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ERRATA: Ferramenta para detecção da conexão de violência doméstica e maustratos a animais de companhia em Botucatu, São Paulo, Brasil | ERRATUM: A tool for detection of the connection between domestic violence and companion animal mistreatment in Botucatu, São Paulo, Brazil Полный текст
2024
O artigo "Ferramenta para detecção da conexão de violência doméstica e maustratos a animais de companhia em Botucatu, São Paulo, Brasil" [ Braz J Vet Res Anim Sci 2024;61:e215417, doi.org/10.11606/issn.1678-4456.bjvras.2024.215417] na primeira forma publicada ocorre uma falha na ordem dos autores. A ordem correta dos autores é: Tália Missen Tremori, Mariel Adan Everson, Juliana Santeramo, Laiza Bonela Gomes, Sérvio Túlio Jacinto Reis, Laila Massad Ribas, Mara Rita Rodrigues Massad and Noeme Sousa Rocha. | In the article "A tool for detection of the connection between domestic violence and companion animal mistreatment in Botucatu, São Paulo, Brazil" [ Braz J Vet Res Anim Sci 2024;61:e215417, doi.org/10.11606/issn.1678-4456.bjvras.2024.215417] in the first published form it happens a fail in the authors order. The correct order of the authors is: Tália Missen Tremori, Mariel Adan Everson, Juliana Santeramo, Laiza Bonela Gomes, Sérvio Túlio Jacinto Reis, Laila Massad Ribas, Mara Rita Rodrigues Massad and Noeme Sousa Rocha.
Показать больше [+] Меньше [-]Estratégias para aumentar o número de adoção de cães em um abrigo brasileiro | Strategies to increase the number of dog adoptions in a Brazilian shelter Полный текст
2024
Baldan, Ana Lucia | Garcia, Rita de Cassia Maria | Baldan, Ana Lucia | Garcia, Rita de Cassia Maria
Animais em situação de rua é uma realidade muito triste no Brasil e sabe se que o abandono é um problema multifatorial e que gera impacto negativo no bem-estar dos animais e riscos à saúde pública. Animais abandonados são acolhidos, na sua maioria, por Organizações Não Governamentais (ONG), Organizações da Sociedade Civil (OSC) espalhadas pelo país. Para mitigar a problemática do abandono essas organizações buscam a promoção de eventos de adoção para tentar sanar o número cada vez mais crescente de animais nesses locais. Mas todo evento de adoção precisa ser realizado de forma adequada e criteriosa, e o acompanhamento da nova família ou novo tutor no pós-adoção é fundamental para identificar riscos de um novo abandono. A literatura mostra que a adoção mal-sucedida está associada a problemas da interação entre tutores e cães, que podem resultar em comportamento indesejáveis. Este estudo teve como objetivo trazer e aplicar pela primeira vez no Brasil dois programas internacionais, adaptados para nossos abrigos, o Passeio para Adoção (PA) e o Lar Adotivo (LA). Os dois programas permitem que famílias pré-cadastradas possam interagir com os cães fora do abrigo, seja em passeios nos parques, lojas e restaurantes ou passando uma ou mais noites com os cães em suas casas. Essa interação fora do abrigo propicia uma aproximação melhor para o futuro adotante e o cão, aumentando a chance de adoção que é o objetivo principal desse projeto de pesquisa. A cidade brasileira escolhida para aplicar o estudo foi Curitiba, no estado do Paraná. As coletas foram realizadas de outubro de 2022 a setembro de 2023 com 22 cães participantes, 7 nos dois programas, com 17 cães do PA adotados e 9 do LA. Muitos cães foram adotados pela família voluntária, um do PA e 7 do LA, o que comprova um resultado positivo para aplicação dos programas principalmente o LA. | Stray dogs are an unfortunate reality in Brazil, and abandonment is a known multifactorial problem that generates a negative impact on animal welfare and risks to public health. Abandoned animals are primarily taken in by Non-Governmental Organizations (NGOs) and Civil Society Organizations (CSOs) across the country. To mitigate the problem, these organizations promote adoption events to remedy the increasingly growing number of abandoned animals. However, every adoption event must be appropriately and carefully carried out, and monitoring the new family or guardian post- adoption is essential to identify risks of new abandonment. The literature shows that unsuccessful adoption is associated with problems in the interaction between owners and dogs, which can result in undesirable behavior. This study aimed to bring and apply for the first time in Brazil two international programs adapted for our shelters, the Field Trip (Passeio para Adoção – PA) and the Sleepover (Lar Adotivo -LA). The two programs allow pre-registered families to interact with the dogs outside the shelter, whether through walks in parks, stores, and restaurants or by spending one or more nights with them in their homes. This interaction outside the shelter provides a better approach to the future adopter and the dog, increasing the chance of adoption, which is the main objective of this research project. The Brazilian city chosen to apply the study was the Curitiba state of Paraná. Data were collected from October 2022 to September 2023, with 22 participating dogs, 7 in both programs and 17 dogs adopted from PA and nine from LA. Many dogs were adopted by volunteer families, one from PA and 7 from LA, which proves a positive result for applying the programs, predominantly from LA.
Показать больше [+] Меньше [-]Epidemiologia do abandono: razões pelas quais tutores procuram Organizações da Sociedade Civil (OSC’s) de proteção animal para entregade seus animais domésticos | Epidemiology of Abandonment: reasons why guardians seek out animal protection Civil Society Organizations (CSOs) to give up their pets Полный текст
2024
Brugnerotto, Michele | Montiani-Ferreira, Fabiano | Escorsim, Silvana Maria | Garcia, Rita de Cassia Maria | Brugnerotto, Michele | Montiani-Ferreira, Fabiano | Escorsim, Silvana Maria | Garcia, Rita de Cassia Maria
O abandono de animais domésticos é um problema frequente nas áreas urbanas, impacta negativamente os animais, o ser humano e o meio ambiente e necessita de uma abordagem sob a estratégia de saúde única para compreender o contexto em que este problema está inserido, possibilitando a construção de medidas para o seu enfrentamento. O objetivo deste trabalho foi identificar os principais motivos relatados por tutores que entraram em contato com Organizações da Sociedade Civil (OSC’s) de proteção animal para realizar a entrega de seu animal doméstico. Verificou-se que a maioria dos animais eram filhotes (34,9%, 44/126), não tinham uma raça definida (54,8%, 69/126), não eram castrados (57,9%, 73/126), apresentavam tempo de convivência com o tutor menor que 6 meses (54%, 68/126) e foram resgatados da rua (42,9%, 54/126). Os motivos mais relatados para entrega de seu animal foram questões de moradia (44,2%, 23/52), seguido de problemas financeiros (30,8%, 16/52) e doença do tutor (11,5%, 6/52). Sobre o perfil dos tutores que participaram desta pesquisa, a maioria era do gênero feminino (71,2%, 37/52), possuía ensino médio completo (46,2%, 24/52), tinha filhos (65,4%, 34/52) e não recebia nenhum benefício social (71,2%, 37/52). Quando foram oferecidas alternativas de apoio para o tutor desistir da entrega ou permanecer com o animal, 90,4% (47/52) informaram que não haveria nada que possibilitaria a permanência do animal. Este estudo é um primeiro passo para a compreensão do abandono de animais no Brasil. Com este estudo verificamos que poucas pessoas estão dispostas a solucionar a situação geradora do desejo de entrega do animal, demonstrando que o enfrentamento ao abandono vai muito além de um motivo informado, este, frequentemente, parece ter a intenção de respaldar o abandono. Compreender o comportamento humano no abandono de animais domésticos é um grande desafio, pois raramente os tutores estão dispostos a solucionar a situação, então, para propor medidas de enfrentamento será necessário desenvolver maneiras de fortalecer o vínculo humano-animal. | Abandoning domestic animals is a frequent problem in urban areas, harming animals, humans, and the environment. A single health strategy-based approach is necessary to understand the context of this problem, making it possible to build measures to tackle it. This study aimed to identify the main reasons given by guardians who contacted animal protection Civil Society Organizations (CSOs) to surrender their pets. We found that the majority of the animals were puppies (34.9%, 44/126), did not have a defined breed (54.8%, 69/126), were not neutered (57.9%, 73/126), had lived with their guardian for less than 6 months (54%, 68/126), and were street-rescued (42.9%, 54/126). The most frequently reported reasons for giving up their animals were housing issues (44.2%, 23/52), followed by financial problems (30.8%, 16/52), and the guardian’s illness (11.5%, 6/52). Regarding the profile of the guardians taking part in this study, the majority were female (71.2%, 37/52), had completed high school (46.2%, 24/52), had children (65.4%, 34/52), and did not receive any social benefits (71.2%, 37/52). When guardians were offered alternative support to give up or keep the animal, 90.4% (47/52) said nothing would enable them to keep it. This study is a first step towards understanding animal abandonment in Brazil. Furthermore, it shows that few people are willing to resolve the situation that led to them wanting to give up the animal, demonstrating that dealing with abandonment goes far beyond an informed reason, which often seems to have the intention of supporting abandonment. Understanding human behavior in abandoning domestic animals is a significant challenge, as guardians rarely resolve the situation. Thus, developing ways of strengthening the human-animal bond will be necessary to propose coping measures.
Показать больше [+] Меньше [-]Avaliando o impacto de um curso em Educação Humanitária Solucionária para professores | Evaluating the impact of a course in Solutionary Humane Education for teachers Полный текст
2024
Costa, Maria Elisabete da | Dias, Elisângela Batista Vieira Salgueiro | Stacheski, Gabriela Vitória | Brugnerotto, Michele | Garcia, Rita de Cassia Maria | Costa, Maria Elisabete da | Dias, Elisângela Batista Vieira Salgueiro | Stacheski, Gabriela Vitória | Brugnerotto, Michele | Garcia, Rita de Cassia Maria
The contemporary global context is characterized by the presence of multifaceted challenges, including climate change, armed conflicts, socio-economic disparities, food shortages and animal cruelty. Humane Education is the pillar for changing people's behavior and attitudes towards the environment, animals and society in general, promoting understanding of the challenges faced and their origins, as well as transforming them in a positive way. The aim was to train teachers in transformative humanitarian education in order to prepare them to analyze global problems in a critical, systemic, strategic and creative way. In addition, it seeks to motivate them to search for solutions that maximize well-being and minimize harm in everyday life, as well as enabling them to use tools acquired in a school environment to encourage active citizenship among their students. The Solving Humanitarian Education course inspired by the experience of the Institute for Humane Education took place in online format on the UFPR Virtual platform from 07/03/2022 to 08/05/2022 for teachers from all over the country. The course was made up of issues related to human rights, social justice, sustainability, animal protection and other interconnected themes in a broad and solution oriented way divided into 9 modules.Participants' knowledge was assessed using a pre- and post-course questionnaires in a qualitative and quantitative way and a focus group. The course received 221 pre registrations and 50 of them completed the Initial Questionnaire and were enrolled. Of the 50 teachers registered in the course, 10 completed the entire program. An increase of empathy and compassion towards non-human beings was observed, and reflected in a change in their perceptions of the sentience of various animal species. There was a rising awareness on behalf of the professionals of seeking pragmatic solutions to challenges faced by their communities. | O contexto global contemporâneo se caracteriza pela presença de desafios multifacetados, abarcando questões como as mudanças climáticas, conflitos armados, disparidades socioeconômicas, escassez de alimentos e maus-tratos contra animais. A Educação Humanitária é o pilar para a mudança de comportamento e atitude das pessoas em relação ao meio ambiente, aos animais e à sociedade em geral, promovendo compreensão dos desafios enfrentados e suas origens, bem como sua transformação de maneira positiva. Objetivou-se capacitar docentes em Educação Humanitária transformadora com a finalidade de prepará-los a analisar, de maneira crítica, sistêmica, estratégica e criativa, os problemas globais. Ademais, visa motivá-los a buscar soluções que maximizem o bem-estar e minimizem o dano na vida cotidiana, além de habilitá-los a utilizar as ferramentas adquiridas no ambiente escolar para fomentar a cidadania ativa entre seus alunos. O curso Educação Humanitária Solucionária, inspirado na experiência do Institute for Humane Education, realizou-se no formato à distância pela plataforma UFPR Virtual, no período de 07/03/2022 a 08/05/2022, para professores de todo o país. O curso foi composto por questões relacionadas aos direitos humanos, justiça social, sustentabilidade, proteção animal e outros temas interconectados de uma forma ampla e solucionária, dividido em 9 módulos. O conhecimento dos participantes foi mensurado por meio de um grupo focal e questionários, pré e pós-curso, de forma quali-quantitativa. O curso recebeu 221 pré-inscrições, das quais 50 foram efetivadas por meio do preenchimento do Questionário Inicial. Dos 50 professores matriculados no Curso, 10 concluíram todo conteúdo programático. Observouse o aumento da empatia e compaixão relacionadas aos seres não humanos, refletido na alteração de suas percepções sobre a senciência de várias espécies animais. Constatou-se um crescente interesse por parte dos profissionais em buscar soluções pragmáticas e solucionadoras para os desafios que suas comunidades enfrentam.
Показать больше [+] Меньше [-]Editorial | Editorial Полный текст
2024
Garcia, Rita de Cassia Maria | Garcia, Rita de Cassia Maria
Editorial for the special Collective Veterinary Medicine | Editorial para o especial Medicina Veterinária do Coletivo
Показать больше [+] Меньше [-]Three-minute nebulization of gentamicin in healthy dogs results in therapeutic concentrations in bronchoalveolar lavage fluid while remaining below the toxic range values in blood. Полный текст
2024
Lyssens, Aurélie | Biénès, Tom | Fastrès, Aline | Machiels, Hélène | Moreau, Camille | Tutunaru, Alexandru-Cosmin | DEVILLE, Marine | Charlier, Corinne | Clercx, Cécile | Billen, Frédéric
peer reviewed | [en] OBJECTIVE: To determine and compare the concentration of gentamicin in the lower airways and serum of healthy spontaneously breathing dogs after nebulization with 5% undiluted gentamicin during 3 versus 10 minutes. ANIMALS: 10 healthy experimental Beagles. METHODS: This was a prospective crossover study. A standardized bronchoalveolar lavage (BAL) procedure was performed in each dog after 1 week of administration of each of 2 different gentamicin nebulization protocols separated by a 1-week washout period. The 2 protocols consisted of nebulization of 5% undiluted gentamicin (50 mg/mL) twice daily either during 10 minutes per session (± 95 mg; 10-minute protocol) or 3 minutes per session (± 28 mg; 3-minute protocol). BAL fluid (BALF) was obtained under general anesthesia using a bronchoscope within 15 minutes after administration of the last nebulization. Blood was collected within 5 minutes after BALF collection. BALF and serum gentamicin concentrations were determined by particle-enhanced turbidimetric inhibition immunoassay. Concentrations between protocols were compared using a paired t test. RESULTS: Both BALF and serum gentamicin concentrations were higher after the 10-minute protocol compared with the 3-minute protocol (mean ± SD: 2.41 ± 0.87 mg/L vs 1.25 ± 0.31 mg/L, P = .001; and 1.02 ± 0.59 mg/L vs 0.31 ± 0.24 mg/L, P < .0001 in BALF and serum, respectively), while the BALF-to-serum ratio did not differ between the protocols (3.75 [1.37 to 5.75] (median [IQR]) in the 3-minute protocol vs 2.48 [2.02 to 2.67] in the 10-minute protocol; P = .754). CLINICAL RELEVANCE: A 3-minute nebulization of gentamicin seems to achieve sufficient concentrations of gentamicin in the BALF to have good efficacy against aminoglycoside-sensitive bacteria while remaining below the toxic range values in blood.
Показать больше [+] Меньше [-]Evaluation of the “3-minute search and collect” protocol for dog ectoparasite surveys in the domestic-wildlife interface area Полный текст
2024
Kamyingkird, Ketsarin | Chimnoi, Wissanuwat | Pioch, Florian | Dupont, Sonia | Krieng-Udom, Areeya | Kritiyakan, Anamika | Phimpraphai, Waraphon | de Garine-Wichatitsky, Michel | Kasetsart University [Bangkok, Thailand] (KU) | Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | Université de Montpellier (UM) | Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Evaluation of the “3-minute search and collect” protocol for dog ectoparasite surveys in the domestic-wildlife interface area Полный текст
2024
Kamyingkird, Ketsarin | Chimnoi, Wissanuwat | Pioch, Florian | Dupont, Sonia | Krieng-Udom, Areeya | Kritiyakan, Anamika | Phimpraphai, Waraphon | de Garine-Wichatitsky, Michel | Kasetsart University [Bangkok, Thailand] (KU) | Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | Université de Montpellier (UM) | Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Source Agritrop Cirad (https://agritrop.cirad.fr/612069/) | International audience | Background and Aim: Dog ectoparasites are a major concern regarding the emergence of several vector-borne zoonotic diseases associated with domestic dogs. Information on the quantified assessment of ectoparasite collection methods from dogs remains limited. Therefore, this study aimed to evaluate the effectiveness of the ”3-minute method” for col- lecting dog ectoparasites in the human and wildlife border interface in Northern Thailand. Materials and Methods: The ”3-minute method” (TMM) was compared with the bath- ing-combing method (BCM) in 31 domestic dogs in the domestic-wildlife interface area, comprising 4 villages in Nan province, Thailand, from July 2022 to July 2023. All ectopara- sites were collected, and morphological identification was confirmed. The percentage of agreement between TMM and BCM was calculated using Kappa. A seasonal comparison of ectoparasite infestation was conducted using the TMM method. Results: Comparatively, the diversity of ectoparasites collected by TMM was revealed to be similar to the BCM method: ticks (Rhipicephalus sanguineus (Ixodida: Ixodidae), Hae- maphysalis spp. (Ixodida: Ixodidae), Dermacentor spp. (Ixodida: Ixodidae)), fleas (Cten- ocephalides felis orientis (Siphonaptera: Pulicidae), Ctenocephalides felis (Siphonaptera: Pulicidae)), and lice (Heterodoxus spp. (Phthiraptera: Boopiidae)). More ectoparasites were collected by the BCM than by the TMM method. The average efficiency percentages of TMM and BCM were 12.8% and 87.2%, respectively. The observed percentage agree- ment between BCM and TMM was very good (K = 0.9) for ticks, good (K = 0.7) for fleas, and moderate (K = 0.5) for lice. The diversity of ectoparasites in dogs living in the domestic dogs-wildlife interface area showed that there were 4 species of ectoparasites collected in the dry season compared to 6 species in the rainy season. Conclusion: The fast-body search for 3 minutes is a fast, inexpensive, and effective method for the identification and study of the diversity and abundance of ectoparasites from owned dogs when compared to bathing and combing methods with Amitraz. This method can be used as a non-invasive technique to collect ectoparasites from domesti- cated dogs for further study. Sharing ectoparasites from wildlife to domestic dogs in the domestic-wildlife interface area has reported.
Показать больше [+] Меньше [-]Evaluation of the “3-minute search and collect” protocol for dog ectoparasite surveys in the domestic-wildlife interface area Полный текст
2024
Kamyingkird, Ketsarin | Chimnoi, Wissanuwat | Pioch, Florian | Dupont, Sonia | Krieng-Udom, Areeya | Kritiyakan, Anamika | Phimpraphai, Waraphon | De Garine-Wichatitsky, Michel
Background and Aim: Dog ectoparasites are a major concern regarding the emergence of several vector-borne zoonotic diseases associated with domestic dogs. Information on the quantified assessment of ectoparasite collection methods from dogs remains limited. Therefore, this study aimed to evaluate the effectiveness of the ”3-minute method” for col- lecting dog ectoparasites in the human and wildlife border interface in Northern Thailand. Materials and Methods: The ”3-minute method” (TMM) was compared with the bath- ing-combing method (BCM) in 31 domestic dogs in the domestic-wildlife interface area, comprising 4 villages in Nan province, Thailand, from July 2022 to July 2023. All ectopara- sites were collected, and morphological identification was confirmed. The percentage of agreement between TMM and BCM was calculated using Kappa. A seasonal comparison of ectoparasite infestation was conducted using the TMM method. Results: Comparatively, the diversity of ectoparasites collected by TMM was revealed to be similar to the BCM method: ticks (Rhipicephalus sanguineus (Ixodida: Ixodidae), Hae- maphysalis spp. (Ixodida: Ixodidae), Dermacentor spp. (Ixodida: Ixodidae)), fleas (Cten- ocephalides felis orientis (Siphonaptera: Pulicidae), Ctenocephalides felis (Siphonaptera: Pulicidae)), and lice (Heterodoxus spp. (Phthiraptera: Boopiidae)). More ectoparasites were collected by the BCM than by the TMM method. The average efficiency percentages of TMM and BCM were 12.8% and 87.2%, respectively. The observed percentage agree- ment between BCM and TMM was very good (K = 0.9) for ticks, good (K = 0.7) for fleas, and moderate (K = 0.5) for lice. The diversity of ectoparasites in dogs living in the domestic dogs-wildlife interface area showed that there were 4 species of ectoparasites collected in the dry season compared to 6 species in the rainy season. Conclusion: The fast-body search for 3 minutes is a fast, inexpensive, and effective method for the identification and study of the diversity and abundance of ectoparasites from owned dogs when compared to bathing and combing methods with Amitraz. This method can be used as a non-invasive technique to collect ectoparasites from domesti- cated dogs for further study. Sharing ectoparasites from wildlife to domestic dogs in the domestic-wildlife interface area has reported.
Показать больше [+] Меньше [-]A potential antifungal bioproduct for Microsporum canis: Bee venom Полный текст
2024
Ütük, Armağan E. | Güven Gökmen, Tülin | Yazgan, Hatice | Eşki, Funda | Turut, Nevin | Karahan, Şifa | Kıvrak, İbrahim | Sevin, Sedat | Sezer, Osman | This research was financially supported by Adana Çukurova University Scientific Research Projects Department with TAY-2022-14855 with the “Investigation of the antimicrobial effects of Bee Venom ". The authors thank the Scientific Research Projects Depart
A potential antifungal bioproduct for Microsporum canis: Bee venom Полный текст
2024
Ütük, Armağan E. | Güven Gökmen, Tülin | Yazgan, Hatice | Eşki, Funda | Turut, Nevin | Karahan, Şifa | Kıvrak, İbrahim | Sevin, Sedat | Sezer, Osman | This research was financially supported by Adana Çukurova University Scientific Research Projects Department with TAY-2022-14855 with the “Investigation of the antimicrobial effects of Bee Venom ". The authors thank the Scientific Research Projects Depart
Natural treatment options for Microsporum canis dermatophytosis are being explored because of resistance to several antifungal medications. In this study, the potential antifungal effect of bee venom (BV), a natural antimicrobial agent, on M. canis was investigated. The antifungal effects of BV, fluconazole, itraconazole, amphotericin B and terbinafine were evaluated by the macrodilution method at various concentrations by modifying the microdilution method recommended by the European Committee on Antimicrobial Susceptibility Testing. All isolates were observed to be susceptible to terbinafine and fully resistant to fluconazole and amphotericin B. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of M. canis isolate 2 (Mc2) were determined as 8 µg/mL for itraconazole. The MIC and MFC values of BV were found to be 320 µg/mL for the Mc2 isolate and 640 µg/mL for the Mc6 isolate. The results showed that the isolates obtained from clinical samples in this study were highly resistant to all antifungal agents, except terbinafine. The increase in resistance indicates that antifungal drugs will become insufficient and ineffective over time and natural products such as BV should be evaluated as alternatives.Contribution: Although there are many drugs for the treatment of M. canis, the increase in resistance to antifungal agents reveals the need for the identification and development of new natural agents. Bee venom, which has been shown to have a safe and weak allergenic effect in various studies, can be tested for usability as a local antifungal drug when supported by in vivo studies.
Показать больше [+] Меньше [-]A potential antifungal bioproduct for Microsporum canis: Bee venom Полный текст
2024
Armağan E. Ütük | Tülin Güven Gökmen | Hatice Yazgan | Funda Eşki | Nevin Turut | Şifa Karahan | İbrahim Kıvrak | Sedat Sevin | Osman Sezer
Natural treatment options for Microsporum canis dermatophytosis are being explored because of resistance to several antifungal medications. In this study, the potential antifungal effect of bee venom (BV), a natural antimicrobial agent, on M. canis was investigated. The antifungal effects of BV, fluconazole, itraconazole, amphotericin B and terbinafine were evaluated by the macrodilution method at various concentrations by modifying the microdilution method recommended by the European Committee on Antimicrobial Susceptibility Testing. All isolates were observed to be susceptible to terbinafine and fully resistant to fluconazole and amphotericin B. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of M. canis isolate 2 (Mc2) were determined as 8 µg/mL for itraconazole. The MIC and MFC values of BV were found to be 320 µg/mL for the Mc2 isolate and 640 µg/mL for the Mc6 isolate. The results showed that the isolates obtained from clinical samples in this study were highly resistant to all antifungal agents, except terbinafine. The increase in resistance indicates that antifungal drugs will become insufficient and ineffective over time and natural products such as BV should be evaluated as alternatives. Contribution: Although there are many drugs for the treatment of M. canis, the increase in resistance to antifungal agents reveals the need for the identification and development of new natural agents. Bee venom, which has been shown to have a safe and weak allergenic effect in various studies, can be tested for usability as a local antifungal drug when supported by in vivo studies.
Показать больше [+] Меньше [-]Evaluation of commercial ELISA kits’ diagnostic specificity for FAST diseases in wild animals Полный текст
2024
Milićević, Vesna | Glišić, Dimitrije | Veljović, Ljubiša | Mirčeta, Jovan | Kureljušić, Branislav | Đorđević, Milutin | Vasković, Nikola | European Commission for Foot and Mouth Disease - EuFMD
Evaluation of commercial ELISA kits’ diagnostic specificity for FAST diseases in wild animals Полный текст
2024
Milićević, Vesna | Glišić, Dimitrije | Veljović, Ljubiša | Mirčeta, Jovan | Kureljušić, Branislav | Đorđević, Milutin | Vasković, Nikola | European Commission for Foot and Mouth Disease - EuFMD
Wild animals, sharing pathogens with domestic animals, play a crucial role in the epidemiology of infectious diseases. Sampling from wild animals poses significant challenges, yet it is vital for inclusion in disease surveillance and monitoring programmes. Often, mass surveillance involves serological screenings using enzyme-linked immunosorbent assay (ELISA) tests, typically validated only for domestic animals. This study assessed the diagnostic specificity of commercially available ELISA tests on 342 wild ruminant serum samples and 100 from wild boars. We evaluated three tests for foot-and-mouth disease: two for Peste des petits ruminants, two for Rift Valley fever and one for Capripox virus. Diagnostic specificity was calculated using the formula True Negative/(False Positive + True Negative). Cohen’s kappa coefficient measured agreement between tests. Results showed high specificity and agreement across all tests. Specificity for foot-and-mouth disease (FMD) ranged from 93.89% for Prionics to 100% for IDEXX, with IDvet showing 99.6%. The highest agreement was between FMD IDvet and IDEXX at 97.1%. Rift Valley fever (RVF) tests, Ingezim and IDvet, achieved specificities of 100% and 98.83%, respectively. The optimal specificity was attained by retesting single reactors and inactivating the complement.Contribution: Commercially available ELISA kits are specific for foot-and-mouth disease and similar transboundary animal diseases and can be used for highly specific wild animal testing.
Показать больше [+] Меньше [-]Evaluation of commercial ELISA kits’ diagnostic specificity for FAST diseases in wild animals Полный текст
2024
Vesna Milićević | Dimitrije Glišić | Ljubiša Veljović | Jovan Mirčeta | Branislav Kureljušić | Milutin Đorđević | Nikola Vasković
Wild animals, sharing pathogens with domestic animals, play a crucial role in the epidemiology of infectious diseases. Sampling from wild animals poses significant challenges, yet it is vital for inclusion in disease surveillance and monitoring programmes. Often, mass surveillance involves serological screenings using enzyme-linked immunosorbent assay (ELISA) tests, typically validated only for domestic animals. This study assessed the diagnostic specificity of commercially available ELISA tests on 342 wild ruminant serum samples and 100 from wild boars. We evaluated three tests for foot-and-mouth disease: two for Peste des petits ruminants, two for Rift Valley fever and one for Capripox virus. Diagnostic specificity was calculated using the formula True Negative/(False Positive + True Negative). Cohen’s kappa coefficient measured agreement between tests. Results showed high specificity and agreement across all tests. Specificity for foot-and-mouth disease (FMD) ranged from 93.89% for Prionics to 100% for IDEXX, with IDvet showing 99.6%. The highest agreement was between FMD IDvet and IDEXX at 97.1%. Rift Valley fever (RVF) tests, Ingezim and IDvet, achieved specificities of 100% and 98.83%, respectively. The optimal specificity was attained by retesting single reactors and inactivating the complement. Contribution: Commercially available ELISA kits are specific for foot-and-mouth disease and similar transboundary animal diseases and can be used for highly specific wild animal testing.
Показать больше [+] Меньше [-]Coinfection of some respiratory viruses in cattle: An abattoir study Полный текст
2024
Saeed, Intisar K. | Ali, Yahia H. | Abdellatif, Muaz Magzoub | Mustafa, Alaa | Abdel-Mageed, Ahmad M.
Coinfection of some respiratory viruses in cattle: An abattoir study Полный текст
2024
Saeed, Intisar K. | Ali, Yahia H. | Abdellatif, Muaz Magzoub | Mustafa, Alaa | Abdel-Mageed, Ahmad M.
To investigate the existence of bovine viral diarrhoea virus (BVDV), parainfluenza virus 3 (PIV-3) and respiratory syncytial virus (RSV) as well as its coinfections, a total of 420 pneumonic lung tissue samples were collected from slaughterhouses in three different areas. Samples were examined for the three viruses using antigen detection enzyme-linked immunosorbent assay (ELISA) test, and positive results were further confirmed using fluorescent antibody test and polymerase chain reaction. Prevalences detected were 10.47% for BVDV, 11.9% for parainfluenza virus type (PIV-3) and 7.6% for respiratory syncytial virus (RSV). Observed coinfections were bovine viral diarrhoea (BVD) and PIV-3 in 8.1%, BVD and RSV in only two samples (1%). Existence of all three viruses was detected in two samples (1%).Contribution: In this study, infection of the three viruses was confirmed in cattle and existence of its coinfection is documented for the first time.
Показать больше [+] Меньше [-]Coinfection of some respiratory viruses in cattle: An abattoir study Полный текст
2024
Intisar K. Saeed | Yahia H. Ali | Muaz Magzoub Abdellatif | Alaa Mustafa | Ahmad M. Abdel-Mageed
To investigate the existence of bovine viral diarrhoea virus (BVDV), parainfluenza virus 3 (PIV-3) and respiratory syncytial virus (RSV) as well as its coinfections, a total of 420 pneumonic lung tissue samples were collected from slaughterhouses in three different areas. Samples were examined for the three viruses using antigen detection enzyme-linked immunosorbent assay (ELISA) test, and positive results were further confirmed using fluorescent antibody test and polymerase chain reaction. Prevalences detected were 10.47% for BVDV, 11.9% for parainfluenza virus type (PIV-3) and 7.6% for respiratory syncytial virus (RSV). Observed coinfections were bovine viral diarrhoea (BVD) and PIV-3 in 8.1%, BVD and RSV in only two samples (1%). Existence of all three viruses was detected in two samples (1%). Contribution: In this study, infection of the three viruses was confirmed in cattle and existence of its coinfection is documented for the first time.
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