Fasciolosis is a disease caused by fluke infestation Fasciola gigantica and Fasciola hepatica. Usually, these flukes infect ruminants such as cattle, goats, sheep and wildlife. Infestation F. gigantica can cause a decrease in the immune response, so that livestock become more susceptible to infection with other microorganisms. The emergence of microorganisms such as bacteria can cause foodborne disease for those who consume liver infestation by F. gigantica. This study aims to see the presence of pathogenic bacteria in the liver of cattle infestation by F. gigantica. Isolation of bacteria present in beef liver was conducted by culturing it on culture media such as blood agar media. Blood agar media can be used to determine pathogenic bacteria. Pathogenic properties of bacteria are seen based on the ability of bacteria to hemolyze blood on blood agar media. Based on the results of the hemolysis test on blood agar media, four colonies were found that were potentially pathogenic, namely alpha hemolysis (isolate S2-A) and beta hemolysis (isolate S1.1-A, S2-B, and S3-A). Furthermore, the results of bacterial identification were based on morphological observations and Gram staining tests as well as biochemical tests and spore staining, isolate S2-A identified as bacteria Staphylococcus sp., while isolates S1.1-A, S2-B, and S3-A were identified as bacteria Bacillus sp. Thus, it can be concluded that in the liver of cattle infestation by F. gigantica, bacteria are found that have the potential to be opportunistic pathogens.

Parasites pose a major threat to animal health and welfare globally, with a greater impact in Africa, particularly Ethiopia, due to diverse agro-ecological factors that support various hosts and helminths. This experiment aimed to estimate parasite infestation levels, identify associated risk factors, and detect anthelmintic drug resistance. Fecal sedimentation and flotation techniques were used to identify trematode and nematode eggs, respectively. Two drugs, Albenda-qk 600 mg and Flukash Sheep (Oxyclozanide 340 mg), were tested for resistance, and associated risk factors were analyzed using STATA (version 13). Results showed nematode and trematode prevalence of 66% and 29% pre-treatment, dropping to 7% and 3% post-treatment. Multivariable logistic regression revealed that age and deworming timing were significantly associated with parasite positivity (P 0.05). Despite significant reductions in parasite prevalence post-treatment, some sheep flocks exhibited resistance. To prevent further development of anthelmintic resistance, the following practices are recommended: avoid frequent or unnecessary treatments, apply strategic and integrated drug use, ensure proper dosages, rotate anthelmintic drugs, and regularly perform efficacy tests.

Maggots are one of the fly larvae with a high protein content of up to 30-45%, so maggots can be used as an alternative animal feed ingredient. As one of the sources of animal feed raw materials, insects-based feed must also be safe from contaminants of microorganisms, including pathogenic bacteria. Therefore, this study aims to isolate and determine the screening of pathogenic and non-pathogenic bacteria from maggots' digestive systems. Maggots are cultivated on media based on animal faecal and organic waste for 2 months. Then, the bacteria are isolated and purified using nutrient agar media, and hemolysis tests are carried out on blood agar media and Gram staining. Eighty-seven bacterial colonies with various morphological characteristics were successfully isolated from the maggot digestive system. Most isolated bacteria are classified as Gram-negative bacteria with a bacilli form. Based on the results of the hemolysis test, as many as 16% of bacterial isolates are indicated by pathogenic bacteria because of their ability to hemolyze blood. However, only about 2% showed b-hemolysis. Thus, it can be concluded that the screening results of non-pathogenic bacteria are still more numerous compared to pathogenic bacteria present in the maggot digestive system.

Dadih is a traditional food product that is produced by storing buffalo milk in bamboo tubes and left for 2-3 days. During the process of making Dadih, milk ferments into Dadih in a bamboo tube. The aim of this research is to determine the quality of buffalo Dadih from pH values, acidity degrees, and total bacterial colonies with different storage durations and temperatures. This research used 12 bamboo tubes with two treatments and three repetitions. This research used a laboratory experimental method with a 2x3 factorial pattern. This research showed that the highest room and refrigerator pH values were found in the first week, with an average of 7.13 0.15 and 7.9 0.1. The highest acidity degree values from room temperature and refrigerator were in the third week, with 359.47 16.95 and 254.03 6.34. Another finding was that the highest total bacterial colonies at room temperature and in the refrigerator were in the third week, with 3.33 0.89 and 2.8 0.76. It can be concluded that the buffalo Dadih from Gayo has a pH value and a total value of bacterial colonies that meet SNI standards. Meanwhile, the total titrated acid in Dadih does not yet have an SNI standard.

Diabetes mellitus (DM) is a metabolic disorder and endocrinopathy characterized by hyperglycemia or a persistent increase in blood glucose levels. This case describes a male domestic cat exhibiting symptoms of vomiting, loss of appetite, and yellowish discoloration of the ears and oral mucosa, which was diagnosed with DM and hepatic-biliary disorders based on serum biochemistry and ultrasonographic examinations. The treatment included insulin injections, antibiotics, antiemetics, a liver supplement, and a specialized diabetic diet. After 14 days of insulin injection and a diabetic diet, the cats blood glucose levels returned to normal. A diabetic diet will be provided for the cat throughout its lifetime.

Mahyaveh, a traditional fermented fish product consumed as condiments in the southern part of Iran. In this study, the effects of the Lactobacillus plantarum and Lactobacillus  as starters culture on the chemical and microbial properties of fermented sauce were studied during a 45-days interval. The studied parameters were pH, acidity, crude protein, TVB-N, salt content, biogenic amines (i.e. Histamine and tyramine), halophilic bacteria, LAB, mold and yeast counts. The results showed that pH values and crude protein content were significantly different among the studied groups (p<0.05). The interaction between time and applying starters on significant changes in acidity was observed. The highest amount of TVB-N was measured for the treated group. The amount of histamine and the salt content of the samples decreased over time. There was also a significant difference in the tyramine levels of the samples (p<0.05). The highest count of Halophiles, Bacillus, Mold and Yeast were observed in the control group. The cfu of LAB increased during the time of fermentation process. It can be concluded that the inoculated sauce with L. plantarum and L.starters was more acceptable in terms of chemical and microbial properties compared to the control group.

A gravid, previously-injured, female, wild Eastern chicken turtle (Deirochelys reticularia reticularia) was treated by the North Carolina State University College of Veterinary Medicine Turtle Rescue Team for prolonged anorexia and possible dystocia.  Medical management included successful induction of oviposition with oxytocin.  Radiographs and ultrasonography were used to confirm the presence of eggs within the oviducts prior to oxytocin therapy.  Ultrasonography revealed Doppler blood flow within at least one egg and the presence of a developing embryo.  It is hypothesized that these findings may be unique to Eastern chicken turtles.  This case demonstrated that case management may be complicated by a patient’s medical history and also unique life-history traits.

Yersiniosis, caused by Yersinia ruckeri, is recognized as one of the most significant diseases affecting cold-water and sturgeon fish in aquaculture. Vaccination has emerged as the most cost-effective and sustainable method for controlling infectious diseases in fish. This study investigated the effects of a bath vaccine against yersiniosis on certain biochemical parameters in the serum of beluga sturgeon (Huso huso). For this purpose, 400 to 500 juvenile fish with an average weight of approximately 10 gr were divided into 14 tanks and raised for 10 to 14 days to acclimatize to environmental conditions. The treatments included: A single bath vaccination with anti-yersiniosis vaccine (with Brand name: Antiyersin) for 10 gr fish, two-stage bath vaccination against yersiniosis for 10 gr fish. two-stage hyperosmotic bath vaccination against yersiniosis (antiyersin) for 10 gr fish. Single injection vaccination against yersiniosis for 10 gr fish and a control group of unvaccinated fish. The impact of the vaccine on the biochemical factors in the blood of beluga sturgeon was also evaluated. The results indicated that in the vaccinated treatments, biochemical blood indices such as glucose, uric acid, urea, cholesterol, total protein, triglycerides, calcium, creatinine, and albumin were measured. The findings from this study demonstrated that the vaccine had a better effect on the health of beluga sturgeon weighing between 10 to 15 gr, with significant differences observed in glucose, uric nitrogen, uric acid, triglycerides, total cholesterol, and calcium levels between the vaccinated groups and the control group. However, there was no significant effect on total protein, creatinine levels, and the gene expression of dismutase among the vaccinated groups compared to the control group.

The current study was designed to Know the Potassium dichromate (chromium) effects of histological structure on ovaries and thyroid glands in adult female rats. Twenty-one adult female rats were divided into three groups. The first group (n=7) animals received distal water as the control group, the second group (n=7) animals received potassium dichromate dissolved in distal water at a dose (n= 8.5 mg\kg), and the third group (n=7) animals received potassium dichromate at a dose (n= 4.25 mg\kg) dissolved in distal water. Animals received chromium orally by gavage for 30 days. The animals were sacrificed at the last experiment and the ovaries and thyroid have been isolated for histological study. Results showed normal ovarian histological structure in the control group and normal collagen fibers surrounding the vascular tissues. The potassium dichromate-treated group showed cysts, hemorrhage, distortion of granulosa cells, and collagen fibers not appearing with high doses. Histological ovarian sections in the treated group at a low dose of chromium showed normal ovarian structure with few fibroblasts. Normal thyroid follicles appeared in the control group. Thyroid gland structure in the treatment group at high doses of chromium showed the epithelium follicles have hyperplasia with distortion of follicle cells and walls. The follicles in the low dose group of chromium showed damage to follicle cells, vacuolated and limited hyperplasia. Therefore, chromium directly or indirectly affects of ovaries and thyroid glands

This study aimed to determine the prevalence of coccidiosis in broiler farms in Duhok Province, Iraq. A cross-sectional study was performed from June to October 2023. 600 fresh fecal samples were collected from the litters of the suspected farms, and the parasitological examinations were carried out using standard methods by using both direct fecal and flotation fecal methods for searching for coccidian oocysts. Also, 600 intestine and ceca samples were collected from the same chicken for macroscopic examination to report the presence of any pathological changes in the intestine and the ceca. The study findings revealed a high prevalence rate of coccidiosis (60%) among broiler farms in Duhok Province. Regarding ages, the ages 4-6 weeks were observed the most prevalent for infection (66.56%) than in other age groups. This study revealed that the infection rate of coccidiosis was higher in the Autumn season than in the summer season. According to the gross examination, most cases showed positive for Eimeria tenella (4433%), which indicates cecal coccidiosis and (55.7%) were positive with other Eimeria spp. Finally, some positive cases of Eimeria tenella were found infected with other species. The prevalence of coccidiosis among broiler farms was high in this study, especially, cecal coccidiosis and this is associated with several risk factors such as poor management, bad ventilation systems, humidity, and inadequate biosecurity systems in the farms.