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Enterovirus E infects bovine peripheral blood mononuclear cells. Implications for pathogenesis?
2023
Małaczewska Joanna | Wróbel Małgorzata | Kaczorek-Łukowska Edyta | Rękawek Wojciech
Enterovirus E (EV-E) is a common viral pathogen endemic in cattle worldwide. Little is known, however, about its potential interactions with bovine immune cells.
Показать больше [+] Меньше [-]Antimicrobial resistance of Listeria monocytogenes serogroups IIa and IVb from food and food-production environments in Poland
2023
Lachtara Beata | Wieczorek Kinga | Osek Jacek
Listeria monocytogenes is an important foodborne pathogen responsible for human listeriosis, which is a disease with high hospitalisation and mortality rates. The bacteria are usually susceptible to most antibacterial substances, but resistance to some of them has been recently observed. The present study introduces the evidence on the emergence of antibiotic resistance among L. monocytogenes strains isolated from food and food-production environments in Poland.
Показать больше [+] Меньше [-]Distribution of carp edema virus in organs of infected juvenile common carp
2023
Matras Marek | Stachnik Magdalena | Borzym Ewa | Maj-Paluch Joanna | Reichert Michał
The disease caused by carp edema virus (CEV) manifests with lethargy as a primary sign; this observation in koi in Japan gained the disease the name koi sleepy disease (KSD). In the years following the discovery of the virus in Japan, KSD cases have been noted in the UK in koi and common carp. Conducting research in order to expand knowledge of the processes of distribution of CEV in infected fish organs will be helpful for eradication and diagnostic purposes.
Показать больше [+] Меньше [-]An initial characterisation of the Unfolded Protein Response pathway in haematopoietic canine cancer cell lines – a necessary step for the future development of new therapies in dogs with neoplasia
2023
Hernández-Suárez Beatriz | Gillespie David A. | Obmińska-Mrukowicz Bożena | Pawlak Aleksandra
New and more effective therapies for canine cancer patients are urgently required and this necessitates advanced experimental research. Dogs are good models for studies in comparative oncology; however, canine cancer cell biology research is currently limited by low availability of validated antibody reagents and techniques. This study characterises the expression of key components of the unfolded protein response (UPR) in a panel of haematopoietic canine cancer cell lines using commercially available antibodies, and validates the methods used to study this pathway.
Показать больше [+] Меньше [-]Inter-laboratory validation of liquid chromatography–tandem mass spectrometry multi-mycotoxin determination in animal feed – method transfer from the reference laboratory to regional laboratories
2023
Jedziniak Piotr | Panasiuk Łukasz | Pietruszka Katarzyna | Kolano Krzysztof | Zbytniewski Radosław
The results are presented of the inter-laboratory validation of a liquid chromatography–tandem mass spectrometry method for the determination of eight mycotoxins (aflatoxin B1, deoxynivalenol, fumonisin B1, fumonisin B2, ochratoxin A, toxin T-2, toxin HT-2 and zearalenone) in animal feeds.
Показать больше [+] Меньше [-]Incidence and the risk of occurrence of benign and malignant canine skin tumours in Poland – a five-year retrospective study
2023
Śmiech Anna | Bulak Kamila | Łopuszyński Wojciech | Puła Agata
The aim of the study was to compile data on the frequency and distribution of canine skin tumours and determine the risk of these being malignant as opposed to benign. This determination proceeded from tumour histogenesis and gave consideration to the dog’s breed, sex, age and the anatomical location of tumours.
Показать больше [+] Меньше [-]Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds
2023
Beinhauerova Monika | Slana Iva
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a chronic infectious intestinal disease occurring in domestic and wild ruminants. Early diagnosis of infected herds enabling timely adoption of control measures is tremendously important in view of the fact that the disease has a significant economic impact on farmers. The aim of this study was to evaluate the possibility of rapid detection of viable MAP on small ruminant farms based on environmental sample examination using a novel phage-based test named Actiphage.
Показать больше [+] Меньше [-]Possibilities of using mussels (Mytilus galloprovincialis) to predict rotavirus contamination in Albania
2023
Lugaj Arta | Korro Kastriot | Tolomeo Anna Maria | Bakiu Rigers | Santovito Gianfranco
Rotaviruses are non-enveloped viruses that each consist of 11 double-stranded RNA molecules. These viruses are able to persist in the environment, and therefore play a fundamental role in the epidemiology of gastroenteritis and severe diarrhoea in children worldwide. While mussels have been primarily used as indicators of chemical pollution, they can also be used to monitor viral contamination. The purpose of this study was to demonstrate that the Mytilus galloprovincialis mussel can also be used to detect microbial contamination, owing to its tendency to naturally concentrate viruses and other pathogens.
Показать больше [+] Меньше [-]Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia
2023
Widodo Agus | Lamid Mirni | Effendi Mustofa Helmi | Tyasningsih Wiwiek | Raharjo Dadik | Khairullah Aswin Rafif | Kurniawan Shendy Canadya | Yustinasari Lita Rakhma | Riwu Katty Hendriana Priscilia | Silaen Otto Sahat Martua
Escherichia coli is an opportunistic bacteria that can grow easily, produce toxins, and resist antibiotics. The phenomenon of E. coli developing multidrug resistance is currently the subject of extensive research. The objective of this study was to molecularly identify blaTEM and blaCTX-M genes in multidrug-resistant E. coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia.
Показать больше [+] Меньше [-]Can the examination of different types of hive samples be a non-invasive method for detection and quantification of viruses in honey bee (Apis mellifera L.) colonies?
2023
Čukanová Eliška | Prodělalová Jana | Palíková Miroslava | Kováčová Kristýna | Linhart Petr | Papežíková Ivana
Honey bee viruses have been shown to negatively affect the vigour and longevity of European honey bees (Apis mellifera L). In the present work, beehive materials were tested for their potential to serve as non-invasive samples for honey bee virus detection.
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