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Assessment of a bone biopsy technique for measuring tiludronate in horses: A preliminary study Полный текст
2011
Delguste, Catherine | Doucet, Michèle | Gabriel, Annick | Guyonnet, Jérôme | Lepage, Olivier M | Amory, Hélène
Assessment of a bone biopsy technique for measuring tiludronate in horses: A preliminary study Полный текст
2011
Delguste, Catherine | Doucet, Michèle | Gabriel, Annick | Guyonnet, Jérôme | Lepage, Olivier M | Amory, Hélène
peer reviewed
Показать больше [+] Меньше [-]Assessment of a bone biopsy technique for measuring tiludronate in horses: A preliminary study Полный текст
2011
Delguste, Catherine | Doucet, Michèle | Gabriel, Annick | Guyonnet, Jérôme | Lepage, Olivier M. | Amory, Hélène
This study assessed the feasibility of measuring tiludronate in horses using a minimally invasive bone biopsy technique. Eight horses were treated with intravenous (IV) tiludronate [1 mg/kg bodyweight (BW)], either once (n = 4) or twice, 28 d apart (n = 4). The horses that were treated once were euthanized on days 1, 43, 57, or 92 and those that were treated twice, were euthanized on days 112, 154, 194, or 364. Bone samples were taken bilaterally from each horse at 4 sites: the third metacarpal bone (MCIII), the 13th rib (R13), the tuber coxae (TC), and the cuboid bone (CB). Test samples were taken with a 5-mm diameter dental drill, while larger reference samples were taken with an osteotome. The concentrations of tiludronate were measured by high performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The TC was the easiest site to sample, and no technical difficulties were encountered for extraction and measurement. Drill sampling at the MCIII was difficult. Moreover, both the extraction and measurement caused technical problems and results were unreliable in most cases (93%). Drill samples obtained from the R13 were very small and access to the CB required considerable dissection, which would not be feasible in vivo. Forty-six percent and 36% of the tiludronate measurements performed on the R13 and CB samples, respectively, were unreliable. The ratio of tiludronate concentrations ranged from 73% to 185% (median: 118%) in the TC, 65% to 208% (median: 81%) in the R13, and 26% to 110% (median: 57%) in the CB. In all but 1 horse, the highest concentrations of tiludronate were found in the TC. It was concluded that bone biopsies performed at the TC were adequate for measuring tiludronate in horses and should be considered in future for repeated measurements over time in living animals.
Показать больше [+] Меньше [-]Impact of successive freezing-thawing cycles on 3-T magnetic resonance images of the digits of isolated equine limbs Полный текст
2011
Bolen, Géraldine | Haye, Dimitri | Dondelinger, Robert | Massart, Laurent | Busoni, Valeria
peer reviewed | The purpose of this study was to assess the impact of freezing and thawing on MR images of equine feet examined ex vivo. Nine equine cadaver digits were first imaged at room temperature (T0). Among the 9 digits, 3 (group 1) were imaged in a 3 Tesla MR system after one and after 2 freezing-thawing cycles. Digits of group 1 were thawed in a cold room at 4°C for 36h. Three other digits (group 2) were imaged after one freezing-thawing cycle. Digits of group 2 were thawed in a cold room at 4°C and then rescanned after 24h at room temperature. The last 3 digits (group 3) were scanned after one freezing-thawing cycle. Digits of group 3 were thawed at room temperature for 24h. Sequences used were Spin Echo (SE) T1, Turbo Spin Echo (TSE) T2 and proton density (PD), Short Tau Inversion Recovery (STIR), Double Echo Steady State (DESS), 3D Gradient Echo (GE) T1 and 2D GE T2*. Images obtained on the fresh limbs at room temperature were subjectively compared side by side to images obtained at the different freezing-thawing cycles. A quantitative analysis to assess signal change between examinations was realized by measuring signal to noise ratio (SNR). Visibility and margination of the anatomical structures of the foot and overall image quality were subjectively considered unchanged except for the hoof where the lamina was considered less visible distally after freezing and thawing in the GE T2* and in TSE T2 and PD sequences. Quantitative analysis demonstrated SNR changes in the bone marrow only in the distal phalanx in the SE T1 sequence when the feet were thawed at room temperature. When the feet were thawed in a cold room at 4°C, bone marrow SNR changes were present in the SE T1, GE T1 and TSE PD sequences. Signal changes were significant in the synovial recess when the thawing process was made at 4°C and not when the thawing process was at ambient temperature. The soft tissue structures and the hoof capsule showed significant changes with an increase of SNR, except in STIR, after freezing and thawing at 4°C and at room temperature. SNR changes in the soft tissues were mainly present in GE sequences.
Показать больше [+] Меньше [-]Horses under an enrichement program showed better welfare, strongler relationships with humans and less fear Полный текст
2011
Valenchon, Mathilde | Lévy, Frédéric | Neveux, Claire | Lansade, Léa | Physiologie de la reproduction et des comportements [Nouzilly] (PRC) ; Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) | Société Européenne d'Ethologie Vétérinaire des animaux Domestiques. Paris, FRA.
National audience
Показать больше [+] Меньше [-]The virulence of Brucella abortus isolated from cattle in Korea
2011
Lim, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, J.H., Northern Branch, Gyeongbuk Veterinary Service Laboratory, Andong, Republic of Korea | Kim, D.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, D.G., Gyeongsang National University, Jinju, Republic of Korea | Jun, M.H., Chungnam National University, Daejeon, Republic of Korea | Kim, S.H., Chungnam National University, Daejeon, Republic of Korea | Chang, H.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, H.J., Gyeongsang National University, Jinju, Republic of Korea | Min, W.G., Gyeongsang National University, Jinju, Republic of Korea | Kim, S., Gyeongsang National University, Jinju, Republic of Korea
In this study, we isolated 12 of Brucella (B.) spp. from cattle, which have been positive in Rose Bangal test and tube agglutination test in Gyeongbuk province in 2009. According to AMOS PCR analysis, isolated 12 strains were identified as B. abortus. Murine derived macrophage, RAW 264.7 cells, were infected with isolated 12 strains or reference strain (B. abortus 544), and bacterial internalization were characterized. According to these results, we divided the isolated strains into the following three groups: class Ⅰ, lower internalization than that of B. abortus 544; class Ⅱ, similar internalization to that of that of B. abortus 544; class Ⅲ, higher internalization than that of B. abortus 544 within RAW 264.7 cells. Furthermore, intracellular growth, bacterial adherent assay, LAMP-1 colocalization, virulence in mice and surface protein pattern were characterized. From these results, representative strains of class Ⅲ showed lower LAMP-1 colocalization, higher adherent efficiency, higher virulence in mice than those of B. abortus 544, and showed different pattern of surface proteins. These results suggest that B. abortus field strains, isolated from cattle in Korea, possess various virulence properties and higher internalization ability of field strain may have an important role for its virulence expression.
Показать больше [+] Меньше [-]Isolation and Antimicrobial Susceptibility of Microorganisms from Milk Samples of Jindo Dogs (Canis familiaris var. jindo)
2011
Lee, J.D., Jeollanamdo Livestock Sanitation Office, Gangjin, Republic of Korea | Lee, Y.K., Chonnam National University, Gwangju, Republic of Korea | O, S.I., Jindo Dog Research and Testing Center, Jindo, Republic of Korea | Jung, J.Y., Jeollanamdo Livestock Sanitation Office, Gangjin, Republic of Korea | Son, C.H., Chonnam National University, Gwangju, Republic of Korea | Shin, S.S., Chonnam National University, Gwangju, Republic of Korea | Oh, K.S., Chonnam National University, Gwangju, Republic of Korea | Hur, T.Y., National Institute of Animal Science, RDA, Cheonan, Republic of Korea | Suh, G.H., Chonnam National University, Gwangju, Republic of Korea
Lack of hygiene and puerperal mastitis are common causes of bacterial diseases in nursing neonates. The aim of this study was to isolate microorganisms from milk samples of healthy female Jindo dogs with suckling puppies and to investigate antimicrobial susceptibility against the isolated bacteria. Milk samples were collected from 120 udders of 12 lactating Jindo dogs that were 2~4 years old without any clinical diseases including mastitis. Bacteria were isolated from 64 milk samples (53.3%), either singly (76.6%) or in combination (23.4%). Staphylococcus (S.) spp. was the most common microorganisms (74.7%) isolated from canine milk, followed by Haemophillus spp. (10.9%), Streptococcus spp. (9.6%), Gardnerella spp. (2.4%) and Moraxella spp. (2.4%). The most frequently isolated organism was S. warneri (31.3%). Antimicrobial susceptibility of these bacteria was tested with 17 antimicrobial agents by Kirbyand Bauer standardized disc diffusion method. Results indicated that bacteria isolated from healthy canine milk were mostly susceptible to amoxicillin-clavulanic acid, cephalothin and ceftiofur, but were resistant to erythromycin, neomycin and tetracycline.
Показать больше [+] Меньше [-]Latex agglutination test based prevalence of Toxoplasma gondii in native Korean cattle
2011
Song, E.S., Chungnam National University, Daejeon, Republic of Korea | Jung, S.I., Chungnam National University, Daejeon, Republic of Korea | Park, B.K., Chungnam National University, Daejeon, Republic of Korea | You, M.J., Chonbuk National University, Jeonju, Republic of Korea | Kim, D.H., Chungnam National University, Daejeon, Republic of Korea | Song, K.H., Chungnam National University, Daejeon, Republic of Korea
The prevalence of Toxoplasma (T.) gondii was surveyed using a latex agglutination test (LAT) in native Korean cattle. A blood sample was collected from female 105 cattle in the Daejeon area of Korea. All cattle were asymptomatic and had not received any prophylactic treatment for T. gondii. Blood samples were collected via the caudal vein. The cattle ranged in age from 2~6 years (mean 3.7 years). LAT detected antibody to T. gondii in four of 105 (3.8%) cattle. However, the hazard analysis and critical control point protocol has been applied to cattle farms and beef traceability has been strengthen.
Показать больше [+] Меньше [-]Multiple congenital anomalies in a Korean native calf (Bos taurus coreanae)
2011
Islam, Mohammad Rafiqul, Chonbuk National University, Jeonju, Republic of Korea | Roh, Y.S., Chonbuk National University, Jeonju, Republic of Korea | Cho, A.R., Chonbuk National University, Jeonju, Republic of Korea | Park, H.J., Chonbuk National University, Jeonju, Republic of Korea | Heo, S.Y., Chonbuk National University, Jeonju, Republic of Korea | Lee, K.C., Chonbuk National University, Jeonju, Republic of Korea | Lee, H.K., Happy Veterinary Hospital, Kimje, Republic of Korea | Lim, C.W., Chonbuk National University, Jeonju, Republic of Korea | Kim, B.S., Chonbuk National University, Jeonju, Republic of Korea
Supernumerary ectopic limb(s) (SEL) is a congenital anomaly defined as the presence of accessory limb(s) attached to various body regions. This paper describes a case of SEL with ectopic lung and ectopia cordis in a newborn calf, based on macroscopic, microscopic and radiographic findings. External features of multiple congenital anomalies included an ectopic lung growing over the middle of the backbone and covered with normal haired skin. Ectopia cordis was found in the abdominal cavity and attached to the liver. Two extra abnormal limbs originated separately from within the ectopic lung. Most of the abdominal organs were exposed to the outside through the opened abdominal cavity. Microscopically the ectopic lung tissue had edema in the connective tissue around the bronchus and artery. Changes in other organs included congestion of the renal medulla, infiltration of inflammatory cells (lymphocytes and eosinophils) around the hepatic portal tract, and edema surrounding blood vessels and neurons in the brain. The rudimentary humerus of the forelimb was attached to the thoracic spine, as viewed radiographically. The hindlimb was consisted of an irregularly shaped femur, short tibia and fibula, two tarsal bones, one metatarsal bone, and three phalanges. This is the first description of congenital anomalies involving the SEL, ectopic lung and ectopia cordis in a calf.
Показать больше [+] Меньше [-]Eosinophilic granulomas in two dogs
2011
Kim, J.H., Jeju National University, Jeju, Republic of Korea | Jung, J.Y., Jeju National University, Jeju, Republic of Korea | Kang, S.C., Jeju National University, Jeju, Republic of Korea | Lee, Y.R., Gangnam Animal Medical Center, Busan, Republic of Korea | Lee, J.Y., Cats and Dogs Animal Clinic, Seoul, Republic of Korea | Hwang, E.K., Sangji University, Wonju, Republic of Korea | Woo, G.H., Semyung University, Jecheon, Republic of Korea | Kim, J.H., Jeju National University, Jeju, Republic of Korea
Eosinophilic granuloma is a common hypersensitive inflammatory skin disease in cats, and rare in dogs and horses. The skin biopsies of 5 years old female Cocker spaniel and 2 years old female mixed dog had the clinical signs of skin nodules with alopecia were submitted for diagnosis. Solitary skin nodules and papillary nodules were presented on the left external ear and back of Cocker spaniel and on the external ear of mixed dog, respectively. Histopathologically, epidermis of skin showed mild to severe hyperplasia with multifocal ulceration. Small to large irregular, brightly eosinophilic foci with degenerating eosinophils and homogeneous degenerated collagens were existed in the dermis of both ear and back skin. Typical 'flame figures', a mixture of degenerated collagen and degranulated eosinophils, were observed in both cases. Based on the histopathologic findings and special staining characters, 2 cases were diagnosed as canine eosinophilic granuloma. This is the first report for the eosinophilic granuloma of dogs in Korea.
Показать больше [+] Меньше [-]Therapy by traditional veterinary medicine in a case with canine fibrocartilaginous embolic myelopathy
2011
Park, H.J., Chungnam National University, Daejeon, Republic of Korea | Lim, S.J., Chungnam National University, Daejeon, Republic of Korea | Lee, S.H., Chungnam National University, Daejeon, Republic of Korea | Chung, D.W., Chungnam National University, Daejeon, Republic of Korea | Choi, J.H., Chungnam National University, Daejeon, Republic of Korea | Song, K.H., Chungnam National University, Daejeon, Republic of Korea | Lee, Y.W., Chungnam National University, Daejeon, Republic of Korea | Choi, H.J., Chungnam National University, Daejeon, Republic of Korea | Kim, D.H., Chungnam National University, Daejeon, Republic of Korea
A 6-year old, female, Schnauzer dog with chief complaint of tetraparesis was referred to the Veterinary Medicine Teaching Hospital of Chungnam National University. On physical examination, neulologic examinations and magnetic resonance imaging, this patient was diagnosed into fibrocartilaginous embolic myelopathy. Although conventional treatment such as prednisolone, cefotaxime and enrofloxacin revealed no improvement, this patient showed favorable therapeutic response by combined therapy with aquapuncture with prednisolone, modified moxibustion, herbal medicine and massage.
Показать больше [+] Меньше [-]A survey of traumatic reticular diseases in Korea and the effects on beef quality grade
2011
Byeon, H.S., Chungbuk Veterinary Service Laboratory, Cheongwon, Republic of Korea | Park, S.G., Chungbuk Veterinary Service Laboratory, Cheongwon, Republic of Korea | Lee, S.M., Chungbuk Veterinary Service Laboratory, Cheongwon, Republic of Korea | Quak, H.K., Chungbuk Veterinary Service Laboratory, Cheongwon, Republic of Korea | Kwon, K.M., Korea Institute for Animal Products Quality Evaluation, Gunpo, Republic of Korea | Ahn, B.W., Chungbuk National University, Cheongju, Republic of Korea
This study surveyed the prevalence of traumatic reticular diseases (TRD) of slaughter cattle in Korea, the typology of the causative foreign bodies and the effects on beef quality and carcass weight. The overall prevalence of TRD in 3,121 slaughter cattle was 5.5%. However, the prevalence was significantly higher in Korea indigenous cattle Hanwoo (5.8%, p less than 0.05) and female cattle (20.75%, p less than 0.001). The prevalence significantly increased in aged cattle (p less than 0.001). Major lesions related to foreign bodies were reticulitis (96%) and peritonitis (86%). Most causative foreign bodies were made of iron including nails, wires, steel rods, screw nails, and syringe needles. Cattle affected with TRD produced significantly lower grade quality of beef compared to normal cattle (p less than 0.0001), but TRD did not affect carcass weight. The data will be useful in the management of TRD, with the aim of increasing beef productivity in Korea.
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