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Editorial Полный текст
2014
Silvio Arruda Vasconcellos | Solange Maria Gennari
Molecular detection of leptospiral carriers in sheep under tropical field conditions Полный текст
2014
Ariel Director | Gabriel Mendes de Souza Martins | Ana Paula Pereira Loureiro | Camila Hamond Regua Motta Reis | Marco Alberto Medeiros | Walter Lilenbaum
Molecular detection of leptospiral carriers in sheep under tropical field conditions Полный текст
2014
Ariel Director | Gabriel Mendes de Souza Martins | Ana Paula Pereira Loureiro | Camila Hamond Regua Motta Reis | Marco Alberto Medeiros | Walter Lilenbaum
The purpose of this study was to analyze the usefulness of PCR for the detection of leptospiral carriers in sheep under tropical field conditions. Two flocks, previously reported as seroreactive (A) and seronegative (B), were selected for this study. From those, the totality of animals of each flock, urine and vaginal fluid (VF)/semen were collected for bacteriological culture and PCR, as well as serum samples for serology. Serology confirmed the previous status of the two flocks. Culture was negative for all the samples. In PCR, animals of Flock A presented 26.7% (VF), 33.3% (semen) and 38.9% (urine) of positivity. Flock B presented 40.0% (VF), 33.3% (semen) and 5.6% (urine) of positivity by PCR. In conclusion, PCR was important to identify carriers of leptospires, including animals from a seronegative flock, what reinforces the advantages of the usage of this tool for the detection of carriers in sheep as part of control programs of leptospirosis under tropical field conditions.
Показать больше [+] Меньше [-]Molecular detection of leptospiral carriers in sheep under tropical field conditions | Detecção molecular de ovinos carreadores de Leptospira em ambiente tropical Полный текст
2014
Ariel Director | Gabriel Mendes de Souza Martins | Ana Paula Pereira Loureiro | Camila Hamond Regua Motta Reis | Marco Alberto Medeiros | Walter Lilenbaum
O objetivo do presente estudo foi analisar a aplicabilidade da PCR na detecção de ovinos carreadores de <em>Leptospira</em> em ambiente tropical. Brevemente, dois rebanhos ovinos, previamente reportados como sororeativo (A) e soronegativo (B) foram selecionados para este estudo. Da totalidade de animais de cada rebanho, amostras de urina e fluido vaginal (FV)/sêmen foram colhidas para cultura bacteriológica e PCR. Além disso, amostras de soro foram colhidas e utilizadas na sorologia (teste da soroaglutinação microscópica). Esta técnica confirmou o estado prévio dos dois rebanhos. Nenhuma amostra pura de leptospiras foi obtida no cultivo. Já na PCR, animais do Rebanho A apresentaram 26,7% (FV), 33,3% (sêmen) e 38,9% (urina) de amostras positivas. O Rebanho B apresentou 40,0% (FV), 33,3% (sêmen) e 5,6% (urina) de positividade pela PCR. Em conclusão, a PCR foi uma importante ferramenta na identificação de carreadores de leptospiras, incluindo animais do rebanho soronegativo, o que reforça as vantagens do uso desta técnica para a detecção de ovinos portadores como parte dos programas de controle da leptospirose em ambiente tropical. | The purpose of this study was to analyze the usefulness of PCR for the detection of leptospiral carriers in sheep under tropical field conditions. Two flocks, previously reported as seroreactive (A) and seronegative (B), were selected for this study. From those, the totality of animals of each flock, urine and vaginal fluid (VF)/semen were collected for bacteriological culture and PCR, as well as serum samples for serology. Serology confirmed the previous status of the two flocks. Culture was negative for all the samples. In PCR, animals of Flock A presented 26.7% (VF), 33.3% (semen) and 38.9% (urine) of positivity. Flock B presented 40.0% (VF), 33.3% (semen) and 5.6% (urine) of positivity by PCR. In conclusion, PCR was important to identify carriers of leptospires, including animals from a seronegative flock, what reinforces the advantages of the usage of this tool for the detection of carriers in sheep as part of control programs of leptospirosis under tropical field conditions.<strong></strong>
Показать больше [+] Меньше [-]Editorial Полный текст
2014
Silvio Arruda Vasconcellos | Solange Maria Gennari
Canine mammary tumors in Santos, Brazil: clinicopathological and survival profile Полный текст
2014
Luiz Roberto Biondi | Luciana Boffoni Gentile | Alexandre Aparecido Mattos da Silva Rego | Nathalia Pisciottano Noronha | Maria Lucia Zaidan Dagli
Canine mammary tumors in Santos, Brazil: clinicopathological and survival profile Полный текст
2014
Luiz Roberto Biondi | Luciana Boffoni Gentile | Alexandre Aparecido Mattos da Silva Rego | Nathalia Pisciottano Noronha | Maria Lucia Zaidan Dagli
Tumors of the mammary glands are the most common neoplasms in dogs in our country; however, there are few Brazilian reports dedicated to clinicopathological and survival studies about this disease. This report aims the clinical and pathological study of canine mammary tumors in the Santos Metropolitan Region, an area in Sao Paulo state with an estimated canine population of 120,000 animals. Data of 14,298 dogs were collected retrospectively from the medical records of the Veterinary Medical Teaching Hospital of the Metropolitan University of Santos – São Paulo – Brazil. During the study period, from records of 317 females with histopathological diagnosis of neoplasia, 170 were mammary epithelial lesions distributed in 13 benign tumors, 152 malignant (89.4% of diagnosis) and 5 non-neoplasic epithelial lesions (ductal hyperplasia). The highest prevalent malignant tumor was tubular carcinoma (38.2% of diagnosis) and Grade I tumors, corresponding to 73.0% of all diagnosis. The results have shown clinical staging of canine mammary neoplasms as an important prognostic survival factor and, in a multivariate analysis, tumor diameter, tumor grade, adjuvant chemotherapy and recurrence as covariates with predictive value for survival. Moreover, the high prevalence of tubular carcinoma qualifies the canine population ofSantosas a promising model for the translational study of this disease.
Показать больше [+] Меньше [-]Canine mammary tumors in Santos, Brazil: clinicopathological and survival profile | Neoplasias mamárias caninas em Santos, Brasil: perfil clinicopatológico e de sobrevida Полный текст
2014
Luiz Roberto Biondi | Luciana Boffoni Gentile | Alexandre Aparecido Mattos da Silva Rego | Nathalia Pisciottano Noronha | Maria Lucia Zaidan Dagli
Os tumores das glândulas mamárias são as neoplasias mais comuns em cadelas em nosso país; no entanto, são poucos os trabalhos brasileiros dedicados ao estudo clinicopatológico e de sobrevida nesta doença. O presente trabalho teve por objetivo o estudo clínico e patológico dos tumores mamários caninos na Região Metropolitana de Santos, uma área no estado de São Paulo com uma população canina estimada em 120 mil animais. Dados de 14.298 cães foram coletados retrospectivamente dos prontuários médicos do Hospital Veterinário da Universidade Metropolitana de Santos – São Paulo – Brasil. Durante o período do estudo, foram atendidas 317 fêmeas com diagnóstico histopatológico de neoplasia, dos quais, 170 se referiam a lesões mamárias epiteliais distribuídas em 13 tumores benignos, 152 malignos (89,4% dos diagnósticos) e 5 lesões epiteliais não-neoplásicas (hiperplasia ductal). O tumor mais frequente foi o carcinoma tubular (38,2% dos tumores malignos) e tumores de grau I, respondendo por 73,0% do total diagnosticado. Estudos de sobrevida apontaram para o estadiamento clínico das neoplasias mamárias caninas como importante fator prognóstico, e na análise multivariada, diâmetro do tumor, grau histológico, quimioterapia adjuvante e recorrência apresentaram-se como covariáveis com valor preditivo de sobrevida. Levando-se em conta a elevada prevalência de carcinoma tubular simples na população canina de Santos, pode-se considerá-la como promissor modelo translacional para o estudo da doença.<strong></strong> | Tumors of the mammary glands are the most common neoplasms in dogs in our country; however, there are few Brazilian reports dedicated to clinicopathological and survival studies about this disease. This report aims the clinical and pathological study of canine mammary tumors in the Santos Metropolitan Region, an area in Sao Paulo state with an estimated canine population of 120,000 animals. Data of 14,298 dogs were collected retrospectively from the medical records of the Veterinary Medical Teaching Hospital of the Metropolitan University of Santos – São Paulo – Brazil. During the study period, from records of 317 females with histopathological diagnosis of neoplasia, 170 were mammary epithelial lesions distributed in 13 benign tumors, 152 malignant (89.4% of diagnosis) and 5 non-neoplasic epithelial lesions (ductal hyperplasia). The highest prevalent malignant tumor was tubular carcinoma (38.2% of diagnosis) and Grade I tumors, corresponding to 73.0% of all diagnosis. The results have shown clinical staging of canine mammary neoplasms as an important prognostic survival factor and, in a multivariate analysis, tumor diameter, tumor grade, adjuvant chemotherapy and recurrence as covariates with predictive value for survival. Moreover, the high prevalence of tubular carcinoma qualifies the canine population ofSantosas a promising model for the translational study of this disease.
Показать больше [+] Меньше [-]Ultrasonography as an ancillary method for the positioning of markers in equine motion analysis Полный текст
2014
Luanna Ferreira Fasanelo Gomes | Stefano Carlo Filippo Hagen | Ricardo Machado Leite de Barros | Antônio Queiroz-Neto
Ultrasonography as an ancillary method for the positioning of markers in equine motion analysis Полный текст
2014
Luanna Ferreira Fasanelo Gomes | Stefano Carlo Filippo Hagen | Ricardo Machado Leite de Barros | Antônio Queiroz-Neto
Kinematic motion analysis is based on the reconstruction of selected bony anatomical landmarks identified by surface markers. Anatomical landmarks generally do not correspond to points but rather to relatively large and curved areas and their identification by palpation is not easy. Precise placement of surface markers is even more difficult and there is great variability between operators. In this study 16 examiners were asked to identify the lateral border of the left ischial tuberosity in a horse using palpation and ultrasonography for placement of a corresponding skin surface marker. Images of each marking procedure were captured using two video cameras and processed using the DVideow videogrammetry. A custom-written Matlab code was used to determine the position of the respective vectors. The positions of the markers were then compared to assess inter-examiner variability and the precision of the methods employed using the Bartletttest and the paired t-test respectively. Ultrasonography significantly improved the location of the anatomical landmark by each examiner (p = 0.04) and reduced the variability in the position of the surface marker when compared to palpation (p = 0.0028). The variability of the calculated distances (mean ± SD) was 2.89 ± 2.24 cm and 1.63 ± 0.98 cm using palpation and ultrasonography respectively. Ultrasound guidance reduced inter-examiner variability and allowed visualization of the corresponding bony anatomical landmark.
Показать больше [+] Меньше [-]Utilização da ultrassonografia como método auxiliar no posicionamento de marcadores em análise de movimento de equinos | Ultrasonography as an ancillary method for the positioning of markers in equine motion analysis Полный текст
2014
Luanna Ferreira Fasanelo Gomes | Stefano Carlo Filippo Hagen | Ricardo Machado Leite de Barros | Antônio Queiroz-Neto
Kinematic motion analysis is based on the reconstruction of selected bony anatomical landmarks identified by surface markers. Anatomical landmarks generally do not correspond to points but rather to relatively large and curved areas and their identification by palpation is not easy. Precise placement of surface markers is even more difficult and there is great variability between operators. In this study 16 examiners were asked to identify the lateral border of the left ischial tuberosity in a horse using palpation and ultrasonography for placement of a corresponding skin surface marker. Images of each marking procedure were captured using two video cameras and processed using the DVideow videogrammetry. A custom-written Matlab code was used to determine the position of the respective vectors. The positions of the markers were then compared to assess inter-examiner variability and the precision of the methods employed using the Bartletttest and the paired t-test respectively. Ultrasonography significantly improved the location of the anatomical landmark by each examiner (p = 0.04) and reduced the variability in the position of the surface marker when compared to palpation (p = 0.0028). The variability of the calculated distances (mean ± SD) was 2.89 ± 2.24 cm and 1.63 ± 0.98 cm using palpation and ultrasonography respectively. Ultrasound guidance reduced inter-examiner variability and allowed visualization of the corresponding bony anatomical landmark.<strong></strong> | A análise cinemática do movimento é baseada na reconstrução de pontos anatômicos específicos identificados por marcadores de superfície. Esses pontos de referência geralmente não correspondem a pontos, mas a áreas relativamente grandes e curvas de difícil identificação pela palpação. A colocação precisa dos marcadores de superfície é ainda mais difícil e há grande variabilidade entre examinadores. Neste estudo 16 examinadores foram submetidos à identificação da borda lateral da tuberosidade isquiática esquerda de um cavalo usando a palpação e a ultrassonografia para colocar um marcador de superfície. Imagens de cada procedimento de marcação foram adquiridas utilizando duas câmeras de vídeo digital e processadas utilizando o sistema de videogrametria DVideow. O software Matlab foi usado para determinar a posição dos respectivos vetores. As posições dos marcadores foram comparadas para avaliar a variabilidade entre os examinadores e a precisão do método empregado através do teste de Bartlett e teste t pareado respectivamente. A ultrassonografia melhorou significativamente a localização dos pontos anatômicos de cada examinador (p = 0,04) e reduziu a variabilidade no posicionamento dos marcadores de superfície quando comparada a palpação (p = 0,0028). A variabilidade das distâncias calculadas (média ± dp) foi de 2,89 ± 2,24 cm e 1,63 ± 0,98 cm usando a palpação e a ultrassonografia respectivamente. A ultrassonografia reduziu a variabilidade entre examinadores e permitiu a visualização do ponto anatômico correspondente.
Показать больше [+] Меньше [-]Feasibility of grazing sheep production systems using long-term economic indicators and the methodology of the soil expectation value Полный текст
2014
Thayla Sara Soares Stivari | Rafael Felice Fan Chen | Augusto Hauber Gameiro | Alda Lúcia Gomes Monteiro | Camila Raineri | Juliana Batista Andrade Silva
Feasibility of grazing sheep production systems using long-term economic indicators and the methodology of the soil expectation value Полный текст
2014
Thayla Sara Soares Stivari | Rafael Felice Fan Chen | Augusto Hauber Gameiro | Alda Lúcia Gomes Monteiro | Camila Raineri | Juliana Batista Andrade Silva
Due to the high value of land in Brazil, it is necessary to conduct studies about the economic feasibility of the choice of production strategy. The soil expectation value determines the maximum purchase price of the bare land, considering the horizon of infinite time. Therefore, this methodology was used to assess the financial-economic feasibility of sheep production systems in pasture with either creep feeding or creep grazing termination of unweaned lambs. Six scenarios were proposed to evaluate the economic feasibility, according to the finishing strategy, the dry matter (DM) offer per kg of body weight (BW) per day (12% or 8%) and the corresponding percentage of white clover supplementary pasture area (30% to 50% - relative to the main pasture area). The soil expectation value was positive in all of the evaluated systems Creep grazing finishing system with 8% DM offer per kg BW and with 30% of reduction on the white glover area resulted in the largest financial return (R$ 289,043.71) and the highest internal return rate (1.74%). The most economically attractive scenario, however, was the creep feeding finishing system with 8% DM offer per kg of BW, which allowed expenses with the land as high as R$ 22,950.68 per hectare. Adjustments in the main pasture offer, which do not compromise animal performance, can enable economic improvement on the feasibility of grazing sheep production systems. The soil expectation value can be used as an economic indicator in the choice of location or expansion of grazing sheep farms.
Показать больше [+] Меньше [-]Viabilidade de sistemas de produção de ovinos a pasto utilizando indicadores econômicos de longo prazo e a metodologia do valor esperado da terra | Feasibility of grazing sheep production systems using long-term economic indicators and the methodology of the soil expectation value Полный текст
2014
Thayla Sara Soares Stivari | Rafael Felice Fan Chen | Augusto Hauber Gameiro | Alda Lúcia Gomes Monteiro | Camila Raineri | Juliana Batista Andrade Silva
Due to the high value of land in Brazil, it is necessary to conduct studies about the economic feasibility of the choice of production strategy. The soil expectation value determines the maximum purchase price of the bare land, considering the horizon of infinite time. Therefore, this methodology was used to assess the financial-economic feasibility of sheep production systems in pasture with either creep feeding or creep grazing termination of unweaned lambs. Six scenarios were proposed to evaluate the economic feasibility, according to the finishing strategy, the dry matter (DM) offer per kg of body weight (BW) per day (12% or 8%) and the corresponding percentage of white clover supplementary pasture area (30% to 50% - relative to the main pasture area). The soil expectation value was positive in all of the evaluated systems Creep grazing finishing system with 8% DM offer per kg BW and with 30% of reduction on the white glover area resulted in the largest financial return (R$ 289,043.71) and the highest internal return rate (1.74%). The most economically attractive scenario, however, was the creep feeding finishing system with 8% DM offer per kg of BW, which allowed expenses with the land as high as R$ 22,950.68 per hectare. Adjustments in the main pasture offer, which do not compromise animal performance, can enable economic improvement on the feasibility of grazing sheep production systems. The soil expectation value can be used as an economic indicator in the choice of location or expansion of grazing sheep farms. | Devido ao valor elevado da terra no Brasil, é necessária a realização de estudos de viabilidade econômica durante a escolha da estratégia de produção. O valor esperado da terra determina o preço máximo de compra da terra nua, considerando o horizonte de tempo infinito. Portanto, essa metodologia foi utilizada para avaliar a viabilidade econômico-financeira de sistemas de produção de ovinos em pastagem com terminação em creep feeding ou creep grazing de cordeiros não desmamados. Seis cenários foram propostos para avaliar a viabilidade econômica de acordo com a estratégia de terminação, a oferta de matéria seca (MS) por kg de peso vivo (PV) por dia (12% ou 8%) e a área de pastagem suplementar correspondente de trevo branco (30% a 50% – em relação à área de pastagem principal). O valor esperado da terra foi positivo em todos os sistemas avaliados. O sistema com terminação em creep grazing com 8% de oferta de MS/PV e com redução da área suplementar para 30% apresentou o maior retorno financeiro (R$ 289.043,71) e a maior taxa interna de retorno (1,74%). No entanto, o cenário economicamente mais atraente foi o sistema de creep feeding com 8% de oferta de MS/PV, permitindo despesas de até R$ 22.950,68 por hectare. Ajustes na oferta da pastagem principal, que não comprometam o desempenho do animal, podem permitir melhores resultados econômicos de sistemas de produção de ovinos em pastagem. O valor esperado da terra pode ser usado como um indicador econômico na escolha da localização ou ampliação de sistemas de produção de ovinos em pastejo.
Показать больше [+] Меньше [-]Central and peripheral oxytocin profiles during milking in ewes Полный текст
2014
João Carlos Bochini | Renato Duarte Alvisi | João Alberto Negrão | Erica Engelberg Teixeira da Silva Hucke | Luciano Freitas Felicio
Central and peripheral oxytocin profiles during milking in ewes Полный текст
2014
João Carlos Bochini | Renato Duarte Alvisi | João Alberto Negrão | Erica Engelberg Teixeira da Silva Hucke | Luciano Freitas Felicio
The present work investigated the possible relationship between central and peripheral oxytocin (OT) release during milking in experimental ewes. Ten multiparous ewes were divided into four groups according to milk ejection stimuli: exclusive machine milking (EM), mixed-management milking and suckling, lambs separated during the night and reunited with their mother after morning milking (MMS); mixed-management with manual milking (MMM), and exclusive suckling (ES) lambs also separated during the night. Simultaneous sampling of cerebrospinal fluid (CSF) and blood was performed during milking. The means, standard deviations, variation coefficients, and minimum and maximum CSF and plasma OT concentrations were the following, respectively: 257.88 ± 265.90 pg/ml, 103.11%, and 11.70 and 1000.00 pg/ml. No statistically significant correlations were found between OT concentrations in the CSF and plasma samples (EM: -0.26; ES: -0.19; MMM: 0.05; MMS: 0.04). The OT concentration in CSF was not influenced by milk ejection stimuli, although plasma OT was higher in the MMM (679.80 ± 25.63) and MMS (591.82 ± 30.56) groups compared with the EM and ES groups. Additionally, plasma OT concentrations were higher in the OME group (381.04 ± 22.09) compared with the AE group (218.82 ± 27.04). In conclusion, no positive correlations were found between central and peripheral OT concentrations during milking and suckling. Plasma OT concentrations differed as a function of milking management and had consequences for both milk ejection and production. Plasma but not CSF oxytocin concentrations were influenced by different milk ejection stimuli.
Показать больше [+] Меньше [-]Perfil de ocitocinea central e periférica em ovelhas lactantes | Central and peripheral oxytocin profiles during milking in ewes Полный текст
2014
João Carlos Bochini | Renato Duarte Alvisi | João Alberto Negrão | Erica Engelberg Teixeira da Silva Hucke | Luciano Freitas Felicio
The present work investigated the possible relationship between central and peripheral oxytocin (OT) release during milking in experimental ewes. Ten multiparous ewes were divided into four groups according to milk ejection stimuli: exclusive machine milking (EM), mixed-management milking and suckling, lambs separated during the night and reunited with their mother after morning milking (MMS); mixed-management with manual milking (MMM), and exclusive suckling (ES) lambs also separated during the night. Simultaneous sampling of cerebrospinal fluid (CSF) and blood was performed during milking. The means, standard deviations, variation coefficients, and minimum and maximum CSF and plasma OT concentrations were the following, respectively: 257.88 ± 265.90 pg/ml, 103.11%, and 11.70 and 1000.00 pg/ml. No statistically significant correlations were found between OT concentrations in the CSF and plasma samples (EM: -0.26; ES: -0.19; MMM: 0.05; MMS: 0.04). The OT concentration in CSF was not influenced by milk ejection stimuli, although plasma OT was higher in the MMM (679.80 ± 25.63) and MMS (591.82 ± 30.56) groups compared with the EM and ES groups. Additionally, plasma OT concentrations were higher in the OME group (381.04 ± 22.09) compared with the AE group (218.82 ± 27.04). In conclusion, no positive correlations were found between central and peripheral OT concentrations during milking and suckling. Plasma OT concentrations differed as a function of milking management and had consequences for both milk ejection and production. Plasma but not CSF oxytocin concentrations were influenced by different milk ejection stimuli. | Foi investigada a possível relação entre as concentrações de ocitocina no líquido céfalo-raquidiano e no soro em diferentes formas de ordenha em ovinos. Foram utilizadas dez ovelhas multíparas divididas em quatro grupos de acordo com o estímulo para ejeção do leite: ordenha exclusivamente mecânica (EM), ordenha mista mecânica e mamada com os carneiros separados das mães durante a noite e reunidos a elas pela manhã para amamentação (MMS); ordenha mista com ordenha manual (MMS); apenas amamentação natural (ES). Foram coletadas amostras de fluido cerebroespinhal e de sangue simultaneamente durante as ordenhas. A média, coeficiente de variação e valores máximos e mínimos de ocitocina do plasma foram respectivamente 257,88 ± 265,90 pg/ml, 103,11%, e 11,70 e 1000,00 pg/ml. Não foram encontradas correlações entre as concentrações centrais e plasmáticas de ocitocina (EM: -0,26; ES: -0,19; MMM: 0,05; MMS: 0,04). Não foi evidenciada influência do tipo de estímulo para ejeção do leite nas concentrações centrais de ocitocina. Entretanto, as concentrações plasmáticas de ocitocina foram maiores nos grupos MMM (679,80 ± 25,63) e MMS (591,82 ± 30,56) quando comparadas as dos grupos EM e ES. Alem disso, as concentrações plasmáticas de ocitocina foram maiores no grupo de OME (381,04 ± 22,09) em relação ao grupo AE (218,82 ± 27,04). Os resultados obtidos sugerem que as concentrações plasmáticas de ocitocina são mais sensíveis ao tipo de ordenha que as concentrações centrais desse hormônio.
Показать больше [+] Меньше [-]Histologia do intestino do avestruz (Struthio camelus, Linnaeus 1758) | Histology of ostrich intestine (Struthio camelus, Linnaeus 1758) Полный текст
2014
Gisele Saviani | Roselaine Ponso | Bruno Cogliati | Cintia Maria Monteiro de Araújo | José Manoel dos Santos | Arani Nanci Bonfim Mariano | Ricardo de Albuquerque
Regardless of the ostrich (Struthio camelus) share many adaptations to other evolutionary present birds, these animals show some peculiar anatomical features such as their digestive tract than the colon is greater than the cecum. For some time this bird has been economically exploited and especially as an alternative source of animal protein for human consumption. This study examined the histological bowel ostrich produced in good environmental management and nutrition. Thirteen ostriches were used, with 18 to 30 months old, from Brazil Ostrich company, and sent for slaughter in Slaughterhouse School, University of São Paulo Campus Administrative Pirassununga. The animals were killed with pneumatic gun and after bleeding and evisceration were collected, samples of different intestinal segments: duodenum, jejunum, ileum and cecum double. The materials were processed, stained with hematoxylin - eosin (HE) and examined under brightfield microscopy. The results showed that the villi are present in the duodenum but not exist in the cecum. Of the four intestinal segments examined the cecum showed the highest number of goblet cells. Lymph nodes and lymphocytes were observed in all segments examined. In the cecum lymph nodes are added to form the Peyer’s patch. The plan of histological intestinal segments examined followed the pattern observed in other domestic mammals and birds. The knowledge of the histology of the intestines of these animals can provide insight for comparative assessment procedures for environmental management and nutrition that may increase the levels of production and productivity of this livestock activity. | A despeito de o avestruz (S<em>truthio camelus</em>) compartilhar muitas adaptações evolucionárias presentes em outras aves, estes animais apresentam algumas características anatômicas peculiares, como é o caso do seu tubo digestivo em que o cólon é maior que o ceco. Há algum tempo, essa ave tem sido explorada econômicamente e principalmente como fonte alternativa de proteína animal na alimentação humana. O presente trabalho analisou os aspectos histológicos do intestino de avestruzes produzidos em boas condições de manejo ambiental e nutricional. Foram utilizados 13 avestruzes, com 18 a 30 meses de idade, provenientes da empresa Brasil Ostrich, e encaminhados para o abate no Abatedouro Escola da Universidade de São Paulo, Campus Administrativo de Pirassununga. Os animais foram abatidos com pistola pneumática e, após a sangria e evisceração, foram colhidas amostras de diferentes segmentos do intestino: duodeno, jejuno, íleo e ceco duplo. Os materiais foram processados, corados pela técnica de hematoxilina-eosina (H-E) e examinados em microscopia de campo claro. Os resultados obtidos revelaram que as vilosidades estão presentes no duodeno, porém, não existem no ceco. Dos quatro segmentos intestinais examinados, o ceco foi o que apresentou maior número de células caliciformes. Os nódulos linfáticos e os linfócitos foram observados em todos os segmentos examinados. No ceco, os nódulos linfáticos se agregam para constituir a placa de Peyer. O plano histológico dos segmentos intestinais examinados seguiu o padrão observado nos mamíferos domésticos e em outras aves. O conhecimento da histologia dos intestinos desses animais pode oferecer subsídios para a avaliação comparativa de procedimentos de manejo ambiental e nutricional que possam aumentar os níveis de produção e produtividade dessa atividade pecuária.
Показать больше [+] Меньше [-]Development of bovine abomasum in prenatal period | Desenvolvimento do abomaso de bovinos no período pré-natal Полный текст
2014
Gabriela Mansano do Nascimento | Silvia Helena Venturoli Perri | Roberto Gameiro de Carvalho | Cristina Maria Rodrigues Monteiro
<!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG/> </o:OfficeDocumentSettings></xml><![endif]--><p class="MsoNormal"><span style="mso-ansi-language: EN-US;" lang="EN-US">For this purpose, samples of Nelore fetus abomasum (Bos taurus indicus) were classified into five groups: 1 – fetuses with 9 to 15 weeks (8 to 21 cm) of gestation, 2 – fetuses with 16 to 22 weeks (23 to 37 cm), 3 – fetuses at 23 to 29 weeks (40 to 58 cm) 4 – fetuses with 30 to 36 weeks (61 to 77 cm) and 5 – fetuses with 37 to 43 weeks (79 to 88 cm). Histologic sections were stained with Hematoxylin and eosin, picrosirius and Mallory’s trichrome methods and examined under light microscopy. In fetus with 11 cm of length showed deep mucosa, wide folds and villi lined by single cylindrical epithelium, lamina propria, submucosa, muscular sublayers and serosa. Fetus with 13.5 cm, villi were evident and muscular layer of the mucosa was formed. At 16.5 cm, could be seen an increase in mucosa glands size and number. In fetus of 26 cm, showed gastric mucous glands at great number and ramifications. At 29 cm large folds, were observed. At 37 cm, showed glandular epithelium, muscular layer of the mucosa and muscular layer well development. Fetus with 42 cm, showed deep glands with corresponding mucous cells surrounding by thin lamina propria. It was concluded that the histomorphometric values of muscular layer and total abomasum wall were increased for all groups except for groups 4 and 5 which there was a decline of values, without presenting a significant difference and there was no continuous pattern of growth for other components of abomasum wall.</span><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves/> <w:TrackFormatting/> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF/> <w:LidThemeOther>PT-BR</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> 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normal";mso-tstyle-rowband-size:0;mso-tstyle-colband-size:0;mso-style-noshow:yes;mso-style-priority:99;mso-style-parent:"";mso-padding-alt:0cm 5.4pt 0cm 5.4pt;mso-para-margin-top:0cm;mso-para-margin-right:0cm;mso-para-margin-bottom:10.0pt;mso-para-margin-left:0cm;line-height:115%;mso-pagination:widow-orphan;font-size:11.0pt;font-family:"Calibri","sans-serif";mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-fareast-language:EN-US;}</style><![endif]--> | <!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG/> </o:OfficeDocumentSettings></xml><![endif]--><p class="MsoNormal"><span style="font-size: 10.0pt; line-height: 115%; font-family: 'MinionPro-Regular','serif'; mso-bidi-font-family: MinionPro-Regular;">Para esta pesquisa, amostras de abomaso de fetos da raça Nelore </span><em><span style="font-size: 10.0pt; line-height: 115%; font-family: 'MinionPro-It','serif'; mso-bidi-font-family: MinionPro-It;">(Bos taurus indicus) </span></em><span style="font-size: 10.0pt; line-height: 115%; font-family: 'MinionPro-Regular','serif'; mso-bidi-font-family: MinionPro-Regular;">foram divididos em cinco grupos: 1º – fetos com 9 a 15 semanas (8 a 21cm) de gestação, 2º – fetos com 16 a 22 semanas (23 a 37 cm), 3º – fetos com 23 a 29 semanas (40 a 58 cm), 4º – fetos com 30 a 36 semanas (61 a 77 cm) e 5º – fetos com 37 a 43 semanas (79 a 88 cm). Os cortes histológicos foram corados com Hematoxilina e Eosina, picrossirius e Tricrômico de Mallory e examinados ao microscópio de luz. No feto de 11 cm, observaram-se pregas mucosas largas, vilosidades revestidas por epitélio simples cilíndrico, lâmina própria, tela submucosa, túnica muscular com estratos interno e externo e serosa. Aos 13,5 cm, as vilosidades eram evidentes com formação da lâmina muscular da mucosa. Aos 16,5 cm, houve aumento em número e tamanho das glândulas mucosas. No feto de 26 cm, observou-se aumento das túnicas e ramificações das glândulas. Aos 29 cm, observou-se aumento no tamanho das pregas. Com 37 cm, houve aumento do epitélio glandular, lâmina muscular da mucosa e túnica muscular. Com 42 cm, as glândulas eram profundas com células mucosas e lâmina própria delgada. Concluiu-se que as os valores histomorfométricos da túnica muscular e parede total do abomaso foram crescentes para todos os grupos, com exceção dos grupos 4 e 5 para os quais houve queda dos valores, sem diferença significante e não houve crescimento contínuo para os demais constituintes da parede do abomaso.</span>
Показать больше [+] Меньше [-]Biometric development and performance of broilers submitted to different periods of post-hatching fasting | Desenvolvimento biométrico e desempenho de frangos de corte submetidos a diferentes períodos de jejum pós-eclosão Полный текст
2014
Letícia Souza Silva Carvalho | Camila Alves Machado | Naiara Simarro Fagundes | Fernanda Heloisa Litz | Evandro de Abreu Fernandes
Foram avaliados os efeitos do tempo de jejum pós-eclosão sobre a biometria dos órgãos do trato digestório e o desempenho zootécnico de frangos de corte. Foram utilizadas 640 aves, sexadas, distribuídas em delineamento experimental inteiramente casualizado com quatro períodos de jejum pós-eclosão (0, 12, 24 e 36 horas) e quatro repetições de 40 aves cada (20 fêmeas e 20 machos). A avaliação do peso vivo e da biometria dos órgãos no alojamento das aves revelou perda de peso relativo do saco vitelino e que a redução no peso vivo nesta fase foi tanto maior quanto maior o tempo decorrido entre nascimento e alojamento. O jejum influenciou o peso relativo de todos os órgãos do trato digestório, exceto para esôfago + papo que permaneceram inalterados. Com o aumento do tempo de jejum houve aumento do peso relativo de proventrículo + moela, intestino delgado + pâncreas, intestino grosso e fígado + vesícula biliar. O peso vivo aos 7 e 21 dias de idade foi maior nas aves que permaneceram em restrição de 24 e 36 horas de jejum. Todavia, as demais variáveis de desempenho não foram influenciadas pelo jejum. Portanto, o uso de até 36 horas de jejum póseclosão afetou o desenvolvimento biométrico do trato digestório, no entanto, o desempenho zootécnico dos frangos de corte aos 42 dias de idade não foi influenciado pela restrição alimentar. | The objective of this study was to evaluate the effects of fasting time on biometrics organ digestive tract and performance of broilers. A total of 640 sexed birds were, distributed according to a completely randomized design with four periods of fasting after hatching (0, 12, 24 and 36 hours) and four replicates of 40 birds each (20 females and 20 males). The evaluation of body weight and biometrics of the organs during the housing of the birds showed that there was loss of relative weight of the yolk sac and reduction in weight as they increased the time between birth and accommodation. Fasting influenced the relative weight of all of the digestive tract, except for esophagus + craw that remained unchanged. With the increasing fasting time there was an increase in relative weight of the proventriculus + gizzard, small intestine + pancreas, large intestine, liver + gallbladder. The live weight at 7 and 21 days of age was higher in birds that remained in restriction of 24 and 36 hours of fasting. However, the other performance variables were not affected by fasting compared with birds with no fasting. Therefore, the use of up to 36 hours post-hatching fasting affects the development biometric digestive tract, however the production performance of broiler at 42 days of age is not affected by food restriction.
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