This retrospective study was done to characterize the levels of vascular endothelial growth factor (VEGF) and hypoxia inducible factor 1 (HIF-1α) in dog brains with neo-vascularization in the cerebral cortex of frontal, temporal, and parietal lobe by using immunohistochemistry (IHC) and Western blot. In neo-vascularized (NV) brains, we analyzed the number and area of blood vessels and the expression of VEGF and HIF-1α. The IHC results showed that the number and area of blood vessels, as assessed by immunolabeling for von Willebrand factor, was higher in the NV brain than in the control brain. The Western blot results showed that the level of VEGF was increased, predominantly in NV brain of the cerebral cortex relative to the clinically normal cerebral cortex, whereas the expression of HIF-1α in NV brains was not different from the control brains. Our study showed that dilatation of vessels and development of new vessels in the cerebral cortex were observed in cases of canine CNS disease and found increased expression of VEGF in canine brains with neo-vascularization.

In a crossover study, 5 calves were made acidotic by intermittent intravenous infusion of isotonic hydrochloric acid (HCl) over approximately 24 h. This was followed by rapid (4 h) or slow (24 h) correction of blood pH with isotonic sodium bicarbonate (NaHCO3) to determine if rapid correction of acidemia produced paradoxical cerebrospinal fluid (CSF) acidosis. Infusion of HCl produced a marked metabolic acidosis with respiratory compensation. Venous blood pH (mean ± Sx) was 7.362 ± 0.021 and 7.116 ± 0.032, partial pressure of carbon dioxide (Pco2, torr) 48.8 ± 1.3 and 34.8 ± 1.4, and bicarbonate (mmol/L), 27.2 ± 1.27 and 11 ± 0.96; CSF pH was 7.344 ± 0.031 and 7.240 ± 0.039, Pco2 42.8 ± 2.9 and 34.5 ± 1.4, and bicarbonate 23.5 ± 0.91 and 14.2 ± 1.09 for the period before the infusion of hydrochloric acid and immediately before the start of sodium bicarbonate correction, respectively. In calves treated with rapid infusion of sodium bicarbonate, correction of venous acidemia was significantly more rapid and increases in Pco2 and bicarbonate in CSF were also more rapid. However, there was no significant difference in CSF pH. After 4 h of correction, CSF pH was 7.238 ± 0.040 and 7.256 ± 0.050, Pco2 44.4 ± 2.2 and 34.2 ± 2.1, and bicarbonate 17.8 ± 1.02 and 14.6 ± 1.4 for rapid and slow correction, respectively. Under the conditions of this experiment, rapid correction of acidemia did not provoke paradoxical CSF acidosis.

Objective: To estimate the prevalence of perinuclear antineutrophilic cytoplasmic autoantibodies (pANCA) in the serum of healthy Soft Coated Wheaten Terriers (SCWTs) in the United Kingdom and to identify potential risk factors and heritability patterns associated with a positive result for pANCA. Animals: 188 SCWTs (age range, 18 months to 14.3 years). Procedures: Blood samples were obtained from SCWTs in various locations in England. Serum was tested for pANCA by use of an immunofluorescence assay, and total protein and albumin concentrations were determined. Pedigrees were evaluated to identify close relatives that had protein-losing enteropathy (PLE) or protein-losing nephropathy (PLN). Results: 39 of 188 (20.7%) dogs, including young dogs, had positive results for pANCA. Dogs had significantly higher odds of having positive results for pANCA if they had at least 1 littermate that had PLE or PLN (odds ratio, 12.1) or if they had at least 1 full sibling from another litter known to be affected with PLE or PLN (odds ratio, 4.0). Conclusions and Clinical Relevance: This study revealed a high prevalence of pANCA in the serum of a representative sample of healthy SCWTs in the United Kingdom and a significant association between positive results for pANCA and a diagnosis of PLE or PLN in a sibling.

Objective: To evaluate the use of retrospectively ECG-gated, contrast-enhanced, multi-detector row computed tomography (MDCT) for assessment of left ventricular function in dogs and to compare the results with those obtained by use of 2-D and M-mode echocardiographc techniques. Animals: 10 healthy Beagles. Procedures: Dogs underwent MDCT (performed by use of a 64-detector row CT system) and echocardiography under general anesthesia. Left ventricular end-systolic volume (ESV), end-diastolic volume (EDV), and ejection fraction (EF) were determined in MDCT-generated multiplanar reformatted images by use of Simpson and biplane area-length calculation methods. Results were compared with left ventricular ESV, EDV, and EF determined in echocardiographc images by use of Teichholz and bullet method calculations. Results were evaluated via Deming regression analysis and Pearson correlation tests. Bland-Altman analysis was used to assess limits of agreement and systematic errors between the 2 methods. Results: Mean values for EDV and ESV determined by use of MDCT were highly correlated with those determined by use of echocardiography, regardless of the calculation methods compared (r = 0.91 to 0.96); volumes determined by use of MDCT appeared to be higher than those determined by use of echocardiography, although most differences were nonsignificant. Mean EF determined by use of MDCT with the Simpson calculation method was highly correlated with that determined by use of echocardiography with bullet method calculations (r = 0.90). Conclusions and Clinical Relevance: Results suggested that assessment of left ventricular volume and function in dogs is feasible with MDCT. To estimate left ventricular EF with MDCT. use of the Simpson calculation method is advised.

Parasitic helminth infections in small ruminants are prevalent in South East Asia (SEA), limiting productivity and causing major economic loss for farmers. The hot, wet, tropical climate all year
round favours trichostrongylid infections, predominantly haemonchosis in sheep and goats. Commercial large scale farms, with
more than 300 animals, as well as small holders or backyard farmers with less than 50 animals face the debilitating effects of haemonchosis when they graze their animals as effective worm control is often hampered by anthelmintic resistance. In Malaysia, frequent and indiscriminate use of anthelmintics in the past has resulted
in the majority of the small ruminant population facing resistance to one or more anthelmintics. Several alternative methods of worm control are being employed by farmers; the most important and effective being cut and carry or zero grazing, where the animals are kept in pens and grass is cut and fed. In Cambodia and Myanmar,
ruminants are still tethered or stall fed with minimal drug use. In Indonesia and Thailand, commercial goat and sheep farms are fast expanding to produce breeder stock for the SEA market. However, up to
75% of the small ruminant population is still traditionally managed by small scale farmers. In most of SEA, the McMaster method for faecal worm egg counts is the only diagnostic test used to assess helminthosis in ruminants. There is an urgent need to increase awareness and information on the need for testing faecal samples regularly before drenching, conducting faecal egg count reduction tests on a yearly basis, use of the FAMACHA technique to enable
selective treatment of individual animals. The use of alternative worm control methods to manage helminthosis will help promote effective ruminant production with reduced drug use and ncourage “green” farming methods. Extension of research on local bioactive plants which may have the potential to control helminthosis may also be beneficial in the longer term.

A beef feedlot production decision support system (DSS) was developed based on Microsoft® Excel. The DSS comprises of three modules i) an ingredient database ii) a least-cost ration formulation module and iii) beef growth simulation module. The program uses empirical equations developed for tropical beef to simulate nutrient requirements and daily body weight gains based on the formulated feed ration. The formulated least cost ration can be pasted automatically into the growth model to evaluate performance and economic viability. The growth model calculates nutrient available and computes body weight gain on a daily basis, summates weight gain and stops at the targeted body weight. The data output include i) days to reach target body weight, ii) cumulative feed consumed, iii) anticipated average daily gain, iv) total cost of feed (concentrates and grass), and v) gross profit per cattle. If a portion of the feed is fed as grass, then the model also computes the pasture land required in hectares, based on the forage species
chosen. It is anticipated that the developed model can assist cattle entrepreneurs and farmers in the development of the beef cattle industry in Malaysia.

A trial and pig performance data collection was conducted to evaluate the efficiency of Luodong Bio- Fermentation Treatment Technology with zero discharge effect in a pig close house system at Tanjung Sepat, Selangor. This trial was done to gather and monitor the pig growth performance and the pig acceptance of using the Loudong Bio-fermentation Waste Treatment Technology in close house enviroment. Growth performance of the pigs were monitored by an average daily gain, feed conversion ratio, behaviour and veterinary observations. Close house temperature, moisture measurement and
bedding sampling were also conducted.

Kedah-Kelantan cattle (KK) being an indigenous breed are highly adapted to the hot-humid Malaysian climate and can survive in harsh, marginal environments. This makes the KK a valuable genetic resource, given the challenges of climate change and the changing demands of the livestock sector. Hitherto there is no comprehensive programme to genetically improve the purebred KK. Genetic improvement of the KK would be to fulfill the breeding objectives of increasing lean meat growth rate, enhancing meat quality, raising feed efficiency, improving fertility and maintaining adaptability. The breeding structure proposed is a 2-tier breeding structure, with a nucleus tier followed by a commercial tier below it. The nucleus tier would comprise of a number of pedigree farms run as a community
breeding project. A sire reference scheme is proposed, where progeny of reference sires are used as genetic links between pedigree herds and between years. Some guidelines are offered on the establishment
and implementation of the scheme. Modern breeding technology such as BLUP using an Animal Model, artificial insemination, embryo transfer, tissue scanning, MAS and MAI could be used as tools to support the KK genetic improvement programme. To address the problem of genetic erosion, emphasis should be given to the conservation and sustainable utilization of the KK. The programme is expected to have a high impact on the livestock sector. Substantial investments are needed to develop infrastructure and human capital associated with the KK breeding programme. The establishment of a KK breed society will improve awareness concerning the KK and protect the interests
of the KK breeding community.

Corona Discharge Therapy,an evidence-based therapeutic modality, was used on a 40-year-old female Malaysian elephant. The elephant was on systemic therapy for her wounds for 5 days. All therapies were then stopped for 4 days. Corona Discharge Therapy was subsequently applied on both the hind limb joints for 3 sessions. The non-invasive
treatment was made with a multi-patented, electronic therapeutic device called VET Sonotron without the concurrent use of drugs. Corona discharge beam (CDB) of the device was used to relieve the joint pain and inflammation. The skin surface of the elephant joints was scanned with the CDB emanating from a discharge electrode to
which was applied periodic bursts of radio frequency at 0.43 mHz, pulsed at 1 kHz of sound frequency. This therapy showed remarkable anti-inflammatory effects.