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Can the examination of different types of hive samples be a non-invasive method for detection and quantification of viruses in honey bee (Apis mellifera L.) colonies? Полный текст
2023
Čukanová Eliška | Prodělalová Jana | Palíková Miroslava | Kováčová Kristýna | Linhart Petr | Papežíková Ivana
Honey bee viruses have been shown to negatively affect the vigour and longevity of European honey bees (Apis mellifera L). In the present work, beehive materials were tested for their potential to serve as non-invasive samples for honey bee virus detection.
Показать больше [+] Меньше [-]Antimicrobial resistance of Listeria monocytogenes serogroups IIa and IVb from food and food-production environments in Poland Полный текст
2023
Lachtara Beata | Wieczorek Kinga | Osek Jacek
Listeria monocytogenes is an important foodborne pathogen responsible for human listeriosis, which is a disease with high hospitalisation and mortality rates. The bacteria are usually susceptible to most antibacterial substances, but resistance to some of them has been recently observed. The present study introduces the evidence on the emergence of antibiotic resistance among L. monocytogenes strains isolated from food and food-production environments in Poland.
Показать больше [+] Меньше [-]An initial characterisation of the Unfolded Protein Response pathway in haematopoietic canine cancer cell lines – a necessary step for the future development of new therapies in dogs with neoplasia Полный текст
2023
Hernández-Suárez Beatriz | Gillespie David A. | Obmińska-Mrukowicz Bożena | Pawlak Aleksandra
New and more effective therapies for canine cancer patients are urgently required and this necessitates advanced experimental research. Dogs are good models for studies in comparative oncology; however, canine cancer cell biology research is currently limited by low availability of validated antibody reagents and techniques. This study characterises the expression of key components of the unfolded protein response (UPR) in a panel of haematopoietic canine cancer cell lines using commercially available antibodies, and validates the methods used to study this pathway.
Показать больше [+] Меньше [-]Porcine carcasses as an underestimated source of antimicrobial resistant Campylobacter coli Полный текст
2023
Osek Jacek | Wieczorek Kinga
Campylobacteriosis is the most common human foodborne bacterial infection worldwide and is caused by bacteria of the Camplylobacter genus. The main source of these bacteria is poultry, but other food-producing animals such as pigs are also responsible for human infections. An increasing number of strains with resistance to fluoroquinolones and other antimicrobials such as macrolides were recently noted. The aim of the study was to investigate Campylobacter contamination of porcine carcasses and determine the antimicrobial resistance of the obtained isolates.
Показать больше [+] Меньше [-]Incidence and the risk of occurrence of benign and malignant canine skin tumours in Poland – a five-year retrospective study Полный текст
2023
Śmiech Anna | Bulak Kamila | Łopuszyński Wojciech | Puła Agata
The aim of the study was to compile data on the frequency and distribution of canine skin tumours and determine the risk of these being malignant as opposed to benign. This determination proceeded from tumour histogenesis and gave consideration to the dog’s breed, sex, age and the anatomical location of tumours.
Показать больше [+] Меньше [-]Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds Полный текст
2023
Beinhauerova Monika | Slana Iva
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a chronic infectious intestinal disease occurring in domestic and wild ruminants. Early diagnosis of infected herds enabling timely adoption of control measures is tremendously important in view of the fact that the disease has a significant economic impact on farmers. The aim of this study was to evaluate the possibility of rapid detection of viable MAP on small ruminant farms based on environmental sample examination using a novel phage-based test named Actiphage.
Показать больше [+] Меньше [-]Study on the expression of testin in the testes of dogs Полный текст
2023
Ciaputa Rafał | Nowak Marcin | Dzimira Stanisław | Brambilla Eleonora | Kandefer-Gola Małgorzata | Tomaszek Alicja | Popiel-Kopaczyk Aneta | Dzięgiel Piotr | Grieco Valeria
Testin is a protein involved in cell mobility, adhesion and colony formation. In rats, testin presence has been reported in the testes, and its possible role in spermatogenesis has been suggested. Studies in humans also suggest a possible role of testin as a cancer suppressor protein. In the dog, which represents both an important pet species and a good animal model for studying biological and pathological testicular processes, the presence of testin has never been reported.
Показать больше [+] Меньше [-]Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland Полный текст
2023
Smulski Sebastian | Pszczoła Marcin | Stachowiak Monika | Bilińska Adrianna | Szczerbal Izabela
MicroRNAs (miRNAs), a class of noncoding small RNAs, have been recognised as potential biomarkers of mammary gland conditions, including bovine mastitis diagnosis. The aim of this study was to quantify selected miRNAs in the milk of mastitic cows.
Показать больше [+] Меньше [-]Some aspects of the diagnosis and treatment of eosinophilic granuloma in cats Полный текст
2023
Omelchenko Hanna | Avramenko Natalia | Kulynych Serhii | Petrenko Maksym | Volosovets Vladyslav | Volosovets Natalia | Woźniakowski Grzegorz
Eosinophils represent the most active cells in mammals that show protective and assistive activity in the host immune defence against helminth parasites. These cells are also responsible for the reduction of allergic and inflammatory reactions. The eosinophils play a key role in allergic reactions by secretion of different chemical molecules leading to swelling, lesions and granuloma onset.
Показать больше [+] Меньше [-]Development of a recombinant protein-based ELISA for detection of antibodies against bovine herpesvirus 6 (BoHV6) Полный текст
2023
Kubiś Piotr | Kuźmak Jacek
Bovine herpesvirus 6 (BoHV6) belongs to the Herpesviridae family, Gammaherpesvirinae subfamily and Macavirus genus. It is common in cattle, but was also detected in American bison (Bison bison) and water buffalo (Bubalus bubalis). The aim of the experiment was to develop an ELISA for serological examination of cattle sera for the presence of anti-BoHV6 specific antibodies.
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