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Production and partial purification of Staphylococcus aureus alpha toxin
1999
Park, H.M. | Oh, T.H. | Han, H.R. (Seoul National University, Suwon (Korea Republic). Department of Veterinary Internal Medicine, College of Veterinary Medicine)
Alpha toxin of S aureus has cytolytic activity respectively. This antigen has been received the most attention since it is a major virulence factor in pathogenesis of staphylococcal mastitis. Thus, alpha toxin has been focused as potential candidate ofvaccine tominimize mastitis in cows. the purpose of this study was to develop a simple, efficient production and purification methods of sufficient amount of alpha toxin antigen from S aureus. Alpha toxin production measured by hemolytic activity was the highest at 18 hrs postinoculation in yeast extract culture medium supplemented with thiamine, nicotinic acid and casamino acid. Alpha toxin was purifed by ammonium sulfate precipitation (65%) and ultrafiltration. Molecular weight of the toxin was 33 kDa in the analysis with SDS-PAGE. Conclusionally, when alpha toxin was included in the vaccine, the optimal harvest time of alpha toxin was at 18 hrs after inoculation in yeast extract medium supplemented with thiamine and nicotinic acid.
Показать больше [+] Меньше [-]Localization of adrenergic receptors in bovine esophageal groove
1993
Kang, T.M. | Cho, J.Y. | Yang, I.S. (Seoul National University, Seoul (Korea Republic). College of Veterinary Medicine) | Park, J.H. (Cheju National University, Cheju (Korea Republic). College of Agriculture)
Shiga-like toxin-2-producing Escherichia coli 0157 : H7 infection in gnotobiotic piglets : protection against brain vascular lesions with SLT-2 antiserum
1993
Chae, C.H. | Moxley, R.A. (Nebraska University, Lincoln (USA). Veterinary Diagnostic Center)
Shiga toxin, shiga-like toxin and verotoxin
1993
Chae, C.H. (Nebraska-Lincoln University, Nebraska (USA). Veterinary Diagnostic Center)
Studies on the Clostridium perfringens type C infection of pig in Korea
1993
Yeh, J.K. | Park, K.Y. (Bayer Veterinary Medical Research Institute, Seoul (Korea Republic)) | Cho, S.K. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
Rapid identification and toxin type analysis of Clostridium perfringens isolated from healthy or diseased stocks with necrotic enteritis in chicken
1997
Kim, H.J. | Chung, U.I. (Miwon Institute of Animal Science, Ichon (Korea Republic)) | Kang, M.I. (Chonnan National University, Kwangju (Korea Republic). College of Veterinary Medicine)
