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Testing for heterotopia formation in rats after developmental exposure to selected in vitro inhibitors of thyroperoxidase
2021
Ramhøj, Louise | Frädrich, Caroline | Svingen, Terje | Scholze, Martin | Wirth, Eva K. | Rijntjes, Eddy | Köhrle, Josef | Kortenkamp, Andreas | Axelstad, Marta
The thyroperoxidase (TPO) enzyme is expressed by the thyroid follicular cells and is required for thyroid hormone synthesis. In turn, thyroid hormones are essential for brain development, thus inhibition of TPO in early life can have life-long consequences for brain function. If environmental chemicals with the capacity to inhibit TPO in vitro can also alter brain development in vivo through thyroid hormone dependent mechanisms, however, remains unknown. In this study we show that the in vitro TPO inhibiting pesticide amitrole alters neuronal migration and induces periventricular heterotopia; a thyroid hormone dependent brain malformation. Perinatal exposure to amitrole reduced pup serum thyroxine (T4) concentrations to less than 50% of control animals and this insufficiency led to heterotopia formation in the 16-day old pup’s brain. Two other in vitro TPO inhibitors, 2-mercaptobenzimidazole and cyanamide, caused reproductive toxicity and had only minor sporadic effects on the thyroid hormone system; consequently, they did not cause heterotopia. This is the first demonstration of an environmental chemical causing heterotopia, a brain malformation until now only reported for rodent studies with the anti-thyroid drugs propylthiouracil and methimazole. Our results highlight that certain TPO-inhibiting environmental chemicals can alter brain development through thyroid hormone dependent mechanisms. Improved understanding of the effects on the brain as well as the conditions under which chemicals can perturb brain development will be key to protect human health.
Показать больше [+] Меньше [-]Photooxidation of herbicide amitrole in the presence of fulvic acid
2018
Pozdnyakov, Ivan P. | Sherin, Peter S. | Salomatova, Victoria A. | Parkhats, Marina V. | Grivin, Vjacheslav P. | Dzhagarov, Boris M. | Bazhin, Nikolai M. | Plyusnin, Victor F.
Fulvic acid (Henan ChangSheng Corporation) photoinduced degradation of non-UVA-absorbing herbicide amitrole (3-amino-1,2,4-triazole, AMT) as a way for its removal from polluted water was investigated in details. It was shown that the main primary species generated by fulvic acid under UVA radiation, triplet state and hydrated electron, are not directly involved in the herbicide degradation. AMT decays in reactions with secondary intermediates, reactive oxygen species, formed in reactions of the primary ones with dissolved oxygen. Singlet oxygen is responsible for 80% of herbicide oxidation, and •OH and O₂⁻• radicals—for the remaining 20% of AMT. It was found that quantum yield of AMT photodegradation (ϕ ³⁶⁵ⁿᵐ) decreases linearly from 2.2 × 10⁻³ to 1.2 × 10⁻³ with the increase of fulvic acid concentration from 1.1 to 30 mg L⁻¹. On the contrary, the increase of AMT concentration from 0.8 to 25 mg L⁻¹ leads to practically linear growth of ϕ ³⁶⁵ⁿᵐ value from 1.8 × 10⁻⁴ to 4 × 10⁻³. Thus, the fulvic acid exhibits a good potential as UVA photooxidizer of organic pollutants sensitive to the singlet oxygen (ϕ ⁵³²ⁿᵐ(¹O₂) = 0.025 at pH 6.5).
Показать больше [+] Меньше [-]Part II: temporal and spatial distribution of multiclass pesticide residues in lake sediments of northern Greece: application of an optimized MAE-LC-MS/MS pretreatment and analytical method
2014
Kalogridi, Eleni-Chrysoula | Christophoridis, Christophoros | Bizani, Erasmia | Drimaropoulou, Garyfallia | Fytianos, Konstantinos
The development and application of an analytical methodology for the pretreatment and determination of 253 multiclass pesticides, in lake sediment samples, using liquid chromatography coupled with mass spectrometry (LC-MS/MS) are described in this work. Sediments of lakes Volvi, Doirani, and Kerkini, located in northern Greece, were collected in two-time periods (fall/winter 2010 and spring/summer 2011) and analyzed, applying the developed analytical methodology. Microwave-assisted extraction (MAE) was applied to extract the pesticide residues from lake sediment samples. Analytical results were stored, categorized, and visualized using geographical information systems, in order to assess and observe spatial and temporal variations of the pollution. Main pesticides that were detected included the following: amitrole, tebuconazole, phoxim, diniconazole, sethoxydim, temephos, tetrachlorvinphos, pendimethalin, boscalid, disulfoton sulfone, lenacil, propiconazole, cycloxydim, pyridaben, and terbuthylazine. Amitrole, diniconazole, and tebuconazole were found to be common in all three lakes. Lakes Kerkini and Doirani exhibited increased concentrations during the first sampling period (winter 2010) with predominant pesticide classes, triazines/triazoles and organophosphates. Pollution is mainly located near the populated villages of the lakes and the nearby cultivations. During the second sampling period, pesticide concentrations appear lower and located in sediments near the center of the lake. Lake Volvi exhibits increased pesticide concentrations during the second sampling period, temporal and spatial variations and different pesticide profile pattern. Increased pollution occurs near the center of the lake during the first sampling period, mainly comprised by triazines/triazoles and organophosphates. During the second sampling period, the majority of the sediment samples demonstrated a different pesticide profile dominated by unclassified pesticides and triazines/triazoles. Mineralogical analysis of the samples demonstrates that sediments are mainly composed of clay, mud, and sand particles, and they present spatial variations. Near the center of the lakes, sediments appear to be more fine-grained with higher clay content and are more likely to adsorb pesticides.
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