In this paper, Taguchi method was applied to determine the optimum condition for Pb (II) removal from aqueous solution by spent Agaricus bisporus. An orthogonal array experiment design (L₉(3⁴) which is of four control factors (pH, t (contact time), m (sorbent mass), and C ₀ (initial Pb (II) concentration)) having three levels was employed. Biosorption capacity (mg metal/g biosorbent) and percent removal (%) were investigated as the quality characteristics to be optimized. In order to determine the optimum levels of the control factors precisely, range analysis and analysis of variance were performed. The optimum condition for biosorption capacity was found to be pH = 5.00, t = 5.0 h, m = 0.010 g, and C ₀ = 50 mg/L. And for percent removal, the optimum condition was found to be pH = 4.00, t = 4.0 h, m = 0.100 g, and C ₀ = 50 mg/L. Under these optimum conditions, biosorption capacity and percent removal can reach 60.76 mg/g and 80.50%, respectively.

To investigate the remediation potential of spent mushroom substrate (SMS) on Cd pollution in a paddy soil, a rice pot experiment was conducted to study the effects of SMS addition on the availability of Cd in soil and the uptake of Cd in rice tissues. Five percent of SMS from Pleurotus eryngii (SMS-A, treatment: A), SMS from Agaricus bisporus (SMS-B, treatment: B), or SMS-A plus SMS-B (1:1, treatment: A+B) were added into a Cd-contaminated paddy soil before planting, respectively. The treatment of no SMS amendment was set up as the control (CK). At the four main growth stages of rice, the soils and plant samples were collected to detect the soil properties, Cd concentration in soils and rice tissues, and Cd fractions in soils. Results indicated that the application of SMS-A, SMS-B, and A+B significantly increased soil pH by 14.0–22.9, 23.9–32.9, and 22.7–30%, organic matter (OM) contents by 12.9–31.5, 22.1–34.5, and 26.1–36.9% comparing with CK. While cation exchange capacities (CECs) were increased by 3.6–8.5, 4.9–13.1, and 0.4–10.0% in A, B, and A+B treatments, respectively, except those at the maturation stage in A and B treatments. However, the CaCl₂-Cd concentrations in soils were significantly decreased by 64.8–77.9, 76.1–98.9, 73.2–98.9% in A, B, and A+B treatments, respectively, comparing with CK. The reduced availability of Cd was attributed to the changes of Cd from soluble to insoluble fractions in soils amended with SMS and resulted in the decreased Cd uptake in rice tissues. The Cd concentrations in roots significantly decreased by 22.8–36.9, 28.6–36.6, and 26.8–42.6%, while the Cd concentrations in straw decreased by 20.1–46.4, 9.3–41.6, and 16.0–49.1% in A, B, and A+B treatments, respectively. At the maturation stage, the Cd concentrations in brown rice were reduced by 17.7, 15.9, and 19.4% in A, B, and A+B treatments, respectively. Correlation analysis revealed that the Cd concentrations in rice roots, straws, and brown rice were all positively correlated with CaCl₂-Cd concentrations of soils. Moreover, soil pH and OM were significantly negatively correlated with the Cd concentration in rice tissues, except that between soil pH and the Cd concentration in rice straws. Therefore, the reduced Cd availability in soil and uptake in rice plant tissues together with better soil nutrient conditions by SMS application improved the biomass of root and straw at heading, filling, and maturation stages and the rice production by 32.9–38.8% at the maturation stage. The combined application of SMS-A and SMS-B can be used as a potential method for remediation of Cd-contaminated paddy soil.

This study explored the sustainable use of treated sugar mill wastewater (SMW) to cultivate the White button (Agaricus bisporus J.E. Lange) mushroom and the attendant risk of trace metals accumulated in the fruiting bodies. The wheat straw substrate was loaded with a normal water supply and different doses of SMW to enhance its moisture and nutrient contents. The impact of the SMW amendment on A. bisporus yield, biological efficiency, and spawn-running time was assessed. Furthermore, the substrate properties (pH, organic matter, total nitrogen, total phosphorus, etc.) based prediction models for trace metal uptake by A. bisporus fruiting bodies were developed using multiple linear regression (MLR) and artificial neural network (ANN) approaches. The results showed that maximum A. bisporus yield (158.42 ± 8.74 g/kg fresh substrate), biological efficiency (105.61 ± 3.97%), and minimum time of spawn-running (15 days) were observed in 75% SMW enrichment. For the prediction of Cd, Cu, Cr, Fe, Mn, and Zn trace metal uptake, the ANN models showed better performance in terms of R² (> 0.995), root means square error (RMSE < 0.075), model efficiency (ME > 0.99), and model normalized bias (MNB < 0.009), as compared with those of MLR models with R² (0.972), RMSE (< 0.441), ME (> 0.96), and MNB (< 0.034), respectively. On the other hand, the target hazard quotient (THQ) showed no significant health risk associated with the consumption of trace metal-contaminated A. bisporus in both adult and child groups. Thus, the findings of this study present a novel, safe, and sustainable method of A. bisporus cultivation along with treated agro-based wastewater management.

High doses of lithium salts are used for the treatment or prevention of episodes of mania in bipolar disorder, but the medication is rapidly excreted and also shows side effects. Li may also be beneficial in people with mood disorders. Nutritionally, popular foods such as wild and cultivated mushrooms have low Li contents. This study evaluated the Li enrichment of white Agaricus bisporus mushrooms using Li₂CO₃ solutions to fortify the commercial growing substrate at various concentrations from 1.0 to 500 mg kg⁻¹ dry weight (dw). Fortification of up to 100 mg kg⁻¹ dw resulted in a significant (p < 0.01) dose-dependent increase in the accumulation of Li in mushroom, but the highest fortification level was found to be detrimental to fruitification. The median values of Li in fortified mushrooms corresponded to the fortification levels, increasing from 0.49 to 17 mg kg⁻¹ dw relative to the background concentration of 0.056 mg kg⁻¹ dw (control substrate contained 0.10 mg kg⁻¹ dw). The potential for Li uptake in fruiting bodies was found to decrease at higher levels of fortification, with saturation occurring at 100 mg kg⁻¹. Resulting lithiated mushrooms were up to 300-fold richer in Li content than specimens grown on control substrate. The fortification showed some effects on the uptake of other trace minerals, but concentrations of co-accumulated Ag, Al, As, Ba, Cd, Co, Cr, Cs, Cu, Hg, Mn, Ni, Pb, Rb, Sr, Tl, U, V and Zn were similar or lower than values reported in the literature for commercial A. bisporus. These lithiated mushrooms could be considered as a pro-medicinal alternative to treatments that use Li salts.

Bioremediation of mixed metal–organic soil pollution constitutes a difficult task in different ecosystems all around the world. The aims of this work are to determine the capacity of two spent mushroom substrates (Agaricus bisporus and Pleurotus ostreatus) to immobilize Cd and Pb, to assess the effect of these metals on laccase activity, and to determine the potential of spent A. bisporus substrate to biodegrade four polycyclic aromatic hydrocarbons (PAH): fluorene, phenanthrene, anthracene, and pyrene, when those toxic heavy metals Cd and Pb are present. According to adsorption isotherms, spent P. ostreatus and A. bisporus substrates showed a high Pb and Cd adsorption capacity. Pb and Cd interactions with crude laccase enzyme extracts from spent P. ostreatus and A. bisporus substrates showed Cd and Pb enzyme inhibition; however, laccase activity of A. bisporus presented lower inhibition. Spent A. bisporus substrate polluted with PAH and Cd or Pb was able to biodegrade PAH, although both metals decrease the biodegradation rate. Spent A. bisporus substrate contained a microbiological consortium able to oxidize PAH with high ionization potential. Cd and Pb were immobilized during the bioremediation process by spent A. bisporus substrate. Consequently, spent A. bisporus substrate was adequate as a multi-polluted soil bioremediator.

Providing guidance on the reasonable use of pesticide in agricultural production is of particular importance for ensuring food safety. In the present study, a field trial was performed to study the dissipation and accumulative pattern of cyromazine (CA) and its metabolite in Agaricus bisporus (A. bisporus) cultivation. An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was first developed and validated for the determination of CA and melamine (MEL) in the casing soil and fruiting body. During the cultivation period, the dissipation rates of CA in the casing soil were between 51.57 and 63.48% at three dose groups. The fruiting body presented higher accumulation ability for MEL compared with CA. The terminal residues of MEL never exceeded the maximum residue limits (MRLs) in food. In addition, the intake health risk from the CA and MEL residues in the fruiting body were negligible to humans. This study will help to provide valuable guidance on the application strategies of CA in A. bisporus cultivation.

INTRODUCTION: A biosorbent was developed by simple dried Agaricus bisporus (SDAB) and effectively used for the biosorption of cationic dyes, Crystal Violet and Brilliant Green. MATERIALS AND METHODS: For the evaluation of the biosorbent system, all the batch equilibrium parameters like pH, biomass dose, contact time, and temperature were optimized to determine the decolorization efficiency of the biosorbent. The maximum yields of dye removal were achieved at pH 4.0 for Crystal Violet (CV) and pH 5.0 for Brilliant Green (BG), which are closer to their natural pH also. RESULT AND DISCUSSION: Equilibrium was established at 60 and 40 min for CV and BG, respectively. Pseudo first-order, pseudo second-order, and intraparticle-diffusion kinetic models were studied at different temperatures. Isotherm models such as Freundlich, Langmuir, and Dubinin–Radushkevich were also studied. Biosorption processes were successfully described by Langmuir isotherm model and the pseudo second-order kinetic model. CONCLUSIONS: The biosorption capacity of A. bisporus over CV and BG were found as 21.74 and 12.16 mg gm⁻¹. Thermodynamic parameters indicated that the CV and BG dye adsorption onto A. bisporus is spontaneous and exothermic in the single and ternary systems. Scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy were used for the surface morphology, crystalline structure of biosorbent, and dye–biosorbent interaction, respectively. This analysis of the biosorption data confirmed that these biosorption processes are ecofriendly and economical. Thus, this biomass system may be useful for the removal of contaminating cationic dyes.