Research on determination of changes of morphological and biochemical blood indexes of sheep with fasciolosis after application of the drug Klozantsid (menizol 20%, produced in the Republic of Belarus) was carried out. The research realized in the conditions of the Scientific and Research Institute of Applied Veterinary Medicine and Biotechnology of the Vitebsk State Academy of Veterinary Medicine. In course of the experiment there were used sheep of Romanovskaya breed at the age from 1,5 till 5 years which were spontaneously infected with fasciola. The experimental animals were administrated with the examined antihelminthic preparation. It was found out that after administration of the preparation there was stated the restoration of a level of erythrocytes, haemoglobin, leukocytes, enzymes, proteins and other blood indexes. Klozantsid did not render toxic influence on studied animals. | Проведены исследования по выяснению изменений морфологических и биохимических показателей крови у овец с фасциолёзной инвазией при применении препарата Клозанцид. Исследования проводили в условиях клиники и лаборатории кафедры паразитологии и инвазионных болезней, лаборатории кафедры клинической диагностики, научно-исследовательского института прикладной ветеринарной медицины и биотехнологии учреждения образования «Витебская ордена «Знак Почёта» государственная академия ветеринарной медицины». Объектом исследования являлись овцы романовской породы в возрасте от 1,5 до 5 лет, спонтанно зараженные фасциолами, которым применяли испытуемый препарат, обладающий антигельминтными свойствами. При этом выяснено, что после введения препарата происходит восстановление уровня эритроцитов, гемоглобина, лейкоцитов, ферментов, белков и других показателей. Клозанцид не оказывал токсического влияния на организм животного.

Morphological and biological characteristic of isospora and eimeria was realized in the conditions of the Republic of Belarus on the basis of carried out measurements and experimental infection minks (Mustela). Research results showed, that in Belarus at mink breeding farm holdings there were determined four species of eimeria and isospores, one of which I. eversmanni - is registered by researchers from the Vitebsk State Academy of Agriculture in the Republic for the first time. The most widespread species is Е. vison (57,03%) was found out in all analyzed 24 fur breeding farms. Species composition of eimeria and isospores did not depend on a sex and typical coat pattern of minks, as well as on a geographical location area of an analyzed farm. Some distinctions in species composition of the intestinal protozoa were observed at minks of different age. Of all analyzed species the most pathogenic proved to be Е. vison and I. laidlawi which in case of infectioning caused animal mortality. | Дана морфологическая и биологическая характеристика изоспор и эймерий на основании проведенной микрометрии и экспериментального заражения норок. Таким образом, по результатам проведенных нами исследований следует отметить, что в Республике Беларусь у норок, разводимых в хозяйствах, установлено четыре вида эймерий и изоспор, один из которых - I. eversmanni - зарегистрирован нами в республике впервые. Самым распространенным видом является Е. vison (57,03%), обнаруженный во всех обследованных нами 24-х зверохозяйствах. Видовой состав эймерий и изоспор не зависит от пола и типовой окраски норок, а также от географического месторасположения хозяйства. Некоторые различия в видовом составе кишечных простейших наблюдались у норок разного возраста. Из отмеченных видов наиболее патогенными являются Е. vison и I. laidlawi, вызывающие при заражении падеж зверей.

Despite of presence of a wide choice of antihelminthics, in the Republic of Belarus it is still a real problem of development of preparations that provide long-term treatment and preventive maintenance efficiency against helminthosis. At the same time, helminthes and anthelminthics render the immunosuppression action. The solution of this problem can be a combined application of antihelminthics and adjuvants. There were proposed preparations Albepolis and Averpolis. Their application made it possible not only to release an organism of animals from parasites, but also to correct the state of immune system. The obtained research data confirm the available data about the immunosuppression action of parasites on an organism of animals. There was also is noted the immunosuppression action of Albendazol and Aversektin C in therapeutic doses. Albendazol possess a wide spectrum of action against trematodes, nematodes, cestodes. It interrupts the metabolism, oppresses fumarate reductase activity and adenosine triphosphate synthesis of a parasite. It proved to be low-toxic for homoiothermal animals. Albendazol rendered positove influence on growth, development and increase of cattle productivity.

In the conditions of the Republic of Belarus there was determined the parasitizing influence of Oesophagostomum and their larvae on a metabolism of experimentally infested animals. In course of the study there were generated experimental and control groups of two-month old piglets. Animals of the experimental group were infested in a dose of 15000 invasion larvae per one kg of body weight. Animals of the control group were not infested. Infestation was realized through a mouth with invasion larvae with wet forage. Invasion larvae received by cultivation of excrement tests from infested pigs at temperature of 24 deg C. As a result of the realized research it was established, that Oesophagostomum invasion rendered a substantial influence on the experimental animals. At bimestrious pigs after the experimental infection with Oesophagostomum larvae there were stated the following changes: the disease on 4-30th day was characterized by frustration of function of a gastroenteric path - diarrhea, fever, anaemia, and in the subsequent - stagnation and juvenilism of animals. Oesophagostomum in a host organism caused the pathological changes of haemotological and biochemical blood value which were expressed in quantity decrease of erythrocytes, concentration of haemoglobin, leucocytosis. Eosinophilia was observed in a leukogram. In blood serum there was stated the lowering of crude protein and its fractions content; gradually, but authentically there was noted the increasing of activity serum glutamic oxalacetic transaminase and glutamic-pyruvic transaminase. Bactericidal and lysozymic activity of blood serum decreased. Activity of alkaline phosphatase increased.

In the conditions of the Republic of Belarus there was studied the preventive efficiency of various forms of a complex additive Felutsen against polymicroelementoses of calf-bearing dry cows and its influence on some of biochemical blood indexes. Research results proved high prophylactic efficacy of various forms of the additive Felutsen against polyhypomicroelementoses of calf-bearing dry cows. Restoration of a microelement homeostasis of cows made it possible to normalize the functional activity of liver, to prevent development renal insufficiency and dystrophic changes in bone tissue. The most expressed metabolic changes in blood of calf-bearing dry cows were stated after application of the carbohydrate-mineral additive Felutsen-briquette universal. There was given the analysis of structure of studied forms of complex additive Felutsen; indexes of trace element metabolism in cows; indexes of protein metabolism and level of nonprotein nitrogen; indexes of blood characterizing the functional condition of liver and carbohydrate metabolism of cows; indexes of trace element metabolism in cows after the termination of application of the additive.

In the conditions of the Republic of Belarus there was studied the influence of herbal infusion, tincture, liquid and dry extracts of elecampane (Inula helenium) on morphological and biochemical indexes of pig blood. In course of the study it was established, that preparative forms of elecampane stimulated the formation of blood corpuscle, and also increased of the hemoglobin level in blood of pigs. Administration of elecampane preparations did not cause any authentic changes in blood leukogram of swine; all indicators were within normal limits. Elecampane preparations did not cause changes on the part of biochemical blood indexes of and did not influence the activity of enzymes. Research results showed that herbal infusion, tincture, liquid and dry extracts of elecampane did not render negative influence on swine.

In the conditions of the Republic of Belarus there was realized the determination of efficiency of application of saline and chelate forms of copper and cobalt for treatment of anemic syndrome of cattle. It was found out that lack of copper and cobalt in cattle diets in the conditions of normal supply of iron could be a cause of cattle anemic syndrome. It took place because of the iron adsorption disorder in intestines and its inclusions in haem, as well as lowering of ehritropoztin synthesis. In the conditions of administration of copper and cobalt-containing preparations for animals there was noted the increasing of iron and haemoglobin content in blood. Administration of ethylene diamine tetraacetates of cobalt and copper made it possible to reduce considerably all evidences of clinical and laboratory symptoms of anemia and hypomicroelementosis in comparison with application of copper sulphate and cobalt.

In the conditions of the Republic of Belarus there was studied the antihelmintic characteristics of the preparative forms of marsh cinquefoil (Comarum palustre): herbal infusion, infusion, liquid, extract, pulvis. The research has shown that these preparative forms in the specified dozes had a rather high therapeutic efficiency against strongylatosis of gastro-intestinal tract of young ruminants. The highest therapeutic effect was rendered by a liquid extract of Comarum palustre after its administration to animals in a dose of 0,2 ml/kg twice with 24 hour interval. In this case during the strongylatoses of a gastroenteric tract the antiparasitic intensefficiency of sheep and cattle by the end of experience was 98,1% and 97,3% respectively, and antiparasitic extensefficiency was 90% for both kinds of animals. After herbal infusion application of Comarum palustre for animals (in a dose of 7 ml/kg of live weight before feeding once a day within 3 days continuously) by the end of the 14 day of the experiment made it possible to obtain intensefficiency of 70% for both animal species, and extensefficiency of 60% for sheep and 70% - at calves. Tincture application of marsh cinquefoil for animals in a dose of 1 ml/kg of live weight of twice with 24 hours interval, provided the intensefficiency of 85% for sheep and 84% - for cattle, and extensefficiency of 70% and 80%, respectively. In the conditions of application of a pulvis of marsh cinquefoil in a dose of 500 mg/kg of live weight twice with 24 hours interval, made it possible to obtain the 63,4% intensefficiency for sheep and 67,8% - for calves, and extensive efficiency - 50% and 40%, respectively.

In the conditions of the Republic of Belarus there were studied the biochemical changes in blood of cows with chronic dystrophic processes in liver by an example of fatty hepatodystrophy. There was given an estimation of these changes taking into account animal physiological state (pregnancy). The metabolic profile including the enzymatic block from aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, cholinesterase, serum albumin, total cholesterin, total bilirubin and thymol test can be used in clinical and biochemical diagnostics in case of hepatic steatosis. Its changes in case of hepatic steatosis were characteriszed by increase in activity of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, decrease in activity of cholinesterase, decrease in blood serum of albumins concentration, increase in general cholesterol and high values of thymol tests. Determination of gamma-glutamyl transpeptidase influence and flocculation test on apo-beta-lipoproteins showed that they should not be included in this complex due to their small information content. In the process of estimation of changes of a metabolic profile of blood serum it wasnecessary to consider the physiological state of animals (pregnancy) because the activity of some enzymes and metabolite concentration at them could essentially differ. Taking into consideration the character of hepatic steatosis course, the studied metabolic profile can be recommended for hyposthenic current chronic activity with the considerable involvement degree of hepatocytes into the process.

In the conditions of the Republic of Belarus there was realized an experimental study of a new method treatment of calves diseased with abomasoenteritis by means of application of a preparation Ranitidin. As a result of realized research it was established that the preparation had an expressed therapeutic activity. Inclusion of Ranitidin preparation into the complex therapeutic regimen of calves sick with abomasoenteritis with the enteral feeding once in day in a dose of 0,15 g promoted the reduction of duration and decrease in severity of the disease. There were presented the results of biochemical blood indicators in trial and control groups of calves, haematologic indices in trial and control groups of calves prior to the beginning of experiment and in the end of the test.