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On streamlining the Ukrainian names of plants. Information 6. Names of some subtribe Malinae Reveal taxa (information 6) Полный текст
2016
Меженський, В. М
On streamlining the Ukrainian names of plants. Information 6. Names of some subtribe Malinae Reveal taxa (information 6) Полный текст
2016
Меженський, В. М
Purpose. To analyse the Ukrainian nomenclature of subtribe Malinae representatives and improve terminological system of the Ukrainian names of a species and a generic levels and names of fruit crops. Results. There is a confusion in the Ukrainian names of plants of Cydonia-Chaenomeles–Pseudocydonia, Mespilus–Eriobotrya, Amelanchier–Cotoneaster. Considering the division of Sorbus s.l. on separate genera Sorbus s.str., Aria, Cormus, Chamaemespilus, Torminalis and availability of numerous hybrids with their involvement, the Ukrainian names for both taxa and nothotaxa of species and generic levels are offered, principles of their choice are also considered. Formation of the Ukrainian generic names on the basis of specific epithets leads to undesirable tautonomy. Aspects of derussification of the Ukrainian professional terminology are discussed. Conclusions. Ukrainian scientific generic and nothogeneric plant names for subtribe Malinae are transliterated/transcripted, borrowed as a calque of latin names or have their own origin, consisting of one or two words. The choice of the best variants of generic and species names from a number of possible ones has a temporal component and largely depends on experts who apply them and basic principles of choice. It is necessary to improve the modern Ukrainian plant nomenclature on a national basis involving the transformed Latin scientific names for introduced species and hybrids.
Показать больше [+] Меньше [-]On streamlining the Ukrainian names of plants. Information 6. Names of some subtribe Malinae Reveal taxa (information 6) Полный текст
2016
В. М. Меженський
Purpose. To analyse the Ukrainian nomenclature of subtribe Malinae representatives and improve terminological system of the Ukrainian names of a species and a generic levels and names of fruit crops. Results. There is a confusion in the Ukrainian names of plants of Cydonia-Chaenomeles–Pseudocydonia, Mespilus–Eriobotrya, Amelanchier–Cotoneaster. Considering the division of Sorbus s.l. on separate genera Sorbus s.str., Aria, Cormus, Chamaemespilus, Torminalis and availability of numerous hybrids with their involvement, the Ukrainian names for both taxa and nothotaxa of species and generic levels are offered, principles of their choice are also considered. Formation of the Ukrainian generic names on the basis of specific epithets leads to undesirable tautonomy. Aspects of derussification of the Ukrainian professional terminology are discussed. Conclusions. Ukrainian scientific generic and nothogeneric plant names for subtribe Malinae are transliterated/transcripted, borrowed as a calque of latin names or have their own origin, consisting of one or two words. The choice of the best variants of generic and species names from a number of possible ones has a temporal component and largely depends on experts who apply them and basic principles of choice. It is necessary to improve the modern Ukrainian plant nomenclature on a national basis involving the transformed Latin scientific names for introduced species and hybrids.
Показать больше [+] Меньше [-]Impact of cultivation technologies elements on winter wheat grain productivity and quality Полный текст
2016
Олійник, К. М | Давидюк, Г. В | Блажевич, Л. Ю | Худолій, Л. В
Impact of cultivation technologies elements on winter wheat grain productivity and quality Полный текст
2016
Олійник, К. М | Давидюк, Г. В | Блажевич, Л. Ю | Худолій, Л. В
Purpose. To develop and improve adaptive technologies of winter wheat cultivation which provide high productivity and quality of grain. Methods. Field and laboratory studies, mathematical and statistical analysis. Results. During 2011–2015, the impact of cultivation technologies on the formation of grain quality and yield of winter wheat variety ‘Stolychna’ (with pea as predecessor) was studied. When using alternative technologies with only predecessor by-products application, the yield of winter wheat with integrated crop protection system was 4,56 t/ha, with minimal protection – 4.25 t/ha with grain quality of the 5th class of B group. Resource saving cultivation technologies with limited application of fertilizers (Р45К45N30(II)+30(IV)) provided productivity at the level of 4,87–5,50 t/ha with grain quality of the 2nd–3rd class of A group. Grain yield of 6.01 t/ha with indicators of the 2nd–3rd class of quality was obtained with the use of intensive cultivation technology with application of mineral fertilizers (Р90К90N30(II)+60(IV)+30(VIII)) on the background of applying predecessor’s by-products and integrated crop protection. The highest yield of grain (6.22 t/ha) with indicators of the 2nd class of A group quality on average for the research period was provided by energy-intensive technology, that requires the application of mineral fertilizers (P135K135N60(II)+75(IV)+45(VIII)) and incorporation of predecessor’s by-products in the soil, and integrated plant protection. Conclusion. It was found that in the northern part of the Right-Bank Forest-Steppe zone of Ukraine, the highest productivity of winter wheat was obtained in dark grey podzolic soils when using the energy-intensive technology with application of P135K135N60(II)+75(IV)+45(VIII) on the background of predecessor’s by-products and integrated crop protection. This technology ensured the grain yield of 6.22 t/ha of the 2nd class of A group quality.
Показать больше [+] Меньше [-]Impact of cultivation technologies elements on winter wheat grain productivity and quality Полный текст
2016
К. М. Олійник | Г. В. Давидюк | Л. Ю. Блажевич | Л. В. Худолій
Purpose. To develop and improve adaptive technologies of winter wheat cultivation which provide high productivity and quality of grain. Methods. Field and laboratory studies, mathematical and statistical analysis. Results. During 2011–2015, the impact of cultivation technologies on the formation of grain quality and yield of winter wheat variety ‘Stolychna’ (with pea as predecessor) was studied. When using alternative technologies with only predecessor by-products application, the yield of winter wheat with integrated crop protection system was 4,56 t/ha, with minimal protection – 4.25 t/ha with grain quality of the 5th class of B group. Resource saving cultivation technologies with limited application of fertilizers (Р45К45N30(II)+30(IV)) provided productivity at the level of 4,87–5,50 t/ha with grain quality of the 2nd–3rd class of A group. Grain yield of 6.01 t/ha with indicators of the 2nd–3rd class of quality was obtained with the use of intensive cultivation technology with application of mineral fertilizers (Р90К90N30(II)+60(IV)+30(VIII)) on the background of applying predecessor’s by-products and integrated crop protection. The highest yield of grain (6.22 t/ha) with indicators of the 2nd class of A group quality on average for the research period was provided by energy-intensive technology, that requires the application of mineral fertilizers (P135K135N60(II)+75(IV)+45(VIII)) and incorporation of predecessor’s by-products in the soil, and integrated plant protection. Conclusion. It was found that in the northern part of the Right-Bank Forest-Steppe zone of Ukraine, the highest productivity of winter wheat was obtained in dark grey podzolic soils when using the energy-intensive technology with application of P135K135N60(II)+75(IV)+45(VIII) on the background of predecessor’s by-products and integrated crop protection. This technology ensured the grain yield of 6.22 t/ha of the 2nd class of A group quality.
Показать больше [+] Меньше [-]Assessment of stability and plasticity of new hybrids of maize (Zea mays L.) under the conditions of Polissia and Steppe zones of Ukraine Полный текст
2016
Присяжнюк, Л. М | Шовгун, О. О | Король, Л. В | Коровко, І. І
Assessment of stability and plasticity of new hybrids of maize (Zea mays L.) under the conditions of Polissia and Steppe zones of Ukraine Полный текст
2016
Присяжнюк, Л. М | Шовгун, О. О | Король, Л. В | Коровко, І. І
Purpose. To select promising high productive maize hybrids of middle-early maturity group in terms of stability and plasticity of main economic characters. Methods. Field study, laboratory test, analytical procedure and statistical evaluation. Results. 14 maize hybrids recorded in the State Register of Plant Varieties Suitable for Dissemination in Ukraine in 2015 were studied for plasticity and stability of such traits as productivity, protein and starch content. Intensive highly-plastic hybrid ‘SI Tiptop’ was selected among the studied ones for productivity trait that can respond properly to changes of growing conditions. It was defined that for the starch content such hybrids as ‘SI Tiptop’, ‘SI Enigma’, ‘SI Arioso’, ‘Svich 38’, ‘Svich 35’, ‘HU 8653’, ‘Zdobutok’ and ‘SI Contrakt’ belonged to the intensive type and combined rather high values and the stability of the studied trait under variable conditions. The following hybrids as ‘NS 2642’, ‘DK S3016’, ‘Svich 38’, ‘NS 2632’ were qualified as intensive for protein content and appeared to be highly-plastic but stability values of this trait were low. ‘Svich 38’ hybrid was intensive simultaneously for two traits such as protein and starch content and showed rather high values of plasticity. ‘SI Tiptop’, ‘SI Enigma’ and ‘Svich 35’ were defined as hybrids of extensive type that provided stable protein content in adverse cultivation conditions. Conclusions. On the condition that intensive crop growing technologies should be used, for obtaining stable yields it is advisable to sow only highly-plastic hybrids that can adapt to unfavorable environmental factors, including ‘SI Tiptop’ – for productivity trait, ‘Zdobutok’ and ‘SI Kontrakt’ – for starch content, ‘MAC 24N‘, ‘NA 2642‘ and ‘Danubio’ – for protein content.
Показать больше [+] Меньше [-]Assessment of stability and plasticity of new hybrids of maize (Zea mays L.) under the conditions of Polissia and Steppe zones of Ukraine Полный текст
2016
Л. М. Присяжнюк | О. О. Шовгун | Л. В. Король | І. І. Коровко
Purpose. To select promising high productive maize hybrids of middle-early maturity group in terms of stability and plasticity of main economic characters. Methods. Field study, laboratory test, analytical procedure and statistical evaluation. Results. 14 maize hybrids recorded in the State Register of Plant Varieties Suitable for Dissemination in Ukraine in 2015 were studied for plasticity and stability of such traits as productivity, protein and starch content. Intensive highly-plastic hybrid ‘SI Tiptop’ was selected among the studied ones for productivity trait that can respond properly to changes of growing conditions. It was defined that for the starch content such hybrids as ‘SI Tiptop’, ‘SI Enigma’, ‘SI Arioso’, ‘Svich 38’, ‘Svich 35’, ‘HU 8653’, ‘Zdobutok’ and ‘SI Contrakt’ belonged to the intensive type and combined rather high values and the stability of the studied trait under variable conditions. The following hybrids as ‘NS 2642’, ‘DK S3016’, ‘Svich 38’, ‘NS 2632’ were qualified as intensive for protein content and appeared to be highly-plastic but stability values of this trait were low. ‘Svich 38’ hybrid was intensive simultaneously for two traits such as protein and starch content and showed rather high values of plasticity. ‘SI Tiptop’, ‘SI Enigma’ and ‘Svich 35’ were defined as hybrids of extensive type that provided stable protein content in adverse cultivation conditions. Conclusions. On the condition that intensive crop growing technologies should be used, for obtaining stable yields it is advisable to sow only highly-plastic hybrids that can adapt to unfavorable environmental factors, including ‘SI Tiptop’ – for productivity trait, ‘Zdobutok’ and ‘SI Kontrakt’ – for starch content, ‘MAC 24N‘, ‘NA 2642‘ and ‘Danubio’ – for protein content.
Показать больше [+] Меньше [-]Evaluation of pea varieties based on correlation of quantitative traits and indices Полный текст
2016
Присяжнюк, О. І | Король, Л. В
Evaluation of pea varieties based on correlation of quantitative traits and indices Полный текст
2016
Присяжнюк, О. І | Король, Л. В
Purpose. To evaluate pea plants productivity and determine the degree of correlation among the main agronomic characters in pea varieties. Methods. Structural and statistical analysis. Results. Evaluation of pea samples in terms of indices of plant productivity elements level based on correlation analysis and single-factor indices appliance provided insight into the ratio of one trait share per unit of another one. It was defined that some correlations among the elements of productivity was not only moderate and weak, but they also changed their sign that could be the evidence of growth conditions influence on structural relationships between some traits and, consequently, redistribution of their contributions to the formation of variety productivity. Positive and very close relationship of many traits was revealed, particularly between plant height and the height of the plant up to the first bean, the number of nodes and the number of sterile nodes (r = 0,95–0,97). Methodological aspects of the variety model creation were considered, that may be useful not only in pea breeding but also for improving the technology of its cultivation. Conclusions. Correlation relationships were established between the number of beans and the number of fruiting nodes and the number of carpophores containing 2 beans (r = 0,86–0,88), seed mass and plant mass (r = 0,81), the number of seeds per plant and plant mass and seed mass per plant (r = 0,78–0,81), the number of certified seeds and the number of seeds per plant (r = 0,84), the average number of beans per fertile node and the number of carpophores containing 2 beans (r = 0,74) that makes it possible to use them in assessing the productivity of plants.
Показать больше [+] Меньше [-]Особливості формування ринку сої культурної в Україні Полный текст
2018
С. В. Васьківська | Н. С. Орленко | С. О. Ткачик | Л. В. Худолій
Мета. Проаналізувати тенденції виробництва сої культурної в світі та виявити їх вплив на ринок сортів сої культурної в Україні. Виявити динаміку подачі та реєстрації заявок на включення до Державного реєстру сортів рослин, придатних для поширення в Україні. Порівняти урожайність сої культурної у лідерів глобального ринку сої з урожайністю сої культурної, що знаходиться у виробництві в Україні. Висвітлити інформацію щодо кращих за урожайністю нових сортів сої культурної, які включені до Державного реєстру сортів рослин, придатних для поширення в Україні. Методи. Аналітичний, математичний, статистичний, графічний. Результати. Виявлено помітний вплив тенденцій на глобальному ринку сої на динаміку виробництва цієї культури в Україні та скорочення у десятки разів обсягів відставання виробництва сої культурної в Україні порівняно із світовими лідерами в десятки разів. Тенденції приросту виробництва співпадають з тенденціями лідерів глобального ринку сої США, Бразилії та Аргентини. Визначено вплив зростання виробництва на процес реєстрації нових сортів сої культурної у Реєстрі сортів рослин України. З’ясовано, що тренд зростання кількості поданих заявок носить поліноміальний характер. Встановлено, що найбільша кількість сортів сої культурної, що проходила кваліфікаційну експертизу мала українське походження. Вивчено сорти іноземного походження з Канади, Франції, Австрії, США, Німеччини, Аргентини, Кіпру, Польщі, Республіки Сербія, Румунії, Республіки Хорватії, Чеської Республіки, Швейцарії. Визначено нові сорти сої культурної, які мали найвищу врожайність за період кваліфікаційної експертизи з 2010 по 2018 рік. Висновки. Темпи приросту виробництва сої культурної в Україні мають позитивну динаміку. В Реєстрі сортів рослин України переважають сорти українського походження. Середній рівень урожайності сортів сої культурної за 2010–2018 роки відповідає середній врожайності цієї культури у світі. Максимальну врожайність серед сортів, які проходили кваліфікаційну експертизу сортів рослин у період з 2010 по 2018 рік, проявили сорти: ‘Silesia’ французького походження, ‘Естафета’, ‘Терек’, ‘Авантюрин’ українського та сорт канадського походження ‘Kofu’.
Показать больше [+] Меньше [-]Physiological role of amino acids in the nutrition of highly productive varieties of winter wheat Полный текст
2016
Швартау, В. В | Михальська, Л. М | Мірошниченко, І. М
Physiological role of amino acids in the nutrition of highly productive varieties of winter wheat Полный текст
2016
Швартау, В. В | Михальська, Л. М | Мірошниченко, І. М
Purpose. To define the role of fertilizers containing amino acids in the nutrition systems of highly productive varieties of winter wheat. Methods. Field studies, biochemical technique, analytical procedure, statistical evaluation. Results. In the process of investigations, sufficiently high activity of fertilizers containing amino acids was established when applying them for leaf-feeding. Fertilizers based on algae (Megafol, Megafol Protein, Terra-Sorb foliar) and animal (Izabion) hydrolysates appeared to be very effective for increasing yield of culture. It was found that in case of low doses of mineral nitrogen, fertilizers helped to increase both the yield and quality indicators of high-yielding wheat variety. Conclusions. It was defined that fertilizers that include amino acids are highly effective compositions containing plant-available nitrogen in organic form and can be promising for application as antistress agents and improving efficiency of macro- and microelements use by cultivated plants. They are essential components of modern technologies for cultivation of highly productive varieties and hybrids in crop production of Ukraine.
Показать больше [+] Меньше [-]Physiological role of amino acids in the nutrition of highly productive varieties of winter wheat Полный текст
2016
В. В. Швартау | Л. М. Михальська | І. М. Мірошниченко
Purpose. To define the role of fertilizers containing amino acids in the nutrition systems of highly productive varieties of winter wheat. Methods. Field studies, biochemical technique, analytical procedure, statistical evaluation. Results. In the process of investigations, sufficiently high activity of fertilizers containing amino acids was established when applying them for leaf-feeding. Fertilizers based on algae (Megafol, Megafol Protein, Terra-Sorb foliar) and animal (Izabion) hydrolysates appeared to be very effective for increasing yield of culture. It was found that in case of low doses of mineral nitrogen, fertilizers helped to increase both the yield and quality indicators of high-yielding wheat variety. Conclusions. It was defined that fertilizers that include amino acids are highly effective compositions containing plant-available nitrogen in organic form and can be promising for application as antistress agents and improving efficiency of macro- and microelements use by cultivated plants. They are essential components of modern technologies for cultivation of highly productive varieties and hybrids in crop production of Ukraine.
Показать больше [+] Меньше [-]Plant DNA banks for genetic resources conservation (review) Полный текст
2016
Волкова, Н. Е
Plant DNA banks for genetic resources conservation (review) Полный текст
2016
Волкова, Н. Е
Purpose. Literature review of DNA banks creation as the current strategy of plant genetic resources conservation. Results. The current state of plant genetic resources conservation was analyzed in the context of the threat of genetic erosion. The importance of DNA banks was shown which function is to store DNA samples and associated products and disseminate them for research purposes. The main DNA banks in the world were described, including the Republican DNA Bank of Human, Animals, Plants and Microorganisms at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus. Stages of DNA banking were considered: tissue sampling (usually from leaves), cell destruction, DNA extraction, DNA storage. Different methods of tissue sampling, extraction and DNA storage were compared. The need for Plant DNA Bank creation in Ukraine was highlighted. Conclusions. DNA collections is an important resource in the global effort to overcome the crisis in biodiversity, for managing world genetic resources and maximizing their potential.
Показать больше [+] Меньше [-]Plant DNA banks for genetic resources conservation (review) Полный текст
2016
Н. Е. Волкова
Purpose. Literature review of DNA banks creation as the current strategy of plant genetic resources conservation. Results. The current state of plant genetic resources conservation was analyzed in the context of the threat of genetic erosion. The importance of DNA banks was shown which function is to store DNA samples and associated products and disseminate them for research purposes. The main DNA banks in the world were described, including the Republican DNA Bank of Human, Animals, Plants and Microorganisms at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus. Stages of DNA banking were considered: tissue sampling (usually from leaves), cell destruction, DNA extraction, DNA storage. Different methods of tissue sampling, extraction and DNA storage were compared. The need for Plant DNA Bank creation in Ukraine was highlighted. Conclusions. DNA collections is an important resource in the global effort to overcome the crisis in biodiversity, for managing world genetic resources and maximizing their potential.
Показать больше [+] Меньше [-]Method for determination of varietal purity (typicality), hybridity, sterility of seed lots based on the establishment of the quantitative ratio of alleles of DNA markers Полный текст
2016
Вдовиченко, Ж. В | Спиридонов, В. Г | Хомутовська, С. В | Парій, М. Ф
Method for determination of varietal purity (typicality), hybridity, sterility of seed lots based on the establishment of the quantitative ratio of alleles of DNA markers Полный текст
2016
Вдовиченко, Ж. В | Спиридонов, В. Г | Хомутовська, С. В | Парій, М. Ф
Purpose. To develop a conceptually new method for determination of varietal purity (typicality), hybridity, sterility of seed lots. Methods of molecular biology (genomic DNA extraction, PCR with SSR markers application, capillary electrophoresis), genetic, statistical, mathematical analysis. Results. New method for determining the varietal qualities of seed lot was developed that consists of the following steps: simultaneous DNA extraction from a representative sample of aggregated seeds; PCR and further analysis of the amplification products by determination of the qualitative and quantitative composition of SSR-markers’ alleles; calculation of values of varietal seed lot quality using experimentally derived allele ratios. Conclusions. The developed method for determining varietal qualities of seed lots allows to reduce significantly the consumption of materials, time and labor during the analysis. Consistent qualification and quantification of alleles in the total sample of a seed lot is a conceptually new approach to establish varietal purity (typicality), hybridity, sterility.
Показать больше [+] Меньше [-]A revision of Salispina, its placement in a new family, Salispinaceae (Rhipidiales), and description of a fourth species, S. hoi sp. nov Полный текст
2018
Reuel M. Bennett | Mark Kevin Devanadera | Gina R. Dedeles | Marco Thines
Abstract The genus Salispina was recently described for saprotrophic estuarine oomycetes with aculeolate or spiny sporangia. The genus currently contains three species, S. intermedia, S. lobata, and S. spinosa, the latter two previously included in Halophytophthora. During a survey of mangrove-inhabiting oomycetes in the Philippines, an isolate of Salispina (USTCMS 1611), was obtained from a decaying mangrove leaf. This isolate differed from other species in the genus in a unique combination of morphological and biological characters. Phylogenetic analysis revealed it to be the sister lineage of S. lobata. Consequently, the new species name S. hoi is introduced for the isolate. In addition, Salispina spp. grouped with Sapromyces of Rhipidiales with strong support, but differs from all other known genera of the order in the weak formation of hyphal constrictions, and absence of basal thalli and a holdfast network. The new family Salispinaceae is, therefore, described to accommodate Salispina in the order Rhipidiales.
Показать больше [+] Меньше [-]Functional features of photosynthetic apparatus of modern high-yielding winter wheat varieties Полный текст
2016
Моргун, В. В | Стасик, О. О | Кірізій, Д. А | Прядкіна, Г. О
Functional features of photosynthetic apparatus of modern high-yielding winter wheat varieties Полный текст
2016
Моргун, В. В | Стасик, О. О | Кірізій, Д. А | Прядкіна, Г. О
Purpose. Comparative studies of the photosynthetic apparatus of winter wheat varieties of modern and earlier breeding in the relation to their productivity. Methods. Field, pot experiment, biometrical, gas analysis, analytical and statistical ones. Results. It is found that the modern high-yielding winter wheat varieties have a higher rate of flag leaf photosynthesis during generative period of development, particularly at grain filling period, improved ability to store assimilates in stem and effectively use them later for grain growth. The modern varieties form crop canopy with greater leaf area and chlorophyll indexes and larger photosynthetic capacity and maintain functional activity of photosynthetic apparatus longer at the end of the growing season. Conclusions. The superiority for grain productivity of modern high-yielding wheat varieties, originated from the Institute of Plant Physiology and Genetics, NAS of Ukraine, over varieties released before the “green revolution”, results from increased capacity and durability of functioning the crop photosynthetic apparatus, higher CO2 assimilation rate and optimization of source-sink relations in whole plant.
Показать больше [+] Меньше [-]Peculiarities of grain technological indices establishment for coated and naked grain oats (Avena sativa L.) Полный текст
2013
О. І. Буняк
Oats technological indices have been analyzed. Influence of growing conditions on natural weight, kernel size, and hoodness is established. The study summary indicates essential dependence of natural weight and hoodness and precipitation at the stage of grain formation in coated grain oats varieties. Moisture supply in the period of grain formation essentially influenced the percentage of coated grains formation and natural weight score in naked grain varieties. Morphometric analyzes of oats caryopsis was completed using the method of machine vision. Correlation between technological indices and morphometric characteristics of naked and coated grain of oats was determined. Also, essential dependence of hoodness on 1000 seeds weight and the lack of essential correlation with morphometric characteristics of the calliopsis were established. Calliopsis width essentially influenced the weight of 1000 seeds. Metric indices of naked grain oats calliopsis exposed close correlation with kernel size and natural weight. Weight of 1000 seeds exposed essential varietal distinctness and was determined by genetic peculiarities of varieties
Показать больше [+] Меньше [-]Bioinformatic analysis of maize gene encoding starch branching enzyme SBEIIb Полный текст
2016
Сліщук, Г. І | Жернаков, Т. Ю | Волкова, Н. Е
Bioinformatic analysis of maize gene encoding starch branching enzyme SBEIIb Полный текст
2016
Сліщук, Г. І | Жернаков, Т. Ю | Волкова, Н. Е
Purpose. Investigation of maize ae1 gene polymorphism by bioinformatic methods. Methods. Global and local alignment of the nucleotide and amino acid sequences, in silico translation and transcription, translates modeling, primers design, phylogenetic analysis. Results. 255 nucleotide sequences of maize аe1 gene, 500 amino acid sequences of homology translates of maize ae1 gene (SBEIIb enzyme homologs) and 100 mRNA expressed from the maize ae1 gene were analyzed to establish phylogenetic relationships. Polymorphism of maize ae1 gene different regions was investigated by bioinformatic methods. Modeling of the maize enzyme SBEIIb was performed. Conclusions. According to the results of amino acid sequences of SBEIIb enzyme homologs alignment, it was found that ae1 gene orthologs are present only in monocots, paralogs – in monocots, dicots, and other taxa, including algae and animals. Based on the results of alignment of plants mRNA from which enzyme SBEIIb is translated, maize ae1 gene orthologs and the nearest paralogs encoding starch branching enzymes with chloroplast localization were defined; this suggests a possible origin of ae1 gene due to duplication of the gene encoding the 1,4-alpha-glucan-branching enzyme 2 with chloroplast or amyloplast localization. In the maize ae1 gene structure, regions were found that include polymorphic sites not defined previously. For the polymorphic sites design primers were developed that allowed to differentiate the maize lines. It was determined that the detection of polymorphism in theory can influence the enzyme function and, as a result, change the concentration of amylopectin in maize grain.
Показать больше [+] Меньше [-]Bioinformatic analysis of maize gene encoding starch branching enzyme SBEIIb. Полный текст
2016
Г. І. Сліщук | Т. Ю. Жернаков | Н. Е. Волкова
Purpose. Investigation of maize ae1 gene polymorphism by bioinformatic methods. Methods. Global and local alignment of the nucleotide and amino acid sequences, in silico translation and transcription, translates modeling, primers design, phylogenetic analysis. Results. 255 nucleotide sequences of maize аe1 gene, 500 amino acid sequences of homology translates of maize ae1 gene (SBEIIb enzyme homologs) and 100 mRNA expressed from the maize ae1 gene were analyzed to establish phylogenetic relationships. Polymorphism of maize ae1 gene different regions was investigated by bioinformatic methods. Modeling of the maize enzyme SBEIIb was performed. Conclusions. According to the results of amino acid sequences of SBEIIb enzyme homologs alignment, it was found that ae1 gene orthologs are present only in monocots, paralogs – in monocots, dicots, and other taxa, including algae and animals. Based on the results of alignment of plants mRNA from which enzyme SBEIIb is translated, maize ae1 gene orthologs and the nearest paralogs encoding starch branching enzymes with chloroplast localization were defined; this suggests a possible origin of ae1 gene due to duplication of the gene encoding the 1,4-alpha-glucan-branching enzyme 2 with chloroplast or amyloplast localization. In the maize ae1 gene structure, regions were found that include polymorphic sites not defined previously. For the polymorphic sites design primers were developed that allowed to differentiate the maize lines. It was determined that the detection of polymorphism in theory can influence the enzyme function and, as a result, change the concentration of amylopectin in maize grain.
Показать больше [+] Меньше [-]Bioecological peculiarities of mid-ripening varieties of strawberry (Fragaria ananassa Duch.) in the northern part of the Forest-Steppe zone of Ukraine Полный текст
2016
Павлюк, В. В | Ковальчук, Н. С | Павлюк, Н. В
Bioecological peculiarities of mid-ripening varieties of strawberry (Fragaria ananassa Duch.) in the northern part of the Forest-Steppe zone of Ukraine Полный текст
2016
Павлюк, В. В | Ковальчук, Н. С | Павлюк, Н. В
Purpose. Improvement of a range of strawberry varieties based on comprehensive study and introduction of new Ukrainian and West European cultivars. Methods. Cultivars and elite forms were studied using the methods of primary variety studying and state variety testing at three-year strawberry plantations of the Institute of Horticulture of the National Academy of Sciences of Ukraine (the city of Kyiv) and its variety testing stations. Results. It was found that more frequent spring and summer droughts as well as black frosts in winter adversely affected the strawberry productivity. The best varieties were selected among new ones for the complex of traits, they included ‘Elegance’ from the Great Britain, ‘Gercules’ from Ukraine (both are dessert grapes), ‘Elsanta’ from Holland (for universal use), ‘Alba’ from Italia (as raw material). The highly adaptive and productive ‘Istochnik’ and ‘Festyvalna romashka’ varieties (as raw material) are recommended for the north regions. Conclusions. In the context of profound climatic change, high-profitable cultivation of mid-ripening varieties and a strawberry as such in the Northern Forest-Steppe zone is expedient under controlled conditions – in high film tunnels, in hot-houses and under perennial irrigation that already has become the European tendency.
Показать больше [+] Меньше [-]Bioecological peculiarities of mid-ripening varieties of strawberry (Fragaria ananassa Duch.) in the northern part of the Forest-Steppe zone of Ukraine Полный текст
2016
В. В. Павлюк | Н. С. Ковальчук | Н. В. Павлюк
Purpose. Improvement of a range of strawberry varieties based on comprehensive study and introduction of new Ukrainian and West European cultivars. Methods. Cultivars and elite forms were studied using the methods of primary variety studying and state variety testing at three-year strawberry plantations of the Institute of Horticulture of the National Academy of Sciences of Ukraine (the city of Kyiv) and its variety testing stations. Results. It was found that more frequent spring and summer droughts as well as black frosts in winter adversely affected the strawberry productivity. The best varieties were selected among new ones for the complex of traits, they included ‘Elegance’ from the Great Britain, ‘Gercules’ from Ukraine (both are dessert grapes), ‘Elsanta’ from Holland (for universal use), ‘Alba’ from Italia (as raw material). The highly adaptive and productive ‘Istochnik’ and ‘Festyvalna romashka’ varieties (as raw material) are recommended for the north regions. Conclusions. In the context of profound climatic change, high-profitable cultivation of mid-ripening varieties and a strawberry as such in the Northern Forest-Steppe zone is expedient under controlled conditions – in high film tunnels, in hot-houses and under perennial irrigation that already has become the European tendency.
Показать больше [+] Меньше [-]