A preliminary study of nearinfraredreflectance spectroscopy (NIRS)for the feasibility of analytical monitoring ofamino acids and total protein compositionsin edible bird’s nest (EBN) was conducted.The training (n=134) and validation sets todevelop the equations were built with localunprocessed EBN samples sourced fromdifferent states of Malaysia. The regressionmethod employed was modified partialleast-squares (MPLS). The values ofstandard error for cross validation (SECV)and the coefficient of determination (r2)of the calibrations of these constituentsfor use to predict of amino acids in EBNwere determined, but with a low predictiveability. To find an acceptable accuracyfor each constituent is to increase thenumber of training samples. The findings,however, showed a potential alternativefor the implementation of near-infraredreflectance spectroscopy technology inthis field of analysis.

Colibacillosis is animportant disease affecting the poultryindustry in many countries, caused bythe Avian Pathogenic E. coli (APEC):it manifests as various clinical signs. Itcontributes significantly to economicloss for poultry farmers as a result ofhigh mortality and morbidity in poultry.To overcome this, antibiotics have beenwidely used to eliminate E. coli infectionin poultry farms in recent years. Treatmentwith antibiotics has been considered as avital regimen to control E. coli infectionat the farm level for many years. However,high frequency of antibiotic resistance ofE. coli isolates from chicken has becomethe centre of attention due to public healthimportance. The aim of the present study isto determine the multidrug resistant profilesof E. coli strains isolated from chicken.E. coli isolates obtained from clinicalcases were re-identified and classified byconventional methods. Multidrug resistantprofiles against 13 different antibiotics of125 E. coli isolates were determined byusing disk diffusion method accordingto Clinical Laboratory Standard Institute(CLSI). Antibiogram revealed that 81.6%of the E. coli isolates showed multidrugresistant profiles to different antibiotics.Most of the E.coli isolates were highlyresistant to erythromycin (52.8%), followedwith tetracycline (52.0%), spectinomycin(39.2%), trimethoprim (38.4%) andflumequin (37.6%). Out of 125 isolatestested, 19.2% were resistant to more thaneight antibiotics, with one isolates found tobe multidrug resistant to most of antibioticsexcept polymyxin B. These findings alsodemonstrated that most of the isolateswere susceptible to antibiotics commonlyused for E.coli infections treatment inpoultry with lowest resistant score againstpolymyxin B (92.8%) and colistin (92.0%).Moderate resistant profiles were observedtowards amoxycilin (25.6%), apramycin(16%), kanamycin (8.8%) and streptomycin(8.0%). High percentage of multidrugresistance was found among the E. coliisolated from chicken as an indicator tomore serious problems in animal health.Therefore, continuous surveillance of antibiotic resistance profiles in chicken andother food animals is crucial to ensure foodchain safety

Stress is commonly used to describe the detrimental effectsof a variety of conditions surrounding animals on their health performance. Environmental stress causes an increasein oxidative stress and an imbalance in antioxidant status. Oxidative damage increases in stressed poultry when the plasma antioxidant vitamins and minerals such as vitamin C, E, folic acid, and zinclevels declined. Stress factors in birds involve many elements of the transport processes which may be detrimental to the birds. These factors include alteration in atmospheric temperature, poor handlingby man, removal of feed and water (starvation), high ambient temperature (AT) and relative humidity (RH). Other factors incude vaccination, disease conditions such as coccidiosis, novelty,confinement, motion, noise, microthermal core within the vehicle and the use of inappropriate vehicles. Live birds of all ages are being transported mainly by roads to their various destinations and across different ecological zones throughout the year. Mortality increaseswith journey length. The adverse effects of these factors and their combinations may range from mild discomfort and aversion to death. This review was aimed at providing comprehensive information on the role of vitamin C in combating stress factors in poultry with the view of exploring its potentials for future research undertaking.Information compiled in this review were obtained from search engines such as Elsevier, Pubmed, Springer, Medline, Science Direct, Google Scholar, and a library search for articles published in peerreviewedjournals. 100 to 200 mg/kg feed of vitamin C is capable of converting stress factors in poultry and thereby improving the productivity.

The objectives of the present study were to determine the infection rate of equine piroplasmosis (EP) in horses and ponies in Kelantan,Malaysia and compare the microscopic examination with competitive enzymelinked immunosorbent assay (cELISA) test as methods for diagnosis of EP. 306 blood samples were randomly collected from equids including 148 horses and 158 ponies in various districts of Kelantan, from September 2013 to March 2014. Based on microscopic examination of the staining blood smears, the infection rates ofTheileria equi, Babesia caballi and of both infections in horses were 19.59%, 25% and 8.78% respectively, whereas in ponies theinfection rates were 14.55%, 19.62%, and 5.69% respectively. Based on cELISA test, the infection rates of T. equi, B. caballi and of both infections in horses were 50.67%, 62.16% and 33.10% respectively,whereas in ponies, the infection rates were 51.89%, 63.92% and 35.44% respectively. No significant difference were observed between equids species associated with a seroprevalence of T. equi, B. caballi andof both infections (P≤ 0.05). According to the Kappa value there was no compatibility between microscopic examination and cELISA on the diagnosis of T. equi, B. caballi and of both infections which were 0.235, 0.013 and 0.080 respectively. In conclusion, the current results for this research work indicate that equine piroplasmosis is widespread in Kelantan, Malaysia and cELISA test is more efficientthan microscopic examination for diagnosis of EP.

Different types of skin lesions and their distribution in dogs withrecurrent pyoderma along with haematobiochemicalfindings were recorded in this study. Dogs with recurrent superficial pyoderma revealed papules, pustules, crusted papules, erythema, alopecia,crusts, scales, plaques, hyper-pigmentation and pruritus. Dogs affected with recurrent deep pyoderma had symptoms like papules,pustules, cellulitis, ulcers, crusted papules, nodules, fistulous tracts, alopecia, scale formation, crusts, hyper-pigmentation,erosions and furunculosis, pain and edema. The major locations of lesions for recurrent superficial pyoderma included lateral abdomen, lateral thorax and dorsum, axilla, groin, hind limb, foot, neck and fore limb and head. Lesions of recurrent deep pyoderma were predominantly observed over dorsum and lateral abdomen followedby head, neck, hind limb, lower abdomen, axilla and groin, forelimb and lateral thorax. Haemato-biochemical findings revealed leucocytosis, increased in absolute neutrophil count, eosinophil count and high serum cholesterol levels. Affected dogs also had decreased haemoglobin concentration, total erythrocyte count and serum albumin levels.

In Malaysia there are public opinion that goat meat contributes to high blood pressure and high blood cholesterol level that can lead to cardiovascular diseases. The aim of this study was to investigate this perception. 10 subjects were involved in the study, five men and five women. They were given fixed amount of goat meat soup with two slices of bread and a cup of sugarless barley drink. Pairedsamplest-test was used to compare the differences in the subjects’ blood pressure and cholesterol level between pre- and post- intake of goat meat. The results of this study shows that consumption of goatmeat does not cause increase risk of blood pressure and cholesterol level.

Toxoplasma gondii is a protozoan parasite that causes toxoplasmosis in humans and animals. It belongs to the phylum Apicomplexa, subclass Coccidiasina and family Sarcocystidae. The Felidae family is thedefinitive host for this disease where the parasites undergo a sexual cycle of replication (oocysts). In this study, cat faeces were collected from private clinics around Johore Bahru, Peninsular Malaysia. A total of 61 samples were tested using microscopy to detect for presence of T. gondii oocyst via two methods; namelythe Modified Kato-Katz with Kinyoun staining and Sheather’s sugar floatation methods. The results showed that 40.98% of the faecal samples tested were positive for T. gondii oocysts. These two methodswere successfully used in diagnosing toxoplasmosis in cats in this study. Morphological approaches for Toxoplasma oocysts identification have been neglected in recent years, due to the upsurge of moreprecise technologies. This study suggests that this modified technique could be introduced for screening and detection of oocysts excreted in faeces of suspected animals of the Felidae family.

This study was conducted to investigate the high mortality of young lambs in two sheep farms in Pekan, Pahang over a period of 3 years. Samples from postmortem of 1,451 lambs below one year of age by a farm veterinarian were submitted for laboratory diagnosis at the Bacteriology Section of the Regional Veterinary Diagnostic Laboratory in Kuantan. Escherichia coli is the most commonly recorded bacteria with 161 lambs diagnosed in 2013. In 2014 and 2015, there was a decrease in occurrence of E. coli related deaths, with 120 and 75lambs respectively. A total of 25% of the cases showed Escherichia coli positive by culture on blood agar and MacConkey agar, and confirmed by biochemical tests. A total of 21% of the cases were positive for staphylococcus sp, 3% and 6% for Streptococcus sp and Klebsiella pneumonia, respectively. Other bacteria were isolated in 45% of the cases. It was further noted that a total of 285 lambs between the ages of one to four months of age followed by 58 lambs (20%) less than one month old had E.coli isolation. It is also noteworthy that there were 10 lambs with E.coli infection in one to fourteen day-old lambs during the 3-year period from January 2013 to December 2015. This information was collated as a result of routine diagnosis of field cases submitted and with the intention of highlighting the common pathogens causing high mortality in local small ruminant farms so that preventive action may be taken for future farming ventures. E. coli infections or Colibacillosis is an important finding and indicator of poor management including poor nutrition, hygiene and environmental contamination which can reduce animal immunity and render it susceptible to other infections.

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA) a contagious and infectious disease in smallruminants. This report describes a case of CLA in a Boer cross goat infected with Corynebacterium pseudotuberculosis. The manifestation of abscess in the superficial lymph node presented as a mass ofapproximately 5 cm by 5 cm in diameter at the base of the ear. The mass was solid and non-movable upon palpation. The mass was incised and the caseous material was aspirated and sent to the bacteriologylaboratory where Corynebacterium pseudotuberculosis was isolated. Thewound was cleaned and flushed with diluted chlorhexidine and iodine solutions daily. This procedure aids in the complete healing of the wound after a few weeks. However, the agent of the disease persistsin the animal in its entire life and culling is recommended to prevent the spread of the disease in the herd.

OBJECTIVE To evaluate the pharmacokinetics of zonisamide following rectal administration of 20 or 30 mg/kg suspended in sterile water or polyethylene glycol (PEG) to healthy dogs and determine whether either dose resulted in plasma zonisamide concentrations within the recommended therapeutic target range (10 to 40 μg/mL). ANIMALS 8 healthy mixed-breed dogs. PROCEDURES Each dog received each of 2 doses (20 or 30 mg/kg) of zonisamide suspended in each of 2 delivery substrates (sterile water or PEG) in a randomized crossover study with a 7-day washout period between phases. A blood sample was collected from each dog immediately before and at predetermined times for 48 hours after zonisamide administration. Plasma zonisamide concentrations were determined by high-performance liquid chromatography, and data were analyzed with a noncompartmental model. RESULTS Mean maximum plasma concentration, time to maximum plasma concentration, mean residence time, and elimination half-life did not differ significantly among the 4 treatments. The mean maximum plasma concentration for all 4 treatments was less than the therapeutic target range. The mean ± SD area under the concentration-time curve for the 30 mg/kg-in-water treatment (391.94 ± 237.00 h•μg/mL) was significantly greater than that for the 20 mg/kg-in-water (146.19 ± 66.27 h•μg/mL) and 20 mg/kg-in-PEG (87.09 ± 96.87 h•μg/mL) treatments. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that rectal administration of zonisamide at doses of 20 and 30 mg/kg failed to achieve plasma zonisamide concentrations within the recommended therapeutic target range. Therefore, rectal administration of zonisamide cannot be recommended as a suitable alternative to oral administration.