The in vitro and in vivo binding of a monoclonal antibody (MAB) that recognizes a tumor-associated carbohydrate antigen was studied in dogs. Monoclonal antibody 155H.7 was raised in response to inoculation of mice with beta-galactose(1-3)betaN-acetylgalactosamine conjugated to human serum albumin. Avidin-biotin-complex immunohistochemical staining of cryostat sections of normal and neoplastic canine tissue specimens revealed heterogenous binding of MAB 155H.7 to the cells of many canine mammary and lung carcinomas and homogenous staining of many sarcomas, including osteogenic sarcoma. In addition, there was variable staining of a variety of normal tissues including some ductual epithelium, peripheral nerve fibers, and some endothelial cells and fibroblasts. Immunoscintigraphy with 131I-labeled MAB 155H.7 was used to study the in vitro distribution of the antibody. The 131I-labeled MAB 155.H.7 was administered to 1 clinically normal dog, 7 dogs with osteogenic sarcoma, 1 dog with undifferentiated sarcoma, and 2 dogs with mammary tumor. Scintigraphy revealed concentration of radioactivity in 8 of 10 tumor sites within 24 hours after MAB administration. The ratio of 131I in tumor sites to 131I in the surrounding normal tissues, compared with the similar ratio of 99mTc-labeled erythrocytes ranged from 1.1 to 4.3 in tumor vs normal tissue with a mean value of 2, confirming tumor localization of the radiolabeled MAB in excess of that associated with enhanced tumor vascularization.

The activity concentrations of sup(40)K, sup(238)U, sup(232)Th and sup(137)Cs were measured using gamma spectrometric method in different food chain samples from Serb Belgrade environment during the periods May-June 2007 and May-June 2008 year. Relatively high activities of sup(40)K and sup(137)Cs were detected in the soil. These results indicate that sup(137)Cs is present in Belgrade environment even 20 years after nuclear accident in Chernobyl. However, in the samples of feedstuffs, animal products and bio indicators (meat of wild animals and fish), activity concentrations of primordial radionuclides and sup(137)Cs were low and below the detection limits. Results of these trials have shown that investigated animal products from the natural environment around Belgrade, are radioactivity safe.