Уточнить поиск
Результаты 1-4 из 4
Immune-enhancing activity of potential probiotic strains of Lactobacillus plantarum in the common carp (Cyprinus carpio) fingerling
2018
Kazuń, Barbara | Małaczewska, Joanna | Kazuń, Krzysztof | Żylińska-Urban, Joanna | Siwicki, Andrzej K.
Introduction: Immune-potentiating functions of Lactobacillus plantarum strains in the common carp were evaluated. Material and Methods: Fourteen days of feeding fish dry diet supplemented with the bacteria provided parameters of nonspecific humoral immunity (lysozyme, ceruloplasmin, γ-globulin, total protein levels, and serum bactericidal activity) and cellular immunity (pinocytosis, respiratory burst activity, and potential killing activity of organ phagocytes), as well as the proliferative response of organ lymphocytes stimulated with mitogens. The resistance of fish to infection with Aeromonas hydrophila was also determined. Results: Dietary supplementation with L. plantarum had a substantial influence on the activity of organ phagocytes, especially the potential killing activity of head kidney cells. A significant increase in the proliferative activity of LPS-stimulated B lymphocytes and in the levels of γ-globulins and total protein was observed. The supplemented diet conveyed higher resistance than the control diet as the cumulative fish mortalities after infection with A. hydrophila were 65% and 85%, respectively. Conclusion: The results indicate that dietary supplementation with L. plantarum stimulates the antibacterial resistance of common carp and may reinforce defence against bacterial infections, but further studies need to be conducted.
Показать больше [+] Меньше [-]Effects of newly developed synbiotic and commercial probiotic products on the haematological indices, serum cytokines, acute phase proteins concentration, and serum immunoglobulins amount in sows and growing pigs – a pilot study
2018
Czyżewska-Dors, Ewelina | Kwit, Krzysztof | Stasiak, Ewelina | Rachubik, Jarosław | Śliżewska, Katarzyna | Pomorska-Mól, Małgorzata
The aim of the study was to determine the effects of supplementation of sows’ and growing pigs’ diets with three newly developed synbiotic and two extant commercial probiotic products on selected immune parameters under field conditions. The study was performed on 30 sows and 48 piglets of the Danbred breed. Immune parameters such as concentration and proportion of white blood cells and their subpopulations, immunoglobulins amount in serum, and serum concentration of cytokines and acute phase proteins were recorded with the use of a haematology analyser and ELISA kits. No significant differences between treatment groups and controls were found with regard to the immune parameters evaluated except for serum immunoglobulin concentration, which was significantly increased by synbiotic products B and C and probiotic product D. The results of the study indicate that the synbiotic products B and C and probiotic product D are worthy of further investigation as promising candidates to improve the immune status of healthy sows and their offspring.
Показать больше [+] Меньше [-]Effect of probiotics in fermented palm kernel meal (FPKM) and total mixed ration (TMR) to improve milk production in Mafriwal dairy cattle of Malaysia Veterinary Institute (IVM) farm
2018
Mohamad Noor I. | Norfadzrin F. | Haryani H. | Ahmad Malike H. | Sabariah B. | Saiful Adli A. H. | Aswanimiyuni A.
A preliminary study on the effect of probiotics in fermented palm kernel meal (FPKM) and total mixed ration (TMR) to improve milk production in dairy cattle was carried out. TMR feeding enhances feed intake, improves the ecology of the rumen leading to stimulated microbial activity to digest more feed, and then finally increases productivity of the cows. Probiotics ofBacillus spp. was given through concentrate after a fermentation process for three days. The trial was carried out at the Dairy Unit, Veterinary Institute, Kluang, Johore. Twenty Mafriwal cows with low milk production were selected and divided into twoequivalent groups. Each group consisted of 10 cows. Group A were fed with TMR. Group B received TMR and 100 grams perhead/day FPKM (FPKM, probiotics Bacillus spp. and mollases). The cows were given TMR daily which consisted of 20 kg per headof fresh Napier grass, 5 kg per head palm kernel meal, 1 kg per head soya bean meal, 1 kg per head corn, 0.5 kg per head molassesand 0.5 kg per head of enalac. Feeding trial period was carried out for four weeks. Feed was given twice a day, morning and lateafternoon, meanwhile water was given adlibitum. Animals were given the same diet for seven days for an adaptation period.Milk was sampled twice a day for four weeks and were recorded accordingly. The average of daily and weekly milk productionwere calculated. The animals from Group B showed an improvement of milk production before trial to the fourth week from 5.2litres/head/day to 6.99 litres/head/day i.e. 14.80% to 34.44% as compared to Group A.
Показать больше [+] Меньше [-]Effects of oral Akkermansia muciniphila supplementation in healthy dogs following antimicrobial administration
2018
Jugan, Maria C. | Rudinsky, Adam J. | Gordon, Alexander | Kramer, Denise L. | Daniels, Joshua B. | Paliy, Oleg | Boyaka, Prosper | Gilor, Chen
OBJECTIVE To measure effects of oral Akkermansia muciniphila administration on systemic markers of gastrointestinal permeability and epithelial damage following antimicrobial administration in dogs. ANIMALS 8 healthy adult dogs. PROCEDURES Dogs were randomly assigned to receive either A muciniphila (109 cells/kg; n = 4) or vehicle (PBS solution; 4) for 6 days following metronidazole administration (12.5 mg/kg, PO, q 12 h for 7 d). After a 20-day washout period, the same dogs received the alternate treatment. After another washout period, experiments were repeated with amoxicillin-clavulanate (13.5 mg/kg, PO, q 12 h) instead of metronidazole. Fecal consistency was scored, a quantitative real-time PCR assay for A muciniphila in feces was performed, and plasma concentrations of cytokeratin-18, lipopolysaccharide, and glucagon-like peptides were measured by ELISA before (T0) and after (T1) antimicrobial administration and after administration of A muciniphila or vehicle (T2). RESULTS A muciniphila was detected in feces in 7 of 8 dogs after A muciniphila treatment at T2 (3/4 experiments) but not at T0 or T1. After metronidazole administration, mean change in plasma cytokeratin-18 concentration from T1 to T2 was significantly lower with vehicle than with A muciniphila treatment (−0.27 vs 2.4 ng/mL). Mean cytokeratin-18 concentration was lower at T1 than at T0 with amoxicillin-clavulanate. No other significant biomarker concentration changes were detected. Probiotic administration was not associated with changes in fecal scores. No adverse effects were attributed to A muciniphila treatment. CONCLUSIONS AND CLINICAL RELEVANCE Detection of A muciniphila in feces suggested successful gastrointestinal transit following oral supplementation in dogs. Plasma cytokeratin-18 alterations suggested an effect on gastrointestinal epithelium. Further study is needed to investigate effects in dogs with naturally occurring gastrointestinal disease.
Показать больше [+] Меньше [-]