Purpose. To investigate rice blast resistance genes polymorphism by using bioinformatic methods. Methods. Global and local nucleotide alignment, phylogenetic analysis, HyPhy test. Results. For Pib gene, numerous single nucleotide substitutions and deletions of 1–3 bp were established. The phylo­geny of this gene has been studied and homologues have been found both in various rice species and in other cereals. These sequences can encode proteins that «recognize» the phytopathogens effectors, and can also be associated with resistance to phytopathogens. The Pi4 gene is characterized by single nucleotide substitutions, insertions and deletions; the number of non-synonymous substitutions exceeds the number of sy­nonymous ones. The Pi54 gene variability is significantly lower than that of the Pi4 and Pib genes. The predominant types of polymorphism were single nucleotide substitutions and small-sized indels. It was found that non-synonymous substitutions in Pi54, Pi4 and Pib genes were in close proximity, sometimes forming clusters, while some coding regions were either superconservative or contained predominantly synonymous substitutions. On philodendrograms, cultivated rice samples were clustered with samples of ancestral wild-growing species. Conclusions. Evolution of the rice blast resistance genes Pi4, Pib and Pi54 is characterized by diversification selection. Considering that tense coevolution and significant rate of adaptation and creation of new pathogen races are typical for a plant and a parasite, these genes are subjected to intensive selection aimed at increasing diversity for obtaining the resistance to new races of the pathogen.

It’s been shown the ways user impotent nucleotide plant of soybean, agriculture growing in the world last years. Revealed main point of formation nationality plant varieties resource of soybean.

Purpose. Investigation of maize ae1 gene polymorphism by bioinformatic methods. Methods. Global and local alignment of the nucleotide and amino acid sequences, in silico translation and transcription, translates modeling, pri­mers design, phylogenetic analysis. Results. 255 nucleotide sequences of maize аe1 gene, 500 amino acid sequences of homology translates of maize ae1 gene (SBEIIb enzyme homologs) and 100 mRNA expressed from the maize ae1 gene were analyzed to establish phylogenetic relationships. Polymorphism of maize ae1 gene different regions was investigated by bioinformatic methods. Modeling of the maize enzyme SBEIIb was performed. Conclusions. According to the results of amino acid sequences of SBEIIb enzyme homologs alignment, it was found that ae1 gene orthologs are pre­sent only in monocots, paralogs – in monocots, dicots, and other taxa, including algae and animals. Based on the results of alignment of plants mRNA from which enzyme SBEIIb is translated, maize ae1 gene orthologs and the nearest paralogs encoding starch branching enzymes with chloroplast localization were defined; this suggests a possible origin of ae1 gene due to duplication of the gene encoding the 1,4-alpha-glucan-branching enzyme 2 with chloroplast or amyloplast localization. In the maize ae1 gene structure, regions were found that include polymorphic sites not defined previously. For the polymorphic sites design primers were developed that allowed to differentiate the maize lines. It was determined that the detection of polymorphism in theory can influence the enzyme function and, as a result, change the concentration of amylopectin in maize grain.

Purpose. Investigation of maize ae1 gene polymorphism by bioinformatic methods. Methods. Global and local alignment of the nucleotide and amino acid sequences, in silico translation and transcription, translates modeling, pri­mers design, phylogenetic analysis. Results. 255 nucleotide sequences of maize аe1 gene, 500 amino acid sequences of homology translates of maize ae1 gene (SBEIIb enzyme homologs) and 100 mRNA expressed from the maize ae1 gene were analyzed to establish phylogenetic relationships. Polymorphism of maize ae1 gene different regions was investigated by bioinformatic methods. Modeling of the maize enzyme SBEIIb was performed. Conclusions. According to the results of amino acid sequences of SBEIIb enzyme homologs alignment, it was found that ae1 gene orthologs are pre­sent only in monocots, paralogs – in monocots, dicots, and other taxa, including algae and animals. Based on the results of alignment of plants mRNA from which enzyme SBEIIb is translated, maize ae1 gene orthologs and the nearest paralogs encoding starch branching enzymes with chloroplast localization were defined; this suggests a possible origin of ae1 gene due to duplication of the gene encoding the 1,4-alpha-glucan-branching enzyme 2 with chloroplast or amyloplast localization. In the maize ae1 gene structure, regions were found that include polymorphic sites not defined previously. For the polymorphic sites design primers were developed that allowed to differentiate the maize lines. It was determined that the detection of polymorphism in theory can influence the enzyme function and, as a result, change the concentration of amylopectin in maize grain.

Purpose. To evaluate hazelnut cultivars, species and hybrids from the genetic collection of Corylus spp. in the National Dendrological Park “Sofiyivka” of the NAS of Ukraine for the complex of economic characters. An attempt has been made to analyze the information on Corylus spp. identity, taxonomy and description, dissemination and ecological requirements of the species, possibilities to use the genetic potential for developing new cultivars. Methods. The value of the Corylus spp. collection representatives was investigated using conventional testing procedures. For summarizing information concerning phylogenetic reconstruction of the Corylus L. genus and hazelnut, a number of scientific publications to be proposed for discussion was analyzed. The oil content in hazelnut kernels and the fatty acid composition was determined using official methods. Results. The best samples of hazelnut genetic collection were included into the broad hybridization programme, and C. chinensis Franch. representatives as well. A number of hybrid seedlings was obtained including new hazelnut cultivars ‘Sofiyivsky 1’, ‘Sofiyivsky 2’ and ‘Sofiyivsky 15’ which were characterized by spherical or almost spherical fruits, high winter hardiness and drought resistance, as well as the absence of rhythmicity in fruiting. Conclusions. The collection of varieties, forms, cultivars and species of the Corylus L. genus created during the last years can be the base for hazelnut breeding in Ukraine.

Мета. Серед описаних у фаховій літературі ідентифікованих генів стійкості проти збудника бурої іржі виділити чужо­рідні, інтрогресовані у вид Triticum aestivum L. джерела, встановити їх походження та перспективи використання в селекційній практиці. Результати. Пшениця озима м’яка як основна зернова культура належить до групи рослин, яких найдавніше вирощують у контрольованих умовах. Протягом періоду вегетації вона зазнає згубного впливу збудників хвороб, тому пошук джерел стійкості проти них є першочерговим завданням селекції. Бура іржа – одна з найпоширеніших і шкодочинних хвороб пшениці. Вона призводить до значних втрат урожаю та погіршення якості зерна. Популяція збудника Puccinia reconditа вирізняється неабиякою адаптивною здатністю. Висока варіабельність вірулентності гриба призводить до накопичення патотипів, здатних долати гени стійкості пшениці. Найбільш екологічно безпечним методом контролювання захворювання є створення стійких сортів. Ефективність селекції на стійкість проти бурої іржі можна покращити, використовуючи різні Lr-гени стійкості. На цей час у геному пшениці та її родичів ідентифіковано й охарактеризовано за хромосомною локалізацією та ефективністю понад 90 (Lr) генів стійкості проти цього збудника. Виявлено, що майже всі ефективні на території України гени стійкості проти збудника бурої іржі, окрім Lr10 та Lr23, є чужорідними, перенесеними в Triticum aestivum від інших видів: Aegilops speltoides – гени Lr28, Lr35, Lr36, Lr47, Lr51, Lr66; Aegilops tauschii – Lr1, Lr21, Lr22а, Lr32, Lr39, Lr42; Triticum timopheevii – Lr18 та Lr50; Thinopyrum elongatum – Lr19, Lr29, Lr24; Secale cereale – Lr25, Lr26 та Lr45; Aegilops umbellulata – Lr9, Lr76; Triticum speltа – Lr44, Lr65, Lr71; Triticum dicoccoides – Lr53, Lr64; Aegilops triuncialis – Lr58, LrTr; Tr. timopheevii spp. viticulosum – LrTt1; Aegilops ventricosa – Lr37; Aegilops kotschyi – Lr54; Elymus trachycaulis – Lr55; Aegilops sharonensis – Lr56; Aegilops geniculate – Lr57; Aegilops peregrine – Lr59; Triticum turgidum – Lr61; Aegilops neglecta – Lr62; Triticum monococcum – Lr63. Висновки. Залучення до схрещувань культурних та диких видів родичів пшениці дасть змогу отримати неоднорідний за стійкістю проти збудника бурої іржі селекційний матеріал.