Purpose. To define statistical methods and tools (application packages) for creating the decision support system (DSS) for qualifying examination of plant varieties suitable for dissemination (VSD) in the context of data processing tasks. To substantiate the selection of software for proces­sing statistical data relative to field and laboratory investigations that are included into the qualifying examination for VSD. Methods. Analytical one based on the comparison of methods of descriptive and multivariate statistics and tools of intellectual analysis of data obtained during qualifying examination for VSD. Comparative analysis of software tools for processing statistical data in order to prepare proposals for the final decision on plant variety application. Decomposition of tasks was carried out which were included into the decision support system for qualifying examination of varieties-candidates for VSD. Results. Statistical package SPSS, analysis package included in MS Excel and programe language R was compared for the following criteria: interface usability, functionality, quality of calculation result presentation, visibility of graphical information, software cost. The both packages were widely used in the world for statistical data processing, they have similar functions for statistics calculation. Conclusion. Tasks of VSD were separated and recommended to tackle using investigated tools. Programe language R was a product recommended to use as a tool. The main advantage of R as compared to the package IBM SPSS Statistics is the fact that R is an open source software.

Мета.Визначити статистичні методи та інструментальні засоби (пакети прикладних програм) для створення сис­теми підтримки прийняття рішення (СППР) кваліфікаційної експертизи сортів рослин на придатність до поширення (ПСП) у розрізі завдань оброблення даних. Обґрунтувати вибір програмних засобів оброблення статистичних даних польових та лабораторних досліджень, що входять до складу кваліфікаційної експертизи на ПСП. Методи. Аналітичний, який ґрунтується на порівнянні методів описової та багатовимірної статистики й засобів інтелектуального аналізу даних, отриманих під час кваліфікаційної експертизи на ПСП. Порівняльний аналіз програмних засобів оброблення статистичних даних для підготовки пропозицій щодо кінцевого рішення за заявкою на сорт рослин.Результати. Проведено декомпозицію завдань, що входять до складу системи підтримки прийняття рішень з кваліфікаційної екс­пертизи сортів-кандидатів на ПСП. Проведено порівняння статистичного пакета SPSS, пакета аналізу, що входить до складу MS Excel, та мови програмування R за критеріями: зручність інтерфейсу, функціональність, якість представлення результатів обчислення, наочність графічної інформації, вартість програмного засобу. Обидва пакети широко застосовують у світі для статистичного оброблення даних, вони мають аналогічний склад функцій для обчислення статистик. Висновки. Виокремлено завдання ПСП, що рекомендовано вирішувати із застосуванням досліджуваних засобів. Як інструментальний засіб рекомендовано використовувати мову програмування R. Основною перевагою R порівняно з пакетом IBM SPSS Statistics є те, що R є програмним продуктом з відкритим кодом, який вільно поширюється. | Цель. Определить статистические методы и инструментальные средства (пакеты прикладных программ) для создания системы поддержки принятия решения (СППР) квалификационной экспертизы сортов на пригодность их к распространению (ПСР) в разрезе задач обработки данных. Обосновать выбор программных средств обработки статистических данных полевых и лабораторных исследований, которые входят в состав квалификационной экспертизы на ПСР. Методы. Аналитический, основанный на сравнении методов описательной и многомерной статистики и средств интеллектуального анализа данных, полученных при проведении квалификационной экспертизы на ПСР. Сравнительный анализ программных средств обработки статистических данных для подготовки предложений по окончательному решению по заявке на сорт растений.Результаты. Проведена декомпозиция задач, которые входят в состав системы поддержки принятия решений по квалификационной экспертизе сортов-кандидатов на ПСП. Проведено сравнение статистического пакета SPSS, пакета анализа, который входит в состав MS Excel и языка программирования R по критериям: удобство интерфейса, функциональность, качество представления результатов расчетов, наглядность графической информации, стоимость программного средства. Оба пакета широко применяются в мире для статистической обработки данных, имеют аналогичный состав функций для расчета статистик. Выводы. Выделены задачи ПСР, которые рекомендуется решать с применением исследуемых средств. В качестве инструментального средства целесообратно использовать язык программирования R. Основным преимуществом R по сравнению с пакетом IBM SPSS Statistics является то, что R – программный продукт с открытым кодом, который свободно распространяется. | Purpose. To define statistical methods and tools (application packages) for creating the decision support system (DSS) for qualifying examination of plant varieties suitable for dissemination (VSD) in the context of data processing tasks. To substantiate the selection of software for proces­sing statistical data relative to field and laboratory investigations that are included into the qualifying examination for VSD.Methods. Analytical one based on the comparison of methods of descriptive and multivariate statistics and tools of intellectual analysis of data obtained during qualifying examination for VSD. Comparative analysis of software tools for processing statistical data in order to prepare proposals for the final decision on plant variety application. Decomposition of tasks was carried out which were included into the decision support system for qualifying examination of varieties-candidates for VSD. Results. Statistical package SPSS, analysis package included in MS Excel and programe language R was compared for the following criteria: interface usability, functionality, quality of calculation result presentation, visibility of graphical information, software cost. The both packages were widely used in the world for statistical data processing, they have similar functions for statistics calculation. Conclusion. Tasks of VSD were separated and recommended to tackle using investigated tools. Programe language R was a product recommended to use as a tool. The main advantage of R as compared to the package IBM SPSS Statistics is the fact that R is an open source software.

Purpose. To define statistical methods and tools (application packages) for creating the decision support system (DSS) for qualifying examination of plant varieties suitable for dissemination (VSD) in the context of data processing tasks. To substantiate the selection of software for proces­sing statistical data relative to field and laboratory investigations that are included into the qualifying examination for VSD. Methods. Analytical one based on the comparison of methods of descriptive and multivariate statistics and tools of intellectual analysis of data obtained during qualifying examination for VSD. Comparative analysis of software tools for processing statistical data in order to prepare proposals for the final decision on plant variety application. Decomposition of tasks was carried out which were included into the decision support system for qualifying examination of varieties-candidates for VSD. Results. Statistical package SPSS, analysis package included in MS Excel and programe language R was compared for the following criteria: interface usability, functionality, quality of calculation result presentation, visibility of graphical information, software cost. The both packages were widely used in the world for statistical data processing, they have similar functions for statistics calculation. Conclusion. Tasks of VSD were separated and recommended to tackle using investigated tools. Programe language R was a product recommended to use as a tool. The main advantage of R as compared to the package IBM SPSS Statistics is the fact that R is an open source software.

Purpose. To obtain Miscanthus sacchariflorus (Maxim.) Hack and Miscanthus sinensis Andersson in vitro culture by indirect morphogenesis. Methods. Biotechnological procedures, mathematical and statistical analyses. Results. Composition of nutrient medium was developed intended for induction of callusogenesis from Miscanthus seeds with a poor germination and viability of seedlings – Murashige and Skoog (MS) medium was modified for the amount of macroelements (half-dose) that was supplemented with amino acids (300 mg/l of glutamic acid, 50 mg/l of aspartic acid, 5 mg/l of tyrosine, 3 mg/l of arginine, 2 mg/l of hydroxyproline) and plant growth regulators [2,5 mg/l of 2.4D (2.4-Dichlorophenoxyacetic acid), 0,6 mg/l of BAP (6-Benzyl-aminopurine) and 0,3 mg/l of ABA (Abscisic acid)]. Composition of nutrient medium was developed for regeneration of microplants from callus – agar MS medium was modified for the amount of macroelements (half-dose) supplemented with vitamins: 10 mg/l of thiaminum, 1,0 mg/l of pyridoxine, 1,0 mg/l of nicotinic acid (by White), 1,0 mg/l of ascorbic acid, 250 mg/l of glutamic acid, 2,0 mg/l of BAP, 0,3 mg/l of NAA (Naphthaleneacetic acid). On this medium, 100% regeneration of M. sacchariflorus (Maxim.) Hack and 50% regeneration of M. sinensis Andersson was obtained. Due to media modification aimed at initiating callusogenesis and microplants regeneration, reproduction factor of M. sinensis was increased 20 times at the average, M. sacchariflorus – 35–40 times. Conclusions. Plants of M. sacchariflorus (Maxim.) Hack and M. sinensis Andersson were obtained in vitro culture by initiation of callusogenes and microplants regeneration from the Miscanthus seeds with poor germination and viability on nutrient media of certain composition.

Purpose. To obtain Miscanthus sacchariflorus (Maxim.) Hack and Miscanthus sinensis Andersson in vitro culture by indirect morphogenesis. Methods. Biotechnological procedures, mathematical and statistical analyses. Results. Composition of nutrient medium was developed intended for induction of callusogenesis from Miscanthus seeds with a poor germination and viability of seedlings – Murashige and Skoog (MS) medium was modified for the amount of macroelements (half-dose) that was supplemented with amino acids (300 mg/l of glutamic acid, 50 mg/l of aspartic acid, 5 mg/l of tyrosine, 3 mg/l of arginine, 2 mg/l of hydroxyproline) and plant growth regulators [2,5 mg/l of 2.4D (2.4-Dichlorophenoxyacetic acid), 0,6 mg/l of BAP (6-Benzyl-aminopurine) and 0,3 mg/l of ABA (Abscisic acid)]. Composition of nutrient medium was developed for regeneration of microplants from callus – agar MS medium was modified for the amount of macroelements (half-dose) supplemented with vitamins: 10 mg/l of thiaminum, 1,0 mg/l of pyridoxine, 1,0 mg/l of nicotinic acid (by White), 1,0 mg/l of ascorbic acid, 250 mg/l of glutamic acid, 2,0 mg/l of BAP, 0,3 mg/l of NAA (Naphthaleneacetic acid). On this medium, 100% regeneration of M. sacchariflorus (Maxim.) Hack and 50% regeneration of M. sinensis Andersson was obtained. Due to media modification aimed at initiating callusogenesis and microplants regeneration, reproduction factor of M. sinensis was increased 20 times at the average, M. sacchariflorus – 35–40 times. Conclusions. Plants of M. sacchariflorus (Maxim.) Hack and M. sinensis Andersson were obtained in vitro culture by initiation of callusogenes and microplants regeneration from the Miscanthus seeds with poor germination and viability on nutrient media of certain composition.

Purpose. To select the initial breeding material with complex resistance to Fusarium dry rot and tuber late blight among the created potato of secondary interspecific hyb­rids. Methods. Interspecific hybridization, laboratory test, analytical approach. Results. Based on the interspecific hybridization, the initial breeding material was created and the degree of its resistance to the above pathogens was determined by way of artificial infection of tubers with the inoculum of such fungi as Fusarium sambucinum Fuck and Phytophthora infestans (Mont.) De Bary. During interspecific hybridization based on schemes of saturating and enriching crosses, using forms of various species with a high phenotypic expression of resistance to Fusarium dry rot, the result of the cumulative effect of genes that control resistance to the pathogen was observed. Crossing combinations differed significantly for the degree of population average manifestation of resistance to the diseases. Conclusions. Combinations В54, В53, В61 with a mean resistance (above 7 grades) to Fusarium dry rot have been selected. Such combinations as B52, B50 and B54 had increased resistance to tuber late blight. It was found that the combination В54 is characterized by complex resistance to both diseases. For further work, the following samples with complex resistance to Fusarium dry rot and tuber late blight (7 grades or more) were selected: В59с42, В59с43, В50с16, В50с19, В50с44, В51с1, В51с26, В51с28, В52с11, В52с23, В52с24, В52с29, В53с1, В53с11, В53с17 , В53с23, В54с13, В54с14.

Purpose. To select the initial breeding material with complex resistance to Fusarium dry rot and tuber late blight among the created potato of secondary interspecific hyb­rids. Methods. Interspecific hybridization, laboratory test, analytical approach. Results. Based on the interspecific hybridization, the initial breeding material was created and the degree of its resistance to the above pathogens was determined by way of artificial infection of tubers with the inoculum of such fungi as Fusarium sambucinum Fuck and Phytophthora infestans (Mont.) De Bary. During interspecific hybridization based on schemes of saturating and enriching crosses, using forms of various species with a high phenotypic expression of resistance to Fusarium dry rot, the result of the cumulative effect of genes that control resistance to the pathogen was observed. Crossing combinations differed significantly for the degree of population average manifestation of resistance to the diseases. Conclusions. Combinations В54, В53, В61 with a mean resistance (above 7 grades) to Fusarium dry rot have been selected. Such combinations as B52, B50 and B54 had increased resistance to tuber late blight. It was found that the combination В54 is characterized by complex resistance to both diseases. For further work, the following samples with complex resistance to Fusarium dry rot and tuber late blight (7 grades or more) were selected: В59с42, В59с43, В50с16, В50с19, В50с44, В51с1, В51с26, В51с28, В52с11, В52с23, В52с24, В52с29, В53с1, В53с11, В53с17 , В53с23, В54с13, В54с14.

Мета. Провести типування сортів сої культурної (Glycine max (L.) Merr.) української та закордонної селекції за мікросателітним маркером Satt100 гена Е7 фотоперіодичної чутливості. Методи. Виділення ДНК з проростків на колонках із сорбуючою мембраною; полімеразна ланцюгова реакція; горизонтальний гель-електрофорез; визначення розмірів продуктів ампліфікації за програмою «GelAnalyzer 2010a». Результати. За допомогою аналізу мікросателітного локусу Satt100, який є маркером гена Е7, проведено типування 15 сортів сої. Встановлено наявність продуктів ампліфікації розміром 133, 149 та 168 п.н., що маркують рецесивний та домінантний алелі гена Е7 відповідно. Усі досліджені сорти, крім сорту ‘Одеська 150А’, були гомогенними за мікросателітним локусом Satt100. Генотипи восьми сортів мали рецесивний алель е7, шести – домінантний алель Е7. Порівняли наявність певного алеля гена Е7 у генотипі сорту з належністю до групи стиглості. Відмічено зв’язок для сортів з вегетаційним періодом до 100 діб: усі чотири сорти цієї групи мали алель е7 та для сортів із вегетаційним періодом понад 110 діб: для всіх чотирьох сортів цієї групи характерні фрагменти ампліфікації локусу Satt100, що маркують домінантний алель. Із семи середньо­ранніх сортів чотири мали рецесивний алель е7, а три – домінантний Е7. Наведено інформацію про отримання різними науковцями фрагментів ампліфікації неоднакових розмірів, але при цьому виявлено тенденцію щодо зв’язку більш високомолекулярних фрагментів з домінантним, а низькомолекулярних – з рецесивним алелем. Висновки. За результатами типування за мікросателітним маркером Satt100 гена Е7 фотоперіодичної чутливості для 15 сортів сої визначено наявність рецесивного або домінантного алеля гена Е7. Для більшості зразків встановлено відповідність наявності певного алеля гена Е7 та тривалості вегетаційного періоду. Наявність фрагментів ампліфікації різного розміру, характерних для рецесивного алеля, свідчить про необхідність сиквенування та біоінформатичного аналізу фрагментів ампліфікації локусу Satt100.

Мета. Провести типування сортів сої культурної (Glycine max (L.) Merr.) української та закордонної селекції за мікросателітним маркером Satt100 гена Е7 фотоперіодичної чутливості. Методи. Виділення ДНК з проростків на колонках із сорбуючою мембраною; полімеразна ланцюгова реакція; горизонтальний гель-електрофорез; визначення розмірів продуктів ампліфікації за програмою «GelAnalyzer 2010a». Результати. За допомогою аналізу мікросателітного локусу Satt100, який є маркером гена Е7, проведено типування 15 сортів сої. Встановлено наявність продуктів ампліфікації розміром 133, 149 та 168 п.н., що маркують рецесивний та домінантний алелі гена Е7 відповідно. Усі досліджені сорти, крім сорту ‘Одеська 150А’, були гомогенними за мікросателітним локусом Satt100. Генотипи восьми сортів мали рецесивний алель е7, шести – домінантний алель Е7. Порівняли наявність певного алеля гена Е7 у генотипі сорту з належністю до групи стиглості. Відмічено зв’язок для сортів з вегетаційним періодом до 100 діб: усі чотири сорти цієї групи мали алель е7 та для сортів із вегетаційним періодом понад 110 діб: для всіх чотирьох сортів цієї групи характерні фрагменти ампліфікації локусу Satt100, що маркують домінантний алель. Із семи середньо­ранніх сортів чотири мали рецесивний алель е7, а три – домінантний Е7. Наведено інформацію про отримання різними науковцями фрагментів ампліфікації неоднакових розмірів, але при цьому виявлено тенденцію щодо зв’язку більш високомолекулярних фрагментів з домінантним, а низькомолекулярних – з рецесивним алелем. Висновки. За результатами типування за мікросателітним маркером Satt100 гена Е7 фотоперіодичної чутливості для 15 сортів сої визначено наявність рецесивного або домінантного алеля гена Е7. Для більшості зразків встановлено відповідність наявності певного алеля гена Е7 та тривалості вегетаційного періоду. Наявність фрагментів ампліфікації різного розміру, характерних для рецесивного алеля, свідчить про необхідність сиквенування та біоінформатичного аналізу фрагментів ампліфікації локусу Satt100. | Purpose. Typingsoybean (Glycinemax (L.) Merr.) culti­vars of Ukrainian and foreign breeding for microsatellite mar­ker Satt100 of E7 gene of photoperiod sensitivity. Methods.DNA recover from seedlings on columns with sorbing membrane; polymerase chain reaction (PCR); horizontal gel electro­phoresis; amplification products size determination using “GelAnalyzer 2010a” program. Results. PCR analysis of the microsatellite locus Satt100 to be the marker of the E7 gene was used for typing 15 soybean cultivars. Availability of the 133, 149 and 168 bp amplification products has been established that marked recessive and dominant alleles of Е7 gene correspondingly. All varieties, except ‘Odeska 150A’, were homogeneous for the microsatellite locus Satt100. Genotypes of eight varieties has the recessive allele e7, six ones – the dominant allele E7. Availability of the certain allele of the E7 gene was compared in variety genotype with belonging to the maturity group. The association was found for varieties with vegetation period up to 100 days: all four varieties of this group had the e7 allele, for varieties with vegetation period more than 110 days: for all four varieties of this group the Satt100 locus amplification fragments that mark the dominant allele are specific. Amongsevenmiddle-agedvarieties, fourof them hadtherecessiveallelee7, threeofthem – dominantalleleЕ7. According to thepresented information, some scientistsobtainedamplificationfragments of different size, butthetendency was revealed concerning the association of the higher molecular fragments witht hedominant gene, and the lowmolecular fragments with therecessive gene. Conclusions. Based on the results of typing of 15 soybean varieties for the microsatellite marker Satt100 of E7 gene of photoperiod sensitivity, it was determined availability of recessive or dominant allele of E7 gene. For most samples, the concordance of availability of the certain allele of E7 gene and the duration of the vegetation period was established. Availability of amplification fragments of different size to be specific for recessive allele testified the needfor sequencing and bioinformatic analysis of the Satt100 locus amplification fragments. | Цель. Осуществить типирование сортов сои культурной (Glycine max (L.) Merr.) украинской и зарубежной селекции по микросателлитному маркеру Satt100 гена Е7 фотопериодической чувствительности. Методы. Выделение ДНК из ростков на колонках с сорбирующей мемб­раной; полимеразная цепная реакция; горизонтальный гель-электрофорез; определение размеров продуктов амплификации с использованием программы «GelAnalyzer 2010a». Результаты. С помощью анализа микросателлитного локуса Satt100, являющегося маркером гена Е7, проведено типирование 15 сортов сои. Установлено наличие продуктов амплификации размером 133, 149 и 168 п.н., которые маркируют рецессивный и доминантный аллели гена Е7 соответственно. Все исследуемые сорта, кроме сор­та ‘Одесская 150А’, были гомогенными по микросателлитному локусу Satt100. Генотипы восьми исследованных сор­тов имели рецессивный аллель е7, шести – доминантный аллель Е7. Сравнили наличие определенного аллеля гена Е7 в генотипе сорта с принадлежностью к группе спелости. Отмечено связь для сортов с вегетационным периодом до 100 суток: все четыре сорта этой группы имели аллель е7, и для сортов с вегетационным периодом более 110 суток: для всех четырех сортов этой группы характерны фрагменты амплификации локуса Satt100, которые маркируют доминантный аллель. Среди семи среднеранних сортов четыре имели рецессивный аллель е7, а три – доминантный Е7. Представлено информацию о получении разными учеными фрагментов амплификации неодинаковых размеров, но при этом выявлено тенденцию связи более высокомолекулярного фрагмента с доминантным геном, а низкомолекулярных – с рецессивным геном. Выводы. По результатам типирования по микросателлитному маркеру Satt100 гена Е7 фотопериодической чувствительности для 15 сортов сои определено наличие рецессивного или доминантного аллеля гена Е7. Для большинства образцов установлено соответствие наличия определенного аллеля гена Е7 и продолжительности вегетационного периода. Наличие фрагментов амплификации разного размера, характерных для рецессивного аллеля, свидетельствует о необходимости секвенирования и биоинформатичного анализа фрагментов амплификации локуса Satt100.

Purpose. To analyse the qualitative and quantitative composition of volatile organic substances (VOS) in the petals of six species of dog-rose in the collection of M. M. Gryshko National Botanic Garden of the NAS of Ukraine for further bree­ding. Methods. Volatile organic substances were obtained by steam distillation. VOS was determined using gas chromato­graphy (Chromatograph Agilent Technologies 6890). Results. The qualitative and quantitative composition of volatile organic substances in the petals in such species as Rosa L. (R. roullettii HCh (Correvon), R. multiflora Thunb., R. pimpinellifolia L., R. canina L., R. centifolia L., R. rugosa Thunb.) was investigated. According to the results of the research, 105 VOS were discovered in the dog-rose petals, among which 11 were not identified. The dog-rose petals of identified VOC contained 16 components which share exceeds 5%, 31 – was ranging from 1% to 5%, 47 – was less than 1%. They belonged to different groups including monoterpenoids, sesquiterpenoids, sesquiterpene alcohols, saturated unbranched hydrocarbons etc. The largest number of components were identified in the petals of R. roulettii (49) and R. centifolia (45), while the number of identified components in species R. multiflora (33), R. rugosa (31) and R. canina (30) was relatively smaller. All studied dog-rose species were characterized by the presence of such saturated unbranched hydrocarbons as decan, tetradecane, pentadecane, hexadecane, heptadecane, octadecane, nanodecane, heneicosane, tricosane, tetracosane, pentacosane, heptocosane, hentriacontane. Triterpene hydrocarbon squalene is the important component of the VOS complex in dog-rose pen tals. b-phenylethyl alcohol to be one of the main components of the rose essential oils was found during investigation in the following four dog-rose species as R. centifolia (0.61%), R. pimpinellifolia (3.56%), R. rugosa (4.24%), and R. multiflora (5.43%). Significant content of dihydro-b-ionol (18.46%), dihydro-b-ionone (0.69%), thias­piran A (1.35%), and thiaspiran B (2.17%) were revealed in the petals of R. roulettii, which also have an influence on the aromatic bouquet of the rose oil. Conclusions. For the first time, 105 volatile organic substances were found in the petals of six species of dog-rose, 11 of which were not identified. It was found out that the largest number of components were identified in the petals of R. roulettii (49) and R. centifolia (45). The number of VOS identified in species R. multiflora (33), R. rugosa (31), and R. canina (30) was relatively smaller. Component composition of VOS in the petals of studied dog-rose species is an important constituent in breeding investigations of prospective producers for the various purposes of use.

Purpose. To analyse the qualitative and quantitative composition of volatile organic substances (VOS) in the petals of six species of dog-rose in the collection of M. M. Gryshko National Botanic Garden of the NAS of Ukraine for further bree­ding. Methods. Volatile organic substances were obtained by steam distillation. VOS was determined using gas chromato­graphy (Chromatograph Agilent Technologies 6890). Results. The qualitative and quantitative composition of volatile organic substances in the petals in such species as Rosa L. (R. roullettii HCh (Correvon), R. multiflora Thunb., R. pimpinellifolia L., R. canina L., R. centifolia L., R. rugosa Thunb.) was investigated. According to the results of the research, 105 VOS were discovered in the dog-rose petals, among which 11 were not identified. The dog-rose petals of identified VOC contained 16 components which share exceeds 5%, 31 – was ranging from 1% to 5%, 47 – was less than 1%. They belonged to different groups including monoterpenoids, sesquiterpenoids, sesquiterpene alcohols, saturated unbranched hydrocarbons etc. The largest number of components were identified in the petals of R. roulettii (49) and R. centifolia (45), while the number of identified components in species R. multiflora (33), R. rugosa (31) and R. canina (30) was relatively smaller. All studied dog-rose species were characterized by the presence of such saturated unbranched hydrocarbons as decan, tetradecane, pentadecane, hexadecane, heptadecane, octadecane, nanodecane, heneicosane, tricosane, tetracosane, pentacosane, heptocosane, hentriacontane. Triterpene hydrocarbon squalene is the important component of the VOS complex in dog-rose pen tals. b-phenylethyl alcohol to be one of the main components of the rose essential oils was found during investigation in the following four dog-rose species as R. centifolia (0.61%), R. pimpinellifolia (3.56%), R. rugosa (4.24%), and R. multiflora (5.43%). Significant content of dihydro-b-ionol (18.46%), dihydro-b-ionone (0.69%), thias­piran A (1.35%), and thiaspiran B (2.17%) were revealed in the petals of R. roulettii, which also have an influence on the aromatic bouquet of the rose oil. Conclusions. For the first time, 105 volatile organic substances were found in the petals of six species of dog-rose, 11 of which were not identified. It was found out that the largest number of components were identified in the petals of R. roulettii (49) and R. centifolia (45). The number of VOS identified in species R. multiflora (33), R. rugosa (31), and R. canina (30) was relatively smaller. Component composition of VOS in the petals of studied dog-rose species is an important constituent in breeding investigations of prospective producers for the various purposes of use.

Purpose. To evaluate and select hybrids, varieties of the parental nursery of the breeding process, biotechnological lines and wild species of potato for physiological parameters of drought resistance. Methods. Physiological and biochemical, selection ones, statistical data processing. Results. The data is given concerning the evaluation of the water retaining and water regeneration capacity of potato leaves of promising hybrids of competitive and ecological test, varieties of the parental nursery, biotechnological lines and wild species and their integral indicator of drought resistance. The studied samples were grown in the nurseries of field selection crop rotation. Accordingly, the initial material with the highest drought resistance value has been defined. Among the evaluated material, eight hybrids of the competitive and ecological test have been selected (drought resistance coefficient was ranging from 59.4% to 84.8%) and five biotechnological lines of ‘Hlazurna’ and ‘Dorohin’ varieties (drought resistance coefficient was in the range of 55.5% to 67.5%). As for wild species, almost half of the samples (47.8%) were characterized by a high coefficient of drought resistance (from 55 to 78%). Selected samples with high values of drought resistance were recommended to use as a source and drought resistance donors when creating new potato varieties. Conclusions. The initial potato material (hybrids, varieties, biotechnological lines and wild species) with high values of drought resistance (55.0–84.8%) has been selected. These samples are recommended to use in the breeding process when creating new drought resistance potato varieties.

Purpose. To evaluate and select hybrids, varieties of the parental nursery of the breeding process, biotechnological lines and wild species of potato for physiological parameters of drought resistance.Methods. Physiological and biochemical, selection ones, statistical data processing.Results. The data is given concerning the evaluation of the water retaining and water regeneration capacity of potato leaves of promising hybrids of competitive and ecological test, varieties of the parental nursery, biotechnological lines and wild species and their integral indicator of drought resistance. The studied samples were grown in the nurseries of field selection crop rotation. Accordingly, the initial material with the highest drought resistance value has been defined. Among the evaluated material, eight hybrids of the competitive and ecological test have been selected (drought resistance coefficient was ranging from 59.4% to 84.8%) and five biotechnological lines of ‘Hlazurna’ and ‘Dorohin’ varieties (drought resistance coefficient was in the range of 55.5% to 67.5%). As for wild species, almost half of the samples (47.8%) were characterized by a high coefficient of drought resistance (from 55 to 78%). Selected samples with high values of drought resistance were recommended to use as a source and drought resistance donors when creating new potato varieties.Conclusions. The initial potato material (hybrids, varieties, biotechnological lines and wild species) with high values of drought resistance (55.0–84.8%) has been selected. These samples are recommended to use in the breeding process when creating new drought resistance potato varieties. | Мета. Оцінити та провести добір гібридів, сортів батьківського розсадника селекційного процесу, біотехнологічних ліній та диких видів картоплі за фізіологічними показниками посухостійкості.Методи. Фізіолого-біохімічні, селекційні, статистичні.Результати. Наведено дані оцінювання водоутримувальної та водовідновлювальної здатності листків картоплі перспективних гібридів конкурсно-екологічного випробування, сортів батьківського розсадника, біотехнологічних ліній і диких видів та їх інтегрального показника посухостійкості. Досліджувані зразки вирощували в розсадниках польової селекційної сівозміни. Відповідно визначено вихідний матеріал з найвищим показником посухостійкості. З-поміж оцінюваного матеріалу виділено: вісім гібридів конкурсно-екологічного випробування – коефіцієнт посухостійкості коливався від 59,4 до 84,8%, п’ять біотехнологічних ліній сортів ‘Глазурна’ та ‘Дорогинь’ – коефіцієнт посухостійкості у межах від 55,5 до 67,5%. Серед диких видів майже половина зразків (47,8%) мала високий коефіцієнт посухостійкості – від 55 до 78%. Зразки з високими показниками посухостійкості рекомендовано використовувати як джерело та донори стійкості до посухи під час створення нових посухостійких сортів картоплі.Висновки. Виділено вихідний матеріал картоплі (гібриди, сорти, біотехнологічні лінії та дикі види) з високим коефіцієнтом посухостійкості (55,0–84,8%). Рекомендується використовувати ці зразки у селекційному процесі під час створення посухостійких сортів картоплі. | Цель. Оценить и провести отбор гибридов, сортов родительского питомника селекционного процесса, биотехнологических линий и диких видов картофеля по физиологичес­ким показателям засухоустойчивости. Методы. Физиолого-биохимические, селекционные, статистические. Результаты. Приведены данные оценивания водоудерживающей и водообновляющей способности листьев картофеля перспективных гибридов конкурсно-экологического испытания, сортов родительского питомника, биотехнологических линий, диких видов и их интегрального показателя засухоустойчивости. Исследуемые образцы выращивали в питомниках полевого селекционного севооборота. Соответственно определен исходный материал с наивысшим показателем засухоустойчивости. Среди оцениваемого материала выделено: восемь гибридов конкурсно-экологического испытания – коэффициент засухоустойчивости колебался от 59,4 до 84,8%, пять биотехнологических линий сортов ‘Глазурна’ и ‘Дорогинь’ с коэффициентом засухоустойчивости в пределах от 55,5 до 67,5%. Среди диких видов почти половина образцов (47,8%) имели высокий коэффициент засухоустойчивости – от 55 до 78%. Образцы с высокими показателями засухоустойчивости рекомендовано использовать как источники и доноры устойчивости к засухе при создании новых засухоустойчивых сортов картофеля. Выводы. Выделен исходный материал картофеля (гибриды, сорта, биотехнологические линии и дикие виды) с высоким коэффициентом засухоустойчивости (55,0–84,8%). Рекомендовано использовать эти образцы в селекционном процессе при создании засухоустойчивых сор­тов картофеля.

Purpose. To evaluate and select hybrids, varieties of the parental nursery of the breeding process, biotechnological lines and wild species of potato for physiological parameters of drought resistance. Methods. Physiological and biochemical, selection ones, statistical data processing. Results. The data is given concerning the evaluation of the water retaining and water regeneration capacity of potato leaves of promising hybrids of competitive and ecological test, varieties of the parental nursery, biotechnological lines and wild species and their integral indicator of drought resistance. The studied samples were grown in the nurseries of field selection crop rotation. Accordingly, the initial material with the highest drought resistance value has been defined. Among the evaluated material, eight hybrids of the competitive and ecological test have been selected (drought resistance coefficient was ranging from 59.4% to 84.8%) and five biotechnological lines of ‘Hlazurna’ and ‘Dorohin’ varieties (drought resistance coefficient was in the range of 55.5% to 67.5%). As for wild species, almost half of the samples (47.8%) were characterized by a high coefficient of drought resistance (from 55 to 78%). Selected samples with high values of drought resistance were recommended to use as a source and drought resistance donors when creating new potato varieties. Conclusions. The initial potato material (hybrids, varieties, biotechnological lines and wild species) with high values of drought resistance (55.0–84.8%) has been selected. These samples are recommended to use in the breeding process when creating new drought resistance potato varieties.

Purpose. To define spring barley breeding lines with an optimal combination of yielding capacity and stability under different weather conditions in the Central Forest-Steppe zone of Ukraine. Methods. Field studies, ANOVA, AMMI, GGE biplot analysis. Results. Hydrothermal regime during interphase periods of spring barley vegetation under conditions of The V. M. Remeslo Myronivka Institute of Wheat of NAAS in 2012–2014 was characterized by significant variability, that facilitated detailed evaluation of the lines for productivity, stability, and resistance to abiotic and biotic factors. In the performance of an experiment, the highest average yielding capacity (5.87 t/ha) was noted in 2012, the lowest one (3.50 t/ha) was in 2013. As for these years, average yield of 4.63 t/ha was obtained in 2014. By applying AMMI and GGE biplot analysis, significant differences in response of the studied lines to variability of weather conditions was revealed. Using AMMI model, additive components of the main effects of the breeding lines and years of testing as well as multiplicative components of their interaction were characterized. GGE biplot genotypes ranking in relation to a hypothetical “ideal” genotype showed an absolute advantage of breeding line ‘Nutans 4540’ for yielding capacity and stability. In addition, breeding lines ‘Nutans 4241’ and ‘Nutans 4120’ were close to ideatype. Selected breeding lines were characterized by resistance and moderate resistance to leaf diseases and lodging. Conclusions. Use of AMMI and GGE biplot analysis to evaluate breeding lines at the final stages of breeding process allows to describe them thoroughly and graphically as well as differentiate them not only for average yielding capacity but also for their interaction with changing conditions during the years of testing. Breeding lines ‘Nutans 4540’, ‘Nutans 4241’, and ‘Nutans 4120’ to be identified for the stability of yielding capacity display in combination with other agronomic characters were transferred to the State variety testing as new spring barley varieties ‘MIP Myrnyi’, ‘MIP Saliut’, and ‘MIP Sotnyk’ respectively.

Purpose. To define spring barley breeding lines with an optimal combination of yielding capacity and stability under different weather conditions in the Central Forest-Steppe zone of Ukraine. Methods. Field studies, ANOVA, AMMI, GGE biplot analysis. Results. Hydrothermal regime during interphase periods of spring barley vegetation under conditions of The V. M. Remeslo Myronivka Institute of Wheat of NAAS in 2012–2014 was characterized by significant variability, that facilitated detailed evaluation of the lines for productivity, stability, and resistance to abiotic and biotic factors. In the performance of an experiment, the highest average yielding capacity (5.87 t/ha) was noted in 2012, the lowest one (3.50 t/ha) was in 2013. As for these years, average yield of 4.63 t/ha was obtained in 2014. By applying AMMI and GGE biplot analysis, significant differences in response of the studied lines to variability of weather conditions was revealed. Using AMMI model, additive components of the main effects of the breeding lines and years of testing as well as multiplicative components of their interaction were characterized. GGE biplot genotypes ranking in relation to a hypothetical “ideal” genotype showed an absolute advantage of breeding line ‘Nutans 4540’ for yielding capacity and stability. In addition, breeding lines ‘Nutans 4241’ and ‘Nutans 4120’ were close to ideatype. Selected breeding lines were characterized by resistance and moderate resistance to leaf diseases and lodging. Conclusions. Use of AMMI and GGE biplot analysis to evaluate breeding lines at the final stages of breeding process allows to describe them thoroughly and graphically as well as differentiate them not only for average yielding capacity but also for their interaction with changing conditions during the years of testing. Breeding lines ‘Nutans 4540’, ‘Nutans 4241’, and ‘Nutans 4120’ to be identified for the stability of yielding capacity display in combination with other agronomic characters were transferred to the State variety testing as new spring barley varieties ‘MIP Myrnyi’, ‘MIP Saliut’, and ‘MIP Sotnyk’ respectively.

Purpose. To investigate features of commercial head quality formation in varieties of cutting lettuce (Lactuca sativa var. capitata L.) for different growing methods. To study the influence of growing methods for cutting lettuce plants on the weight of commercial heads, indices of yield and quality of freshly harvested products. Methods. Field study, laboratory testing and statistical evaluation. Results. The results of studying peculiarities of head formation in such cutting lettuce varieties as ‘Godar’ (control), ‘Smuhlianka’, ‘Olzhych’ and ‘Dyvohrai’ were shown. Based on the results of phenological observations, the duration of interstage periods of growth and development of cutting lettuce plants was determined for the different growing methods. The model of cutting lettuce of capitata variety for different growing methods was presented in terms of biochemical parameters (content of dry matter, vitamin C, nitrates, amount of sugars). The influence of growing methods for cutting lettuce plants on the commercial head weight, indices of yield and quality of freshly harvested products was studied. Obtained results confirmed that in early ripening varie­ti­es the heads were smaller (150 g) and not dense, in late ripening varieties they were large and dense, weight was 150–500 g. Freshly harvested commercial products of head lettuce in case of seedling cultivation was characterized by a slight increase in dry matter content by 0.15–0.29%. The corresponding pattern was observed for the total sugar content. Its amount was slightly higher: 1.3–1.9% in case of direct sowing and 1.6–1.9% – for seedlings cultivation. The content of ascorbic acid (vitamin C, mg/100 g) was the highest in the ‘Dyvohrai’ variety despite of the method of growing. The largest quantity of nitrates in lettuce heads was in the inner stem (480 mg/kg), while in the middle of the head it was reduced to 110 mg/kg. Conclusions. Freshly harvested heads of cutting lettuce were analyzed for the content of such basic biochemical indices as dry matter, vitamin C, protein, nitrates, amount of sugars. Biochemical characteristics of freshly harvested products of heading lettuce in case of direct sowing and non-seedling methods of growing were within the error being almost identical with minor deviations. According to the study results, a typical model of capitate lettuce variety was designed which will have practical application in qualification examination of plant varieties.

Purpose. To investigate features of commercial head quality formation in varieties of cutting lettuce (Lactuca sativa var. capitata L.) for different growing methods. To study the influence of growing methods for cutting lettuce plants on the weight of commercial heads, indices of yield and quality of freshly harvested products. Methods. Field study, laboratory testing and statistical evaluation. Results. The results of studying peculiarities of head formation in such cutting lettuce varieties as ‘Godar’ (control), ‘Smuhlianka’, ‘Olzhych’ and ‘Dyvohrai’ were shown. Based on the results of phenological observations, the duration of interstage periods of growth and development of cutting lettuce plants was determined for the different growing methods. The model of cutting lettuce of capitata variety for different growing methods was presented in terms of biochemical parameters (content of dry matter, vitamin C, nitrates, amount of sugars). The influence of growing methods for cutting lettuce plants on the commercial head weight, indices of yield and quality of freshly harvested products was studied. Obtained results confirmed that in early ripening varie­ti­es the heads were smaller (150 g) and not dense, in late ripening varieties they were large and dense, weight was 150–500 g. Freshly harvested commercial products of head lettuce in case of seedling cultivation was characterized by a slight increase in dry matter content by 0.15–0.29%. The corresponding pattern was observed for the total sugar content. Its amount was slightly higher: 1.3–1.9% in case of direct sowing and 1.6–1.9% – for seedlings cultivation. The content of ascorbic acid (vitamin C, mg/100 g) was the highest in the ‘Dyvohrai’ variety despite of the method of growing. The largest quantity of nitrates in lettuce heads was in the inner stem (480 mg/kg), while in the middle of the head it was reduced to 110 mg/kg. Conclusions. Freshly harvested heads of cutting lettuce were analyzed for the content of such basic biochemical indices as dry matter, vitamin C, protein, nitrates, amount of sugars. Biochemical characteristics of freshly harvested products of heading lettuce in case of direct sowing and non-seedling methods of growing were within the error being almost identical with minor deviations. According to the study results, a typical model of capitate lettuce variety was designed which will have practical application in qualification examination of plant varieties.