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Clinical and serological evaluations of induced Borrelia burgdorferi infection in dogs.
1988
Greene R.T. | Levine J.F. | Breitschwerdt E.B. | Walker R.L. | Berkhoff H.A. | Cullen J. | Nicholson W.L.
Adult Beagles were used to evaluate clinical signs and serologic response after inoculation with, or exposure to, Borrelia burgdorferi. An indirect immunofluorescent assay (IFA) and 2 ELISA were used to monitor the serologic response to B burgdorferi. Feeding infected ticks on 4 dogs (group 1) failed to cause seroconversion, and SC inoculation with 500 organisms caused minimal seroconversion in 2 of 4 dogs (group 2). At 56 days, approximately 3.01 X 10(8) B burgdorferi organisms were injected IV into group-1 dogs, and intraperitoneally into group-2 dogs. A control group of 4 dogs (group 3) had noninfected ticks feed on them, and then were given IV injection of physiologic saline solution. Increases in immunoglobulin M (IgM) titers were detected in 2 of 4 group-2 dogs approximately 7 days after the initial exposure. These titers returned to negligible values 20 days later. Immunoglobulin G titers increased approximately 10 days after the initial exposure and were mildly increased 56 days later, when dogs were exposed a second time. Both the IV and intraperitoneal injections (second exposures) resulted in increased IgM titers, which in both groups eventually returned to preexposure values after approximately 2 months. Immunoglobulin G titers increased within a week after the second exposure, and in 3 dogs monitored for 8 months, returned to negligible values after the 8-month period. One control dog had a slightly increased IgG titer 24 days after the second inoculation. The possibility of urine transmission is suggested. Clinical status, hemograms, serum biochemical profiles, ECG and results of urinalyses remained normal throughout the study. Borrelia burgdorferi was not isolated from either the blood or urine of these dogs. Gross or microscopic pathologic changes were not detected on necropsy.
Показать больше [+] Меньше [-]Eructation of gas through the gastroesophageal sphincter before and after gastric fundectomy in dogs.
1988
Strombeck D.R. | Turner W.D. | Harrold D.
Stimulated esophageal groove closure in adult goats.
1988
Mikhail M. | Brugere H. | Le Bars H. | Colvin H.W. Jr.
In healthy adult goats, closure of the esophageal groove was induced by thirst, IV administered vasopressin, and intracarotid administration of hypertonic NaCl solutions. The efficiency of stimulation was tested directly by visual inspection of the course taken by orally administered solutions through a ruminal or abomasal fistula, palpation of the lips of the esophageal groove through a ruminal fistula, and indirectly by following the glucose dynamics in the blood after oral administration of glucose solution. Esophageal groove closure was observed during drinking after a 48-hour period of water deprivation. Intracarotid administration of 1.5 ml of a saturated solution or 10.5 ml of a 1.5% solution of NaCl also stimulated groove closure; however, groove closure stimulated by administration of vasopressin is the most satisfactory procedure for passing compounds of therapeutic importance directly from the cardiac orifice to the abomasum.
Показать больше [+] Меньше [-]Estrone sulfate concentrations as an indicator of fetal demise in horses.
1988
Kasman L.H. | Hughes J.P. | Stabenfeldt G.H. | Starr M.D. | Lasley B.L.
Diprosopus in a Holstein calf.
1987
Hishinuma M. | Kohnose M. | Takahashi Y. | Kanagawa H.
Absolute and relative concentrations of immunoglobulins G, M, and A, and albumin in the lacteal secretion of sows of different lactation numbers.
1987
Klobasa F. | Butler J.E.
In vitro characterization of porcine juvenile articular cartilage.
1987
Lamar C.H. | Eller L.L. | Turek J.J.
Arterial hypotension and the development of postanesthetic myopathy in halothane-anesthetized horses.
1987
Grandy J.L. | Steffey E.P. | Hodgson D.S. | Woliner M.J.
Efficacy of Corynebacterium pseudotuberculosis bacterin for the immunologic protection of sheep against development of caseous lymphadenitis.
1987
LeaMaster B.R. | Shen D.T. | Gorham J.R. | Leathers C.W. | Wells H.D.
Frequency of association of noncytopathic bovine viral diarrhea virus with mononuclear leukocytes from persistently infected cattle.
1987
Bolin S.R. | Sacks J.M. | Crowder S.V.
