In the conditions of the Republic of Belarus there was realized a comparative study of peculiar features of influence of combined application of Khelavit preparation and protein-vitamin-mineral feed supplements Khendriks and Spaeta-kontsentrat on health status of cows and quality of obtained cow milk after application of these preparation for the preventive treatment of osteodystrophy. In course of study there were analyzed haematologic and biochemical blood indexes of cows after application of the experimental preparations. Results of physical and chemical indexes of milk were presented. Research results showed that the analyzed protein, vitamin and mineral feed supplements caused the normalization of biochemical blood indexes in comparison with animal of a control group. Besides, application of mentioned above drugs promoted the increasing of veterinary and sanitary quality and technological properties of produced milk.

In the conditions of the Republic of Belarus there was studied the efficiency of application of forage chemical preservatives Kormoplyus-1, Kormoplyus-2, and Kormoplyus-3 for grass fodder conservation. The analyzed preservatives contained acetous acid, urotropine and sodium acetate (only in Kormoplus-2). In course of the study there were established experimental batches with the studied sreservative; there was determined a chemical composition of prepared forages; in physiological and scientifical and economic experiment there was analyzed the feeding efficiency of cereal ensilage. Research results showed that application of preservatives Kormoplyus-1 and Kormoplyus-2 2 for preparation of cereal ensilage made it possible to receive a high quality ensilage and to increase the dry matter safety on 4,2-8,7% and protein on 12-9,4% on comparison with storage without preservatives. Also, it made it possible to increase the digestibility of dry matter in cattle diets respectively on 5.5% and3.7%; organic substance - on 5.5% and 4.0%; fibre - on 16,5% and 15,0%, and also to increase nitrogen deposition on 17,4 and 20,8%. Feeding with forages preserved with Kormoplyus-1 and 2 did not render negative influence on a health state and biochemical indices of animal blood.

In the conditions of the Republic of Belarus there was analyzed the efficiency of application of a probiotic lactic casein feed additive DKM which was developed in the Institute of Microbiology of the National Academy of Sciences of Belarus. The analyzed additive contained specially selected lactic-acid bacteria with a high bioartificial potential of metabolites. In course of the experiment there was studied the consumption of fodders (milk; oats; mixed fodders) by experimental calves; dynamics of live weight gain in the beginning and at the end of the experiment; average daily weight gain. Also, there were analyzed the background indexes of blood serum of calves, as well as the indexes of blood serum of calves in a month after application of DKM additive. Research results showed that application of the feed additive DKM promoted better palatability and use of fodders and rendered positive influence on animal productivity. Also, there was marked the normalization of metabolism that was proved y the laboratory tests of blood serum.

Investigation of connection of experimental trichophytosis and relations between changes of clinical implications of disease and dimensions of some immunological indexes was realized in the conditions of the Republic of Belarus. At the first stage of the experiment there analyzed 48 guinea pigs (Cavia porcellus) infected with Trichopyton verrucosum. Phagocytic ability of phagocytes and level of circulating antibodies in indirect hemagglutination test were investigated each 5 days before and during disease. The second stage was realized by the example of 2,5-6 months old calves. In course of study there were analyzed blood and blood serum indexes, indexes of nonspecific resistance, as well as some indexes of cell-mediated immune response and antibody mediated immunity. Comparative analysis the following morphological and biochemical blood indexes of clinically healthy calves, calves after trichophytosis, and calves sick with mild, moderate and severe of disease were presented: erythrocytes; hemoglobin; leukocytes; basophils; eosinophiles; rods; microxyphils; lymphocytes; monocytes; ESR; total albumin; albumins; alpha globulins; beta globulins; gamma globulins; carotene; calcium; inorganic phosphorous; alkaline reserve; copper. The realized experiment made it possible to conclude that determination intensity of leukocyte absorption of microconidia of the agent, antibodies against the agent, and specific for cytoplasm fungus, morphological and biochemical blood indexes made it possible to control the development of inflammatory phenomenon in centers of infection, as well as to predict the formation of face, infiltrative and suppurant disease forms

Investigation of vitamin supply of cattle affected by fascioles in association with strongylatosis of gastro-intestinal tract was realized in the conditions of the Republic of Belarus by an example of white-and-black 3-8 year old cows. Analysis of blood and blood serum of experimental animals showed that content of B1, B2, and E vitamins was lower in group of animals sick with fascioliasis and strongylatosis of gastro-intestinal tract. There were stated no changes in A and C vitamins content. Content of pyroracemic acid exceeded control indexes and was 22,0 +/- 0,173 mg/l. Content of vitamin A did not change and was 0,011 +/- 0,0012 mg/l for sick animals and 0,013 +/- 0,0043 mg/l at healthy animals. Content of vitamin C was different for sick and healthy animals: 1,685 +/- 0,190 mg/l and 1,93 +/- 0,294 mg/l, respectively. Research results proved that treatment of cattle sick with helminth infestation should be realized in complex. It was proposed to apply premixes and vitamin preparations containing B1, B2, as well as A, E, and C vitamins for the increasing of natural protection and for the creation of unfavourable conditions for disease agents

On the basis of the data analysis of morphological and biochemical blood traits it was determined that blood indices of all groups of pigs didn't break the limits of physiological norms. Pigs of Canadian selection differ by high metabolism intensity and immunity of organism

Studying of influence of various levels of selenium on growth, protein structure of blood serum, natural resistance, and reproductive performance of pedigree bull-calves was realized in the conditions of the Republic of Belarus. It was established, that feeding with an optimum dose of selenium (0,4 mg per 1 kg of dry matter) in rations of replacement bull-calves during the raising period promoted the increasing of daily average weight gain on 6,8%, indexes of natural resistance - on 0,5-13,9%, and sperm production quality - on 4,5-27,3%. In the conditions of optimization of selenium level of in diets the protein structure of blood serum of replacement bull-calves exceeded the similar index at control animals by the crude protein index - on 8,3 (Р less than 0,05), by albumine and alpha globulin -on 2,2% and 1,6%, respectively. Application of selenium in dose of 0,4 mg per 1 kg of dry matter made it possible to increase the indexes of natural resistance on 0,5-13,9%. Increase of selenium dose up to 0,4 mg per 1 kg of dry matter in made it possible to increase the quantity and quality of sperm of bull-calves, that was proved by the increasing of ejaculate volume on 4,5%, concentration of male germ cells in ejaculate – on 15,7% (Р less than 0,05) and male germ cells activity - on 7,3% (Р less than 0,001)

Due to Herbal medicine it is possible to use cheap and ecological clean preparation. Studied medicinal forms of wormwood bitter (the wormwood bitter extract, fluid extract, Artemizitan) in therapeutic dose stimulate the factors of natural resistance beside animals. Results of studying of lysozyme activity in blood serum have shown that preparations of wormwood bitter have caused authentic increase of this indicator in comparison with control animals, both in pigs, and at sheep. Bactericidal activity of blood serum was authentically more than in control group, both at pigs, and at sheep after introduction of infusion of wormwood bitter. Studying of influence of wormwood bitter on phagocytosis indicators has shown, that all medicinal forms of plant have made stimulating impact on phagocytal activity of neutrophils and phagocytal number both in experiences on pigs, and on sheep. Artemitizan has caused authentic increase and phagocytal index. Thus, the research will be coordinated with literary data, that preparations of wormwood bitter make stimulating impact on indicators of natural resistance of animal blood | Благодаря фитотерапии возможно использование дешевых и экологически чистых препаратов. Изученные лекарственные формы полыни горькой (настой полыни горькой, жидкий экстракт полыни горькой, артемизитан) в терапевтических дозах стимулируют показатели естественной резистентности у животных. Результаты изучения лизоцимной активности в сыворотке крови показали, что препараты полыни горькой вызвали достоверное повышение этого показателя по сравнению с контрольными животными, как у свиней, так и у овец. Бактерицидная активность сыворотки крови была достоверно больше, чем в контрольной группе, как у свиней, так и у овец после введения настоя полыни горькой. Изучение влияния полыни горькой на показатели фагоцитоза показало, что все лекарственные формы растения оказали стимулирующее влияние на фагоцитарную активность нейтрофилов и фагоцитарное число как в опытах на свиньях, так и на овцах. Артемизитан вызвал достоверное повышение и фагоцитарного индекса. Таким образом, наши исследования согласуются с литературными данными, что препараты полыни горькой оказывают стимулирующее влияние на показатели естественной резистентности крови животных

There was studied the feature of hematological and biochemical blood indicators of rats at action of acute ionising radiation against acute stress influences. Results of research of hematological and biochemical blood indicators of rats developing under influence of stress and radiating factors are presented. It is established, that on 4 days twenty-four-hour period after the influence of pointed ionizing radiation against a background of pointed stress impact some changes become evident. They appear in reducing of total number of leucocytes, haemoglobin, hematocrit and general albumen, and also in increase of such ferment activity as lactate dehydrogenase, creatine phosphokinase, aspartate aminotransferase, alanine aminotransferase. | Целью исследования является изучение особенности гематологических и биохимических показателей крови крыс при действии острого ионизирующего излучения на фоне острого стрессорного воздействия. Представлены результаты исследования гематологических и биохимических показателей крови крыс, развивающихся под влиянием стрессорного и радиационного факторов. Установлено, что на 4 сутки после действия острого ионизирующего излучения на фоне острого стрессорного воздействия отмечаются изменения, выражающиеся в снижении общего количества лейкоцитов, гемоглобина, гематокрита и общего белка, а также увеличении активности ферментов лактатдегидрогеназы, креатинфосфокиназы, аспартатаминотрансферазы и аланинаминотрансферазы.

There was studied the activity of alaninaminotransferase and aspartataminotransferase, alkaline phosphatase, gamma-glutamiltranspeptidase, lactate dehydrogenase, cholinesterase, alpha amylase in blood serum, liver and pancreas of replacement young hens. At carrying out of immunization of replacement young hens by a vaccine against infectious laryngotracheitis, developed in Institute of experimental veterinary science, reaction from the party not only bodies of immune system, but also such bodies, as liver and pancreas, takes place. Biochemical reaction of the specified bodies to immunization is a consequence of adaptability of an organism to vaccinal stress. Biochemical changes of display enzymes in blood serum, pancreas and liver are found out. It was marked increase of activity of the given enzymes in liver, pancreas and blood serum so, activity of alaninaminotransferase and aspartataminotransferase, alkaline phosphatase, gamma-glutamiltranspeptidase, lactate dehydrogenase increased at birds of trial group in 1,3-1,9 times in comparison with the control. In the tabular form data on activity of alaninaminotransferase, aspartataminotransferase, alkaline phosphatase in liver, pancreas and blood serum of repair young growth of the hens vaccinated against infectious laryngotracheitis are presented. | Целью наших исследований явилось изучение активности аланин- и аспартатаминотрансфераз, щелочной фосфатазы, гамма-глутамилтрансферазы. лактатдегидрогеназы, холинэстеразы, альфа-амилазы, в сыворотке крови, печени и поджелудочной железе ремонтного молодняка кур. При проведении иммунизации ремонтного молодняка кур вакциной против инфекционного ларинготрахеита, разработанной в Институте экспериментальной ветеринарии им. С.Н. Вышелесского НАН РБ имеет место реакция со стороны не только органов иммунной системы, но и таких органов, как печень и поджелудочная железа. Биохимическая реакция указанных органов на иммунизацию является следствием адаптивности организма к вакцинному стрессу. Выяснены биохимические изменения индикаторных ферментов в сыворотке крови, поджелудочной железе и печени. Отмечалось сочетанное повышение активности данных ферментов в печени, поджелудочной железе и сыворотке крови, так, активность АлТ, АсТ, ЩФ, ЛДГ, ГГТФ повышалась у птиц опытной группы в 1,3-1,9 раза по сравнению с контролем. В табличной форме представлены данные по активности аланинаминотрансферазы (АлТ), аспартатаминотрансферазы (АсТ), щелочной фосфатазы (ЩФ) в печени, поджелудочной железе и сыворотке крови ремонтного молодняка кур, вакцинированных против ИЛТ