Drug interaction is the effect produced by combination of various drugs administered in a biological system which may produce beneficial or adverse effects.Due to continuous usage of antibiotics to bacterial infections, pathogenic bacteria developed resistance mechanisms which leads to reduced antibacterial activity of different antibiotics. Combination of antibiotics with phytochemicals became an alternative to enhance the efficacy of antibiotics. In this connection,in the present study, the effect of certain phytochemicals namely Quercitin, Morin, Ellagic acid,  Chloragenic acid Rutin and Naringenin in combination with antibiotics Amoxycillin and Ciprofloxacin were evaluated. Staphylococcus aures and Escherichia coli were used as test organisms. They were isolated from the mastitis milk samples and their presence was confirmed using Polymerse Chain Reaction .The antibacterial activity of the antibiotics and antibiotics in combination with phytochemicals were determined using broth dilution method and agar well diffusion assay. The MIC and zones of inhibition were used as indicators for drug interaction.