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Expression of bovine leukaemia virus (BLV) gp51 protein in blood and milk cells of cows with leukosis Полный текст
2022
Szczotka Maria | Kuźmak Jacek
Bovine leukaemia virus (BLV) is the retroviral causative agent of enzootic bovine leukosis, the most common neoplastic disease of cattle and a serious problem worldwide. Its diagnosis is commonly by tests for antibodies recognising the p24 capsid protein and structural glycoprotein (gp) 51. With flow cytometry recently having come to veterinary immunology, applications for it may now include BLV. The study determined BLV gp51 expression in blood and milk lymphocytes of naturally infected cows by flow cytometry.
Показать больше [+] Меньше [-]Is serological monitoring a fit-for-purpose tool to assess the epidemiological situation of tuberculosis in the sylvatic species of European bison (Bison bonasus) in Poland? Полный текст
2022
Krzysiak Michał K. | Larska Magdalena | Zabost Anna | Didkowska Anna | Krajewska-Wędzina Monika | Anusz Krzysztof | Augustynowicz-Kopeć Ewa
Bovine tuberculosis is one of the most dangerous zoonotic diseases. Despite the near-complete elimination of the disease from cattle breeding in Poland achieved in 2009, its re-emergence is now observed. Globally, the number of human cases is underestimated and the importance of free-living animals as reservoirs of tuberculosis is growing. As a species highly susceptible to Mycobacterium tuberculosis complex infection, the European bison (Bison bonasus) has a role in the transmission of the disease in Poland. The purpose of the investigation was to assess the epidemiological situation of tuberculosis in Polish European bison serologically.
Показать больше [+] Меньше [-]Examination of immunogenic properties of recombinant antigens based on p22 protein from African swine fever virus Полный текст
2022
Díaz Carlos | Salát Jiří | Břínek Kolařová Dagmar | Celer Vladimír | Frébort Ivo
The single member of the Asfarviridae family is African swine fever virus (ASFV). This double-stranded DNA virus infects wild and farmed swine and loses the pig industry large sums of money. An inner envelope, capsid, and outer envelope are parts of the ASFV particle containing structural proteins playing different roles in the process of infection or host immune defence evasion. When expressed by the baculovirus system, the p22 protein from the inner envelope was found to induce partial protection against a virulent virus strain. This study aimed to express a part of this protein in a different system and evaluate its immunogenicity.
Показать больше [+] Меньше [-]Transcriptome analysis of bovine macrophages (BoMac) cells after infection with bovine immunodeficiency virus Полный текст
2022
Rola-Łuszczak Marzena | Materniak-Kornas Magdalena | Kubiś Piotr | Pluta Aneta | Smagacz Marlena | Kuźmak Jacek
Bovine immunodeficiency virus (BIV) is found worldwide in cattle under natural conditions. However, the effect of BIV infection on immune functions has not been fully characterised.
Показать больше [+] Меньше [-]Analysis of antimicrobial resistance and genetic correlations of Escherichia coli in dairy cow mastitis Полный текст
2022
Li Ke | Hou Mingyuan | Zhang Lin | Tian Mengyue | Yang Ming | Jia Li | Liang Yanyan | Zou Dongmin | Liu Ruonan | Ma Yuzhong
Escherichia coli is a widespread environmental pathogen frequently causing dairy cow mastitis. This bacterium is particularly capable of acquiring antimicrobial resistance, which can have severe impacts on animal food safety and human health. The objective of the study was to investigate antimicrobial resistance and genetic correlations of E. coli from dairy cow mastitis cases in northern China.
Показать больше [+] Меньше [-]Detection and antimicrobial resistance of Enterobacteriaceae other than Escherichia coli in raccoons from the Madrid region of Spain Полный текст
2022
Antonio Orden José | Martínez-Rodrigo Abel | Isabel Vela Ana | Francisco Fernández-Garayzábal José | Hurtado-Morillas Clara | Mas Alicia | Domínguez-Bernal Gustavo
Raccoons are an invasive alien species widely distributed in the Madrid region of Spain. These animals can carry a variety of enteric bacteria with associated antimicrobial resistance, which can infect humans and livestock. However, to our knowledge, the presence of non-E. coli Enterobacteriaceae in raccoons has not been previously studied.
Показать больше [+] Меньше [-]Vaccine-induced rabies in a red fox in Poland Полный текст
2022
Smreczak, Marcin | Orłowska, Anna | Müller, Thomas | Freuling, Conrad Martin | Kawiak-Sadurska, Magdalena | Trębas, Paweł
Vaccine-induced rabies in a red fox in Poland Полный текст
2022
Smreczak, Marcin | Orłowska, Anna | Müller, Thomas | Freuling, Conrad Martin | Kawiak-Sadurska, Magdalena | Trębas, Paweł
Introduction: Rabies as a zoonosis threatens public health worldwide. Several thousand people die each year of infections by the rabies virus (RABV). Oral rabies vaccination (ORV) of wildlife was successfully implemented in many European countries and led to rabies being brought under control there. In Poland, ORV was introduced in 1993 using vaccines containing an attenuated strain of the rabies virus. However, attenuated rabies viruses may have residual pathogenicity and cause the disease in target and non-target animals. Material and Methods: A red fox carcass was tested as part of national rabies surveillance, and its brain was screened for RABV infection using two conjugates and a fluorescent antibody test (FAT). The rabies virus was isolated in mouse neuroblastoma cells by rabies tissue culture infection test (RTCIT), and viral RNA was detected by heminested reverse transcriptase PCR (hnRT-PCR) as well as by quantitative real-time RT-PCR (rtRT-qPCR). An amplicon of 600 bp was subjected to Sanger sequencing. To differentiate between vaccine and field RABV strains, PCR-restriction fragment length polymorphism (PCR-RFLP) using the Dra I, Msp I, Nla IV and Mbo II restriction endonucleases was performed. Results: The rabies virus was detected in the fox’s brain using FAT, RTCIT and molecular tests. The PCR-RFLP revealed of vaccine-induced rabies, and full-length genome analysis showed 100% nucleotide sequence identity of the isolate with the reference sequences of Street Alabama Dufferin Bern (SAD Bern) vaccine strains and other vaccine-induced rabies virus isolates detected in animals and deposited in GenBank. Conclusion: We detected vaccine-induced rabies for the first time in Poland in a fox during routine rabies surveillance.
Показать больше [+] Меньше [-]Vaccine-induced rabies in a red fox in Poland Полный текст
2022
Smreczak Marcin | Orłowska Anna | Müller Thomas | Freuling Conrad M. | Kawiak-Sadurska Magdalena | Trębas Paweł
Rabies as a zoonosis threatens public health worldwide. Several thousand people die each year of infections by the rabies virus (RABV). Oral rabies vaccination (ORV) of wildlife was successfully implemented in many European countries and led to rabies being brought under control there. In Poland, ORV was introduced in 1993 using vaccines containing an attenuated strain of the rabies virus. However, attenuated rabies viruses may have residual pathogenicity and cause the disease in target and non-target animals.
Показать больше [+] Меньше [-]ASF -survivors’ sera do not inhibit African swine fever virus replication in vitro Полный текст
2022
Walczak, Marek | Juszkiewicz, Małgorzata | Szymankiewicz, Krzesimir | Szczotka-Bochniarz, Anna | Woźniakowski, Grzegorz
ASF -survivors’ sera do not inhibit African swine fever virus replication in vitro Полный текст
2022
Walczak, Marek | Juszkiewicz, Małgorzata | Szymankiewicz, Krzesimir | Szczotka-Bochniarz, Anna | Woźniakowski, Grzegorz
African swine fever virus (ASFV) causes one of the most dangerous diseases of pigs and wild boar – African swine fever (ASF). Since its second introduction into Europe (in 2007), the disease has been spreading consistently, and now ASF-free European countries are at risk. Complex interactions between the host’s immune system and the virus have long prevented the development of a safe vaccine against ASF. This study analysed the possibility of neutralisation of the ASFV in vitro by sera collected from ASF-survivor animals. Two pig and three wild boar serum samples were collected from previously selected potential ASF survivors. All sera presented high antibody titres (>5 log₁₀/mL). Primary alveolar macrophages were cultured in growth medium containing 10% and 20% concentrations of selected sera and infected with a haemadsorbing ASFV strain (Pol18_28298_O111, genotype II). The progress of infection was investigated under a light microscope by observing the cytopathic effect (CPE) and the haemadsorption phenomenon. Growth kinetics were investigated using a real-time PCR assay. Haemadsorption inhibition was detected in the presence of almost all selected sera; however, the inhibition of virus replication in vitro was excluded. In all samples, a CPE and decreasing quantification cycle values of the viral DNA were found. Anti-ASFV antibodies alone are not able to inhibit virus replication. Interactions between the humoral and cellular immune response which effectively combat the disease are implicated in an ASF-survivor’s organism.
Показать больше [+] Меньше [-]ASF -survivors’ sera do not inhibit African swine fever virus replication in vitro Полный текст
2022
Walczak Marek | Juszkiewicz Małgorzata | Szymankiewicz Krzesimir | Szczotka-Bochniarz Anna | Woźniakowski Grzegorz
African swine fever virus (ASFV) causes one of the most dangerous diseases of pigs and wild boar – African swine fever (ASF). Since its second introduction into Europe (in 2007), the disease has been spreading consistently, and now ASF-free European countries are at risk. Complex interactions between the host’s immune system and the virus have long prevented the development of a safe vaccine against ASF. This study analysed the possibility of neutralisation of the ASFV in vitro by sera collected from ASF-survivor animals.
Показать больше [+] Меньше [-]Nephrin and podocin mRNA detection in urine sediment of dogs with chronic kidney disease: preliminary observations Полный текст
2022
Souza, Camilla de | Coelho, Mariana | Antonelo, Daniel Silva | Passarelli, Danielle | Rochetti, Arina Lázaro | Fukumasu, Heidge | Leite-Dellova, Deise Carla Almeida
Nephrin and podocin mRNA detection in urine sediment of dogs with chronic kidney disease: preliminary observations Полный текст
2022
Souza, Camilla de | Coelho, Mariana | Antonelo, Daniel Silva | Passarelli, Danielle | Rochetti, Arina Lázaro | Fukumasu, Heidge | Leite-Dellova, Deise Carla Almeida
Dogs with chronic kidney disease (CKD) may have alterations in the glomerular filtration barrier, including podocyte loss. Detection of podocyte mRNA in urine could be useful for assessing podocyturia in dogs with kidney disease. The objective of this study was to evaluate the presence of nephrin mRNA (NPHS1) and podocin mRNA (NPHS2) in urine sediments of dogs with naturally occurring CKD and healthy dogs. Twenty-four dogs, 14 with CKD and 10 as healthy controls, underwent clinical evaluation. The dogs with CKD were divided into two groups, according to the International Renal Interest Society criteria: stage 1 or 2 CKD (n = 5) and stage 3 or 4 CKD (n = 9). Urine was collected by catheterisation or free catch and RNA isolation from the urine sediments was optimised using glycogen as a co-precipitant. Detection of NPHS1 and NPHS2 in the sediment samples was performed using quantitative real-time PCR. Both types of mRNA were detected in samples from all groups, but the percentages of detection were higher in the group of dogs with stage 1 or 2 CKD and lower in the group of dogs with stage 3 or 4 disease. Physiological podocyturia was observed in healthy dogs, and the results suggest differential podocyturia in dogs with CKD, according to the stage of the disease, i.e. an increase in podocyturia in dogs at stage 1 or 2 and a reduction in podocyturia in dogs at stage 3 or 4.
Показать больше [+] Меньше [-]Nephrin and podocin mRNA detection in urine sediment of dogs with chronic kidney disease: preliminary observations Полный текст
2022
Souza Camilla de | Coelho Mariana | Antonelo Daniel Silva | Passarelli Danielle | Rochetti Arina Lázaro | Fukumasu Heidge | Leite-Dellova Deise Carla Almeida
Dogs with chronic kidney disease (CKD) may have alterations in the glomerular filtration barrier, including podocyte loss. Detection of podocyte mRNA in urine could be useful for assessing podocyturia in dogs with kidney disease. The objective of this study was to evaluate the presence of nephrin mRNA (NPHS1) and podocin mRNA (NPHS2) in urine sediments of dogs with naturally occurring CKD and healthy dogs.
Показать больше [+] Меньше [-]Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region Полный текст
2022
Ijiri, Moe | Odo, Kenji | Sato, Motohiko | Kawaguchi, Maiko | Fujimoto, Yoshikazu | Miura, Naoki | Matsuo, Tomohide | Hou, De-Xing | Yamato, Osamu | Tanabe, Takashi | Kawaguchi, Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region Полный текст
2022
Ijiri, Moe | Odo, Kenji | Sato, Motohiko | Kawaguchi, Maiko | Fujimoto, Yoshikazu | Miura, Naoki | Matsuo, Tomohide | Hou, De-Xing | Yamato, Osamu | Tanabe, Takashi | Kawaguchi, Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region were investigated by comparing the biomarker changes in the summer (a period of chronic heat stress) and winter (a thermoneutral period) seasons. Pigs were allocated to summer- and winter-finishing cohorts, 12 each. The evaluations included assessment of carcass traits and internal organs’ normality carried out at the time of slaughter, and measurement of biomarkers in whole blood: derivatives of reactive oxygen metabolites (d-ROMs) and biological antioxidant potential as markers of oxidative stress, and serum amyloid A and albumin/globulin (A/G) ratio as markers of acute and chronic inflammation, respectively. The summer-finished pigs reared under subtropical field conditions showed lower carcass quality than the winter-finished pigs, indicating a potential adverse effect of summer temperatures on the swine industry. Marginal changes were observed in d-ROMs and the A/G ratio between the summer- and winter-finishing cohorts. The results demonstrate that d-ROMs and the A/G ratio could be used as sensitive markers for heat stress under field conditions.
Показать больше [+] Меньше [-]Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region Полный текст
2022
Ijiri Moe | Odo Kenji | Sato Motohiko | Kawaguchi Maiko | Fujimoto Yoshikazu | Miura Naoki | Matsuo Tomohide | Hou De-Xing | Yamato Osamu | Tanabe Takashi | Kawaguchi Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region were investigated by comparing the biomarker changes in the summer (a period of chronic heat stress) and winter (a thermoneutral period) seasons.
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