Purpose. To study morphoagrobiological and adaptive properties, level of yielding capacity of recently registered soft winter wheat varieties of various ecological groups under agroecological conditions of Kirovohrad variety testing station.Methods. Field study, laboratory test, analytical procedure and statistical evaluation.Results. It was established that the yield level of is a key composite indicator of genotype adaptation to agroecological growing conditions. Experimental data indicate significant deviations of yield depending on the genotype and the year of study. During three years of experiments, yield depending of the variety ranged from 4.26 to 9.71 t/ha, such varieties as ‘CN Kombin’, ‘Estivus’, ‘Tradytsiia odeska’, ‘Mudrist odeska’, ‘Lil’ and ‘Fabius’ had higher yields. In case of dry weather conditions and unfavorable agro-ecological factors, the following varieties as ‘Mudrist odeska’, ‘Veteran’, ‘Lil’, ‘Tsentylivka’, ‘Fabius’, ‘Patras’, ‘Montrei’ have demonstrated good adaptive properties. Their yield has decreased by 9,2–19,0%, while in the varieties ‘Mahistral’, ‘Poltavka’, ‘Harantiia odeska’ and ‘Pokrova’ – by 34.4, 42.4, 45.2 and 50.6% accordingly.Conclusions. Investigated soft winter wheat varieties differ in morphoagrobiological characteristics, productivity, height, maturation period, adaptability as well as economic and agronomic value. According to the complex of such indices as productivity, agronomic characters and properties as well as adaptability, in the microzone of Kirovohrad variety testing station it is advisable to grow varie­ties ‘CN Kombi’, ‘Pokrova’, ‘Mudrist odeska’, ‘Veteran’ and ‘Lil’.

Purpose. Develop a simplified method for estimating the color of flour and pasta using Adobe Photo Shop® software. Methods. The research material was grain of varieties and selection lines of wheat species Triticum durum, T. dicoccum, T. timopheevii bred in the Рlant Production Institute nd. a. V. Ya. Yuryev and samples of the National Center for Genetic Resources of Plants of Ukraine, 2018–2020 harvest. Batch of flour and pieces of dough were scanned using EPSON Scanner 010 F. Color was evaluated using Adobe PhotoShop®. The level of carotenoids was determined by spectrophotometric method. Results. Visual assessments of flour and pasta color, presented by different researchers, differ significantly (up to 2–3 points on a 9-point scale). At the same time, the use of the developed method of express color evaluation of flour and pasta samples in Adobe PhotoShop® with the color evaluation system L*a*b*, used in modern reflectometers, allows to obtain data in numerical value and provides high accuracy. In the case of the developed me­thod, correlations were established between the content of carotenoids and the index b* of flour (r = 0.41 / ≤ 0.05) and pasta (r = 0.60 / p ≤ 0.001) and allowed to select the best in flour color selection lines 10-56, 10-65, 14-153, sample T. durum var. falcatomelanopus, in pasta color – lines 10-56, 10-65, 11-29, 12-3. Conclusions. The developed method provides high accuracy and can be used to evaluate the color of pasta and wheat flour, simplifies the evaluation of test samples, standardizes the parameters of agricultural products and avoids subjective judgment. The established weak correlation between the carotenoid content and the b* value of flour indicates the effect on the color of flour not only orange but also yellow and red pigments, and the ave­rage correlation between the carotenoid content and the b* value of pasta indicates the influence of the products of polyphenols oxidation reactions of wheat grain. The best in color flour and pasta lines were selected for breeding work to increase the content of carotenoid pigments in grain.

Purpose. Study of winter wheat varieties that were bred at the Institute of Irrigated Farming NAAS through ecological testing in Turkey. Test procedures both in Ukraine and Turkey meet the requirements of the International Union for the Protection of New Varieties of Plants (UPOV). Methods. Field, laboratory ones: structural analysis, quality analysis, statistical analysis. Results. The Exporter Unions Seed and Research Company (ITAS) during 2011–2013 tested winter wheat varie­ties bred at the Institute of Irrigates Farming (IIF) NAAS at four areas of the Central Plateau of Turkey comparing them with local varieties registered in this country. According to the results of these tests the Exporter Unions Seed and Research Company identified Kohana variety as candidate for the state registration in Turkey. Conclusions. The realisation of the innovative product in Ukraine and entitlement to use intellectual property due to licensing agreements is expected. The Institute of Irrigated Farming NAAS continues to create new varieties of winter wheat which can be transferred to other countries for testing for the purpose of introduction and further expansion.

State and prospects of growing for Triticale crop at the territory of leading states of the world are highlighted across space and time. Observing the trend of changes in planting acreage and the product yield of wheat and rye hybrids, their popularity in agrarian and industrial fields of a whole range of countries in Asia, Northern and Southern America, Australia, a Ukrainian manufacturer is invited to consider and reasonably assess this crop and thus list in the composition of planted acreage the national nowadays varieties of triticale as an essential or strategic system component.

Unique features of Myronivs’ka 808 variety have provided its triumph in agricultural production in the former Soviet Union and other countries. Myronivs’ka 808 has become one of the most popular varieties in terms of sown areas (in 1971 over 9.5 million hectares) in the world production. Up to now the variety has been cultivated in the Russian Federation and the Republic of Kazakhstan. Myronivs’ka 808 variety gained leadership in the global selection process as a source of high grain quality, high productivity, general adaptability and plasticity. Using germplasm of this variety enabled to develop over 400 wheat varieties including 350 winter varieties and 60 spring varieties.

Dynamics of technological and sowing indexes of grain of wheat winter-annual kinds of Natsionalna, Stolichna, Artemis in the course of long-term storage is studied. It is positioned that the best sowing indexes throughout all shelf-life the kind of Stolichna, and technological had a kind of Natsionalna. The optimum shelf-life of grain of investigated kinds has compounded six months.

The study of vernalization requierement of winter bread wheat 87 modem varieties from Ukraine and Russia showed significant domination - 81.6% - of varieties with short vernalization requierement (30-40 days). Vernalization requierement differences among varieties from different climatic zones were revealed.

Purpose. Reveal the features of the formation of a quali­ty indicator complex in winter bread wheat depending on the growing seasons, preceding crops and sowing dates, as well as differentiate and identify genotypes with high and stable levels of manifestation. Methods. Field, laboratory, statistical. Results. A different share of the influence of the year conditions, the preceding crop, the sowing date and their interactions on the quality indicators of some varie­ties was determined. A different reaction of varieties in terms of quality indicators, depending on the investigated factors was revealed. The variation was very low for test weight, water absorption ability of flour, crumb porosity. Strong variation was observed for flour strength after sunflower and soybean as preceding crops, alveograph configuration ratio after sunflower and soybean, index of elasticity dough after corn, valorimetric value after mustard, dough dilution degree after green manure, sunflower, corn and especially after mustard and soybeans. The varieties, which on average for 2016/17–2018/19 reliably exceeded the standard both in individual indicators and in general in terms of physical indicators of grain and flour quality and dough rheological properties. GYT biplot analysis identified the genotypes ‘MIP Vidznaka’ and ‘MIP Assol’ with a more optimal combination of increased yield and a complex of quality indicators in terms of different years, preceding crops and sowing dates. Some varieties, namely, ‘Estafeta myronivs’ka’, ‘Trudiv­nytsia myronivs’ka’, ‘MIP Valensiia’, ‘MIP Yuvileina’, ‘Balada myronivs’ka’, ‘Vezha myronivs’ka’ were inferior to them, but were significantly superior the others. Conclusions. The selected by quality indicators varieties as genetic sources can be used in breeding process. A more stable level of yield and quality indicators at different sowing dates after different preceding crops should be expected for growing varieties ‘MIP Vidznaka’, ‘MIP Assol’, as well as ‘Estafeta myronivs’ka’, ‘Trudivnytsia myronivs’ka’, ‘MIP Valensiia’, ‘MIP Yuvileina’, ‘Balada myronivs’ka’, ‘Vezha myronivs’ka’. The peculiarities obtained in the research should be taken into account when evaluating and differentiating genotypes in breeding process, as well as developing basic elements of technology for growing the varieties of winter bread wheat.

Purpose. Provide bioinformatic analysis and comparison of target regions of the acetolactate synthase (als) gene in several members of the Poaceae family and, on the basis of the obtained data, explore the possibility of creating a unified genetic construct for als gene editing using the CRISPR-Cas9 system. Methods. The als gene sequences of various members of the Poaceae family were obtained from the NCBI: Nucleotide database. For comparison, a fragment of the imi-2 gene of wheat of the soft line ‘TealIMI11A’ was used in two regions of the 367–390 and 1729–1749 nucleotide sequences. The Sequence Viewer 3.37.0 tool was used to assess the presence of nucleotide substitutions in the working sequence of the imi-2 gene. The dendrogram was built using the “Blast Tree” tool from the NCBI: Blast: Nucleotide resource. Results. A comparative analysis of the nucleotide sequences of seven different species was carried out: soft wheat (Triticum aestivum L.), common wild oat (Avena fatua L.), barley (Hordeum vulgare L.), Asian rice (Oryza sativa L.), maize (Zea mays L.), aleppo grass (Sorghum halepense Pers.) and Tausch’s goatgrass (Aegilops tauschii Coss.). The dendrogram is based on the gene sequence als, showed that all studied genotypes can be divided into two blocks: the first block included maize and aleppo grass, and the second block, a separate branch includes Asian rice and common wild oat, barley, soft wheat and Tausch’s goatgrass. 367–390 nucleotide sequences of soft wheat showed the highest 100% homology to Asian rice, Tausch’s goatgrass and common wild oat. The lowest homo­logy was for maize and aleppo grass at 83.3%. Evaluation of the nucleotide sequence 1729–1749 showed no complete homology at the 100% level. It was the highest for barley and Tausch’s goatgrass – 95.2%, and the lowest for rice, maize and aleppo grass – 80.9% each. Conclusions. The analysis confirms a significant degree of homology of the als gene sequence for various species of the Poaceae family, which allows us to create a universal genetic vector. However, taking into account the high degree of sequence homology for species such as soft wheat, Tausch’s goatgrass, barley, Asian rice and common wild oat, it can be assumed that the corresponding genetic vector can be used with the greatest efficiency to alter the als gene of these genotypes.

Purpose. To reveal breeding and genetic peculiarities of modern spring barley varieties for the “number of grains per main ear” trait and identify genetic sources of increased combining ability for involving in hybridization. Methods. Investigations were carried out at the V. M. Remeslo Myronivka Institute of Wheat of NAAS of Ukraine. Modern varieties of domestic (‘Virazh’, ‘Talisman Myronivskyi’, ‘Komandor’) and foreign (‘KWS Aliciana’, ‘KWS Bambina’, ‘Zhana’, ‘Explorer’) breeding were involved in crossing for a full (7´7) diallel scheme. Parents and F1 were studied in field conditions during 2014–2016. Results. The analysis of variance of combining ability has shown a significant advantage in varying of general combining ability (GCA) effects. The mean square of specific combining ability (SCA) was significantly less than the GCA, but reliable throughout the years. The reciprocal effect was reliable only in 2014. Stably high effects of GCA during all years of investigations were noted in the varieties ‘KWS Aliciana’ (1.18–1.62) and ‘Virazh’ (1.33–1.48). The variety ‘KWS Bambina’ was characterized by lower but reliable positive effects of GCA (0.43–0.99) as compared to mentioned above. Non-allelic gene interaction was not found, that allowed to calculate the basic parameters of genetic variation. During all years of investigations, dominant effects of genes (H1 and H2) prevailed over the additive (D) ones in phenotypic expression of grain number per main ear. Mean degree of dominance in the experiment (H1/D) has shown overdominance. The same pattern was also distinctive for the index of mean degree of dominance in the loci . The dominance was reliably directed. Dominant effects of genes increased grain content, and recessive ones reduced it. At least 3–4 genes (groups of genes) have been revealed which determined the effects of dominance. At the same time, recessive genes (F<0) or gene effects were prevailed quantitatively in the varieties investigated. A high coefficient of heritability in broad sense (H2 = 0.98) has shown a significant determination of phenotypic variability with genetic factors. The coefficient of heritability in narrow sense (h2 = 0.66–0.68) confirmed that despite the advantage of dominant effects over the additive ones, the contribution of the latter was also significant. Conclusions. The prevalence of dominant effects of genes in the phenotypic expression of the number of grains per main ear causes the need for sufficient sample size of hybrid material and points to the expediency of conducting a more “rigid” selection for phenotype in later generations. At the same time, the considerable contribution of additive effects and high values of heritability indices give reason to predict the efficiency of selections aimed at increasing the trait in created hybrid material. The varieties ‘Virazh’, ‘KWS Aliciana’, ‘KWS Bambina’ should be used as effective genetic sources to increase grain content in combination breeding.