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Phylogenetic analysis of Fasciola spp. isolated from slaughtered cattle in KwaZulu-Natal and Mpumalanga provinces of South Africa based on the cytochrome c oxidase subunit I mitochondrial marker Полный текст
2019
Tatenda J. Chikowore | Oliver T. Zishiri | Samson Mukaratirwa
Phylogenetic analysis of Fasciola spp. isolated from slaughtered cattle in KwaZulu-Natal and Mpumalanga provinces of South Africa based on the cytochrome c oxidase subunit I mitochondrial marker Полный текст
2019
Tatenda J. Chikowore | Oliver T. Zishiri | Samson Mukaratirwa
Fasciola spp. are the causative agents of fascioliasis in humans and livestock. Before the development of control and management measures, the geographical distribution of the species and patterns of infection must be considered. Because of difficulties in the phenotypic differentiation and morphometric classification of Fasciola spp., DNA molecular markers have become more useful for fluke differentiation and description of phylogenetic patterns. This study aimed to differentiate and describe the phylogenetic background of Fasciola spp. isolated from cattle slaughtered at three abattoirs in the Mpumalanga and KwaZulu-Natal provinces of South Africa. The cytochrome c oxidase I (COI) – FHCO1 (forward: 5′-TTGGTTTTTTGGGCATCCT-3′) and FHCO1 (reverse: 5′ -AGGCCACCACCAAATAAAAGA3′) – marker was sequenced from 55 Fasciola flukes that were collected from abattoirs in catchment areas of the KwaZulu-Natal and Mpumalanga provinces. Fasciola hepatica was demonstrated to have 100% prevalence in KwaZulu-Natal and Mpumalanga (highveld), respectively, and 76% prevalence in the lowveld (Belfast area) of Mpumalanga. Two animals from the Belfast metapopulation were co-infected with both Fasciola gigantica and F. hepatica. DNA sequence analysis of all the isolates demonstrated a sequence conservation of 0.472, nucleotide diversity of 0.082 and Tajima’s D of -1.100; however, it was not statistically significant (p > 0.05). Twenty-two haplotypes were identified, with 18 novel haplotypes being unique to the isolates from South Africa. Within the study samples, 12 haplotypes were isolated to a few individuals, with a haplotype diversity of 0.8957 indicating high genetic diversity. Principal coordinate analysis supported the clustering and distribution of the haplotypes, with 11.38% of the variation being attributed to coordinate 2 and 55.52% to coordinate 1. The distribution of Fasciola spp. has been demonstrated to be related to the distribution of the freshwater intermediate host snails, Lymnaea spp., as well as the relative altitude of the localities in South Africa. Information provided by this study serves as preliminary evidence for further studies on the mapping of the distribution of F. gigantica and F. hepatica in South Africa, which is key in designing control programmes for fascioliasis in humans and livestock.
Показать больше [+] Меньше [-]Phylogenetic analysis of Fasciola spp. isolated from slaughtered cattle in KwaZulu-Natal and Mpumalanga provinces of South Africa based on the cytochrome c oxidase subunit I mitochondrial marker Полный текст
2019
Chikowore, Tatenda J.(University of KwaZulu-Natal College of Agriculture, Engineering and Science School of Life Sciences) | Zishiri, Oliver T.(University of KwaZulu-Natal College of Agriculture, Engineering and Science School of Life Sciences) | Mukaratirwa, Samson(University of KwaZulu-Natal College of Agriculture, Engineering and Science School of Life Sciences)
Fasciola spp. are the causative agents of fascioliasis in humans and livestock. Before the development of control and management measures, the geographical distribution of the species and patterns of infection must be considered. Because of difficulties in the phenotypic differentiation and morphometric classification of Fasciola spp., DNA molecular markers have become more useful for fluke differentiation and description of phylogenetic patterns. This study aimed to differentiate and describe the phylogenetic background of Fasciola spp. isolated from cattle slaughtered at three abattoirs in the Mpumalanga and KwaZulu-Natal provinces of South Africa. The cytochrome c oxidase I (COI) - FHCO1 (forward: 5′-TTGGTTTTTTGGGCATCCT-3′) and FHCO1 (reverse: 5′ -AGGCCACCACCAAATAAAAGA3′) - marker was sequenced from 55 Fasciola flukes that were collected from abattoirs in catchment areas of the KwaZulu-Natal and Mpumalanga provinces. Fasciola hepatica was demonstrated to have 100% prevalence in KwaZulu-Natal and Mpumalanga (highveld), respectively, and 76% prevalence in the lowveld (Belfast area) of Mpumalanga. Two animals from the Belfast metapopulation were co-infected with both Fasciola gigantica and F. hepatica. DNA sequence analysis of all the isolates demonstrated a sequence conservation of 0.472, nucleotide diversity of 0.082 and Tajima's D of -1.100; however, it was not statistically significant (p > 0.05). Twenty-two haplotypes were identified, with 18 novel haplotypes being unique to the isolates from South Africa. Within the study samples, 12 haplotypes were isolated to a few individuals, with a haplotype diversity of 0.8957 indicating high genetic diversity. Principal coordinate analysis supported the clustering and distribution of the haplotypes, with 11.38% of the variation being attributed to coordinate 2 and 55.52% to coordinate 1. The distribution of Fasciola spp. has been demonstrated to be related to the distribution of the freshwater intermediate host snails, Lymnaea spp., as well as the relative altitude of the localities in South Africa. Information provided by this study serves as preliminary evidence for further studies on the mapping of the distribution of F. gigantica and F. hepatica in South Africa, which is key in designing control programmes for fascioliasis in humans and livestock.
Показать больше [+] Меньше [-]Antimicrobial usage in pig production: Effects on Escherichia coli virulence profiles and antimicrobial resistance Полный текст
2019
Rukayya H. Abubakar | Evelyn Madoroba | Oluwawemimo Adebowale | Olubunmi G. Fasanmi | Folorunso O. Fasina
Antimicrobial usage in pig production: Effects on Escherichia coli virulence profiles and antimicrobial resistance Полный текст
2019
Rukayya H. Abubakar | Evelyn Madoroba | Oluwawemimo Adebowale | Olubunmi G. Fasanmi | Folorunso O. Fasina
Antimicrobials (AM) are used for growth promotion and therapy in pig production. Its misuse has led to the development of resistant organisms. We evaluated Escherichia coli virulence genes, and compared phenotypic–genotypic antimicrobial resistance (AMR) patterns of faecal E. coli from pigs receiving routine farm treatment without antimicrobial agents against pigs treated routinely with AM over 70 days. Recovered E. coli were tested for AMR using disk diffusion and polymerase chain reaction. Virulence genes were detected in 24.8% of isolates from antimicrobial group and 43.5% from non-antimicrobial group (p = 0.002). The proportion of virulence genes heat-stable enterotoxins a b (STa, STb), enteroaggregative heat stable enterotoxin 1 [EAST1] and Shiga toxin type 2e [Stx2e]) were 18.1%, 0.0%, 78.7% and 3.0% for antimicrobial group and 14.8%, 8.5%, 85.1% and 12.7% for non-antimicrobial groups, respectively. Resistance to oxytetracycline was most common (p = 0.03) in samples collected between days 10 and 21. Resistance shifted to amoxicillin on days 56–70, and trimethoprim resistance was observed throughout. Seventeen phenotypic AMR combinations were observed and eight were multidrug resistant. At least one tetracycline resistance gene was found in 63.9% of the isolates. tet (A) (23.3%) was most common in the antimicrobial group, whereas tet (B) (43.5%) was prevalent in the non-antimicrobial group. Usage or non-usage of antimicrobial agents in growing pigs does not preclude virulence genes development and other complex factors may be involved as previously described. Heavily used AM correspond to the degree of resistance and tetracycline resistance genes were detected during the growth phase.
Показать больше [+] Меньше [-]STUDY THE EFFECT OF ISOLATED ALKALOID FROM HALOXYLON SALICORNICUM PLANT ON SOME HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS IN GASTRIC ULCERATION RABBITS INDUCED BY INDOMETHACIN Полный текст
2019
Noor Abdul Amir Jabbar | Arwa H.M. AL-Saeed | Batool Saleh Haddad
This study was undertaken to isolate alkaloids from Haloxylon Salicornicum plant to show the curative effect after short-term daily orall administration for 10 days (300 mg/Kg B.W.) on gastric ulcer,hematological parameters and some biochemical parameters in gastric ulceration rabbits induced by indomethacin.Moreover,some qualitative chemical analysis,thin layer chromatography,UVspectroscopy and FTIR spectroscopy were used to identify alkaloid compound .Rabbits were divided randomly into three groups,6 rabbits in each.Group one received 3 ml normal saline orally (control group).Group two was treated with indomethacin (75 mg/Kg B.W.) (gastric ulceration group).Third group gastric ulceration rabbits post-treated with isolated alkaloid (300 mg/Kg B.W.).The results of the present study indicated that isolated alkaloid has Rf= 0.86,high significant decreased (p≤0.001) in RBC,Hb,PCVand mucin,(P ≤ 0.01) in MCHC and PH,no significant difference (P ≥ 0.05) in MCH in gastric ulceration rabbits compared with control group and observed high significant increased (p≤0.001) in MCV, MDA and gastric juce volume,WBCcount appeared high significant increased (P≤0.01).While, observed high significant increased(P≤ 0.001) in Hb,PCVand mucin,(P≤ 0.01) in RBC,MCHC and pH.Significant increased(P≤0.05) in MCH after treated with isolated alkaloid and high significantly decrease (P ≤ 0.001)in MCV,gastric juce volume and MDA and significant decrease (p≤0.05) inWBC count compared with gastric ulceration group .This study concluded that isolated alkaloid from Haloxylon Salicoricum plant can be used to treat gastric ulcer effectively .
Показать больше [+] Меньше [-]COMPARATIVE STUDY BETWEEN HORMONAL BLOOD SERUM AND OVARIAN FOLLICULAR FLUID DURING SEASON AND OUT SEASON IN BITCHES Полный текст
2019
Alaa Habeeb Abboud | Taher.A. Fahad
The functions of the ovaries are controlled by many exogenous and endogenous factors, including changes in the biochemical and endocrine glands that occur in the follicular fluid during the breeding season in the bitches. The aim of this study was to determine and compare the concentrations some hormonal in the peripheral circulation and follicular fluid of bitches during the breeding season. For this purpose, ovaries collected from adult bitches immediately after ovariectomy, and blood samples were also collected from these bitches before and after season. The follicular fluid and blood serum samples were analyzed for hormonal concentrations using commercial kits. The results showed that the concentrations of estrogen, progesterone and testosterone in blood serum at season (54.31±0.49, 20.75 ±0.12, 0.75±0.05) respectively. While the concentrations of estrogen, progesterone and testosterone in blood serum at out season (10.88±0.39, 0. 61±0.036, 0.063±0.042) respectively.The concentrations of estrogen, progesterone and testosterone in follicular fluid at season (69.9±0.44, 28.46±0.82, 0.331±0.65) respectively. While the concentrations of estrogen, progesterone and testosterone in follicular fluid out season (there are no found ovarian follicles). The present study a significantly higher (P< 0.05) in blood serum and follicular fluid at season than that out season blood serum.
Показать больше [+] Меньше [-]SYZYGIUM AROMATICUM l. AND THEIR EFFECT ON SEIZURED CHICKENS Полный текст
2019
Jian Salam Hasan Ali
Cloves (Syzyguim Aromaticum L.) represent very interesting plant that has been used for many medicinal purposes such as treatment of convulsions due to its potent antioxidant activity. The purpose of this study is to investigate the anti-convulsant effect of aqueous extract of Syzyguim Aromaticum L. (cloves) at the doses (100,200,300 mg/kg), sodium valproate (200mg/kg) act as reference standard on serum total antioxidant capacity (T-AOC), gamma amino butyric acid (GABA) concentration and cholinesterase (ChE) activity in serum and brain, electrolytes (sodium Na+, potassium K+, chloride Cl- and total calcium Ca+2), glucose and total protein in serum of chicks after induction of seizures by pentylenetetrazole (PTZ), a plant used in traditional medicine as anticonvulsant. 72 chicks were recruited in this study were randomly divided into 6 groups of 12 each. Negative control received normal saline, positive control was given only PTZ, group III was given sodium valproate orally. The rest of three groups were given the extract at previous concentrations given through oral role. Phytochemical analysis of the aqueous extract of cloves revealed the presence of psychoactive compounds such as flavonoids, cardiac glycoside, tannins, and alkaloids. The present work suggest that the aqueous extract of plant had increased level of T-AOC,GABA and ChE in serum of chicks, which were decreased due to the susceptibility to PTZ induced seizure, and decreased the concentration of Na+ and glucose in the serum. We reported that the cloves extract had quite low effect against PTZ induced seizure in spite of their positive effect on different biochemical parameters in serum; this may be due to the low concentration of doses used, which may be in adequate to produce obvious behavioral results.
Показать больше [+] Меньше [-]IDENTIFICATION AND SEROTYPING OF SALMONELLA ISOLATES ISOLATED FROM SOME ANIMAL MEAT Полный текст
2019
Alaa A. Ahmed | Mohammed H. Khudor
This research was already prescribed for the identification and serotyping of Salmonella isolates from 205 samples totally different, including frozen chicken meat(thigh, wings, liver, 40 samples for each one), 40 samples of frozen beef meat 4 and fresh beef meat ( liver, muscle, and ground beef , 15 each ) in Basrah throughout the amount between 4th October 2017 to 27th February2018. Results showed that the overall rate of Salmonella isolates were 22.4 percent by conventional victimization isolation of Xylose Lysine Deoxycholate Agar (XLD).The highest percentagewas appeared in liver of imported frozen chicken meat (80%), while the lowest percentage was in liver of local fresh beef (6.6%). The results of identification of Salmonella by conventional biochemical test and API 20 E system were76.0% and 84% , respectively, while the results of PCR technique by using 16srRNA was( 20/20)100% . The result of serotyping on Salmonellaisolates revealed that serotype SalmonellaTyphimurium (40%), whereas the lower number was Salmonella serotype Kentucky (15%).
Показать больше [+] Меньше [-]ANATOMICAL AND HISTOLOGICAL STUDY OF THYMUS GLANDS IN BROILER CHICKS EMBRYO ( Gallus gallus domesticus). Полный текст
2019
The study designed to investigates the anatomical and histological structures of the thymus gland in broiler chick embryos. Ten healthy, normal embryos at 12th and 20th days old chick embryos was used, divided into five embryos for each age. The thymus was well developed at 12th day chick embryo, Anatomically consisted of 6-8 lobes which are similar in both sides .small, translucent bean shaped lobes that became pale in color at 20th day old embryo, these lobes located in both sides of the neck, positioned parallel to the jugular vein and vagus nerve. Histological structures of thymus gland were composed of capsule, cortex and medulla. At 12th day old of chick embryo the thymus was surrounded by a thin connective tissue capsule, which septa extends into the gland stroma to form a lobulation for each lobe, the cortex and medulla was not differentiated at this age. At 20th day old chicks embryo, the lobules start to recognize and composed of external dark stained cortex with a high population of lymphocytes and internal light stained medulla with less abundant of lymphocytes , reticular fiber and epithelial reticular cells with the appearance of Hassall's corpuscles.
Показать больше [+] Меньше [-]COMPARATIVE STUDY OF USE SOMETREATMENTAL REGIMES TOTREAT POSTPARTUM FUNCTIONAL INFERTILITY OF COWS IN BASRA Полный текст
2019
Postpartum an estrus in cows is regarded as one of the most important causes of functional infertility after parturition during reproductive lifespan. Therefor the present study was conducted to induce fertile estrus in anestrus cows which suffered from inactive ovaries (IO) or persistent corpus luteum(PCL) after parturition. The results of current study showed the efficiency of using GnRH or FSH as a hormonal medication to induce fertile estrus with pregnancy which reach 71.42 and 72.22 % respectively, as well as decreasing the length of days open (DO) to lesser than 150 day in both programs in 70.00 and 76.92 % respectively from induced estrus cows. Also the results revealed that the role of PGF2α in induction of fertile estrus with 83.33% of pregnancy in cows which suffered from postpartum PCL and decrease the length of DO in 80.00% from induced estrus cows. The present study improved the efficacy of uterine massage (UM) through rectal palpation to treat cases of PCL in postpartum anestrus cows with 71.42% of pregnancy, and its effect to decrease the length of DO in 60.00% from induced estrus cows.
Показать больше [+] Меньше [-]ABILITY OF Leishmania donovani TO CONGENITAL TRANSMISSION IN EXPERIMENTAL BALB/C MICE Полный текст
2019
The aim of the current study was to investigate the ability of the transmission of Leishmania donovani parasite from pregnant mothers to their embryos in experimental BALB/c mice. After 5 days of copulation males with females,females were injected with 1012 parasite/ml of promastigote stage through the tail vein, which is grown in NNN-medium. This study demonstrated parasite ability to congenital transmission from mothers to their embryos by placenta through the appearance of amastigote in tissue sections of liver and bone marrow of infected mice fetusescompared with uninfected fetuses. I
Показать больше [+] Меньше [-]EVALUATION ROLE OF THE AUTOGENOUS PERITONEUM AS A SCAFFOLDS ON THE HEALING OF ACHILLES TENDONS IN DOGS Полный текст
2019
A H Allawi | L M Alkattan
The objective of the current study was to evaluate the healing of experimentally severed Achilles tendons of the dogs repaired by Kessler suture pattern alone or with autologous peritoneal scaffold. A total of twelve healthy, neurologically and orthopedically local breed dogs were used in this study, which were randomly allocated into two equal groups (6 of each). The 1st group as a control, while the 2nd as a treated group. In both groups the Achilles tendon of one hind limb was completely severed transversely, in 1st group the tendon was repaired by Kessler suture pattern using silk No.3, while in the 2nd group a combination of Kessler suture and autogenous peritoneal scaffold was applied to reinforce the tendon repair. The site of surgery was evaluated clinically, while gross and histological study was performed at 15, 30 and 60 days post-operative days. Results was revealed that autologous peritoneum graft has a remarkable effects on Achilles tendon healing represented by improving fibroblastic proliferation, reduce adhesion, increase tendon strength and improve tendon function when compared with the control group. We concluded that peritoneal graft application has valuable and beneficial effects on treatment of ruptured Achilles tendon in dogs when utilizing it in conjunction with the traditional procedures.
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