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What Constitutes Effective Interprofessional Education Amongst Health Related Disciplines : A Literature Review Полный текст
2019
Wardani, Elly | Rachmah, R. | Mayasari, Putri | Mahdarsari, Mayanti
Interprofessional Education (IPE)is considered an educational strategy that has potential benefit forimproving teamwork and collaboration in practice, leading to improved patient outcomes. As the development and implementation of curricula that integrate IPE requires significant resources, its adoption should be based on evidence of effectiveness. The purpose of this literature review is to identify what constitutes effective interprofessional education amongst health related disciplines. A literature search was conducted using Science Direct, BMC, Elsevier and Joint Commission Journal for the years 2009-2018. A total of 15 studies met the established inclusion criteria. The use of IPE among health related disciplines is one effective way to solve several problems, and its particularly improves patient safety. What constitutes effective interprofessional education amongst health related disciplines are possibly viewed from some aspects, such as efficiency, effectiveness, learning strategy, power, patient and learner safety and readiness to practice, interprofessional curriculum, roles and relationships, and learning outcomes.
Показать больше [+] Меньше [-]Lactic Acid Bacteria and Histamine Levels of Sie Balu After Gamma Irradiated. Полный текст
2019
Arham, Arham | Nurliana, Nurliana | Sugito, Sugito
The presence of lactic acid bacteria (LAB) and histamine in foodstuffs indicate the level of deterioration in the quality of food and cause poisoning.Sie Baluis the Acehnese dried meat preserved by the addition of salt, acid and dried, but the long processing and drying it under the sun can cause microbial contamination in meat products. Irradiation can eliminate bacteria in foodstuffs. This study aimed to determine the amount of LAB and histamine levels ofSie Baluafter irradiation doses of 5, 7 and 9 kGy and stored 2 to 4 months.Sie Baluwas made of fresh beef 5 kg, dried in the sun to dry, vacuumed and irradiated with gamma rays. The samples for LAB determination cultured in MRS agar and incubated at 37C for 24 hours. The number of colonies was counted using Total Plate Count. The histamine level ofSie Baluconducted by ELISA. Irradiation did not significantly (P0.05) affect the amount of LAB, but the shelf life significantly (P0.05) affected the amount of LAB inSie Balu. Extending the shelf life up to 4 months can increase the amount of LAB. Irradiation dose and shelf life had no effect on histamine levels ofSie Balu(P0.05). This study concluded that irradiatedSie Balucannot be stored for more than two months.
Показать больше [+] Меньше [-]Antibiotic Activities To Staphylococcus aureus Phospholipase Of Aceh Cattle Preputium Isolate Полный текст
2019
Dewi, Maryulia | Adhayani, Layli | Zuraidawati, Zuraidawati | Erina, Erina
This study aimed to study the effect of antibiotic on phospholipase production ofStaphylococcus aureusisolated from preputium of Aceh cattle. The parameters measured in this study were PzI ofStaphylococcus aureuswithout any treatment, and PzI ofS. aureusafter being given antibiotics. The experiment was carried out by modifying the Samaranayake method on egg yolk agar media, incubated at 37 C for 48 hours, and the precipitation lines formed was measured. The antibiotics used were tetracycline, oxytetracycline and fosfomycin with concentrations of 10%, 20% and 30% (mg / mL, respectively). The results showed that phospholipase production ofS. aureusisolated from preputium of Aceh cattle was suppressed, antibiotics were able to inhibit phospholipase production. However, the enzyme was still produced in positive category, with PzI = 0.287, the highest result was found in 30% tetracycline activity, which is equal to PzI = 0.341 and the lowest was in 10% fosfomycin which is equal to PzI = 0.332
Показать больше [+] Меньше [-]Detection of native interferon-γ in nyala (Tragelaphus angasii): Towards diagnosing tuberculosis Полный текст
2019
Roux, Lezaan(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases, Bovine Tuberculosis and Brucellosis Research Programme) | McCall, Alicia J.(Veterinary Services Department of Agriculture and Rural Development) | Michel, Anita L.(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases, Bovine Tuberculosis and Brucellosis Research Programme)
Mycobacterium bovis is the main cause of tuberculosis in wildlife. In South Africa, African buffaloes (Syncerus caffer) are a wildlife maintenance host while a number of other species are considered spillover hosts. Nyala (Tragelaphus angasii), a large antelope species from Southern Africa, is frequently traded and can be infected with M. bovis. Interferon gamma (IFN-γ) release assays that detect cell-mediated immune (CMI) responses to M. bovis infection have shown promise in elephants, rhinoceroses and buffaloes. The BOVIGAM® assay is a commercial IFN-γ release assay designed to detect tuberculosis in cattle and has been validated in buffaloes. We tested the suitability of the BOVIGAM® assay to detect native IFN-γ release in nyala. Blood samples collected from 17 nyalas were stimulated with different mitogens and IFN-γ release measured. We found that incubating whole blood with phorbol 12-myristate 13-acetate and calcium ionophore (PMA/CaI) resulted in the highest levels of IFN-y release. Samples stimulated with tuberculin purified protein derivatives of M. bovis (PPDb) and M. avium (PPDa) did not show significant IFN-γ production. An intradermal tuberculin test (IDT) and culture of tissues from 15 of the 17 culled nyala were also performed, which supported the findings of the BOVIGAM® assay, suggesting the potential value of this assay for the diagnosis of tuberculosis in nyala.
Показать больше [+] Меньше [-]The Influence of Geographic Area on Blood Parameters of Pramenka Sheep in the Area of Bosnia and Herzegovina Полный текст
2019
Hrkovic, Amina | Vegara, Mensur | Hodzic, Aida | Pasic, Eva | Softic, Almira | Ohran, Husein
The aim of this study was to determine the influence of geographical area (central and southwestern part of Bosnia and Herzegovina) on hematological and biochemical blood parameters of autochthonous Pramenka sheep. Materials and The study included 104 sheep from the Vlasic mountain (central part) (n = 52) and Livno (southwestern part) (n = 52). Blood samples were taken from the jugular vein into Vactuainer tubes with EDTA anticoagulant for hematological, for glucose, analyses and BD Vacutainer® SST II gel for biochemical analyses. All hematological and biochemical analyses were performed within the next 24 hours, and until then the samples were kept at 4 °C. Hematological parameters included total Red Blood Cell (RBC), White Blood Cell (WBC), Hemoglobin concentration (Hb), Hematocrit (Hct), Mean Corpuscolar Hemoglobin (MCH), Mean Corpuscolar Hemoglobin Concentration (MCHC), Mean Corpuscular Volume (MCV), Mean Platelet Volume (MPV), Red Cell Distribution Width (RDW) and White Cell Differential Count (WCDC). Analyzes are carried out using the automated veterinary hematological analyzer Advia 120 SIEMENS. Blood in the serum tubes was allowed to clot for at least 30 min prior to centrifugation. Serum samples were kept frozen at -20 °C until biochemical analyses were performed. Biochemical parameters were determined by analyzer Olimpus AU400 with Beckman Coulter reagens according to the manufacturer’s protocol. Parameters for biochemistry panel included: total protein (TP), albumin (ALB), globulin (GLO), urea (BUM), creatinine (CRE), glucose (GLU), aspartate aminotransferase (AST), γ-glutamyltransferase (GGT), creatine kinase (CK), cholesterol (CHO), bilirubin (BIL), calcium (Ca), phosphorus (P),sodium (Na), chloride (Cl), potassium (K) and magnesium (Mg). All analyzes were tested to spectrophotometric method, except for Na, K, Cl, which were generated by ISE method, or by ion selective method. BHBA and NEFA were constructed with reagents from Randox.Results: The values of WBC (p<0.05), RBC (p<0.001), Hb (p<0.001), PCV (p<0.001), MCV (0.05), NEU (p<0.01) from the Livno area, while the value of LYM (p<0.05) was determined for sheep from the Vlasic area. The correlative values between RBC: Hb (P<0.001), RBC:PCV (P<0.001), WBC:NEU (P<0.001), WBC:LYM (P<0.001), WBC:BAS (P<0.001) areas. The correlative correlation at P<0.01 was established between RBC:MCH, RBC:PLT, RBC:MPV in sheep from Vlasica area, while correlative values at P<0.05 were established between RBC:MPC, WBC:MON for sheep from the Livno area. The values of BHB (p<0.001), total protein (p<0.001), albumin (p<0.001), urea (p<0.001), AST (p<0.001), cholesterol (p<0.001) , magnesium (p<0.001) were determined for sheep in area Livno. The values of NEFA (p<0.001), creatinine (p<0.01), glucose (p<0.001), bilirubin (p<0.001), phosphate (p<0.001) were established for sheep in the Vlasic area. Correlative correlation (P<0.001) between total protein:chloride, calcium:phosphates, sodium:chlorides was found in animals from Vlasic area, while correlation was found (P<0.05) between sodium:chloride in animals from the Livno area.It was concluded that values showed significant differences for individual haematological and biochemical parameters in sheep for both investigated areas.
Показать больше [+] Меньше [-]Comparison of Some Bacterial Identification Methods Полный текст
2019
Gulaydin, Ozgul | Eki̇n, Ismail Hakki | Oztürk, Cihat | Ilhan, Ziya | Ogun, Erdal
In this study, three different methods were compared for the identification of some Gram-positive and Gramnegative reference bacteria. Material and Methods: For this purpose, the identification accuracy rates of Enterococcus faecalis, Enterococcus faecium, Streptococcus agalactiae, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Serratia marcescens were analysed by conventional bacteriological methods, commercial bacterial identification test kit (Microgen™ ID) and automated bacteria identification system (BD Phoenix 100™).
Показать больше [+] Меньше [-]The Microbiological Quality of Tantuni Полный текст
2019
Selçuk, Ümit | Ağaoğlu, Sema
In this study, microbiological quality of tantuni that have been consumed in the province of Van was examined. Materials and Methods: For this purpose; 100 tantuni samples, whose 79 of them were red meat tantuni (raw and cooked) and 21 of them chicken tantuni (raw and cooked) were used as material.According to analysis findings with regard to aerobic mesophilic organisms, coliform group microorganisms, E. coli, micrococcus/staphylococcus, S. aureus, C. perfringens and yeast-mould at the samples of raw and cooked red meat tantuni were found to be 5.67 and 3.98, 2.88 and 0.21, 0.87 and 2.00, 2.99 and 2.27, 1.33 and 0.25, 0.05 and 1.00, 4.43 and 0.63 log cfu/g respectively. In the same order, at the samples of raw and cooked chicken tantuni were found to be; 4.35 and 3.77, 2.84 and 1.00, 1.15 and 2.00, 0.95 and 1.22, 2.00 and 2.00, 1.00 and 1.00, 4.05 and 0.11 log cfu/g respectively. Salmonella spp. could not be isolated in the tantuni samples that had been investigated. In the raw red meat tantuni samples 5.26% (1/19) had S. aureus, in the cooked red meat tantuni samples 1.66% (1/60) had S. aureus, and 3.33% (2/60) yeast-mould which were not compatible with the limit values that were stated at the Turkish Food Codex were found. However, values obtained from this study show that during preparation and production of the goods and in the other stages; hygienic rules have not been carried out.In conclusion to secure the product safety, it is essential to be cautious for the temperature and time during preparation, reservation temperature and GMP/GHP based applications in the preparation of tantuni.
Показать больше [+] Меньше [-]African animal trypanosomosis (nagana) in northern KwaZulu-Natal, South Africa: Strategic treatment of cattle on a farm in endemic area Полный текст
2019
Latif, Abdalla A.(University of KwaZulu-Natal School of Life Sciences) | Ntantiso, Lundi(Makhathini Research Station) | de Beer, Chantel(Agricultural Research Council-Onderstepoort Veterinary Research)
African animal trypanosomosis (nagana) in northern KwaZulu-Natal, South Africa: Strategic treatment of cattle on a farm in endemic area Полный текст
2019
Latif, Abdalla A.(University of KwaZulu-Natal School of Life Sciences) | Ntantiso, Lundi(Makhathini Research Station) | de Beer, Chantel(Agricultural Research Council-Onderstepoort Veterinary Research)
African animal trypanosomosis (AAT) is caused by several species of the genus Trypanosoma, a parasitic protozoan infecting domestic and wild animals. One of the major effects of infection with pathogenic trypanosome is anaemia. Currently, the control policies for tsetse and trypanosomosis are less effective in South Africa. The only response was to block treat all infected herds and change the dip chemical to one which controls tsetse flies during severe outbreaks. This policy proved to be less effective as demonstrated by the current high level of trypanosome infections in cattle. Our objective was to study the impacts of AAT (nagana) on animal productivity by monitoring the health of cattle herds kept in tsetse and trypanosomosis endemic areas before and after an intervention that reduces the incidence of the disease. The study was conducted on a farm in northern KwaZulu-Natal which kept a commercial cattle herd. There was no history of any cattle treatment for trypanosome. All cattle were generally in poor health condition at the start of the study though the herd received regular anthelminthic treatment. A treatment strategy using two drugs, homidium bromide (ethidium) and homidium chloride (novidium), was implemented. Cattle were monitored regularly for 13 months for herd trypanosomosis prevalence (HP), herd average packed cell volume (H-PCV) and the percentage of the herd that was anaemic (HA). A total of six odour-baited H-traps were deployed where cattle grazed from January 2006 to August 2007 to monitor the tsetse population. Glossina brevipalpis Newstead and Glossina austeni Newstead were collected continuously for the entire study period. High trypanosomes HP (44%), low average H-PCV (29.5) and HA (24%) were rerecorded in the baseline survey. All cattle in the herd received their first treatment with ethidium bromide. Regular monthly sampling of cattle for the next 142 days showed a decline in HP of 2.2% - 2.8%. However, an HP of 20% was recorded by day 220 and the herd received the second treatment using novidium chloride. The HP dropped to 0.0% and HA to 0.0% by day 116 after the second treatment. The cow group was treated again by day 160 when the HP and HA were 27.3% and 11%, respectively. The same strategy was applied to the other two groups of weaners and the calves at the time when their HP reached 20%. Ethidium and novidium treatment protected cattle, that were under continuous tsetse and trypanosomosis challenge, for up to 6 months. Two to three treatments per year may be sufficient for extended protection. However, this strategy would need to be included into an integrated pest management approach combining vector control for it to be sustainable.
Показать больше [+] Меньше [-]African animal trypanosomosis (nagana) in northern KwaZulu-Natal, South Africa: Strategic treatment of cattle on a farm in endemic area Полный текст
2019
Abdalla A. Latif | Lundi Ntantiso | Chantel de Beer
African animal trypanosomosis (AAT) is caused by several species of the genus Trypanosoma, a parasitic protozoan infecting domestic and wild animals. One of the major effects of infection with pathogenic trypanosome is anaemia. Currently, the control policies for tsetse and trypanosomosis are less effective in South Africa. The only response was to block treat all infected herds and change the dip chemical to one which controls tsetse flies during severe outbreaks. This policy proved to be less effective as demonstrated by the current high level of trypanosome infections in cattle. Our objective was to study the impacts of AAT (nagana) on animal productivity by monitoring the health of cattle herds kept in tsetse and trypanosomosis endemic areas before and after an intervention that reduces the incidence of the disease. The study was conducted on a farm in northern KwaZulu-Natal which kept a commercial cattle herd. There was no history of any cattle treatment for trypanosome. All cattle were generally in poor health condition at the start of the study though the herd received regular anthelminthic treatment. A treatment strategy using two drugs, homidium bromide (ethidium) and homidium chloride (novidium), was implemented. Cattle were monitored regularly for 13 months for herd trypanosomosis prevalence (HP), herd average packed cell volume (H-PCV) and the percentage of the herd that was anaemic (HA). A total of six odour-baited H-traps were deployed where cattle grazed from January 2006 to August 2007 to monitor the tsetse population. Glossina brevipalpis Newstead and Glossina austeni Newstead were collected continuously for the entire study period. High trypanosomes HP (44%), low average H-PCV (29.5) and HA (24%) were rerecorded in the baseline survey. All cattle in the herd received their first treatment with ethidium bromide. Regular monthly sampling of cattle for the next 142 days showed a decline in HP of 2.2% – 2.8%. However, an HP of 20% was recorded by day 220 and the herd received the second treatment using novidium chloride. The HP dropped to 0.0% and HA to 0.0% by day 116 after the second treatment. The cow group was treated again by day 160 when the HP and HA were 27.3% and 11%, respectively. The same strategy was applied to the other two groups of weaners and the calves at the time when their HP reached 20%. Ethidium and novidium treatment protected cattle, that were under continuous tsetse and trypanosomosis challenge, for up to 6 months. Two to three treatments per year may be sufficient for extended protection. However, this strategy would need to be included into an integrated pest management approach combining vector control for it to be sustainable.
Показать больше [+] Меньше [-]Serum biochemistry in cows of different breeds presented with reproductive conditions Полный текст
2019
Molefe, Keitiretse(North-West University Faculty of Natural and Agricultural Science Department of Animal Health) | Mwanza, Mulunda(North-West University Faculty of Natural and Agricultural Science Department of Animal Health)
Serum biochemistry in cows of different breeds presented with reproductive conditions Полный текст
2019
Molefe, Keitiretse(North-West University Faculty of Natural and Agricultural Science Department of Animal Health) | Mwanza, Mulunda(North-West University Faculty of Natural and Agricultural Science Department of Animal Health)
Minimising health problems and increasing yield have always been the objectives in livestock agriculture. Hence, increases in incidences of reproductive conditions in cattle farming pose a great threat to productivity and impose undesirable economic implications. This study aimed to examine the concentrations of different biochemical compounds in cows with reproductive conditions. Seventy-seven blood samples were collected from cows at different rural areas around Mafikeng, following cases of downer cow syndrome, dystocia, retained placenta, vaginal prolapse and abortion. Means of serum metabolites across the different reproductive conditions were statistically compared using Pearson's chi-square test to determine variations of serum metabolites in cows of different breeds. In mixed breed cows, higher than normal calcium concentrations were observed in downer cow syndrome (25.25 ± 8.47) and dystocia (85.50 ± 8.46) cases. It was also observed that cholesterol concentrations were significantly low in abortion (2.52 ± 0.79), retained placenta (3.18 ± 0.61) and vaginal prolapse (2.37 ± 0.97) cases in Afrikaner cows. The study showed that Brahman (43.1%) and Afrikaner (43.1%) breeds were mostly affected by downer cow syndrome. Additionally, the occurrences of downer cow syndrome (53.9%) and abortions (60%) were mostly observed in cows of 1-3 years, in second and first parities, respectively. This study proves that concentrations of calcium, urea or blood urea nitrogen (BUN), magnesium and cholesterol are significantly altered in incidences of reproductive conditions in cows of different breeds. It is also shown that serum biochemistry is affected by reproductive conditions in cows of different ages and parity. This data serves as a tool that could be used to enhance research in animal production and reproduction.
Показать больше [+] Меньше [-]Serum biochemistry in cows of different breeds presented with reproductive conditions Полный текст
2019
Keitiretse Molefe | Mulunda Mwanza
Minimising health problems and increasing yield have always been the objectives in livestock agriculture. Hence, increases in incidences of reproductive conditions in cattle farming pose a great threat to productivity and impose undesirable economic implications. This study aimed to examine the concentrations of different biochemical compounds in cows with reproductive conditions. Seventy-seven blood samples were collected from cows at different rural areas around Mafikeng, following cases of downer cow syndrome, dystocia, retained placenta, vaginal prolapse and abortion. Means of serum metabolites across the different reproductive conditions were statistically compared using Pearson’s chi-square test to determine variations of serum metabolites in cows of different breeds. In mixed breed cows, higher than normal calcium concentrations were observed in downer cow syndrome (25.25 ± 8.47) and dystocia (85.50 ± 8.46) cases. It was also observed that cholesterol concentrations were significantly low in abortion (2.52 ± 0.79), retained placenta (3.18 ± 0.61) and vaginal prolapse (2.37 ± 0.97) cases in Afrikaner cows. The study showed that Brahman (43.1%) and Afrikaner (43.1%) breeds were mostly affected by downer cow syndrome. Additionally, the occurrences of downer cow syndrome (53.9%) and abortions (60%) were mostly observed in cows of 1–3 years, in second and first parities, respectively. This study proves that concentrations of calcium, urea or blood urea nitrogen (BUN), magnesium and cholesterol are significantly altered in incidences of reproductive conditions in cows of different breeds. It is also shown that serum biochemistry is affected by reproductive conditions in cows of different ages and parity. This data serves as a tool that could be used to enhance research in animal production and reproduction.
Показать больше [+] Меньше [-]Prevalence of Giardia and Cryptosporidium in young livestock and dogs in Magude District of Maputo Province, Mozambique Полный текст
2019
Miambo, Regina D.(Eduardo Mondlane University Faculty of Veterinary Medicine Department of Para-Clinics,University of KwaZulu-Natal College of Agriculture, Engineering and Science School of Life Science) | Laitela, Benigna(Eduardo Mondlane University Faculty of Veterinary Medicine Department of Para-Clinics) | Malatji, Mokgadi P.(University of KwaZulu-Natal College of Agriculture, Engineering and Science School of Life Science) | Afonso, Sonia M. de Santana(Eduardo Mondlane University Faculty of Veterinary Medicine Department of Para-Clinics) | Junior, Alberto P.(Eduardo Mondlane University Faculty of Veterinary Medicine Department of Para-Clinics) | Lindh, Johan(Uppsala University Department of Cell and Molecular Biology) | Mukaratirwa, Samson(University of KwaZulu-Natal College of Agriculture, Engineering and Science School of Life Science)
Prevalence of Giardia and Cryptosporidium in young livestock and dogs in Magude District of Maputo Province, Mozambique Полный текст
2019
Miambo, Regina D.(Eduardo Mondlane University Faculty of Veterinary Medicine Department of Para-Clinics,University of KwaZulu-Natal College of Agriculture, Engineering and Science School of Life Science) | Laitela, Benigna(Eduardo Mondlane University Faculty of Veterinary Medicine Department of Para-Clinics) | Malatji, Mokgadi P.(University of KwaZulu-Natal College of Agriculture, Engineering and Science School of Life Science) | Afonso, Sonia M. de Santana(Eduardo Mondlane University Faculty of Veterinary Medicine Department of Para-Clinics) | Junior, Alberto P.(Eduardo Mondlane University Faculty of Veterinary Medicine Department of Para-Clinics) | Lindh, Johan(Uppsala University Department of Cell and Molecular Biology) | Mukaratirwa, Samson(University of KwaZulu-Natal College of Agriculture, Engineering and Science School of Life Science)
BACKGROUND: Giardia and Cryptosporidium species are significant zoonotic parasites of humans and domesticated animals. OBJECTIVES: The study aimed to determine the prevalence of Giardia and Cryptosporidium in livestock and dogs of the Magude District. METHOD: The flotation technique (Willis), modified Ziehl-Neelsen (mZN) and direct and indirect immunofluorescence (DIF and IIF) techniques were applied to determine the prevalence of Giardia and Cryptosporidium species in faecal samples of dog pups (156), goat kids (60) and calves (480) from the Magude District of Mozambique from February to September 2015. RESULTS: Using Willis, IIF and DIF, the prevalence of Giardia in calves was 0%, 8.1%, and 6.0%; in dogs 0.6%, 8.3% and 5.7% and for goats 0% and 13.3% (IIF was not performed), respectively. The prevalence of Cryptosporidium in calves using Willis, mZN, IIF and DIF was 0%, 3.8%, 4.7% and 0.4% and in dogs 0%, 0.6%, 6.4% and 0.6%, respectively. The parasite was not detected in goats. CONCLUSION: Results from the present study showed that IIF performed better diagnosis of Giardia and Cryptosporidium, and that the mZN can be used as an alternative for Cryptosporidium because of the high cost of IIF. There is a need for identification of genotypes or subtypes of these parasites through application of molecular techniques in order to determine their zoonotic potential, and we advocate a 'one health' approach in the control and prevention of these parasites.
Показать больше [+] Меньше [-]Prevalence of Giardia and Cryptosporidium in young livestock and dogs in Magude District of Maputo Province, Mozambique Полный текст
2019
Regina D. Miambo | Benigna Laitela | Mokgadi P. Malatji | Sonia M. de Santana Afonso | Alberto P. Junior | Johan Lindh | Samson Mukaratirwa
Background: Giardia and Cryptosporidium species are significant zoonotic parasites of humans and domesticated animals. Objectives: The study aimed to determine the prevalence of Giardia and Cryptosporidium in livestock and dogs of the Magude District. Method: The flotation technique (Willis), modified Ziehl-Neelsen (mZN) and direct and indirect immunofluorescence (DIF and IIF) techniques were applied to determine the prevalence of Giardia and Cryptosporidium species in faecal samples of dog pups (156), goat kids (60) and calves (480) from the Magude District of Mozambique from February to September 2015. Results: Using Willis, IIF and DIF, the prevalence of Giardia in calves was 0%, 8.1%, and 6.0%; in dogs 0.6%, 8.3% and 5.7% and for goats 0% and 13.3% (IIF was not performed), respectively. The prevalence of Cryptosporidium in calves using Willis, mZN, IIF and DIF was 0%, 3.8%, 4.7% and 0.4% and in dogs 0%, 0.6%, 6.4% and 0.6%, respectively. The parasite was not detected in goats. Conclusion: Results from the present study showed that IIF performed better diagnosis of Giardia and Cryptosporidium, and that the mZN can be used as an alternative for Cryptosporidium because of the high cost of IIF. There is a need for identification of genotypes or subtypes of these parasites through application of molecular techniques in order to determine their zoonotic potential, and we advocate a ‘one health’ approach in the control and prevention of these parasites.
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