Coagulase negative Staphylococci were considered as commensal organisms or contaminants with limited pathogenic potential. However, they are becoming more frequently associated with nosocomial infections in human and skin infections in animals. In this study, we attempted to isolate and identify the Staphylococci species that causes dermatological infections in dogs. Out of 35 isolates, 27 were identified as S.pseudintermedius and 3 as S.schleiferisubspschleiferi. Among three S.schleiferi isolates, 2 were closely related to S.schleiferi ATCC strain in pta gene sequences.  Methicillin resistant gene (mecA) was not detected in these isolates. All three isolates were susceptible to oxacillin, amikacin, gentamicin and tetracycline, intermediate susceptibility to ceftriaxone and resistant to Penicillin, ampicillin, sulphadiazine. S.schleiferi is an emerging cause of infections in veterinary patients. None of the isolate produced biofilm in microtitre tissue culture plate adherence method. This is the first report of isolation and identification of S.schleiferi from canine skin infections from India. Further studies are needed for early detection and virulence factors of S.schleiferi in the pathogenesis of pyoderma and otitis in dogs.

This experiment was carried out to study the effect of frozen storage on the physico-chemical quality of quail breast meat. Six weeks old broiler quails were collected, slaughtered, dressed hygienically. Quail breast meat samples were collected from dressed carcass which was packed in low density polyethylene pouches taking optimum care to avoid contamination and stored at -18 ± 20 C for 60 days.  Fresh as well as frozen quail breast meat samples were evaluated for physico-chemical and histological changes. Frozen meat samples were analyzed after thawing at 4 ± 10 C for 12 hrs at an interval of 15, 30, 45 and 60 days. It was observed that with advancement of the frozen storage period pH, TBA value, tyrosine value and drip loss increased, whereas moisture, protein, fat and ERV content of quail meat decreased. Pronounced structural changes reported at later part of storage in histological study due to formation of ice crystals in the muscles. It shows that quail meat can be safely stored for 60 days in frozen state without any marked changes.

The data on 1721 calves from Sahiwal and Jersey-Sahiwal crossbred cattle were utilized for studying the age specific mortality and culling rate. The overall mortality and culling rate were found to be 15.92 and 19.52 per cent in Sahiwal and Jersey crossbreds respectively. The average female calf mortality from 0-1, 1-3, 3-6, 6-12 and 12 month to age at first calving was observed as 5.81, 2.92, 2.06, 1.75 and 5.01 per cent, respectively. The genetic group had no significant effect on female calves mortality. The maximum culling rate was observed from 12 month to AFC.

The use of metallic silver as an antimicrobial agent has been recognized for centuries. Silver nanoparticles (SNP) are now incorporated in apparel, wound dressings, appliances, cosmetics, paints and plastics for their antimicrobial properties. Generally, silver nanoparticles are prepared by a variety of chemical methods. In this study, we have used Phyllanthus amarus and Andrographis paniculata herbal plants leaves and stem extracts to synthesis SNP and were characterized by UV-Vis spectroscopy and Transmission Electron Microscopy (TEM). SNP capped with plant extracts gave absorption peak at 420 nm as expected for silver and broadening of peak were also noticed. TEM images suggested that they were of almost spherical shape and in the range of 7-60 nm in size. Antibacterial activity of plant extracts capped SNP were tested in Mueller-Hinton agar by well diffusion method against Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, Escherichia coli and Salmonella Typhimurium.  SNP of 1 mg/ml and 5 mg/ml of P.amarus exhibited almost similar antibacterial activity, whereas, 5mg/ml, not 1 mg/ml of SNP capped with A. paniculata exerted antibacterial activity. The highest zone of inhibition was noticed against B.subtilis followed by S.aureus and P.vulgaris and the least zone of inhibition was observed against E.coli and S.Typhimurium. Green synthesis of SNP is cost effective and environment friendly. Further studies are required to explore the possibility of use of P.amarus capped silver nanoparticles for the treatment of burn and wound.

The present study was carried out to find out the histopathological alterations in gill, liver and kidney of Goldfish (Carassius auratus) under sublethal concentrations of Zinc. Fishes were exposed to sub-lethal concentrations of 0.572, 1.145, 2.289, 4.578 and 9.156 mg.l -1 for 28 days which are 1/10 of their 96hr LC. The histological samples of gill, liver and kidney were collected from 14 th, 50th  and 28th day and stained by haematoxylin and eosin, observed under trinocular microscope. Vacuolation, hyperplasia, epithelial lifting and lamellar fusion, shrinkage of blood vessels, secondary lamellar damage, breakdown of epithelial cells, blood congestion and necrosis were observed in gill. Rupture of nucleus, congested blood vessels, hemorrhage in liver sinuses, increased size of kupffer cell, increased pycnotic nucleus, hypertrophied hepatocytes, vacuolation, cellular necrosis, rupture of hepatocytes were observed in liver of zinc treated fish. Shrunken glomerulus, vacuolation, blood congestion, increased in space of periglomerular, increased in space of peritubular, melanomacrophages, loss of cytoplasm, fat deposition, degenerated tubules and degenerated glomerulus were seen in kidney. The above results showed that sub-lethal toxicity of Zinc alter the internal organs of goldfish at lower concentration.

Six dogs with unstable diaphyseal fracture of femur of different breeds of either sex and bodyweight ranging from 10 to 30 kg presented to the Small Animal Orthopedic Unit of the Madras Veterinary College Teaching Hospital, over a period of two years underwent fracture fixation with latest technique of linear locking compression plates. Different sizes and length of new generation locking plate system were used for different configuration of unstable fracture of femur. The post operative assessment of fracture healing was evaluated by radiographs at different intervals and estimation of bone specific serum alkaline phosphatase enzyme by fluorimetric methods. In the present study, the locking compression plating technique provided adequate apposition, stable fixation and promoted early weight bearing of traumatized limb. The locking plate system had a unique combi hole design in a single implant and enabled to select the function best suited for the fracture configuration to achieve the most stable fixation. The locking plate system acted as a single beam construct which increased the stiffness of the implant and was found appropriate in management of unstable or comminuted diaphyseal fracture of femur in dogs.

Restraining gadget for Indian poisonous snakes like Indian cobra and Russell’s Viper were designed, using available infrastructures. Tongs, collapsible tubes and bags were designed and applied in the poisonous snakes in order to have better restraining activities. These snakes reared at Guindy Snake Park Trust were successfully restrained, using the gadgets developed. However these gadgets were found not suitable for restraining procedure associated with Saw scaled viper and Common krait. The gadgets developed were useful for zoo veterinarians for control of these serpentines.

The carrot tops had high crude protein (144 g/kg), calcium (24.3 g/kg) and phosphorus (7.7 g/kg), comparable to sun hemp (Crotalaria junctea), fodder berseem and oil cakes, respectively. Milk from animals fed with carrot tops were free from residues of organochlorine, carbamate and organophosphorus pesticides. Carrot tops are perishable in nature and hence were preserved as feed blocks to extend shelf life. The nutritional value and keeping quality of the blocks were also studied. The studies revealed that carrot whole tops can be a promising alternate source of green fodder, especially in hilly regions where there is a shortage of green fodder during winter.

This study investigated oxyclozanide, a salicylanilide anthelmintic, as an alternative treatment for Methicillin-resistant Staphylococcus aureus (MRSA) in bovine mastitis. With conventional treatments facing challenges due to antibiotic resistance, oxyclozanide demonstrated promising in vitro antimicrobial effect against MRSA isolates. The minimal inhibitory concentration (MIC) ranged from 0.5 to 1.0 μg/ml, indicating its efficacy. Oxyclozanide’s mechanism involved disrupting the transmembrane structure of bacteria, presenting a potential strategy against MRSA. This suggested the repurposing of oxyclozanide as a viable option for combating MRSA in bovine mastitis, prompting further investigation into its precise mechanism and therapeutic potential.

The main aim of this study was to investigate the effect of different concentrations of glutathione supplementation on liquid storage of Surti buffalo bull semen. This study was performed on adult Surti buffalo bull (n = 6), and seminal ejaculates (24) were collected and evaluated for various microscopic seminal quality parameters for further processing. After preliminary evaluation, ejaculates of each collection session were mixed and divided into four equal aliquots. All the aliquots were diluted (1:10) with Tris fructose egg yolk citrate extender contained glutathione served as control (T0), whereas the other three aliquots were supplemented with 0.5, 2.0 and 5.0 mM glutathione which were grouped as Treatment-1 (T1), Treatment-2 (T2) and Treatment-3 (T3), respectively. Thereafter, the samples were stored at 4 ºC for 4 h, and various seminal parameters (individual sperm progressive motility, viability, abnormalities, plasma membrane functionality) were evaluated at equilibration. The results indicated that the mean percent values for pre-freeze sperm progressive motility, live sperm percentage and HOS responsive spermatozoa were found to be significantly higher (P<0.05) (except individual progressive motility which was non-significantly higher in Treatment-3); whereas sperm abnormalities were significantly lower (P<0.05) in semen samples treated with glutathione (Treatment-1, Treatment-2 and Treatment-3) in comparison to control. The Treatment-2 (2.0 mM glutathione), had the highest pre-freeze sperm progressive motility percentage, live sperm percentage, HOS response sperm percentage and reduced sperm abnormalities percentage compared to other three groups.