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Detection of Y chromosome of bovine using testis specific protein and amelogenin genes
2016
Mohd Hafizal A. | Mohd Hafiz A. R. | Nor Aini W. | Suriaty R. | Halimaton Sa’adiah T. | Nurizan A.
A total of thirty-eight Mafriwal cattle were selected from a localcattle herd of a government cattle farm; of which 36 animals were sub-fertile Mafriwal female dams and two bulls which were considered as control animals (one male Mafriwal and one male Jersey). Two markers were used in the detection of Y chromosome in the sub-fertile female animal which are testis specific proteins Y-encoded (TSPY) and amelogenin (AMLX/AMLY) genes. The genes were amplified using PCR. The DNA bands from a normal male for TSPY gene size was approximately 260 bp while AMLX/ AMLY gene were approximately 341 and 467 bp. The examination of all samples showed that the sub-fertile cow revealedonly 467 bp while three fragments were detected in the control group; 260 bp (testis specific protein, Y-encoded gene), 341 and 467 bp (Amelogenin gene). The results showed that the sex chromosomeanomalies associated with Y chromosome did not occur in this group. These two sex markers can be used for the diagnosis of Y chromosome abnormality in a sub-fertile cow through polymerase chain reactionwhich is a rapid and reliable method for use in breeding herds.
Показать больше [+] Меньше [-]Surgical management of unilateral seminoma in a dog
2015
John Martin, K. D. | Joy, Bini | Narayanan, M. K.
Seminomas are one of the common testicular tumours that occur mostly in dogs above five years of age. A case of nine-year-old male German Shepherd dog, presented to University Veterinary Hospital, Kokkalai with a history of swelling on the scrotum for the past two months and its successful surgical management is discussed. The right testicle was found to be highly enlarged and hard while the other atrophied. Histopathological examination following its surgical removal, confirmed it as seminoma.
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