Purpose. Evaluate the genotype–environment interaction and identify the spring barley breeding lines with a combination of yield performance and stability in the multi-environment trials. Methods. Twelve barley breeding lines and standard variety ‘Vzirets’ were tested in three different ecological zones of Ukraine: Central Forest-Steppe, Polissia and Northern Steppe. To characterize the genotype–environment interaction and differentiate of breeding lines for yield and adaptability, a number of the most used methods were applied: S. A. Eberhart, W. A. Russel (1966); G. Wricke (1962); C. S. Lin, M. R. Binns (1988); M. Huehn (1990); A. V. Kilchevskiy, L. V. Khotyleva (1985); V. V. Khangildin, N. A. Litvinenko (1981); J. L. Purchase et al. (2000). Graphical analysis was performed with the AMMI model. Results. The high variation in the yield performance of spring barley breeding lines was revealed, which was determined both by the ecological and the weather conditions of the years of the research. The ANOVA revealed reliable contributions from all three source of the variation: genotype, environment and genotype–environment interaction. The part of influence for environment was the highest – 93.17%. The correlation between yield and individual stability indices was determined. Some indices estimated the stability only, without considering yield level. Other indices were related with the mean yield, with the maximum or minimum its limits. The breeding lines ‘Nutans 5152’, ‘Nutans 4982’, ‘Nutans 5069’ and ‘Nutans 5093’ with the optimal combination of yield performance and stability were identified. These breeding lines were transmitted to the Ukrainian Institute of Plant Varieties Examination for the qualification examination as new spring barley varieties ‘MIP Sharm’, ‘MIP Deviz’, ‘MIP Tytul’ and ‘MIP Zakhysnyk’, res­pectively. A number of breeding lines can be used in hybridization as a source of high adaptive potential for the suitable environmental conditions: Polissia – ‘Nutans 5061’, Polissia and Forest-Steppe – ‘Nutans 5081’ and ‘Nutans 4966’, Nor­thern Steppe – ‘Deficiens 5145’. Conclusions. Conducting multi-environment trials and processing experimental data in combination with statistical indices and AMMI promotes an in-depth assessment of the genotype–environment inte­raction and the identification the best of the best genotypes at the final stages of breeding process. | Мета. Оцінити взаємодію генотип–середовище та виділити селекційні лінії ячменю ярого з поєднанням потенціалу врожайності та стабільності в багатосередовищних випробуваннях. Методи. Дванадцять селекційних ліній ячменю ярого і стандарт сорт ‘Взірець’ досліджували у трьох різних екологічних зонах України: Центральний Лісостеп, Полісся та Північний Степ. Для характеристики взаємодії генотип–середовище та диференціації селекційних ліній за врожайністю і стабільністю використали низку найбільш поширених підходів: S. A. Eberhart, W. A. Russel (1966); G. Wricke (1962); C. S. Lin, M. R. Binns (1988); M. Huehn (1990); А. В. Кільчевський, Л. В. Хотильова (1985); В. В. Хангільдин, М. А. Литвиненко (1981); J. L. Purchaseetal. (2000). Графічний аналіз проводили з використанням AMMI моделі. Результати. Виявлена сильна мінливість врожайності селекційних ліній, яка зумовлювалась як екологічними, так і погодними умовами років досліджень. Дисперсійним аналізом виявлено достовірні вклади у варіацію генотипу, середовища та їх взаємодії. Частка умов середовища суттєво переважала інші – 93,17%. Визначена кореляція між врожайністю та окремими показниками стабільності. Низка параметрів оцінює тільки стабільність без урахування рівня врожайності. Інші параметри досить сильно пов’язані з середнім рівнем врожайності, або максимальним чи мінімальним її значенням. Виділені селекційні лінії ‘Нутанс 5152’, ‘Нутанс 4982’, ‘Нутанс 5069’ та ‘Нутанс 5093’ з оптимальнішим поєднанням потенціалу врожайності та стабільності. Дані лінії передані до Українського інституту експертизи сортів рослин для проведення кваліфікаційної експертизи як нові сорти ячменю ярого ‘МІП Шарм’, ‘МІП Девіз’, ‘МІП Титул’ та ‘МІП Захисник’, відповідно. Низка селекційних ліній може бути використана в гібридизації як джерело підвищеного адаптивного потенціалу для відповідних екологічних умов: Полісся – ‘Нутанс 5061’, Полісся та Лісостепу – ‘Нутанс 5081’ та ‘Нутанс 4966’, Північного Степу – ‘Дефіцієнс 5145’. Висновки. Проведення багатосередовищних екологічних випробувань та аналіз експериментальних даних поєднуючи статистичні показники і AMMI сприяє поглибленій оцінці взаємодії генотип–середовище та виділенню кращих з кращих генотипів на завершальних етапах селекційної роботи. | Цель. Оценить взаимодействие генотип–среда и выделить селекционные линии ячменя ярового с сочетанием потенциала урожайности и стабильности в многосредовых испытаниях. Методы. Двенадцать селекционных линий ячменя ярового и стандарт сорт ‘Взірець’ исследовали в трех различных экологических зонах Украины: Центральная Лесостепь, Полесье и Северная Степь. Для характеристики взаимодействия генотип–среда и дифференциации селекционных линий по урожайности и стабильности использовали ряд наиболее распространенных подходов: S. A. Eber­hart, W. A. Russel (1966); G. Wricke (1962); C. S. Lin, M. R. Binns (1988); M. Huehn (1990); А. В. Кильчевский, Л. В. Хотылёва (1985); В. В. Хангильдин, Н. А. Литвиненко (1981);J. L. Purchase et al. (2000). Графический анализ проводили с использованием AMMI модели. Результаты. Обнаружена сильная изменчивость урожайности селекционных линий, которая обусловливалась как экологическими, так и погодными условиями лет исследований. С помощью дисперсионного анализа обнаружены достоверные вклады в вариацию генотипа, среды и их взаимодействия. Доля условий среды существенно преобладала – 93,17%. Определена корреляция между урожайностью и показателями стабильности. Ряд параметров оценивает только стабильность без учета уровня урожайности. Другие параметры довольно сильно связаны со средним уровнем урожайности, максимальным или минимальным её значением. Выделены селекционные линии ‘Нутанс 5152’, ‘Нутанс 4982’, ‘Нутанс 5069’ и ‘Нутанс 5093’ с оптимальным сочетанием потенциала урожайности и стабильности. Данные линии переданы в Украинский институт экспертизы сортов растений для проведения квалификационной экспертизы как новые сорта ячменя ярового ‘МІП Шарм’, ‘МІП Девіз’, ‘МІП Титул’ и ‘МІП Захисник’ соответственно. Ряд селекционных линий могут быть использованы в гибридизации как источники повышенного адаптивного потенциала для соответствующих экологических условий: Полесье – ‘Нутанс 5061’, Полесья и Лесостепи – ‘Нутанс 5081’ и ‘Нутанс 4966’, Северной Степи – ‘Дефіцієнс 5145’. Выводы. Проведение многосредовых экологических испытаний и анализ экспериментальных данных в сочетании со статистическими показателями и AMMI способствуют углубленной оценке взаимодействия генотип–среда и выделению лучших из лучших генотипов на завершающих этапах селекционной работы.

Purpose. Provide bioinformatic analysis and comparison of target regions of the acetolactate synthase (als) gene in several members of the Poaceae family and, on the basis of the obtained data, explore the possibility of creating a unified genetic construct for als gene editing using the CRISPR-Cas9 system. Methods. The als gene sequences of various members of the Poaceae family were obtained from the NCBI: Nucleotide database. For comparison, a fragment of the imi-2 gene of wheat of the soft line ‘TealIMI11A’ was used in two regions of the 367–390 and 1729–1749 nucleotide sequences. The Sequence Viewer 3.37.0 tool was used to assess the presence of nucleotide substitutions in the working sequence of the imi-2 gene. The dendrogram was built using the “Blast Tree” tool from the NCBI: Blast: Nucleotide resource. Results. A comparative analysis of the nucleotide sequences of seven different species was carried out: soft wheat (Triticum aestivum L.), common wild oat (Avena fatua L.), barley (Hordeum vulgare L.), Asian rice (Oryza sativa L.), maize (Zea mays L.), aleppo grass (Sorghum halepense Pers.) and Tausch’s goatgrass (Aegilops tauschii Coss.). The dendrogram is based on the gene sequence als, showed that all studied genotypes can be divided into two blocks: the first block included maize and aleppo grass, and the second block, a separate branch includes Asian rice and common wild oat, barley, soft wheat and Tausch’s goatgrass. 367–390 nucleotide sequences of soft wheat showed the highest 100% homology to Asian rice, Tausch’s goatgrass and common wild oat. The lowest homo­logy was for maize and aleppo grass at 83.3%. Evaluation of the nucleotide sequence 1729–1749 showed no complete homology at the 100% level. It was the highest for barley and Tausch’s goatgrass – 95.2%, and the lowest for rice, maize and aleppo grass – 80.9% each. Conclusions. The analysis confirms a significant degree of homology of the als gene sequence for various species of the Poaceae family, which allows us to create a universal genetic vector. However, taking into account the high degree of sequence homology for species such as soft wheat, Tausch’s goatgrass, barley, Asian rice and common wild oat, it can be assumed that the corresponding genetic vector can be used with the greatest efficiency to alter the als gene of these genotypes.

The article provides progress report on the barley of F3к-10029/Saratovske 4 height decreasing throughout 1974 to 2012 by way of selecting plants of the shortest stem. 38 years of selecting the shortest stem genotypes cut down plant height by 5,7 times at the background of dominant Hl gene expression. Average plant height during 38 breeding cycles was descending by 2,69 cm, but this was not an even trend. New creative donor for ultimate short stem characteristic, Gnome 3, has been developed, with Hl-3Hl-3alleles designation. Relative impact on the efficacy of minus-selection by the plant height of the selection differential (38,00%) and inheritance coefficient in its narrow sense (14,56%) is established. Efficiency of the selection is realized with the decrease of winter rye height plants by 72,08% as expected by the relative breeding forecast. Analyzes is completed for 11 genetic and statistical clusters of average utilitarian characteristics of Gnome 3 ultra short stem rye over the period from 1974 to 2012.

Purpose. To develop a conceptually new method for determination of varietal purity (typicality), hybridity, steri­lity of seed lots. Methods of molecular biology (genomic DNA extraction, PCR with SSR markers application, capillary electrophoresis), genetic, statistical, mathematical analysis. Results. New method for determining the varietal qualities of seed lot was developed that consists of the following steps: simultaneous DNA extraction from a representative sample of aggregated seeds; PCR and further analysis of the amplification products by determination of the qualitative and quantitative composition of SSR-markers’ alleles; calculation of values of varietal seed lot quality using experimentally derived allele ratios. Conclusions. The developed method for determining varietal qualities of seed lots allows to reduce significantly the consumption of materials, time and labor during the analysis. Consistent qualification and quantification of alleles in the total sample of a seed lot is a conceptually new approach to establish varietal purity (typicality), hybridity, sterility.

Purpose. To analyze peculiarities of Ukrainian cereals and oilseeds trade, the situation on the world market, and determine future prospects of its development. Methods. Analysis and synthesis, comparative evaluation, graphic procedure. Results. The role and place of Ukraine in a total grain supply to the world market was determined. Ukraine is a world’s top ten grain producer. Among domestic agricultural products, cereals, oilseeds and sunflower oil are in the highest demand in the world. In recent years, our state has reinforced its status as one of the leading exporters of cereals. The commodity pattern of cereals and oilseeds export was analyzed with specifying most in-demand positions and the main countries purchasing these pro­ducts. According to the results of 2015, Ukraine obtained the highest foreign currency revenue from export of corn, wheat and barley (in grain structure) including soybeans and rapeseed (among oil crops). Key domestic and multinational operators are the main exporters of cereals and oilseeds in Ukraine and still hold their leading position. It was found a significant excess of import price of seeds as compared with export price of crops grown in Ukraine. Assortment of maize and sunflower seeds offered by major companies-producers in Ukraine was studied. Main trends of the world grain market development are considered. Conclusions. It was established that Ukraine is one of the major exporters of cereals and oilseeds. However, volatility of their prices significantly affects the export revenue that was decreasing even with increasing export quantities in kind. The dependence of domestic grain industry development on high-quality imported seed of maize and sunflower hybrids was recorded. It is expected that in the years to come Ukraine will maintain its strong positions in the world’s grain market.

Purpose. To develop a conceptually new method for determination of varietal purity (typicality), hybridity, steri­lity of seed lots. Methods of molecular biology (genomic DNA extraction, PCR with SSR markers application, capillary electrophoresis), genetic, statistical, mathematical analysis. Results. New method for determining the varietal qualities of seed lot was developed that consists of the following steps: simultaneous DNA extraction from a representative sample of aggregated seeds; PCR and further analysis of the amplification products by determination of the qualitative and quantitative composition of SSR-markers’ alleles; calculation of values of varietal seed lot quality using experimentally derived allele ratios. Conclusions. The developed method for determining varietal qualities of seed lots allows to reduce significantly the consumption of materials, time and labor during the analysis. Consistent qualification and quantification of alleles in the total sample of a seed lot is a conceptually new approach to establish varietal purity (typicality), hybridity, sterility.