Blood is a good growth medium for bacteria by preventing the accumulation of toxic oxygen (peroxide and superoxide) and increasing the growth ability of these bacteria. The purpose of this study was to evaluate the ability of lysed sheep blood and lysed cow blood as a substitute for lysed horse blood on Campylobacter growth media, as well as evaluate the growth of Campylobacter from chicken meat samples on culture media supplemented with lysed sheep blood and lysed cow blood compared to the media cultures using lysed horse blood supplements. The concentration of lysed sheep blood, lysed cow blood, and lysed horse blood used was 5% each. The chicken meat samples used in this study were 30 samples. Based on the results, 14 samples of C. jejuni bacteria showed the ability to grow up to 107 and 16 samples up to 106. In the media added with lysed cow blood supplement only one sample was able to grow up to 106, 17 samples grew up to 105, and 12 samples grew up to 104. These results statistically used the one way analysis of variance (ANOVA) test indicating a significant difference significant (P0.05). Further tests using post hoc least significance different (LSD) showed a comparison of the average growth in lysed horse blood, lysed sheep blood, and lysed cow blood significantly different (P 0.05), while the average value of each treatment was the addition of lysed horse blood supplements, lysed sheep blood, and cow blood consecutive lysis was 8.551 ± 0.340, 7.117 ± 0.544, and 5.265 ± 0.548. The addition of lysed sheep blood and lysed cow blood to bolton broth media in this study can be used for C. jejuni isolation. However, the blood of lysed sheep had a higher effectiveness in the ability to grow C. jejuni, which was close to the ability to grow with the addition of lysed horse blood as a control, compared to the addition of lysed cow blood.

Blood is a good growth medium for bacteria by preventing the accumulation of toxic oxygen (peroxide and superoxide) and increasing the growth ability of these bacteria. The purpose of this study was to evaluate the ability of lysed sheep blood and lysed cow blood as a substitute for lysed horse blood on Campylobacter jejuni growth media, as well as evaluate the growth of C. jejuni from chicken meat samples on culture media supplemented with lysed sheep blood and lysed cow blood compared to the media cultures using lysed horse blood supplements. The concentration of lysed sheep blood, lysed cow blood, and lysed horse blood used was 5% each. The chicken meat samples used in this study were 30 samples. Based on the results, 14 samples of C. jejuni bacteria showed the ability to grow up to 107 and 16 samples up to 106. In the media added with lysed cow blood supplement only one sample was able to grow up to 106, 17 samples grew up to 105, and 12 samples grew up to 104. These results statistically used the one-way analysis of variance (ANOVA) test indicating a significant difference significant (P0.05). Further tests using post hoc least significance different (LSD) showed a comparison of the average growth in lysed horse blood, lysed sheep blood, and lysed cow blood significantly different (P 0.05), while the average value of each treatment was the addition of lysed horse blood supplements, lysed sheep blood, and cow blood consecutive lysis was 8.551 0.340, 7.117 0.544, and 5.265 0.548. The addition of lysed sheep blood and lysed cow blood to bolton broth media in this study can be used for C. jejuni isolation. However, the blood of lysed sheep had a higher effectiveness in the ability to grow C. jejuni, which was close to the ability to grow with the addition of lysed horse blood as a control, compared to the addition of lysed cow blood.

It is widely known that Escherichia coli (E. coli) can infect the gastrointestinal tract of various animals and damage some tissue organs. Through histopathological examination of the gastric and duodenal organs can provide valuable information relating to how the E. coli infection impacts the animal. On February 6th, 2023, euthanasia and necropsy of a dog belonging to Mr. Kamo Agil were carried out, which is located at Jl. Meyjend Sungkono, Gresik. Anamnesa is known to be a male dog, age ±1.5 months, body weight 0.6 kg. One kennel is filled with 9 dogs, dirty cage, in the house is not unused, damp and close to the warehouse area and furniture office. The dog has never been vaccinated and has not been dewormed. Temporary diagnosis of dogs with colibacillosis. Examination of pathology is carried out macroscopically and microscopically. Microbiological examination with isolation of escherichia coli bacteria and eosin methylene blue agar media. Followed by Gram staining and biochemical tests. The results of anatomical pathology examination showed that the dog had gastroenteritis. The results of microbiological examination showed that isolated and identified escherichia coli from duodenal organs. Based on the results of examinations showed that the dog had gastroenteritis caused by caused by escherichia coli infection.

It is widely known that Escherichia coli (E. coli) can infect the gastrointestinal tract of various animals and damage some tissue organs. Through histopathological examination of the gastric and duodenal organs can provide valuable information relating to how the E. coli infection impacts the animal. A male dog age 1.5 months, body weight 0.6 kg diagnosed with colibacillosis was euthanized. The dog has never been vaccinated and has not been dewormed. Examination of pathology is carried out macroscopically and microscopically. The standard of microbiological examination for E. coli isolation was conducted. Then, histopathological observation of gastric and duodenum organs was performed. The results showed that there were histopathological lesions in gastric and duodenum organs in dogs (canis familaris) suspected of E. coli infection

A cross-sectional study was conducted from December, 2019 to May, 2020 with the aim of determining seroprevalence and identifying the potential risk factors of brucellosis in dairy cows with recent cases of abortion and their owners and farm workers in selected sites in the central highland of Oromia, Ethiopia. The overall seroprevalence of bovine and human brucellosis was 0.61% (95%CI: 0.016–2.09) and 1.21% (95%CI: 0.032–4.27) using combined RBPT and CFT tests, respectively. The late stage of abortion (OR = 14.74, p =0.0002), retained fetal membrane (OR = 32.74, p=0.006), market-based stock replacement (OR = 16.55, p=0.002), and presence of parturition pen (OR = 11.511, p=0.027) were found to be significantly associated with seropositivity for Brucella infection in dairy cattle. Human housing (OR = 1.8, p = 0.002), contact with aborted fetus (OR = 21.19, p = 0.017), and drinking raw milk from aborted (OR = 5.72, 0.019) and retained fetal membrane (OR = 4.22, p= 0.029) cows all had a significant influence on human brucellosis seropositivity. A structured interview question was administered to 284 respondents. Accordingly, most respondents had no knowledge of bovine brucellosis and their zoonotic effects (93.3%) in contrast most of them drink raw milk. Implementation of a test and slaughter strategy with compensation is recommended with this low prevalence. In the case of human brucellosis, implementing one health approach framework should be practiced.

A cross-sectional study was conducted from December, 2019 to May, 2020 with the aim of determining seroprevalence and identifying the potential risk factors of brucellosis in dairy cows with recent cases of abortion and their owners and farm workers in selected sites in the central highland of Oromia, Ethiopia. The overall seroprevalence of bovine and human brucellosis was 0.61% (95%CI: 0.0162.09) and 1.21% (95%CI: 0.0324.27) using combined RBPT and CFT tests, respectively. The late stage of abortion (OR = 14.74, p =0.0002), retained fetal membrane (OR = 32.74, p=0.006), market-based stock replacement (OR = 16.55, p=0.002), and presence of parturition pen (OR = 11.511, p=0.027) were found to be significantly associated with seropositivity for Brucella infection in dairy cattle. Human housing (OR = 1.8, p = 0.002), contact with aborted fetus (OR = 21.19, p = 0.017), and drinking raw milk from aborted (OR = 5.72, 0.019) and retained fetal membrane (OR = 4.22, p= 0.029) cows all had a significant influence on human brucellosis seropositivity. A structured interview question was administered to 284 respondents. Accordingly, most respondents had no knowledge of bovine brucellosis and their zoonotic effects (93.3%) in contrast most of them drink raw milk. Implementation of a test and slaughter strategy with compensation is recommended with this low prevalence. In the case of human brucellosis, implementing one health approach framework should be practiced.

Feline lower urinary tract disease (FLUTD) is a complex patient condition caused by many factors. This case is the reason why pet owners always need veterinary assistance. Cats with urinary tract disorders show almost similar symptoms such as pollakiuria, hematuria, stranguria, urethral obstruction, and even urination outside the litter box. Urethral obstruction is a common complication in cases of FUS, which often occurs in male cats. Based on this, this study was conducted to determine the prevalence rate of FUS in the cat patient population at RSHP in 2022. The total number of patients treated at Veterinary Teaching Hospital (VTH) Prof. Dr. Noerjanto Faculty of Veterinary Medicine, Universitas Syiah Kuala was 849, and 38 cases of cat patients experiencied FLUTD. This research is an observational study to see the prevalence rate of FLUTD in a population of cat patients treated at the VTH. Based on the research that has been done, the prevalence value of animals experiencing FLUTD is 4.47. The number of cases of FLUTD in male cats is (97.3%) and for female cats (2.63%). The incidence of FLUTD that occurred in cats aged 2 to 4 years old.

Feline lower urinary tract disease (FLUTD) is a complex patient condition caused by many factors. This case is the reason why pet owners always need veterinary assistance. Cats with urinary tract disorders show almost similar symptoms such as pollakiuria, hematuria, stranguria, urethral obstruction, and even urination outside the litter box. Urethral obstruction is a common complication in cases of FUS, which often occurs in male cats. Based on this, this study was conducted to determine the prevalence rate of FUS in the cat patient population at RSHP in 2022. The total number of patients treated at Veterinary Teaching Hospital (VTH) Prof. Dr. Noerjanto Faculty of Veterinary Medicine, Universitas Syiah Kuala was 849, and 38 cases of cat patients experiencied FLUTD. This research is an observational study to see the prevalence rate of FLUTD in a population of cat patients treated at the VTH. Based on the research that has been done, the prevalence value of animals experiencing FLUTD is 4.47. The number of cases of FLUTD in male cats is (97.3%) and for female cats (2.63%). The incidence of FLUTD that occurred in cats aged 2 to 4 years old.