Introduction: The development of Jembrana disease vaccine is an important effort to prevent losses in the Bali cattle industry in Indonesia. This study aims to prepare a Jembrana DNA vaccine encoding the transmembrane portion of the envelope protein in pEGFP-C1 and test the success of its delivery in culture cells using a chitosan-DNA complex. Material and Methods: Cloning of the DNA vaccine was successfully performed on E. coli DH5α and confirmed by colony PCR, restriction analysis and sequencing. The plasmids were prepared as a chitosan complex using the complex coacervation method and physicochemically characterised using a particle size analyser. A transfection assay was performed in HeLa cells with 4 h exposure, and mRNA expression was assessed at 24 h post transfection. Results: With a 1:2 (wt./wt.) ratio of DNA and chitosan, the complexes have a mean diameter of 236 nm, zeta potential value of + 17.9 mV, and showed no high toxicity potential in the HeLa cells. This complex successfully delivered the DNA into cells, as shown by the presence of a specific RT-PCR product (336 bp). However, the real-time PCR analysis showed that the delivery with chitosan complex resulted in lower target mRNA expression when compared with a commercial transfecting agent. Conclusion: pEGFP-env-tm JDV as a candidate vaccine can be delivered as the chitosan-DNA complex and be expressed at the transcription level in vitro. This initial study will be used for further improvement and evaluation in vivo.

Introduction: Campylobacter jejuni is one of the most frequently reported causes of foodborne bacterial enteric disease worldwide. The main source of these microorganisms is contaminated food, especially of poultry origin. There are several molecular methods for differentiation of Campylobacter isolates at the subgenus level, and one of these is porA-typing based on the sequencing of the major outer-membrane protein (MOMP) encoding gene. The aim of the study was to test the molecular relationship of C. jejuni strains isolated at different points along the poultry food chain and assess the population structure of the isolates. Material and Methods: A total of 451 C. jejuni were used in the study, and a DNA fragment of 630 bp of the MOMP encoding gene was amplified and sequenced. Results: One hundred and ten sequence types were identified, with 69 (62.7%) unique to the isolates' origin and 30 not present in the database. The most prevalent nucleotide variant 1 was detected in 37 (8.2%) strains. These isolates were identified in all poultry sources tested, especially in faeces (15 isolates) but also in poultry carcasses and meat (11 isolates in each). Conclusion: The porA typing method was highly discriminative for C. jejuni of poultry origin since the Simpson's diversity index (D) achieved a value of 0.876, indicating considerable diversity in the bacterial population tested. The method may be further used for epidemiological investigation purposes.

Introduction: Laryngeal swab samples collected from three waterfowl slaughterhouses in central Taiwan were cultured and suspected isolates of Riemerella anatipestifer were identified by API 20NE and 16S rDNA PCR. Material and Methods: Serum agglutination was used for serotyping, and antimicrobial susceptibility was tested. Results: Seventy-six R. anatipestifer isolates were detected, and the prevalences in the ducks and geese were 12.3% (46/375) and 8.0% (30/375), respectively. The positive isolation rates were 65.6% for all arriving waterfowl, 76.0% for birds in the holding area, 1.6% for defeathered carcasses, but zero for degummed carcasses. A PCR examination detected R. anatipestifer in the slaughtering area frequently. Serotype B was dominant in both duck (34.8%) and goose (46.7%) isolates, but the wide serotype distribution may very well impede vaccination development. All isolates were resistant to colistin, and 79.7% were resistant to more than three common antibiotics. Conclusion: The results proved that most ducks had encountered antibiotic-resistant R. anatipestifer in rearing, which suggests that the bacterium circulates in asymptomatic waterfowl. It is worth noting that most waterfowl farms were found to harbour R. anatipestifer, and contaminated slaughterhouses are a major risk factor in its spread. Effective prevention and containment measures should be established there to interrupt the transmission chain of R. anatipestifer.

Introduction: The aim of this research was to provide a detailed description of the morphology, topography, and histometry of rabbit accessory genital glands. Material and Methods: Seven male New Zealand White rabbits, 3–4 months of age and weighing 2.1–3 kg were used for the study. The whole urethra from the urinary bladder to the external urethral orifice accompanied by accessory genital glands was sliced at intervals of 1 mm. The serial sections were prepared with haematoxylin-eosin (H&E) and Movat–Russell modified pentachrome stain. Results: A detailed description of the morphology and morphometry was provided. The topography of the organs was explained on the basis of characteristic cross-sections on histological slides. The inconsistent nomenclature and descriptions of these glands by different authors were also discussed. Conclusion: The morphometric analysis indicated that some of the glands described have similar dimensions in different individuals, while others like paraprostates revealed high diversity in the number of lobes, their size, and their structure. The accessory glands are also good topographic markers which precisely define the segment of the urethra. The terms “proprostate”, “prostate”, and “paraprostates” as the nomenclature of the prostate complex reflect the location of these glands well and indicate their common origin and function.

This study, the Turkish version of the STROBE-VET was made to strengthen the reporting of observational studies in the veterinary fieldand making the usage of the STROBE-VET better understood by the researchers. Firstly, STROBE-VET expression translated into Turkishby 4 authors (TB, FG, ÖK, MAT) each from the other and afterwards four translated versions have been combined with consensus by experttranslator. The final version of criteria was translated into English by another expert translator using reverse translation method and found to bequiet similar between translated of Turkish control list and the original control list. Secondly, in order to assess the Turkish control list whetherit is understood correctly by the researchers, 20 studies in the field of veterinary sciences have been evaluated by two veterinarians (DÖ andMBC). The correspondence between the responses of the researchers was examined using Cohen's Kappa statistic ( ) and the percentage ofcompliance between researchers for each criterion. In addition, it was assessed using the Bland-Altman Method and the Intraclass CorrelationCoefficient (ICC) using the total number of reported criteria. According to results, criteria has a great variation in the frequency of reportingin the studies. The Turkish version of STROBE-VET will be a guide for the referees and editors of journals as well as researchers studying inthe field of veterinary science.

Mycoplasma bovis is one of the most important pathogens causing pneumonia worldwide. In this study, it was aimed to determine intercellular adhesion molecule-1 (ICAM-1) and C-reactive protein (CRP) levels in calves infected with Mycoplasma bovis. The animal material of the study consisted of 30 calves including 20 simmental calves with pneumonia due to Mycoplasma bovis at 2-4 months of age and 10 healthy calves at the same age and the race. Serum levels of intercellular adhesion molecule-1 (ICAM-1) and C-reactive protein (CRP) were determined in calves infected M. bovis and control group. Serum levels of intercellular adhesion molecule-1 (ICAM-1) and C-reactive protein (CRP) were found to be statistically higher in the calves with pneumonia than in the control group (P<0.01). A positive correlation between serum ICAM-1 concentrations and Mycoplasma bovis titers (increases in coefficient ratios) were statistically significant (r=0.664), indicating a positive correlation between serum ICAM-1 and CRP levels and titers of Mycoplasma bovis in the calves with pneumonia. As a result, it was observed that serum intercellular adhesion molecule-1 (ICAM-1) and C-Reactive Protein (CRP) concentrations were increased in infected pneumonia with Mycoplasma bovis. It is considered useful to consider these parameters in evaluating the prognosis of pneumonia cases of Mycoplasma bovis origin and the efficacy of applied treatment.

In the study, the data obtained to describe the body characteristics of the Hairpin were utilized in the businesses that were registered with Karaman Province Breeding Sheep Goat Breeders Association. Body weights of 130 goats, 2, 3, 4, 5, 6 and 7 years old and 50 goats, 2, 3 and 4 years old, selected by simple random sampling method were used in the data of total 900. In the study, Pearson correlation coefficient for variables providing parametric test prerequisites, and Spearman correlation analysis for variables not providing parametric test prerequisites. In the regression analysis, "live weight" dependent variable and other variables were determined as independent variables and parametric and nonparametric regression methods were applied. Univariate and multivariable regression models were applied for the whole data set. When all analyzes are evaluated, univariate regression models give lower determination coefficients (R2) than multivariate models. In this case, it has been deemed appropriate to use a multivariate regression model instead of a univariate model in order to make a correct prediction.However, in practice, univariate Quadratic or Cubic regression methods can be used for researchers.

This study was carried out to understand how sausage matrices affect the survival and acid tolerance of STEC O157 and O26. STEC O157 and O26 were inoculated on sausage surface approximately 5 log. After inoculation sausages were vacuum packed and stored at 4°C. Pathogen counts and synthetic gastric fluid (pH 1,5) experiments were conducted on day 0, 15 and 30 of the storage. Three trials were conducted for each pathogen separately. Both serogroups had viable counts on sausage during storage, STEC O26 count decreased about 1log and O157 about 3log during storage. At the end of the storage both O26 and O157 were viable on the sausage surface 4.59 log and 2.54 log respectively. For acid survival experiments pathogen counts were obtained on 30th, 60th and 90th minute of synthetic gastric fluid (SGF) exposure. Our results show that O26 endured acid stress longer than O157 during SGF experiments throughout storage of frankfurters. The results of this study may support the idea that some non-O157 STEC strains might be more resistant to acid stress than O157 STEC but further studies should be conducted before drawing a conclusion.

This study was carried out to investigate the socio-economic analysis of fishermen of Çıldır Lake (Ardahan/Kars) cooperative. It has beendetermined that all fishermen are members of these cooperatives and that they are not members of any other nongovernmental organization. Ithas been determined that the ages of fishermen vary between 34 and 74, 100% are married and their spouses are between 20 and 70 years old.They were found to be primary school graduates with a rate of 74%. 65.21% of fishermen received a special training on their professions. Itwas found that 17.4% of fishermen had no sources of income other than fishing, and the remaining 82.6% were active in agriculture, animalhusbandry or other fields. It is generally determined that they have 1 boat, the majority of the boats are 6 m in length and made of iron. It wasseen that 43.47% of family members participated in hunting activities. Ownership of all of the boats belonged to the fishermen themselves and30.4% of them were informed that they were receiving them with the help of their boat families. It was found that fishermen used pinters incrayfish hunting and fennel nets in fish hunting. Although Çıldır Lake fishermen 's professions are found to be economically inadequate, it hasbeen determined that 14 fishermen with 60.87% of their income are happy to do this job due to their presence in one or more of the reasons foradditional income, habit, passion / hobby.

In the world, aquaculture is seen as the fastest growing and developing food production sector and however, in the near future, it is estimatedthat the rates of fisheries and aquaculture will be equalized. In 2015, the fisheries production of the world amounted to 170,345,641 tons (fisheries;93,704,616 tons and aquaculture; 76,641,025 tons) according to the FAO records.The aquaculture in Turkey started in 1970s and its rate in total fisheries production has rapidly increased as of 2000. In our country, the amountof aquaculture, which was 79,943 tons in 2003, reached 253,395 tons in 2016. The aquaculture rate in the total production of our country hasincreased by more than 310%. As one of the most important reasons for this rate increase, it is seen to be providing some support policies withvarious incentive systems by the government for support and encourage the aquaculture in country.Directly product support for aquaculture farmers was started in 2003. According to the total capacity of the farms, total product and fry supportswere provided. In 2008, the total capacity supported was limited to 2000 tons/year. However, in 2012, different tonnage application (whole unitprice to 250 tons/year and ½ unit price from 251 to 500 tons/year) were brought to the total product support and the fry support was removed.At the same time, the processed product support was brought at the rate of twice the amount specified in the invoices of the total product. In thepresent, the support system is still continuing.The types of product support, prices, supported capacities and the expense criteria are applied annually by issuing the “Aquaculture SupportCommuniqués” in the Official Gazette.