Five Korean native goats were used to test the effectiveness of the 2 modified techniques (Method 1 and 2) of Pawlow's pouch method. Both methods turned out to be successful and simpler than the original Pawlow's pouch method. In both methods, the miniature rumens were formed on the junction of 2 veins; vena ruminalis sinistra and vena ruminalis dextra. Both method 1 and 2 have the advantage of shortening the operation time, but caution was necessary to prevent the fistual formation between the rumen and miniature rumen in Method 1.

This paper deals with the histopathological observations of the perirenal edema in pigs and rabbits administered with Amaranthus retroflexus, based on the clinical and pathological observations of the porcine perirenal edema naturally occurring in Korea R. In the natural case, clinical signs are trembling, weakness and incoordination of the hindquarters, followed by sternal recumbency, coma and death. Death usually occurs within 24 hrs after the appearance of illness signs

Cells with an epithelioid morphology were isolated from the rabbit myometrium and were grown in culture. The cells had a doubling time of 53 hrs when grown in the presence of 10% fetal calf serum in Basal Eagle's medium with 3mM glutamine. In the presence of estrogen plus insulin, doubling time was reduced to 40 hrs. Creatine kinase activity upon reaching confluency was determined to be 0.019 uit per mg protein. Approximately 30% of the activity was extractable only in high ionic strength buffer. Cells also contained plasminogen activator with a specific activity of 140 CTA units per million cells

An experiment was conducted to measure the cellular immune response in guinea pigs by sensitizing the animals with 2,4-dinitrochlorobenzene (DNCB). The guinea pigs could be sensitizing with one application of DNCB. The sensitizing and challenge dose was standardized. The histological response was characteristic of a delayed hypersensitivity reaction

To investigate the factors influencing the artificial transformation in Escherichia coli, E. coli C 600 was transformed by pBR322 DNA with tetracycline and ampicillin resistant gene purified by CsCL-EtBr equilibrium density gradient centrifugation from E. coli HB 101. The best conditions for the transformation of E. coli C 600 with pBR322 DNA were treatment with 100mM CaCl2, ice incubation at 0 deg C for 45 minutes, heat shock at 42 deg C for 4 min., 30 min. of ice incubation and incubation at 37 deg C for 100 min. for gene expression in that order

In the evaluation of anthelmintic efficacy by fecal egg counts for cattle naturally infected with helminths, the reasonable technique of fecal egg counts and the reliable guidelines were determined. Modified technique of Harashigeru and Kim's sedimental tube was the most reasonable in fluke egg counts. Universal egg counting technique was preciser than McMaster egg counting technique and was lower in coefficient variation. Fecal egg counts of pretreatment should be carried out twice and mean of the egg should be calculated

The protective effects of high levels of dietary iron, copper, zinc or protein on lead toxicity were studied in chicks. Growth retardation, reduction of feed intake, anemia and accumulation of lead in the bone and kidney were observed in chicks fed a diet containing 500mg lead as chloride/kg of feed for 42 days. Early changes due to ingested lead were inhibition of red blood cell 8-aminolevulinic acid dehydrase at all doses and no effect of iron, copper, zinc or protein addition were observed

An ultrastructural study was to investigate on 2 cases of canine leiomyosarcoma. The nuclei of most tumor cells showed bilobate appearance. Most cells of the tumors had no distinct basement membrane. Most cells of the tumors had a degenerative rough endoplasmic reticulum. Intercellular matrix contained some amounts of collagen. Attachment areas between tumor cells were not apparent, but a few tight junction were clearly seen. Myofibrils were present in bipolar end area

To investigate the sensitivity of repeat tuberculin test with bovine PPD, seibert's fraction A(SFA) and avian PPD tuberculins in visible and non-visible lesion reactor cattle to HCSM tuberculin. The comparative test, under the condition of this experiment, was entirely unsatisfactory to differentiate between visible and non-visible lesion cases, the high frequency distribution of the skin fold thickness greater than 10mm to SFA tuberculin appeared in visible lesion cases, whereas the skin reaction 1 less than 4mm to the SFA tuberculin appeared in NVL cases

This study was to investigate the development pattern of epididymis in accordance with the growth of meat-type cockerels. Histological features of various ductules in epididymis of the cockerel on the age of weeks were within 10 weeks after hatching rete testis and connecting ductules were well developed but efferent ductules were observed in immature form. During 10th to 20th week, the lining epithelium of various ductules in epididymis was in the developing stage near to the mature form