensis (0.85%), and melioidosis (0.05%). Parasitology analyses on 99 samples. revealed presence of 10.1% coccidia and 1% each of helminths and Sarcocystis. Within the 4-year period, there were no positive samples for porcine parvovirus (PPV), Nipah virus, swine influenza virus (SIV), and bacteria of Johne’s disease and leptospirosis. Continuous assessment is required to establish a comprehensive baseline data of swine diseases in Malaysia.

Increased emergence in microbial resistance to antibiotics is a growing problem globally. A retrospective investigation was made of antimicrobial resistance in a total of 2,345 Escherichia coli isolates from clinical cases submitted to Regional Veterinary Laboratory of Bukit Tengah, Penang, Malaysia between January 2000 and December 2015. Analysis using WHONET 5.6 revealed that resistance to ampicillin, norfloxacin and aminoglycosides was detected in isolates from poultry and pigs more often than ruminants. Resistant to colistin was higher in ruminants compared to other animal groups. Nor floxacin susceptibility was very high in isolates from ruminants, pet birds (82.2%) and zoo animals (88.6%). Isolates from pet birds had higher resistant rate to all of the antimicrobials tested compared to zoo and companion animals. Multidrug-resistant (MDR) E. coli isolates (29.25%, 684/2345) were detected in this study. Further efforts, for instance, national monitoring and surveillance, are required to quantify the exposure of animals to antimicrobial agents and resistant pathogenic or commensal bacteria in the animals and also its environment. Such useful information indeed will assist the prudent use of antimicrobial agents in veterinary practice; therefore, suppress the emergence of antimicrobial resistance in animals.

A total of 65 Pasteurella multocida were isolated and identified from various animal’s samples received by Veterinary Research Institute (VRI) during the period of 2014 to 2016. These animals comprises of cattle, goat, pig, chicken, duck and rabbit. The serogroup of Pasteurella multocida were carried out using designation system of Carter’s capsular typing and molecular serogrouping method. Based on cases submitted to VRI, the prevalence of pasteurellosis in Malaysia ranging from 1.0% to 3.2% (2014 to 2016). It is low compared to previous reports and the pattern of predominant serogroups and animal hosts were found to be changing every year. In 2014, 80% (12/15) of the isolates were Pasteurella multocida Carter’s type D where all were isolated from goats. In 2015, the predominant serogroup changed to Pasteurella multocida Carter’s type A with a prevalence rate of 40.6% (13/32) which were mostly isolated from duck and cattle. While for Pasteurella multocida Carter’s type D, the prevalence in 2015 reduced to 21.9% (7/32) compared to the previous year and it was isolated from various animal species. Interestingly, in 2015 there was one isolate of Pasteurella multocida Carter’s type B isolated from goat with no reported history of outbreak. In 2016, the prevalence of Pasteurella multocida Carter’s type A increased to 72.2% (13/18), with a high percentage (92.3%) infection in young calves showing clinical signs with high mortality and morbidity in infected farms. Furthermore, during these 3 years of study, 3 isolates of Pasteurella multocida serogroup F were also identified each from pig, goat and chicken, respectively. In conclusion, this study revealed that pasteurellosis had become sporadic in Malaysia and the distribution of serogroups were diverse in all species of animal with no definitive host.

Infection with gastrointestinal helminths has been a key factor affecting profitable animal production globally. Pig production in Nigeria has been adversely affected by presence of endoparasites. However, type of husbandry system can affect the prevalence of the parasites, thus, there is a need for this study to look at the prevalence of gastrointestinal parasites in the production of pigs in this tertiary institutional farm in Southwestern Nigeria. Faecal analysis for intestinal helminths was conducted on the pigs using saturated sodium chloride floatation technique. One hundred and fifty faecal samples were taken with only Ascaris suum, Oesophagostomum spp and Trichuris spp ova identified in single and mixed infection. The overall prevalence rate was 20%. Mixed infections remained more dominant (15.31%) with Ascaris suum having the highest occurrence as a single infection (3.33%), followed by Oesophagostomum spp (2%) and Trichuris spp (1.33%). Females had higher prevalence of nematode infection (14.67%) compared to males (5.33%). Comparing breeds, Large White had 14.67% prevalence of nematode infection which is higher than that of Duroc (5.33%). Weaners had no presence of helminths, whereas growers had the highest prevalence (9.33%), while piglets and adults had the same prevalence of nematode infection (5.33%). One of the factors that affect good productivity in pig farming is low presence of helminthes. Pork eaters are hereby encouraged to buy their meat from institutional farms as they produce safer meats for consumption.

PCV-2 associated disease and related losses occur commonly in high health status herds. With the advent of PCV-2 vaccines, these losses can be successfully reduced. In this study, there was a significant reduction (48%) in the mortality rate of the vaccinated pigs when
compared to the non-vaccinated pigs. Vaccination was conducted at day 21. In the vaccinated group, mortality was reduced from 5.25 % to 2.73%. This data indicates that the implementation of a PCV2 vaccination program can reduce the mortality rate of high health status pigs in Malaysia.

A trial and pig performance data collection was conducted to evaluate the efficiency of Luodong Bio- Fermentation Treatment Technology with zero discharge effect in a pig close house system at Tanjung Sepat, Selangor. This trial was done to gather and monitor the pig growth performance and the pig acceptance of using the Loudong Bio-fermentation Waste Treatment Technology in close house enviroment. Growth performance of the pigs were monitored by an average daily gain, feed conversion ratio, behaviour and veterinary observations. Close house temperature, moisture measurement and
bedding sampling were also conducted.

Melioidosis usually results in chronic debilities that reduce the
productivity of animals and condemnation of carcasses in abattoir. Melioidosis is reemerging among animals and humans, and anecdotal reports suggest an increase in disease observation. This study described the seroprevalence of melioidosis in livestock based on the data obtained from the Department of Veterinary Services, Putrajaya and the Veterinary Research Institute, Ipoh. The data were summarized according to animal species, state, and year. The seroprevalence rate in animals was 7.6, 48.2, 2.6, 13.6 and 3.6% in cattle, buffaloes, goats, sheep and pigs respectively. The
seroprevalence of the disease varies in different states of the federation. For all species, the seroprevalence vary between
2.6% and 48.2%. The seroprevalence over the years increased from 4.2% in 2000 to 12.0% in 2003 after which it varies between
the period 2004- 2007 and apparently declined between 2007 and 2009.