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Immunogold studies on the gonadotropes in adenohypophysis of the Korean native goat
1999
Lee, I.S. | Lee, H.S. | Song, S.H. | Yoon, S.T. | Hwang, I.K. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine) | Seo, J.H. (Seoul National University, Seoul (Korea Republic). College of Medicine | Kang, T.C. | Won, M.H. (Hallym University, Chuncheon (Korea Republic). College of Medicine)
There have been a number of studies of gonadotropes secretin LH and FSH in the adenohypophysis, but the pattern of hormone storage and secretion of these cells still remains a controversial matter. In this study, we examined whether gonadotropes contained both of LH and FSH, and if so, how these hormones were distributed within the secretory granules. Hypophyseal sections of Korean native goat were simultaneously immunostained for LH and FSH antisera by protein A-gold technique. It was found that most gonadotropes contained both FSH and LH, but hormone storages in the secretory granules were some different among cells. Three types of gonadotropes were identified by the shape and size of the secretory granules and their hormone storage patterns. One type(I) of gonadotropes contained oval secretory granules, which immunoreactivity for FSH and LH were very weak. The size of secretory granules ranged from 160 to 310nm in diameter. Most granules contained both FSH and LH, but some contained only one of them. In another type(II)of gonadotropes, the immunreactivity and hormone storage patterns of the secretory granules were similar to those of type I cells. However, the secretory granules were round in shape and larger in size than those of type I. The other gonadotropes(type III) were distinctly distinguished by plenty of hormones in their secretory granules which were densely packed with numerous immunolabelled gold particles. These data are some inconsistent with other results that have been obtained in other ruminants like as cattle and sheep.
Показать больше [+] Меньше [-]Рубцовое пищеварение и продуктивность бычков при разном соотношении расщепляемого и нерасщепляемого протеина
2010
Kovalevskaya, Yu.Yu., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
In the conditions of the Republic of Belarus there was analyzed the influence of diets with different ratio of degradable and by-pass proteins on morphological and biochemical blood composition and productivity of bulls of black-and-white breed. In course of the study there was analyzed the average feeding diet of young stock of cattle in accordance with actually eaten fodders; morphological and biochemical blood indexes; live weight and average daily weight gain; results of a control slaughter. Research results showed the feeding at the finishing period of fattening-off with different ratios of degradable and by-pass proteins in ratio 60-65:40-35 made it possible to obtain 1049-1078 grams of live weight gain of bulls per day in the conditions of fodder expenses of 8,22-8,40 fodder units per 1 kilogram of weight gain. As a result the energy of weight gain was 20,1-21,2 mJ. Conversion of metabolizable energy at weight gain stage was at the level 20,4-21,8%. Expenses of metabolizable energy per 1 mJ at weight gain were 4,83-5,05 mJ, and prime cost of body weight gain lowered on 5% in comparison with control.
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