The present study was undertaken to understand the present status of adoption of TANUVAS SMART mineral mixture, viz, complete adoption, partial adoption, discontinuance and rejection and its perceived attributes, namely, relative advantage, compatibility, complexity, observability and trialability using the schedule developed for the purpose and the economic impact. Partial budgeting was done to assess the economic impact of adopting TANUVAS SMART mineral mixture. Personal cosmopolite and personal localite communication channels had contributed majorly to the awareness of the dairy farmers followed by mass media. Majority of the dairy farmers (78%) discontinued the adoption of the innovation. Sixty six per cent of them had medium level of adoption behaviour followed by high (28%) and low (6%) level of adoption behavior. The reasons attributable were that the technology was perceived relatively less advantageous and not observable. However, it was perceived as highly compatible, less complex and trialable. Net income of the dairy farmers had increased by Rs. 2,216.80 per dairy animal per lactation due to the adoption of TANUVAS SMART Mineral Mixture. The status of adoption of TANUVAS SMART Mineral Mixture, its adoption behaviour and change in net income were found to be significantly associated with land holding, area under fodder cultivation, dairy animal possession, extension agency contact and mass media exposure of the dairy farmers.

The study was conducted to document the incidence and nature of oestrus like signs and follicular biometry during the lunar and peri-lunar days in crossbred cattle. Crossbred cattle (n = 678) which were reported to be with oestrus like signs on lunar days (full moon day and new moon day) and peri-lunar days were subjected for the investigation. Only 60.6 per cent of the animals during the study period were in actual oestrus. The remaining animals (39.4%) were found to be in various reproductive statuses viz., pregnancy (17.7%), mid-cycle oestrum (6.9%) and without any oestrus characteristics (14.8 %). The diameter of the dominant follicle in cattle with oestrus signs during lunar days (10.4 ± 0.2 mm) was significantly (P < 0.05) smaller than cattle in actual oestrus during normal days (12.2 ± 0.5 mm). Exhibition of oestrous signs with smaller follicular diameter indicated the probable chances of lunar influence on the follicular dynamics, steroidogenesis and reproductive characters in cattle. 

Uterine prolapse in cats of twelve clinical cases brought to the Madras Veterinary College Teaching Hospital with a history of cats queened recently, severe straining, anorexia, pyrexia and round red swollen edematous mass protruding through the vulva were used for the present study. Based on the clinical examination, the cases were diagnosed as uterine prolapse either unilateral or bilateral horns. Ovariohysterectomy (n=6) was performed in six delayed cases and manual reduction (n=6) was done in six fresh cases. Cats that underwent surgery recovered uneventfully after one week of postoperative management. Manually reduced cases also recovered after one week of medical management. 

Birds are organisms that can be found in a variety of habitats. One of the bird habitats is paddy fields. Birds found in paddy fields generally carry out activities such as foraging, breeding, and competing. This study aims to determine the diversity of birds in paddy field habitats during the paddy ripening phase (Oryza sativa L.). The study was conducted from April to June 2022. The location of the study was Jurong Peujera Village, Ingin Jaya District, Aceh Besar Regency. This study used the Point Count method with a radius of 50 m and a distance between points of 150 m. Observations were made at nine observation points with four repetitions, in which the duration of observations was 10 minutes at each observation point. The observation was conducted in the morning and evening at 06.30-10.00 AM and 03.30-05.00 PM. The results obtained were 2586 bird individuals of 14 species from 11 families. The species with the highest number of individuals areLonchura punctulata, with 1535 individuals, andBubulcus ibis, with 637 individuals.Ardeidae(22%) andEstrildidae(15%) are the highest percentage of families by species. The Shannon-Wiener bird species diversity index in the paddy field area during the paddy ripening phase was obtained H'=1.24, indicating the bird species' diversity level is moderate. Meanwhile, based on the dominance index, Simpson shows a value of C=0.42, which indicates a low bird dominance level. The low level of bird dominance is due to the moderate species diversity in the paddy field area.

Blood is a good growth medium for bacteria by preventing the accumulation of toxic oxygen (peroxide and superoxide) and increasing the growth ability of these bacteria. The purpose of this study was to evaluate the ability of lysed sheep blood and lysed cow blood as a substitute for lysed horse blood on Campylobacter jejuni growth media, as well as evaluate the growth of C. jejuni from chicken meat samples on culture media supplemented with lysed sheep blood and lysed cow blood compared to the media cultures using lysed horse blood supplements. The concentration of lysed sheep blood, lysed cow blood, and lysed horse blood used was 5% each. The chicken meat samples used in this study were 30 samples. Based on the results, 14 samples of C. jejuni bacteria showed the ability to grow up to 107 and 16 samples up to 106. In the media added with lysed cow blood supplement only one sample was able to grow up to 106, 17 samples grew up to 105, and 12 samples grew up to 104. These results statistically used the one-way analysis of variance (ANOVA) test indicating a significant difference significant (P0.05). Further tests using post hoc least significance different (LSD) showed a comparison of the average growth in lysed horse blood, lysed sheep blood, and lysed cow blood significantly different (P 0.05), while the average value of each treatment was the addition of lysed horse blood supplements, lysed sheep blood, and cow blood consecutive lysis was 8.551 0.340, 7.117 0.544, and 5.265 0.548. The addition of lysed sheep blood and lysed cow blood to bolton broth media in this study can be used for C. jejuni isolation. However, the blood of lysed sheep had a higher effectiveness in the ability to grow C. jejuni, which was close to the ability to grow with the addition of lysed horse blood as a control, compared to the addition of lysed cow blood.

A cross-sectional study was conducted from December, 2019 to May, 2020 with the aim of determining seroprevalence and identifying the potential risk factors of brucellosis in dairy cows with recent cases of abortion and their owners and farm workers in selected sites in the central highland of Oromia, Ethiopia. The overall seroprevalence of bovine and human brucellosis was 0.61% (95%CI: 0.0162.09) and 1.21% (95%CI: 0.0324.27) using combined RBPT and CFT tests, respectively. The late stage of abortion (OR = 14.74, p =0.0002), retained fetal membrane (OR = 32.74, p=0.006), market-based stock replacement (OR = 16.55, p=0.002), and presence of parturition pen (OR = 11.511, p=0.027) were found to be significantly associated with seropositivity for Brucella infection in dairy cattle. Human housing (OR = 1.8, p = 0.002), contact with aborted fetus (OR = 21.19, p = 0.017), and drinking raw milk from aborted (OR = 5.72, 0.019) and retained fetal membrane (OR = 4.22, p= 0.029) cows all had a significant influence on human brucellosis seropositivity. A structured interview question was administered to 284 respondents. Accordingly, most respondents had no knowledge of bovine brucellosis and their zoonotic effects (93.3%) in contrast most of them drink raw milk. Implementation of a test and slaughter strategy with compensation is recommended with this low prevalence. In the case of human brucellosis, implementing one health approach framework should be practiced.

This study aims to test the samples of abdominal fluid from cats suspected of having FIP by using vinegar as a substitute for acetic acid in the rival method. Feline Infectious Peritonitis (FIP) is a viral disease that is not zoonotic, but is very fatal with an infectious prognosis in various types of cats and its transmitted orally. This study aims to determine the positive or negative results of FIP infection in cats. The diagnosis of FIP can be seen through clinical symptoms such as lack of appetite, diarrhea, weakness, dehydration (reaching 8%), Body Condition Score (BCS) 2, enlarged abdomen, and ultrasound results showing ascites and swollen kidneys. This study uses vinegar as a substitute for acetic acid in the rival method. The test was carried out experimentally in a laboratory. The research data were analyzed descriptively, the results of a positive FIP reaction were indicated by the presence of a cloudy jellyfish-shaped precipitate in the test tube.

Bovine trypanosomiasis is a parasitic disease caused by protozoans of the genus Trypanosoma. The disease cause economic losses in livestock production. In order to determine the status of research on this disease in Côte d’Ivoire, we used the systematic review method and meta-analysis. Three electronics databases, namely Google Scholar, PubMed and CrossRef were used to search for publications on trypanosomiasis prevalence that met our inclusion criteria. Twenty five articles were identified, 11 of which met the inclusion criteria. Bovine trypanosomiasis prevalence of 2.99% (95% confidence interval [CI]: 2.96% – 3.01%) to 25.28% (95% CI: 25.17% – 25.38%) were recorded between 1960 and 2021. The analyses showed that the most infected regions were the Bagoue 11.26% (95% CI: 11.25% – 11.27%), Bounkani 14.94% (95% CI: 14.93% – 14.95%), Gbeke 10.34% (95% CI: 10.33% – 10.35%), Marahoue 13.79% (95% CI: 13.78% – 13.80%), Poro 8.50% (95% CI: 8.49% – 8.51%), and Tchologo 11.83% (95% CI: 11.82% – 11.84%).The most sensitive diagnostic method used was the polymerase chain reaction (PCR). The species of trypanosomes diagnosed were Typanosoma vivax 4.99% (95% CI: 4.97% – 5.01%), T. congolense 1.51% (95% CI: 1.49% – 1.52%), and T. brucei 0.61% (95% CI: 0.59% – 0.62%). Despite some variation, the prevalence of bovine trypanosomiasis in Côte d’Ivoire caused mainly by T. vivax has increased in the years between 1977 and 2017. Efforts to control tsetse and other mechanical vectors should also be put in place to minimize its transmission. Contribution: The authors studied the prevalence of bovine trypanosomiasis using the systematic review method and MA in order to determine the status of research on this disease in Côte d’Ivoire.

Stroke is a major cause of death and long-term disability. Chlorogenic acid is a phenolic compound with a potent neuroprotective effect. γ-enolase is a phosphopyruvate hydratase found in mature neurons and plays an important role in neuronal survival. This study investigated whether chlorogenic acid regulates the expression of γ-enolase during cerebral ischemia. Middle cerebral artery occlusion (MCAO) was performed to induce cerebral ischemia. Adult male rats were used and chlorogenic acid (30 mg/kg) or phosphate buffered saline (PBS) was injected intraperitoneally 2 hours after MCAO surgery. Cerebral cortical tissues were collected 24 hours after MCAO surgery. Our proteomic approach identified the reduction of γ-enolase caused by MCAO damage and the mitigation of this reduction by chlorogenic acid treatment. Results of reverse transcription-polymerase chain reaction and Western blot analyses showed a decrease in γ-enolase expression in the PBS-treated MCAO group. However, chlorogenic acid treatment attenuated this decrease. Results of immunofluorescence staining showed the change of γ-enolase by chlorogenic acid treatment. These results demonstrated that chlorogenic acid regulates the γ-enolase expression during MCAO-induced ischemia. Therefore, we suggest that chlorogenic acid mediates the neuroprotective function by regulating the γ-enolase expression in cerebral ischemia and may be used as a therapeutic agent for brain diseases including stroke.

Two 1-year-old Korean native steers in the same herd presented severe hemorrhagic diarrhea. Case 1 had severe dehydration and died after 3 days, whereas case 2 had anorexia, depression, and severe diarrhea with mucus and blood. Only case 2 was necropsied, and bovine viral diarrhea virus-2a (BVDV2a) was detected in the tissues of its alimentary tract. Gross lesions, including erosion, ulceration, and extensive hemorrhage, were observed in the digestive tract mucosa. Immunohistochemistry revealed marked positive staining for BVDV2a antigen in the large intestine. These findings are indicative of hemorrhagic disease caused by BVDV2a in a native Korean steer.