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Biological peculiarities of stem softwood cuttings of ornamental garden plants and their use in landscaping
2016
Т. В. Мамчур
Purpose. To study biological peculiarities of softwood cuttings of three species of ornamental garden plants of the Hydrangeaceae family introduced under the conditions of a botanical nursery of Uman National University of Horticulture, and the prospects of planting material use in landscaping. Methods. Field study, laboratory test, statistical and mathematical analysis. Results. Bioecological and ornamental peculiarities of grafters of investigated species and their preservation for supplying the store of initial plant graft material were studied. The dependence of regeneration ability of softwood cuttings of investigated varieties on preplant treatment by a biologically active substance (KANO), type of a shoot, time of grafting, etc was analyzed. Substantially intensive rooting of basal drafts with three nods and less intensive rooting of apical drafts was observed during the whole period of rooting. Conclusions. The treatment of softwood cuttings with a biologically active substance at the concentration of 10–15 mg/l in the aqueous solution stimulates root formation and activates growth of own root plants. Investigated plant species of Hydrangeaсeae family are promising for landscaping on urbanized areas of various functional use.
Показать больше [+] Меньше [-]Promising new varieties in food corn assortment
2016
Харченко, Ю. В | Харченко, Л. Я | Тимчук, С. М | Поздняков, В. В | Кузьмишина, Н. В
Purpose. Reporting peculiarities of new forms of edible corn and prospects for their use in food production that have been created jointly by scientists from the Plant Production Institute nd. a. V. Ya. Yuryev of NAAS and Ustymivka Experimental Station for Plant Production on the base of the environmentally friendly method that uses biochemical effects of natural mutations of corn grain endosperm structure. Methods. Field study, laboratory analysis, mathematical and statistical evaluation. Results. Original series of inbred lines as well as promising varieties and hybrids of sugar and waxy corns were obtained. Varieties and hybrids are universally usable for obtaining both fresh and canned products, characterized by good taste, provide yields of up to 10 tons per hectare of commercial products and aimed to grow in various soil and climatic conditions. Brief economic and morphological characteristics of corn varieties and hybrids created jointly by scientists from the Plant Production Institute nd. a. V. Ya. Yuryev of NAAS and Ustymivka Experimental Station for Plant Production were given. Conclusions. The use of biochemical effect of natural mutations of endosperm structure is cost-effective and environmentally friendly method for creating lines, varieties and hybrids of corn for direct consumption and processing into food products. ‘Biliavka’, ‘Nika’ varieties and ‘Binom F1’, ‘Solo F1’, ‘Twister F1’ hybrids created with the help of this method are promising for commercial use
Показать больше [+] Меньше [-]Development of multiplex PCR system for identification of glyphosate-tolerant sugar beet
2016
Присяжнюк, Л. М | Шитікова, Ю. В | Волчков, О. О
Purpose. To create a multiplex system for identification glyphosate-tolerant sugar beet by using PCR. Methods. Molecular genetic analysis. Results. The article presents the results of studies to determine the parameters of the polymerase chain reaction (PCR) in order to develop a multiplex system for identification of the structural elements of the design of transgenic gene cp 4 epsps, which provides tolerance to glyphosate. For amplicon target DNA sequences, the following values of temperature conditions of PCR were determined: step 1 (initial denaturation) 95 °C – 3 min; step 2 (specific reaction products accumulation): denaturation 95 °C – 45 s; hybridization of primers 55 °C – 50 s; elongation 72 °C – 1 min; number of cycles – 40; step 3 (final elongation) 72 °C – 6 min. A series of PCR were carried out for the purpose of selecting the optimal amount of DNA matrix for efficient estimate of transgenic sugar beet plants for the presence of specific sequences. Conclusions. To identify transgenic glyphosate-tolerant sugar beet, it is advisable to determine 35S promoter and gene cp 4 epsps in individual genotypes. It was found that during the selection of temperature parameters of multiplex reaction a 5 °C rise in primer hybridization temperature did not affect the identification of gene als that allowed to include specific primers for determination of this sequence as an internal control. Based on the results of test multiplex reactions, concentrations of dNTPs and Mg2+ ions were determined that allowed to exclude the possibility of non-specific fragments and false-negative results. The optimum amount of matrix DNA (100–150 ng) for an efficient estimate of transgenic sugar beet plants for the presence of specific sequences was determined. Obtained results allowed to develop a multiplex test system for identification of transgenic glyphosate-tolerant sugar beet which can be used for simultaneous determination of the 35S promoter, cp 4 epsps gene and als gene as an internal reaction control.
Показать больше [+] Меньше [-]Evaluation of pea varieties based on correlation of quantitative traits and indices
2016
Присяжнюк, О. І | Король, Л. В
Purpose. To evaluate pea plants productivity and determine the degree of correlation among the main agronomic characters in pea varieties. Methods. Structural and statistical analysis. Results. Evaluation of pea samples in terms of indices of plant productivity elements level based on correlation analysis and single-factor indices appliance provided insight into the ratio of one trait share per unit of another one. It was defined that some correlations among the elements of productivity was not only moderate and weak, but they also changed their sign that could be the evidence of growth conditions influence on structural relationships between some traits and, consequently, redistribution of their contributions to the formation of variety productivity. Positive and very close relationship of many traits was revealed, particularly between plant height and the height of the plant up to the first bean, the number of nodes and the number of sterile nodes (r = 0,95–0,97). Methodological aspects of the variety model creation were considered, that may be useful not only in pea breeding but also for improving the technology of its cultivation. Conclusions. Correlation relationships were established between the number of beans and the number of fruiting nodes and the number of carpophores containing 2 beans (r = 0,86–0,88), seed mass and plant mass (r = 0,81), the number of seeds per plant and plant mass and seed mass per plant (r = 0,78–0,81), the number of certified seeds and the number of seeds per plant (r = 0,84), the average number of beans per fertile node and the number of carpophores containing 2 beans (r = 0,74) that makes it possible to use them in assessing the productivity of plants.
Показать больше [+] Меньше [-]Characteristics of winter wheat varieties for resistance to causal agents and pests
2016
Ковалишина, Г. М | Муха, Т. І | Мурашко, Л. А | Заїма, О. А | Судденко, Ю. М
Purpose. Studying and identifying winter wheat varieties that are resistant to causal agents of major diseases and pests. Methods. Laboratory analysis, field study. Results. On artificial infection backgrounds of causal agents such varieties as ‘Smuhlianka’, ‘Svitanok Myronivskyi’, ‘Berehynia Myronіvska’, ‘Horlytsia Myronіvska’ have shown high level of resistance to brown rust; ‘Svitanok Myronivskyi’, ‘Berehynia Myronіvska’ – to powdery mildew; ‘Smuhlianka’ – to covered smut. Varieties ‘Voloshkova’, ‘Yuviliar Myronivskyi’, ‘Myrliena’, ‘Oberih Myronivskyi’, ‘Kolos Myronivschyny’, ‘Lehenda Myronivska’ had medium resistance to Septoria leaf blotch; ‘Smuhlianka’, ‘Myrliena’, ‘Oberih Myronivskyi’, ‘Berehynia Myronіvska’, ‘Horlytsia Myronіvska’, ‘Myronivska storichna’ – to Fusarium head blight; ‘Myronіvska 65’, ‘Smuhlianka’, ‘Lehenda Myronivska’, ‘Berehynia Myronіvska’ – to root rots. Among the varieties studied, there were those with group resistance to diseases: ‘Voloshkova’, ‘Myrliena’, ‘Yuviliar Myronivskyi’, ‘Oberih Myronivskyi’, ‘Bohdana’, ‘Myronivska storichna’, ‘Ekonomka’, ‘Svitanok Myronivskyi’, ‘Berehynia Myronіvska’, ‘Horlytsia Myronіvska’, ‘Smuhlianka’. Varieties bred at the V. M. Remeslo Myronivka Institute of Wheat are distinguished by pest resistance. During autumn tillering phase of winter wheat the smallest number of large cereal aphids was observed in varieties ‘Smuhlianka’ and ‘Myronivska storichna’, leafhoppers – in varieties ‘Smuhlianka’, ‘Myrliena’, ‘Yuviliar Myronivskyi’. The slight population of thrips in the phase of earing was marked in the variety ‘Kolos Myronivschyny’, in the milk-ripe stage the smallest number of larvae per ear was detected in varieties ‘Smuhlianka’, ‘Voloshkova’, ‘Kolos Myronivschyny’. Varieties ‘Lehenda Myronivska’ and ‘Smuhlianka’ were proved to be resistant to damages of stems by sawflies. It should be noted that ‘Smuhlianka’ variety shows resistance to aphids, leafhoppers and grain sawflies. Conclusions. It was established that winter wheat varieties bred at the V. M. Remeslo Myronivka Instutite of Wheat are characterized by resistance to certain causal agents, some of them – to their group. Varieties that are resistant to the most common and harmful phytophages were identified.
Показать больше [+] Меньше [-]Ukrainian cereals and oilseeds trade
2016
Духницький, Б. В | Сіліфонов, Т. В
Purpose. To analyze peculiarities of Ukrainian cereals and oilseeds trade, the situation on the world market, and determine future prospects of its development. Methods. Analysis and synthesis, comparative evaluation, graphic procedure. Results. The role and place of Ukraine in a total grain supply to the world market was determined. Ukraine is a world’s top ten grain producer. Among domestic agricultural products, cereals, oilseeds and sunflower oil are in the highest demand in the world. In recent years, our state has reinforced its status as one of the leading exporters of cereals. The commodity pattern of cereals and oilseeds export was analyzed with specifying most in-demand positions and the main countries purchasing these products. According to the results of 2015, Ukraine obtained the highest foreign currency revenue from export of corn, wheat and barley (in grain structure) including soybeans and rapeseed (among oil crops). Key domestic and multinational operators are the main exporters of cereals and oilseeds in Ukraine and still hold their leading position. It was found a significant excess of import price of seeds as compared with export price of crops grown in Ukraine. Assortment of maize and sunflower seeds offered by major companies-producers in Ukraine was studied. Main trends of the world grain market development are considered. Conclusions. It was established that Ukraine is one of the major exporters of cereals and oilseeds. However, volatility of their prices significantly affects the export revenue that was decreasing even with increasing export quantities in kind. The dependence of domestic grain industry development on high-quality imported seed of maize and sunflower hybrids was recorded. It is expected that in the years to come Ukraine will maintain its strong positions in the world’s grain market.
Показать больше [+] Меньше [-]Biological peculiarities of stem softwood cuttings of ornamental garden plants and their use in landscaping
2016
Мамчур, Т. В
Purpose. To study biological peculiarities of softwood cuttings of three species of ornamental garden plants of the Hydrangeaceae family introduced under the conditions of a botanical nursery of Uman National University of Horticulture, and the prospects of planting material use in landscaping. Methods. Field study, laboratory test, statistical and mathematical analysis. Results. Bioecological and ornamental peculiarities of grafters of investigated species and their preservation for supplying the store of initial plant graft material were studied. The dependence of regeneration ability of softwood cuttings of investigated varieties on preplant treatment by a biologically active substance (KANO), type of a shoot, time of grafting, etc was analyzed. Substantially intensive rooting of basal drafts with three nods and less intensive rooting of apical drafts was observed during the whole period of rooting. Conclusions. The treatment of softwood cuttings with a biologically active substance at the concentration of 10–15 mg/l in the aqueous solution stimulates root formation and activates growth of own root plants. Investigated plant species of Hydrangeaсeae family are promising for landscaping on urbanized areas of various functional use.
Показать больше [+] Меньше [-]Impact of cultivation technologies elements on winter wheat grain productivity and quality
2016
Олійник, К. М | Давидюк, Г. В | Блажевич, Л. Ю | Худолій, Л. В
Purpose. To develop and improve adaptive technologies of winter wheat cultivation which provide high productivity and quality of grain. Methods. Field and laboratory studies, mathematical and statistical analysis. Results. During 2011–2015, the impact of cultivation technologies on the formation of grain quality and yield of winter wheat variety ‘Stolychna’ (with pea as predecessor) was studied. When using alternative technologies with only predecessor by-products application, the yield of winter wheat with integrated crop protection system was 4,56 t/ha, with minimal protection – 4.25 t/ha with grain quality of the 5th class of B group. Resource saving cultivation technologies with limited application of fertilizers (Р45К45N30(II)+30(IV)) provided productivity at the level of 4,87–5,50 t/ha with grain quality of the 2nd–3rd class of A group. Grain yield of 6.01 t/ha with indicators of the 2nd–3rd class of quality was obtained with the use of intensive cultivation technology with application of mineral fertilizers (Р90К90N30(II)+60(IV)+30(VIII)) on the background of applying predecessor’s by-products and integrated crop protection. The highest yield of grain (6.22 t/ha) with indicators of the 2nd class of A group quality on average for the research period was provided by energy-intensive technology, that requires the application of mineral fertilizers (P135K135N60(II)+75(IV)+45(VIII)) and incorporation of predecessor’s by-products in the soil, and integrated plant protection. Conclusion. It was found that in the northern part of the Right-Bank Forest-Steppe zone of Ukraine, the highest productivity of winter wheat was obtained in dark grey podzolic soils when using the energy-intensive technology with application of P135K135N60(II)+75(IV)+45(VIII) on the background of predecessor’s by-products and integrated crop protection. This technology ensured the grain yield of 6.22 t/ha of the 2nd class of A group quality.
Показать больше [+] Меньше [-]On streamlining the Ukrainian names of plants. Information 6. Names of some subtribe Malinae Reveal taxa (information 6)
2016
Меженський, В. М
Purpose. To analyse the Ukrainian nomenclature of subtribe Malinae representatives and improve terminological system of the Ukrainian names of a species and a generic levels and names of fruit crops. Results. There is a confusion in the Ukrainian names of plants of Cydonia-Chaenomeles–Pseudocydonia, Mespilus–Eriobotrya, Amelanchier–Cotoneaster. Considering the division of Sorbus s.l. on separate genera Sorbus s.str., Aria, Cormus, Chamaemespilus, Torminalis and availability of numerous hybrids with their involvement, the Ukrainian names for both taxa and nothotaxa of species and generic levels are offered, principles of their choice are also considered. Formation of the Ukrainian generic names on the basis of specific epithets leads to undesirable tautonomy. Aspects of derussification of the Ukrainian professional terminology are discussed. Conclusions. Ukrainian scientific generic and nothogeneric plant names for subtribe Malinae are transliterated/transcripted, borrowed as a calque of latin names or have their own origin, consisting of one or two words. The choice of the best variants of generic and species names from a number of possible ones has a temporal component and largely depends on experts who apply them and basic principles of choice. It is necessary to improve the modern Ukrainian plant nomenclature on a national basis involving the transformed Latin scientific names for introduced species and hybrids.
Показать больше [+] Меньше [-]Applying analysis of variance to determine stability of morphological and value for cultivation and use characteristics of Lactuca sativa var. longifolia L. variety of lettuce ‘Skarb’
2016
Лещук, Н. В
Purpose. Providing a scientific rationale for the practical application of analysis of variance elements to determine the range of morphological and value for cultivation and use (VCU) characteristics of Romaine lettuce ‘Skarb’ variety. Results. The Romaine lettuce ‘Skarb’ variety was identified by the type of display of quantitative (QN) identification characteristics. VCU characteristics of the var. longifolia L. varieties were determined and statistically processed. The author focused on practical application of histogram and variation curve of phenotype morphological characteristics when performing the uniformity test. The correlation between quantitative characteristics of lettuce leaf and head was observed when plant productivity was developing. Romaine lettuce yield value was verified statistically. Thresholds were established for the indices under study, confidence intervals were defined over the years of study. Conclusions. The use of Lewis stability factor (S.F. = Xmax/Xmin) allowed to establish the stability of each characteristic display. Practical application of the histogram and variation curve for determining code of display of quantitative morphological characters and the range of VCU characteristics of the Romaine lettuce ‘Skarb’ variety was scientifically grounded.
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