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Особливості фіксації метеорологічних умов у сховищі даних кваліфікаційної експертизи сортів рослин
2021
Мельник, С. І | Лещук, Н. В | Орленко, Н. С | Стариченко, Є. М | Мажуга, К. М | Шкапенко, Є. А
Мета. Розробити багатовимірну модель сховища даних кваліфікаційної експертизи сортів рослин для фіксації метеорологічних умов у взаємозв’язку з фенологічними стадіями розвитку сортів, які проходять ВОС та ПСП експертизу. Методи. Для проведення досліджень з установленням основних структурних елементів багатовимірного сховища даних застосовано методи індукції, дедукції, аналізу та синтезу. У процесі проєктування сховища застосовано концепцію W. H. Inmon, адаптовану для сфери сільського господарства та аграрного бізнесу. Результати. Проаналізовано етапи кваліфікаційної експертизи сортів рослин та розглянуто методологічні підходи до створення багатовимірної моделі сховища даних. Висвітлено особливості застосування сховищ даних для збереження результатів кваліфікаційної експертизи сортів рослин на відмінність, однорідність та стабільність (ВОС) та придатність сорту до поширення (ПСП). Особливу увагу приділено реалізації взаємозв’язку результатів кваліфікаційної експертизи сортів рослин з даними метеорологічних спостережень на різних фенологічних етапах росту й розвитку рослин, відповідно до записів в електронному польовому журналі. Спроектовано даталогічну схему сховища даних та реалізовано її у середовищі MS SQL Server. Висновки. Визначено джерела надходження даних у сховища даних та реалізовано багатовимірну модель сховища даних за схемою «сніжинки». Подано схему сховища даних, яка забезпечує зв’язок між метеорологічними умовами проведення польових дослідів та результатними даними кваліфікаційної експертизи й налічує чотири таблиці вимірів. Для кожної таблиці вимірів та таблиці фактів визначено атрибутний склад даних. Сховище даних практично використовується для аналізу впливу погодних умов на показники ВОС та ПСП експертиз
Показать больше [+] Меньше [-]Уміст макро- та мікроелементів у рослинах роду Artemisia в умовах інтродукції у Національному ботанічному саду імені М. М. Гришка НАН України
2021
Корабльова, О. А | Рахметов, Д. Б | Шанайда, М. І | Вергун, О. М | Багацька, Т. С | Свиденко, Л. В | Іващенко, І. В
Мета. Визначити мінеральний склад рослин видів роду Artemisia (А. dracunculus L., A. abrotanum L. та A. argyi H.Lév. & Vaniot) для з’ясування можливості їх безпечного використання у харчовій та фармацевтичній галузях промисловості України. Методи. Рентгено-флуоресцентний метод визначення елементного складу рослинної сировини. Результати. Визначено вміст мінеральних елементів у рослинах роду Artemisia залежно від їхньої здатності поглинати елементи з ґрунту й накопичувати в коренях та органах наземної частини. Установлено кількісний уміст 21 макро- й мікроелемента, а також виявлено деякі особливості їхньої міграції та розподілу в системі ґрунт–корені–рослина. Зокрема, наземна частина досліджуваних рослин містить K, Fe, Cu, Zn та Mn, які є найважливішими елементами в житті рослинного організму. Досить високим є вміст мезоелементів Ca та S. Кількість токсичних елементів Pb, Sr і Zr у рослинах незначна. Елементи K і S накопичуються в наземній частині. Уміст елементів у наземній частині рослин за зменшенням їхньої концентрації можна представити у вигляді таких рядів: для Artemisia dracunculus – S> K> Ca> Cl> Fe> Sr> Zn> Mn> Cu> Zr> Rb> Br> Cr; для A. abrotanum – K> Ca> S> Cl> Fe> Zn> Sr> Mn> Cu> Br> Cr> Co> Zr> Rb> Ni; для A. argyi – K> Ca> S> Fe> Cl> Sr> Zn> Mn> Co> Zr> Cu> Rb> Br> Se. Уміст токсичних елементів у досліджуваних рослинах знаходився в межах гранично допустимих концентрацій для рослинної сировини та продуктів харчування. Висновки. Уперше в умовах інтродукції в Національному ботанічному саду ім. М. М. Гришка НАН України в рослинах Artemisia dracunculus, A. abrotanum та A. argyi визначено вміст макро- та мікроелементів, які безпосередньо пов’язані з метаболізмом біологічно активних сполук. З’ясовано особливості їхнього розподілу за органами рослин при переході з ґрунту в наземну масу. Отримані результати можуть бути використані для оцінювання та порівняння якості рослинної сировини представників роду Artemisia, з’ясування фармакологічних властивостей цих рослин, пов’язаних з деякими елементами мінерального складу, і використання їх у медичній та харчовій галузях. Отримані дані мають як наукове, так і практичне значення в доборі господарсько-цінних видів рослин для збагачення культигенної флори України.
Показать больше [+] Меньше [-]Differentiation and identification of winter bread wheat verieties according to a complex of baking quality indicators
2021
Демидов, О. А | Гудзенко, В. М | Правдзіва, І. В
Purpose. Reveal the features of the formation of a quality indicator complex in winter bread wheat depending on the growing seasons, preceding crops and sowing dates, as well as differentiate and identify genotypes with high and stable levels of manifestation. Methods. Field, laboratory, statistical. Results. A different share of the influence of the year conditions, the preceding crop, the sowing date and their interactions on the quality indicators of some varieties was determined. A different reaction of varieties in terms of quality indicators, depending on the investigated factors was revealed. The variation was very low for test weight, water absorption ability of flour, crumb porosity. Strong variation was observed for flour strength after sunflower and soybean as preceding crops, alveograph configuration ratio after sunflower and soybean, index of elasticity dough after corn, valorimetric value after mustard, dough dilution degree after green manure, sunflower, corn and especially after mustard and soybeans. The varieties, which on average for 2016/17–2018/19 reliably exceeded the standard both in individual indicators and in general in terms of physical indicators of grain and flour quality and dough rheological properties. GYT biplot analysis identified the genotypes ‘MIP Vidznaka’ and ‘MIP Assol’ with a more optimal combination of increased yield and a complex of quality indicators in terms of different years, preceding crops and sowing dates. Some varieties, namely, ‘Estafeta myronivs’ka’, ‘Trudivnytsia myronivs’ka’, ‘MIP Valensiia’, ‘MIP Yuvileina’, ‘Balada myronivs’ka’, ‘Vezha myronivs’ka’ were inferior to them, but were significantly superior the others. Conclusions. The selected by quality indicators varieties as genetic sources can be used in breeding process. A more stable level of yield and quality indicators at different sowing dates after different preceding crops should be expected for growing varieties ‘MIP Vidznaka’, ‘MIP Assol’, as well as ‘Estafeta myronivs’ka’, ‘Trudivnytsia myronivs’ka’, ‘MIP Valensiia’, ‘MIP Yuvileina’, ‘Balada myronivs’ka’, ‘Vezha myronivs’ka’. The peculiarities obtained in the research should be taken into account when evaluating and differentiating genotypes in breeding process, as well as developing basic elements of technology for growing the varieties of winter bread wheat.
Показать больше [+] Меньше [-]Effects of catnip (Nepeta cataria L.) and Mexican sunflower (Tithonia diversifolia L.) density on growth, yield, and proximate composition of jute mallow (Corchorus olitorius L.)
2021
Айоделе, О.П | Алуко, О.А | Адегбаджу, О. Д
Purpose. Jute mallow (Corchorus olitorius L.) is a mucilaginous vegetable and fiber crop cultivated in the tropics, where catnip (Nepeta cataria L.) and Mexican sunflower (Tithonia diversifolia L.) are common weeds. Hence, the study investigated the growth, yield, and nutrient level of jute mallow in weed-free, catnip, and Mexican sunflower environments. Methods. The study involved two screen-house experiments in a Completely Randomized Design (CRD) with six replications. The treatments were 0 (control), 2, 4, 6, 8, and 10 weed plants per pot in both experiments. These are 0, 100, 200, 300, 400, and 500 weed count per square meter equivalent, based on the surface area of the pots used. Mexican sunflower and catnip plants interacted with jute plants in the first and second experiments, respectively. Growth parameters of jute mallow were recorded weekly from 5 to 8 weeks after sowing (WAS), and harvesting was done at 8 WAS. The proximate composition of jute was evaluated using standard procedures outlined by AOAC. The data collected were subjected to analysis of variance (ANOVA), and means were separated using Duncan Multiple Range Test (DMRT) at P < 0.05. Results. Catnip and Mexican sunflower negatively impacted the morphological features of jute mallow from 100 plants per square meter upwards. Catnip and Mexican sunflower, at 300 and 100–500 plants per square meter, respectively, reduced the dry weight of jute mallow. The crude protein content of jute mallow was also lessened by Mexican sunflower at some point. Conclusions. The study recommends that the density of catnip and Mexican sunflower plants interacting with jute mallow should be maintained below 100 plants per square meter to prevent yield loss.
Показать больше [+] Меньше [-]Peculiarities of the classification of economically valuable indicators of highbush blueberry varieties of Vaccinium corymbosum L
2020
Мельник, С. І | Орленко, Н. С | Лещук, Н. В | Матус, В. М | Павлюк, В. А | Павлюк, Н. В
Purpose. To substantiate the features of the classification of morphobiological and economically valuable indicators of highbush blueberry varieties of Vaccinium corymbosum L. Methods. The studies were carried out during 2012–2019. Research methods: field (visual examination of the manifestation of morphological signs of vegetative and generative organs of blueberry plants), laboratory (determination of the content of vitamin C, total sugar, pectin, carotenes, anthocyanins, flavonoids, total acidity in berries), analytical, comparative, statistical (cluster analysis). Results. Over the years of research, varieties of highbush blueberries were characterized by a stable manifestation of homogeneous morphological traits and economically valuable performance indicators. In particular, the studied varieties are classified according to ripeness groups: very early: ‘Fiolent’; early: ‘Draper’, ‘Huron’, ‘Clockwork’, ‘Cargo’, ‘Laska’; medium: ‘Blue Ribbon’, ‘Top shelf’, ‘ZF08-070’, ‘Mavka’; late: ‘Liberti’, ‘Last Call’ and very late: ‘Aurora’, ‘Overtime’. It was determined that the quantitative morphological traits of highbush blueberry varieties that influenced the formation of plant productivity include such traits as growth vigor, leaf length, leaf width, inflorescences lengthwise, bunch density, fruit size. Economically valuable indicators of suitability of highbush blueberry varieties remained stable within the ripeness group. The highest yield (19.5 t/ha) was provided by the mid-season variety ‘Mavka’ of domestic selection. The yield of berries from the bush of this variety was also high and amounted to 7 kg. The studied performance indicators were low in the variety ‘Blue Ribbon’ – 10.2 t/ha and 2.4 kg, respectively. The minimum and maximum values of the manifestation of characteristics will serve to establish the limiting boundaries of its variation in the next modeling of economically valuable indicators of highbush blueberry varieties. Conclusions. Such morphobiological characteristics of highbush blueberry varieties, as plant growth vigor, leaf length, leaf width, inflorescences lengthwise, bunch density, fruit size are closely related to plant productivity, berry yield per bush, berry weight and yield. Therefore, they can be involved in the development and improvement of the model of suitability indicators of highbush blueberries for distribution in Ukraine.
Показать больше [+] Меньше [-]Features of the morphological structure of plants and biochemical properties of introduced and domestic varieties of Cannabis sativa L
2020
Полякова, А. С | Горшкова, Л. М | Рахметов, Д. Б
Purpose. Identifying the features of the seasonal growth rhythm, development of the plants, morphological and biological differences in monoecious introduced and domestic varieties of Cannabis sativa L. in the North-east region of Ukraine. Methods. The research was conducted from 2014 till 2019. The following research methods were employed: field (phenological observations, sampling for laboratory analysis), microscopic (study of the morphological structure of glandular and cystolithic hairs on vegetative and generative organs, determination of the size and density of hair formation), laboratory and analytical (determination of the content of cannabinoid substances by thin layer and gas-liquid chromatography), biomorphological methods (analysis of morphological characteristics of plants). Chemical analysis for the content of cannabinoid substances was carried out in the laboratory of the breeding and seed production department of the Institute of Bast Crops of the NAAS of Ukraine (Glukhiv, 2014–2019). The content of neutral substances [cannabinol (CBN), cannabidiol (CBD), tetrahydrocannabinol (THC)] and acids (CBDA, THCA) was determined by using a thin layer chromatography (TLC) and gas-liquid chromatography (GLC) methods. For the study, 11 monoecious varieties of C. sativa: ‘YuSO 1’, ‘YuSO 31’, ‘Hliana’, ‘Viktoriia’, ‘Hlesiia’, ‘Hlukhivskyi 46’, ‘Hlukhivski 51’, ‘Zolotoniski 15’ and 3 introduced varieties: ‘Fedora 17’, ‘Felina 32’, ‘Futura 77’ (France) which differed among themselves in morphological, biological, genetic, ecological-geographical and economically valuable characteristics were selected. Results. Biological and morphological characteristics of modern monoecious varieties of C. sativa were investigated depending on the ecological and geographical zones of origin, conditions of the growing season and the stage of ontogenesis. The functional interdependence between the formation of specialized excretory structures and the content of cannabinoid substances in plants of different varieties of C. sativa was revealed. The following promising varieties of C. sativa in a ration of ((THC + CBN) ≤ CBD) point the possibility to use them as the source material for creating the medicinal varieties of plants with a high content of CBD. Conclusions. Nowadays C. sativa is the main source of phyto material for industrial production of natural CBD. In recent years, high-yielding varieties of C. sativa have been created, which, together with high commercially valuable traits, did not contain psychoactive THC and had an insignificant amount of CBD. The results of the research indicated the features of the seasonal growth rhythm, development of the plants and the morphological and biological features of modern monoecious introduced and domestic varieties of C. sativa in the North-east region of Ukraine. The low correlation between the content of cannabinoid substances and their acids in varieties, which are characterized by the low content of cannabinoid compounds was established. According to the developed own classification (THC + KBN): CBD, varieties with a predominant CBD content ‘Hlukhivskyi 46’, ‘Zolotoniski 15’, ‘Hliana’ which are of significant medicinal value were identified.
Показать больше [+] Меньше [-]The results of divergence of early-maturing maize source material in heterosis breeding
2020
Черчель, В. Ю | Купар, Ю. Ю | Таганцова, М. М | Стасів, О. Ф
Purpose. To analyze the divergence of early maturing source material of corn Zea mays L. in heterosis breeding for the genetic base formation at the State Institution the Institute of Grain Crops of the NAAS of Ukraine. Methods. Field (comprehensive assessment of morphobiological and economically valuable characteristics of the source material and maize hybrids) individual selection, cumulative and recurrent selection, backross and testcrosses breeding methods; laboratory; analysis and synthesis; statistical. Results. The results of research on the analysis of the divergence of early maturing corn source material in the conditions of the Steppe of Ukraine has become a developed harmonized working collection of corn breeding samples adapted to the stressful conditions of this region. The gradual cyclical improvement of the lines made it possible to form the core of the genetic diversity of FAO 150–290 early maturing specimens of the southern ecotype, which are competitive in heterosis breeding. The complexity of breeding for early maturity in the steppe conditions is due to the lack of material adapted to the stress factors of the South of Ukraine. The available early maturing lines of the world collection F2, F7, Ер1, Ма21, Ма23, Со125, Со255, См7, PLS61, S72, etc., were distinguished by high cold resistance, good starting plant development, intensive accumulation of dry matter during ripening, but not adapted to the deficiency of moisture in the soil and high summer temperatures. According to the results of the experiment, it was revealed that, in terms of breeding, lines of Lancaster plasma (DK427 and DK633) were the most plastic, due to which a number of new mid-early lines were obtained, for example DK2/427, DK267, DK266/417, DK633/266, DK296, etc., which were included in the registered hybrids. Formation of the genetic base of early maturing maize source material for heterosis breeding and systematization according to different breeding characteristics provided a balance of samples of alternative components, which will be further used to model heterosis hybrids in the early maturing group. Conclusions. The updated basic collection of lines is represented by the samples of plasma Iodent: DK744SVZM, DK216SVZM, DK4173SVZM, DK235zS, DK257zM, SV, DK365SVZM, DK777ZMSV, DK733-7zM,SV, DK315SVZM; Lancaster: DK296zS,VM, DK633/266zS,VM, DK2965ZSZM, DK2953 ZSZM, DK3023 ZSZM, DK236zS,ZM; Raid (SSS): DK232MV, DK2323MV, DK239MV; Mixed: DK253ZSZM, DK273MV, DK272zS, DK281SV, DK233zM,SV, DK959MV, DK9527 ZSZM, DK247MV, DK2442MV, which is the basis of the genetic diversity of early ripening corn samples included in the State register of plant varieties suitable for dissemination in Ukraine.
Показать больше [+] Меньше [-]Peculiarities of leaf area formation and yield structure of ‘Antonina’ lentil variety depending on the elements of technology
2020
Присяжнюк, О. І | Слободянюк, С. В
Purpose. To reveal the peculiarities of leaf area formation and a structure of lentils variety ‘Antonina’ yield depending on the elements of cultivation technology: ino culation with nitrogen-fixing and phosphate-solubilizing microorganisms and the use of foliar growth stimulants. Methods. Field, laboratory. The experimental scheme included inoculation of seeds with nitrogen-fixing microorganisms (Ryzogumin), application of phosphate-solubili zing microorganisms (Polimixobacteryn and Biophosphoryn) into the row zone, and foliar feeding with a growth stimulator (Alga 600). Results. The results of studies of the leaf area formation peculiarities and the structure of lentils yield depending on the influence of cultivation technology elements are given in the article. It was found that the maximum indicators of the leaf area were formed by lentils in the flowering phase, which on average in the experiment was at the level of 37.5 thousand m2/ha, and in the control variant – only 32.0 thousand m2/ha. By inoculating the seeds with Rhyzogumin, applying phosphate-solubilizing biopreparation and foliar feeding, we obtained the maximum parameters of the leaf surface of lentil plants in the experiment. Thus, in the variant of inoculation with Rhyzogumin, application of Biophosphoryn and treatment with Alga 600 lentil plants formed a leaf area of 40.3 thousand m2/ha. However, due to the use of the phosphate-solubilizing biopreparation Polimixobacteryn and Alga 600 on the background of seeds inoculation with Rhyzogumin, the leaf area was formed at the level of 39.9 thousand m2/ha. Conclusions. When the seeds were treated with nitrogen-fixing microorganisms (Rhyzogumin) and phosphate-solubilizing bacteria (Polimixobacteryn and Biophosphoryn), the yield of lentils increased significantly. Thus, in the experimental plots in the variants with Ryzogumin + Polimixobacteryn the yield was – 1.64 t/ha, and in the combination Ryzogumin + Polimixobacteryn + growth stimulator Alga 600 the yield of lentils was – 1.90 t/ha. Seed inoculation had a positive effect on plant height. The best results were obtained when the seeds were treated with Ryzogumin in combination with Biophosphorin and Polimixobacteryn – 44.5 and 44.1 cm, respectively.
Показать больше [+] Меньше [-]Development of a method for rapid evaluation of the color of flour and pasta made from wheat of different species
2020
Вечерська, Л. А | Голік, О. В | Реліна, Л. І | Буряк, Л. І | Шелякіна, Т. А
Purpose. Develop a simplified method for estimating the color of flour and pasta using Adobe Photo Shop® software. Methods. The research material was grain of varieties and selection lines of wheat species Triticum durum, T. dicoccum, T. timopheevii bred in the Рlant Production Institute nd. a. V. Ya. Yuryev and samples of the National Center for Genetic Resources of Plants of Ukraine, 2018–2020 harvest. Batch of flour and pieces of dough were scanned using EPSON Scanner 010 F. Color was evaluated using Adobe PhotoShop®. The level of carotenoids was determined by spectrophotometric method. Results. Visual assessments of flour and pasta color, presented by different researchers, differ significantly (up to 2–3 points on a 9-point scale). At the same time, the use of the developed method of express color evaluation of flour and pasta samples in Adobe PhotoShop® with the color evaluation system L*a*b*, used in modern reflectometers, allows to obtain data in numerical value and provides high accuracy. In the case of the developed method, correlations were established between the content of carotenoids and the index b* of flour (r = 0.41 / ≤ 0.05) and pasta (r = 0.60 / p ≤ 0.001) and allowed to select the best in flour color selection lines 10-56, 10-65, 14-153, sample T. durum var. falcatomelanopus, in pasta color – lines 10-56, 10-65, 11-29, 12-3. Conclusions. The developed method provides high accuracy and can be used to evaluate the color of pasta and wheat flour, simplifies the evaluation of test samples, standardizes the parameters of agricultural products and avoids subjective judgment. The established weak correlation between the carotenoid content and the b* value of flour indicates the effect on the color of flour not only orange but also yellow and red pigments, and the average correlation between the carotenoid content and the b* value of pasta indicates the influence of the products of polyphenols oxidation reactions of wheat grain. The best in color flour and pasta lines were selected for breeding work to increase the content of carotenoid pigments in grain.
Показать больше [+] Меньше [-]Alleles of Ppd-1 genes that control sensitivity to photoperiod in a number of bread winter wheat genotypes
2020
Бакума, А. О | Чеботар, Г. О | Tkachuk, A. V. | Чеботар, С. В | Москалець, Т. З | Москалeць, В. В
Purpose. Analysis of the allelic state of Ppd-1 genes, which control sensitivity to photoperiod, in varieties and lines of bread winter wheat, and comparison of the results obtained with field observations on the duration of periods before heading and flowering, whose originators were the Nosivska Breeding and Research Station of the V. M. Remeslo Myronivka Institute of Wheat National Academy of Agrarian Sciences of Ukraine and Poltava State Agrarian Academy of the Ministry of Education and Science of Ukraine. Methods. The following methods were used in the work: DNA extraction, allele-specific PCR, agarose and polyacrylamide gel electrophoresis, analysis of variance. Results. It was revealed that ‘Yuvivata 60’ variety has a recessive Ppd-1 genotype and belongs to the III haplotype by a combination of mutations in the structure of Ppd-D1 gene. Line ‘L41/95’ was heterogeneous by alleles of Ppd-D1 gene, which corresponded to the presence of haplotypes III and VII. All other tested cultivars and lines were characterized by alleles Ppd-A1b, Ppd-B1b and Ppd-D1a, and assigned to haplotype VII. According to the results of statistical data processing, the duration of the period from May, 1 to heading was the smallest for the variety ‘Donskaya polukarlikovaya’ in the conditions of both the Forest-Steppe and Polissia-Forest-Steppe regions of Ukraine, the longest – in the varieties ‘Yuvivata 60’, ‘Myronivska 61’ and ‘L41/95’. The differences between these groups were significant and amounted to 10 days. Conclusions. A breeding material with a high adaptive ability for growing conditions in Polissia-Forest-Steppe zone was studied by the allelic state of the Ppd-1 genes. A low level of polymorphism in the studied varieties and lines was revealed by the alleles of Ppd-1 genes [12.5% – Ppd-D1b (²²²), 12.5% – Ppd-D1à/b (²²²/VII), 75% – Ppd-D1a (VII)], that agrees with the hypothesis that breeders gave a greater preference for the photoperiod-insensitive wheat genotype under Ukrainian conditions. The genotypes with the dominant Ppd-D1a (VII) gene almost completely dominate in the south of Ukraine. At the same time, in northern latitudes, weather conditions negate the advantages of the genotypes with Ppd-D1a gene
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