Isolation and identification of mycoplasma were performed to clarify mycoplasma infection of mice fed by convectional feeding at two (K1, K2) institutes in Korea. The twenty mice to be tested were randomly samples from each of 10 breeding colonies in respective institute. Identification of the mycoplasma strains isolated from the nasal cavity, lung and synovia of mice was made according to the morphology of colonies, biological and biochemical properties with special reference to M. pulmonis, M. arthritidis and M. neurolyticum

This paper deals with the incidence of antibiotic resistance and of R plasmids in 319 Salmonella strains isolated from various samples on 7 piggeries in Taegu, Kyongbuk, Kyongnam and Chungnam and a slaughter house in Taegu during the period from May, 1984 to May, 1985. Of the 319 strains examined, 250 (78.4%) were resistant to one or more antibiotics such as ampicillin (Am), chloramphenicol (Cm), gentamicin (Gm), kanamycin (Km), sulfadimethoxine (Su), streptomycin (Sm), rifampicin (Rf) and tetracycline (Tc), and higher resistant to Su (74.9%), Sm (53.0%) and Tc (28.5%). Only two Salmonella strains isolated from A and C farms were resistant to Rf and Gm, respectively

The measurement of angled erythrocyte sedimentation rate (ESR), as a replacement for perpendicular ESR, for cattle blood was scrutinized since it has been well known that perpendicular ESR in cattle was tpp slow to be adopted as an effective clinical test. Samples of blood were taken from 186 Korean native cattle over 2 years old. Average values of perpendicular ESR/24hrs in 15 apparently healthy cattle, as measured by Wintrobe, Westergren and capillary tubes, were 5.8 +- 2.2, 11.1 +- 3.7 and 10.4 +-4.5% respectively, which were found to be similar to the values of perpendicular ESR/hr of normal blood of human

Microcarrier culture technics were widely used for the massive production of the vertebrate animal cells. In this study, attempt was made to establish the microcarrier cell culture system using Cytodex. Various factors affecting the growth of cells on microcarrier culture were also discussed. In conclusion, the yield of cells in microcarrier culture was several times greater than those in roller bottle and flask culture methods, based on the volume of culture media

Sprague-Dawley rats received subcutaneous injections of gentamicin sulfate, 50 and 100mg/kg for 3, 7 and 10 days. The hematological and blood chemical values were determined. Kidneys were examined histologically and ultrastructurally. The serum values of aspartate transaminase, blood urea nitrogen and creatinine in the rats administered gentamicin, 50 and 100mg/kg/day were significantly increased than those in the control. The ratio of kidney weight to body weight was significantly increased in the rats injected gentamicin, 100mg/kg for 10 days than those in the control

In order to know the comparative histological structure of the tongue of some domestic and laboratory animals, the root of the tongue has been investigated by light microscopy. The filiform papillae were mainly distributed in the root of the tongue of most animals and many foliate papillae were also found in the dog and rabbit. The very well-developed mucosa was found in the cattle and in the dog, fowl, rat and mouse was fair. The lamina propria was well developed in the cattle, dog and pig. The muscle layer was well developed in the cattle. The adipose tissue which distributed between the muscle fibers was mainly found in the dog

In order to evaluate the effects of the cell-mediated immunity of the animal in the chronic diseases the guinea pigs and rabbits were inoculated with Mycobacterium bovis. After 6 weeks these animals were sensitized and challenged with 2, 4-dinitrochlorobenzene. The cutaneous reactions observed in these animal species were similar each other. Macroscopic and microscopic responses in the animal experimentally infected with M. bovis were markedly reduced compared to those in the control animals

The present study was carried out to observe the histopathological changes in the lung of A/J, C57BL/6N, DBA/2 and NIH-GP mice intratracheally instillated with benzo(a)pyrene or urethan. In the group of intratracheal instillation with benzo(a)pyrene and charoal powder, squamous cell carcinomas and adenocarcinomas were induced. Tumors were induced in high incidence in the lung of A/J and C57BL/6N mice. Especially, squamous cell carcinomas were induced in high incidence and were well-differentiated in the lung of A/J mice