Background: ARDS is a lung disorder that causes death in human and livestock and new therapeutic approaches such as stem cell therapy are essential because of lack of specific drug therapies for it. Objectives: Evaluation of the therapeutic effects of intrapulmonary transplantation of BM-MSCs to improvement of clinical signs in experimental model of ARDS created by lipopolysaccharide(LPS)E.Coli strains-O55:B5 in sheep is the aim of this study. Methods: In this study, 10 male sheep 3-4 months old Shall were used after random placement into two groups, treatment and control. Of sheep in the treatment group after anesthesia with ketamine and xylazine collected bone marrow samples and in the clean room BM-MSCs isolated, amplified and were identified with the evaluation of surface markers. Then experimental model of ARDS was induced by endotracheal injection of LPS to dose 400μg/kg. Clinical signs and radiograph images performed before and 24 hours after injection of LPS. After confirming inflammation, the sheep were anesthetized and on sternal position 50×106 cells of BM-MSCs third passage were transferred in treatment group as autograft by the catheter lavage in the bifurcation of the trachea and PBS in control group. Then clinical signs were recorded at hours of 3,6and 12 and on days 1,2,3 and 7 in both groups and finally, were analyzed based on the scored system. Results: The data showed transplantation of BM-MSCs caused significant improvement in clinical signs including heart rate, respiratory rate, body temperature, respiratory sounds, cough, mucosal status, nasal secretions, appetite and physical condition compared with control group. A significant decrease in respiratory rate and body temperature from 12 hours and in heart rate from 24 hours to next be began. Also, changes in breath sounds on the first day after transplantation, physical condition, mucous membranes and appetite on the third day, the occurrence of cough and abnormal discharge from the nose on the seventh day had returned to pre-inflammation (-24 time) and the median of score was zero for them. Conclusions: This study showed that transplantation of BM-MSCs can cause improves and the reduction in the severity of the clinical signs of ARDS, significantly.

Background: Hip dysplasia (HD) is an inherited, non-congenital disease that is particularly prevalent in large and giant breeds of dogs. The vast majority of dogs afflicted with HD show no clinical signs and it can be a highly debilitating condition for dogs. Objective: Aim of this study is a preliminary study on hip dysplasia based on clinical and radiographical examination in referral large breed dogs referred to veterinary teaching hospital of Ferdowsi University of Mashhad. Methods: Thirty six large breed dogs were examined for HD using Standard ventrodorsal hip extended view, and graded into five categories (A, B, C, D and E) using the FCI scoring system. PennHIP method was performed for measuring Distraction Index (DI). The Ortolani method was done to evaluate hip joint laxity. Results: HD was found in 72.2% of the dog, which included 11.1% unilateral and 61.1% bilateral. 73% of the afflicting cases were male. HD was reported In 11 dogs among 14 dogs with clinical signs and among 22 dogs without clinical signs 15 dogs were affected by HD. Ortolani test represented false negative in 33 cases and false positive in 6 cases. Conclusion: This study revealed the radiological evaluation of hip joint is essential in susceptible breeds for diagnosis and screening program of HD

BACKGROUND: Colostrum is the first milk produced after birth and is particularly rich in immunoglobulins, growth factors and antimicrobial peptides. Therefore, maintenance and storage of bovine colostrum has always been considered due to benefits of colostrum for the calf. Microbial contamination of colostrum is a concern because it is thought that bacteria in colostrum may interfere with passive absorption of colostral antibodies. OBJECTIVES: The aim of this study was to evaluate the effects of chemical preservatives and natural fermentation on quality and storage of colostrum. METHODS: Colostrum from the first milking of five Holstein cows was stored separately. Then colostrum samples were studied at days 1, 10, 20, and 30 of storage after treatment. All samples underwent microbiological culture for total plate count and detection of Escherichia coli, Coliforms, mold and yeast. Then whey was provided from untreated and treated samples at the end of 30th day and SRID (single radial immune diffusion) test was performed for assaying IgG antibody. Then the effect of treatments on colostrum antibody level was studied. RESULTS: The results showed that potassium sorbate did not have a role in reducing E.coli and coliforms count. However, it has prevented an increase in E.coli and coliform count during 30 days. Formic acid and propionic acid reduced the number of E. coli and coliform (p<0.05). In addition, the two organic acids promoted the growth of mold and yeasts compared with potassium sorbate. Nonetheless, the mentioned three treatments did not have an important role in reducing total count (p>0.05). The natural fermentation contributed to the decline of E. coli and coliform count while total count increased one day after treatment compared with other treatments, however among the treatments, formic acid and potassium sorbate were more effective than propionic acid and natural fermentation treatment for maintaining hygienic quality of colostrum. The result of measuring IgG antibody indicated that potassium sorbate has had more protective effect than other treatments. CONCLUSIONS: The results exposed that potassium sorbate and formic acid are better than other treatments to maintain colostrum quality with regard to increasing shelf life of colostrum.

BACKGROUND: The function of molecules of major histocompatibility complex (MHC) is to bind antigenic peptides and display them on the cell surface for recognition by T cells. MHC polymorphism is related to suseptibility or resistance to immune-mediated and infectious diseases. High Resolution Melt (HRM) analysis is a novel and powerful technique in molecular biology for the detection of genetic polymorphisms and can be used for discrimination of MHC alleles and genotyping. OBJECTIVES: Discrimination of dog MHC alleles (DLA-DRB1) by high-resolution melt analysis. METHODS: Forty blood samples were collected from dogs referred to Small Animal Teaching Hospital of faculty of veterinary medicine, University of Tehran. After DNA extraction and amplification the exon 2 of DLA-DRB1, HRM and Melt Curve analysis was carried out. Direct sequencing was used for checking the HRM patterns. RESULTS: Following the HRM and Melt curve analysis, forty samples could be classified into 8 genotypes (A to H). Most frequent was A (25.00%) and then C and E (each one 15.00%). In total, 82.5% and 17.5% of samples were heterozygote and homozygote, respectively. CONCLUSIONS: In this study, the HRM technique was used for DLA-DRB1 gene alleles typing for the first time. Confirmation HRM results through sequencing results revealed that HRM can be used for discrimination of DLA-DRB1 alleles.

Peritonitis, pericarditis and meningitis due to salmonella enterica in a Kermani ewe Summary : CASE HISTIRY : A Kermani ewe was examined because of inappetance and illthrifness. CLINICAL PRESENTATION : Clinical examination showed normal heart rate , tachy pnea, muffled heart sounds , stiff neck , dullness , dehydration , rumen atony and paled mucosal membrane . DIAGNOSITIC TESTING : Post mortem examination revealed pericarditis, peritonitis, intestinal adhesion, mesenteric thickness as well as meningeal thicknesses. Salmonella enterica was isolated in bacterial culture from affected tissues . ASSESSMENTS : Although there are some previous reports regarding the association between salmonella infection and peritonitis, pericarditis and meningitis in domestic animals, to the best of our knowledge, there is no previous report about the concurrent peritonitis, pericarditis and meningitis due to salmonella in ruminant . Key words : Peritonitis , Pericarditis , Meningitis , Salmonella , Sheep . . . . . .

BACKGROUND: Phytogenic products are considered as nonantibiotic feed additive which may exert their possible appreciate effects on poultry performance and health through stimulated digestive secretions. OBJECTIVES: This study was conducted to examine the effect of dietary Satureja khuzistanica powder (SKP) on pH of intestine contents, bile secretion and composition, and liver as well as blood lipids in Isfahan native breeder cocks. METHODS: Thirty-six 40-wk-old Isfahan native breeder cocks were individually caged and randomly allotted to three groups of 12 birds each and fed with diets containing 0 (control), 20 or 40 g/kg SKP for 10 weeks. At the end of experimental period all birds were killed and used for data collection on dry weight and pH of intestine segments, liver, bile and blood variables. RESULTS: Mean pH value in ceca contents was lower in the birds received diets containing 40 g/kg SKP compared with control cocks (P>0.05). Addition of SKP in diet at 20 g/kg significantly decreased liver lipids compared with the birds fed on control diet and the diet containing 40 g/ kg (P<0.05). Liver score based on external appraisal of the organ was improved in the cocks fed with SKP-supplemented diets compared with control birds (P <0.05). CONCLUSIONS: It was concluded that supplementation of diet with SKP could improve liver health and function in breeder cocks through reduced total lipids accumulation in liver. However, SKP does not exert cholinergic effect in cocks.

BACKGROUND: In aquaculture the fast growth, high feed efficiency and increased resistance against pathogens are favorable. One of the suitable approaches for improving the growth indices and resistance against pathogens is the application of probiotic bacteria. OBJECTIVES: The present study was conducted to evaluate the effect of nano/microencapsulated Lactobasillus bulgaricus by nano alginate/chitosan on the growth rate and feeding efficiency of great sturgeon (Huso huso) juveniles. METHODS: This experiment was conducted in a completely random design in four groups and group (5) with triplicate. 375 fish weighing (27.28±2.86 gr mean±SD), were randomly divided in to five equal groups in triplicates. The test groups were as follows: fish in T1 were fed with alginate/chitosan without bacterium, fish in T2 were fed with encapsulated bacteria with emulsification method, fish in T3 were fed with encapsulated bacteria with Ionicgelation method, fish in T4 were fed with only bacteria and control group. The fish were fed  3% of body weight per day. RESULTS: The results showed  the highest feed conversion ratio (FCR) was measured in T3 (1.64±0.11) and the lowest was observed in T4 (1.14±0.06).The highest values of SGR, PER, FER in T4 were seen among  the  treatments at days 30 and 60.                                                                                                                                             CONCLUSIONS: According the result, nanoalginat/Chithosan used for encapsulation of probiotics can be lead to decrease in growth performance compared to the other treatments.

BACKGROUND: Avian influenza virus H9N2 subtype and Ornithobacteriumrhinotracheale (ORT) causes histopathological lesions in birds. Yet, thereis not any study on tissue lesions caused by these pathogens co-infection inspecific pathogen free (SPF) chicks. OBJECTIVES: The aim of this studywas to evaluate the type, severity and extent of histopathological lesionsinduced by co-infection of H9N2 subtype avian influenza virus and Ornithobacteriumrhinotracheale in SPF chickens. METHODS: Sixty, one-day-old SPFchicks were divided randomly into three groups of twenty. At 21 days, thechicks in the first group were infected with H9N2 subtype AI virus and thesecond group was inoculated with H9N2 subtype AI virus and ORT simultaneously.The third group was inoculated with PBS as control. Then, three birds from eachgroup were randomly selected and euthanized and autopsied at 2  till 16 days post-inoculation (DPI). Thetissue samples were collected from trachea, lungs, liver, spleen, thymus, kidneys,cecal tonsil and bursa of fabricius. They were cut into 5 to 6 μm thicknesssections using paraffin embedding method and were stained by Hematoxylin andEosin (H&amp;E). RESULTS: The severity of histopathological lesions inthe second group was higher than first group. Tissue changes were not observedin control group. CONCLUSIONS: The results of this study showed thatinfected chickens with H9N2 subtype AI virus and ORT simultaneously causeexacerbated histopathological lesions compared to H9N2 subtype of AI virus

BACKGROUND: A clean bowel preparation prior to X-ray examination is essential to obtain an accurate diagnosis. OBJECTIVES: This study was performed to compare the effect of senna and polyethylene glycol on the bowel preparation of dogs before abdominal radiography. METHODS: Six adult healthy mongrel dogs from each sex ranging in age from 18 to 30 months received either 4 or 8 g/kg polyethylene glycol or 20 mg/kg senna through an orogastric tube 3 weeks apart. Food was withheld approximately 12 hours prior to drug administration. Radiography was performed 12 hours after bowel preparation. Only water was given ad libitum to animals during the day before radiography. Radiologist who was unaware of the method of bowel preparation, reviewed the standard radiographs and graded the feces and gas in the colon and rectum, fecal radio-opacity and quality of radiographs on a scale of 0 to 3 (0=inadequate to 3=excellent). Dogs were observed for any drugs side effects up to two weeks after administration of the laxatives. RESULTS: The cleanliness scores of colon for the 4 and 8 g/kg polyethylene glycol and senna groups were similar, 2.17±0.41. Also, the cleanliness scores of rectum for the 4 and 8 g/kg polyethylene glycol and senna groups were 2.50±0.55, 1.83±0.75 and 2.50±0.55, respectively. Both polyethylene glycol dosages and senna significantly improve bowel cleansing score in dogs (p<0.05). There were no significant differences between the groups with respect to colon cleansing scores, fecal radio-opacity and quality of radiographs (p≥0.05). 8 mg/kg polyethylene glycol significantly reduces feces impaction in rectum versus 4 mg/kg (p<0.05). There were no clinical differences between the two groups for any of the side effects. CONCLUSIONS: It is concluded that 8 mg/kg polyethylene glycol and senna are safe and effective methods of colon cleansing, well tolerated by dogs.

BACKGROUND: Betaine is a derivative of three methylates of glycine amino acids, found in the body of many animals. Objectives: The study was conducted to investigate the effects of betaine supplementation in the diet on gene expression and activities of lipogenic enzymes, and lipid levels of blood and liver in broilers. Methods: A total of 320 broilers were evaluated (Ross 308) in a completely randomized design with 4 treatments and 4 replicates, with the diets consisted of the control treatment which did not use the betaine supplementation. The second, third and fourth treatments contained 0.05, 0.08 and 0.11% betaine hydrochloride 98%, respectively. Results: Betaine supplementation to the diet has no significant effect on lipoprotein lipase gene expression, and the activities of enzyme Acetyl-CoA carboxylase and fatty acid synthase (p> 0.05). Betaine supplementation caused a significant decrease in liver cholesterol and triglyceride chicks fourth treatment (containing betaine hydrochloride 0.11 percent) compared to the control group (p<0.05). Conclusions: In general, the results show that in broilers lipoprotein lipase gene expression and the activities of enzyme Acetyl-CoA carboxylase and fatty acid synthase are less influenced by betaine.