Ranula rarely resolve without surgery and marsupialisation is easily applicable technique to manage sublingual salivary mucocele. A two year old male German shepherd dog was presented to Veterinary Clinical Complex, Veterinary College and Research Institute, Namakkal with the history of swelling on ventral aspect of the tongue for the past one and half month. The fine needle aspiration cytology revealed macrophages mixed with coccobacillary clumps, haematobiochemical parameters were within the physiological range. Based on the clinical signs, the case was diagnosed as ranula. Under atropine sulphate and xylazine hydrochloride premedication, induction of anaesthesia with ketamine and diazepam followed by isoflurane maintenance, marsupialization was done.  Postoperatively, the animal was treated with broad spectrum antibiotic and antiinflammatory drugs for five days, and the animal recovered uneventfully after 15 days.

Leptospirosis is under diagnosed and under reported in developing countries due to lack of diagnostic facilities. The diagnosis of leptospirosis is complex, because the gold standard tests are not easily available. Though rapid tests are available for the past two decades, they have to be combined with the existing gold standard tests for confirmation of diagnosis. It should be adequate to diagnose leptospirosis with rapid tests. I intend to review the literature from developing countries in Asia, Latin America and Africa and suggest the need for utilizing rapid tests for diagnosis. Leptospirosis can be diagnosed only by diagnostic tests and epidemiological data, such as incidence/ prevalence data cannot be obtained without these tests. Without these data, funding cannot be obtained for diagnostic, treatment and control programs, as without diagnostic tests, leptospirosis would be considered a rare disease. In this article, the various guidelines for the diagnosis of leptospirosis will be discussed.

Present study was undertaken to evaluate the shelf life of chhana podo prepared from 1% citric acid and 2% lactic acid and stored at ambient temperature (30+ 20 C). Microbial, physico-chemical and sensory qualities of product were studied during storage. The chhana podo were analyzed Standard Plate Count (SPC), yeast and mould count on alternate days. The SPC, yeast and mould count increased significantly (P<0.05) with the progress of storage at ambient temperature in 1% citric acid and 2% lactic acid products. No significant change in SPC, yeast and mould count was observed up to 2nd day storage but later there was remarkable increase in SPC (2.79 & 2.55) as well as yeast and mould count (12.40 & 12.30) at the end of day four. The significant reduction in pH, moisture and fat content in 1% citric acid and 2% lactic acid products during the progress of storage of 8 days, while protein content showed an increasing trend. All the sensory quality attributes of products made by 1% citric acid and 2% lactic acid were comparable up to 2 days of storage from then on declined significantly with progress of storage. Colour, body & texture, flavour and overall acceptability score of channa podo declined significantly up to the 4th days of storage. Thus, the findings of the study indicated that the channa podo could be safely stored upto 4th days at ambient temperature.  

Bacterial infection followed by implant fixation is a common complication after surgery. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. In this study, the effect of in vitro antibacterial activity of magnetron sputtered silver nanoparticles was studied. Sputtering was done using radiofrequency magnetron sputtering technology. Film applicator coating assay was used to assess the antibacterial effect of the coated titanium implants. SEM examination revealed successful deposition of silver nanoparticles on the titanium surface. The average diameter of the nanoparticles was 40-60 nm. SEM examination before incubation and after incubation of bacteria was done. The bactericidal ratio between the uncoated and coated implant was determined.

Bacteriological quality of khoa based sweets marketed through different retail outlets of Thrissur city was evaluated based on total viable count, coliform count, yeast & mould count and staphylococcal count. Altogether 72 samples of khoa based sweets such as peda, burfi and gulabjamun were evaluated for their bacteriological quality. Out of the total peda samples tested, coliforms were prevalent in 37.5%, yeast and mould in 91.7% and staphylococcal organisms in 41.7% of the samples. In burfi, coliforms were prevalent in 66.6%, yeast and mold in 95.8% and staphylococcal organisms in 83.3 % of the samples tested. In gulabjamun, coliforms, yeast and mould and staphylococcal organisms were present in 45.8%, 91.6% and 70.8% respectively of the samples tested. The highest rate of contamination was recorded in burfi while the lowest was recorded in peda. Strict hygienic measures during preparation and storage are recommended to ensure the quality of the products. 

Nanotechnology has been used in a variety of medical applications, including diagnostics, immunisation, medication and gene delivery. Nanotechnology has opened up new ways for molecular biology and biotechnology applications, and it has revolutionised practically every area of veterinary and animal sciences by introducing new, small-scale instruments and materials that are advantageous to living species. Metallic nanoparticles, quantum dots, carbon nanotubes, magnetic nanoparticles, fullerenes,  liposomes, and dendrimers are among the several forms of nanomaterials employed in detection of various diseases, therapy, drug administration, animal nutrition and breeding. It is concluded that, before making nano-products available for animal use, extensive risk evaluations should be carried out to ensure their safety. So, this article provides an overview of existing and promising nano applications in veterinary research that may lead to increased efficiency and production.

Leptospirosis is one of the most important zoonotic disease caused by the pathogenic leptospires Leptospira interrogans. In this study, four pathogenic Leptospira serovars were selected based on the seroprevalence data, their LPS extracted, assessed for its purity and seroreactivity and processed for the determining the fatty acid profiles. The average concentration of the LPS extracted from the cultures across the serovars was 742.75+19.4 µg/ml with the polysaccharides contributing to 77.2 % of the LPS (573.6+18.01 µg/ml). The common fatty acids that were present in all the four serovars included butyric acid (C4:0), capric acid (C10:0), palmitoleic acid (C16:0),  eicosapentaenoic acid (C20:5n3) and docosapentaenoic acid (C22:6n3). The heptadecanoic acid (C17:1) was present in serovars australis and autumnalis; pentadecanoic acid (C15:0) in canicola and hardjo serovars and caproic acid (C6:0) in the serovars autumnalis and canicola. The fatty acid eicosatrinoic acid (C20:3n3) was unique to the serovar autumnalis and the myristic (C13:0) and docosadienoic acid (C22:2) being unique to the serovar hardjo. Future studies to understand the fatty acid profile of the other common pathogenic circulating Leptospira LPS will add scientific evidence to the minor cross-reactivity observed across the serovars in MAT.

A pregnant graded Murrah buffalo at eight months of gestation in third parity having bilateral distension of abdomen was presented. The case was diagnosed as hydramnios and treated with dinoprost tromethamine, diethyl stilbesterol, valethamate bromide and dexamethasone. After 76 hours, cervix was dilated fully and trans-cervical amniocentesis was performed and approximately 60-70 litres of amniotic fluid was drained and a dead male fetus with cleft palate was delivered.

A one year old, non descript doe presented with the history of straining to deliver and hanging of ruptured water bag from the vulva was diagnosed as dystocia. The attempts for manual extraction of foetus were failed and a dead emphysematous male foetus was removed by caesarean section. Hydrocephalus and prognathism were observed in the dead foetus. The doe uneventfully recovered postoperatively.

A pluriparous full-term pregnant buffalo was presented with a history of unproductive straining with improper relaxation of the vulva. Detailed obstetrical examination revealed the presence of two fetuses that were conjoined twins with attachment present at the sternal region of the thorax of the fetuses. The conjoined twins (Ziphopagus monster) were delivered per-vaginal by episiotomy technique.