Purpose. To study effects of low temperatures on Lavandula angustifolia plants and their response depending on their age and variety as well as identify critical temperature values for such structure elements of plants as bark, cambium, wood, pith. Methods. Field investigation, spectrometric analysis, statistical evaluation. Results. Topicality of investigation of Lavandula angustifolia to be grown in the Forest-Steppe zone of Ukraine was highlighted. Low temperature effects on structural elements of shoots for 8 varieties of domestic and foreign selection were analyzed. One-year shoots have suffered the most from low temperatures – 4.5 points on a six-point scale, two- and three-year shoots were damaged in a lesser degree – from 0.8 to 2 points. Plants of ‘Feuervogel’ and ‘Maestro’ varieties with total damage coefficient of 11.3% and 10.6% accordingly were the most resistant to low temperatures. These varieties can be recommended to producers for further introduction to the Forest-Steppe zone of Ukraine. Conclusions. Tissues of a lavender one-year shoot were most damaged by low temperatures regardless of a variety, but this organ is ephemeral and can be removed without causing damage to a plant. Two- and three-year shoots, which provide plant recovery, were significantly less damaged. None of the plants from 8 studied lavender varieties was lost under the influence of low temperatures, though some of them were damaged significantly. Such varieties as ‘Feuervogel’, ‘König Humbert’, ‘Veseli notky’ (pith damage is on the level of 1–2 points), ‘Maestro’ (0.8–2.5 points) were the most resistant to low temperature exposure which allows to conclude about successful lavender cultivation under conditions of the Forest-Steppe zone of Ukraine.