Currently, 1.3 billion tonnes of food are thrown away each year, most of which are incinerated or landfilled causing large environmental, social, and economic issues. Therefore, the utilisation of food waste as biofertilisers, such as composts and digestates, is a solution to reduce the problems created by incineration and landfilling whilst simultaneously amending soils. The improper disposal of food wastes and bulking materials can contribute to high levels of contaminants within the end-product. Moreover, the food waste and bulking materials, themselves, may contain trace amounts of contaminants. These contaminants tend to have long half-lives, are easily mobile within soil and plants, can accumulate within the food supply chain, and have moderate to high levels of toxicity. This review aims to examine the current and emerging contaminants of high concern that impact the quality of food-waste fertilisers. The paper presents the volume of current and emerging contaminants of plastics, other physical (particulate) contaminants, heavy metals, pesticides, polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), per- and polyfluoroalkyl substances (PFAS), and pathogens within food-waste composts and digestates. Due to the large extent of organic chemical contaminants and the unknown level of toxicity and persistence, the risk assessment of organic chemical contaminants in the food-supply chain remains largely unknown. This study has presented available data from literature of various contaminants found in food waste, and composts and digestates derived from food waste, and evaluated the data with current regulations globally. Overall, to reduce contaminants in composts and digestates, more studies are required on the implementation of proper disposal separation, effective composting and digestion practices, increased screening of physical contaminants, development of compostable plastics, and increased regulatory policies on emerging, problematic contaminants. Moreover, examination of emerging contaminants in food-waste composts and digestates is needed to ensure food security and reduce future human-health risks.

Land application of sewage sludge is increasingly used as an alternative to landfilling and incineration owing to a considerable content of carbon and essential plant nutrients in sewage sludge. However, the presence of chemical and biological contaminants in sewage sludge poses potential dangers; therefore, sewage sludge must be suitably treated before being applied to soils. The most common methods include anaerobic digestion, aerobic composting, lime stabilization, incineration, and pyrolysis. These methods aim at stabilizing sewage sludge, to eliminate its potential environmental pollution and restore its agronomic value. To achieve best results on land, a comprehensive understanding of the transformation of organic matter, nutrients, and contaminants during these sewage-sludge treatments is essential; however, this information is still lacking. This review aims to fill this knowledge gap by presenting various approaches to treat sewage sludge, transformation processes of some major nutrients and pollutants during treatment, and potential impacts on soils. Despite these treatments, overtime there are still some potential risks of land application of treated sewage sludge. Potentially toxic substances remain the main concern regarding the reuse of treated sewage sludge on land. Therefore, further treatment may be applied, and long-term field studies are warranted, to prevent possible adverse effects of treated sewage sludge on the ecosystem and human health and enable its land application.

Microplastic beads are an emerging contaminant that can cause serious environmental and public health problems. Potential bypass of microplastic beads from wastewater to sludge treatment systems is a key challenge in the conventional wastewater treatment process. Moreover, there are no systematic studies on microplastic bead degradation by hydrolytic enzymes that are rich in concentration within wastewater and sludge treatment processes (e.g., anaerobic digestion (AD)). In this study, lab-scale experiments were conducted to investigate the degradation of high-density polyethylene beads by hydrolytic enzymes (e.g., lipase) under various experimental conditions (e.g., temperature). In a 3-day batch experiment, protease was most effective in polyethylene bead degradation as 4.0% of the initial bead mass was removed at an enzyme concentration of 88 mg/L under thermophilic temperature (55 °C). It was also found that the increasing enzyme concentration and high temperature enhanced the polyethylene bead degradation. In a separate 7-day experiment with repeated doses of protease, 23.3% of the initial mass of beads was removed at thermophilic temperature, indicating that AD with a long retention time (e.g., 20 days) and heated temperature has a significant potential for polyethylene bead degradation. A mathematical model was developed and calibrated using the experimental results to estimate the kinetic constant of the high-density polyethylene bead reduction by an enzyme (k1,i) and enzyme self-decay constant (k2,ii). The calibrated k1,i ranged from 5.0 to 8.1× 10⁻⁴ L/mg/hr while k2,ii was 0.44–1.10 L/mg/hr. Using the calibrated model, degradation of polyethylene beads using a mixture of cellulase and protease was simulated, considering an interactive-decay reaction between the two enzymes. The calibrated model was used to simulate the polyethylene bead degradation in AD where 70–95% of the initial bead mass was removed at typical retention time under mesophilic digestion (37.5 °C). Based on the experimental and simulation results, it can be concluded that hydrolytic enzymes can be an efficient technology for large-scale high-density polyethylene bead removal applications.

The environmental risks of microplastics (MPs) have raised an increasing concern. However, the effects of MPs in anaerobic digestion (AD) systems of waste activated sludge (WAS), especially on the fate of antibiotic resistance genes (ARGs), have not been clearly understood. Herein, the variation and interaction of digestion performance, microbial communities and ARGs during AD process of WAS in the presence of polyethylene (PE) MPs with two sizes, PE MPs-180μm and PE MPs-1mm, were investigated. The results showed that the presence of PE MPs, especially PE MPs-1mm, led to the increased hydrolysis of soluble polysaccharides and proteins and the accumulation of volatile fatty acids. The methane production decreased by 6.1% and 13.8% in the presence of PE MPs-180μm and PE MPs-1mm, respectively. Together with this process, hydrolytic bacteria and acidogens were enriched, and methanogens participating in acetoclastic methanogenesis were reduced. Meanwhile, ARGs were enriched obviously by the presence of PE MPs, the abundances of which in PE MPs-180μm and PE MPs-1mm groups were 1.2–3.0 times and 1.5–4.0 times higher than that in the control by the end of AD. That was associated with different co-occurrence patterns between ARGs and bacterial taxa and the enrichment of ARG-hosting bacteria caused by the presence of PE MPs. Together these results suggested the adverse effects of PE MPs on performance and ARGs removal during AD process of WAS through inducing the changes of microbial populations.

PURPOSE OF REVIEW: Organic solid wastes (OSWs) have great potential for resourceful applications. However, individual treatment technologies are difficult to effectively recover their resources. This review aims to describe the development of solid-state anaerobic digestion (SS-AD), digestate aerobic composting, and their hybrid technology (SSADAC) for OSWs treatment to maximize resource recovery from OSWs. RECENT FINDINGS: SSADAC exhibits high potential for OSW treatment in energy and nutrient recovery. As individual treatment technologies, SS-AD and digestate composting recover energy and nutrients in terms of methane and compost, respectively. However, some deficiencies of these individual treatment technologies are hard to be ignored, such as energy loss and liquid digestate/leaching discharge. SSADAC can alleviate these issues with fully synergizing the characteristics of two treatment units for multi-target products. Thus, recent studies have proposed that the regulation of digestion duration can improve SSADAC performance, and other potential methods can also improve the value of SSADAC, such as raw material regulation and exogenous additives, to achieve zero waste discharge and maximum resource recovery. This review presents the applications of SS-AD and digestate composting for OSW treatment and illustrates the development and potential improvements of SSADAC as an integrated process. Key issues and their potential counter-measurements were displayed to provide the further development of SSADAC.

This study used biogas residue produced by anaerobic fermentation of food waste as the raw material in large-scale windrow composting. The effects of the addition of a microbial consortium on the physical and chemical properties and stability of composting of biogas residue were studied. The maturity of food waste biogas residue during composting was investigated by multivariate interaction of environmental, maturity, and nutrient parameters, using structural equation modeling (SEM). Results showed that the temperature of T2 compost with the microbial consortium increased more rapidly. The pH ranges of T1 (without the microbial consortium) and T2 were 8.75–9.15 and 8.42–9.27, respectively; the electrical conductivity (EC) ranges of T1 and T2 were 2.74–3.95 mS/cm and 2.81–3.85 mS/cm, respectively; the degradation rates of organic matter (OM) in T1 and T2 were 21.74% and 33.62%, respectively; and the total nitrogen (TN) ranges of T1 and T2 were 1.93–3.10% and 1.80–3.21%, respectively. By the end of composting, the germination indices (GI) of T1 and T2 were 20.57% and 64.24%, respectively. The total oxygen consumption after 4 days (AT₄) was 1.88 mg-O₂/g and 1.2 mg-O₂/g in T1 and T2, respectively. SEM of T1 showed that compost temperature and EC were important factors affecting compost maturity. These factors highly significantly affected OM, which in turn affected AT₄ of the biogas residue composting. SEM of T2 showed that compost temperature, pH, and EC affected OM, which in turn affected compost maturity. Temperature affected compost maturity by affecting AT₄ and GI. Principal component analysis (PCA) showed that the overall score of T2 was higher than that of T1, indicating that the addition of the microbial consortium was beneficial for industrial-scale composting of biogas residue produced by anaerobic digestion of food waste.

Anaerobic digestion of thermally hydrolyzed sludge was an important method for sludge treatment. But a large amount of rejected water (TRW) containing high ammonia was produced, which was difficult to treat. In this study, two-stage reactors were used for TRW treatment using partial nitrification-anammox technology. The results demonstrated that nitritation initiated rapidly. The NH₄⁺–N conversion load reached 1300 mg N/(L·day) and could be further improved. The consumption of NH₄⁺–N and the formation of NO₂⁻–N were linear with time. So, the right ratio of NH₄⁺–N/NO₂⁻–N can achieve by controlling time for anammox. Dissolved oxygen and sludge concentration had important effects on nitritation. Increasing dissolved oxygen or sludge concentration can shorten the reaction time. Nitrosomonas were the dominant ammonia-oxidizing bacteria, and nitrite-oxidizing bacteria were not detected. Aerobic treatment achieved more than 50% chemical oxygen demand removal in TRW, and this water was used in an anammox reactor. Accumulated flocculent sludge could inhibit anammox activity but could be removed by increasing the flow velocity. The anammox reactor load was above 600 mg N/(L·day); even under high conductivity (18.2 ms/cm) condition, the load reached 320 mg N/(L·day), and the total nitrogen removal rate was greater than 85% under stable condition. After approximately 190 days of operation, the abundance of anammox bacteria decreased from 29% to less than 10%, but the reactor operated stably. The results demonstrated two-stage reactors were suitable for ammonia removal in TRW using partial nitrification-anammox technology.

Swine manure treatment plants are important reservoirs of plasmid-harboring antibiotic resistance genes (ARGs) and physicochemical contaminants, but the changes in the abundances of plasmids and ARGs, and their interactions with the physicochemical properties of manure, are still unclear. Thus, in the present study, plasmidome and metagenome analyses were conducted for samples collected at different stages in the swine manure treatment process. The results indicated that anaerobic digestion and aerobic digestion were the most efficient stages for reducing the abundances of ARGs in swine manure. However, the plasmids associated with ARGs were not effectively removed in these stages. Through the whole treatment process, the IncL/M, IncQ1, IncHI2A, IncA/C, and IncN plasmid groups had strong correlations (r > 0.8, P < 0.01) with most ARG types, thereby indicating that these plasmids play important roles in the persistence of ARGs in this environment. Furthermore, the pH, total nitrogen, total phosphorus, and four heavy metals (Cu, Zn, As, and Fe) significantly affected the abundances of seven ARG subtypes (tetB(P), ant(6)-Ia, tet44, aph(3′′)-Ib, mefB, tet(L), and tet(39)). In particular, florfenicol had the most positive correlations with ARGs. Our results indicated that nutrients, heavy metals, and antibiotics all contributed to the presence and persistence of plasmid-harboring ARGs. This study provides insights into the fate of plasmids and ARGs, and related factors during the swine manure treatment process, thereby facilitating the development of a new treatment technique for removing ARGs and reducing the public health risk associated with livestock production.

Alkaline pretreatment (APT) is the promising disintegration pretreatment for the anaerobic digestion (AD) of sewage sludge (SS) to improve digestion efficiency and methane yields. In this study, the heavy metals (HMs) were observed to be leached from SS in the APT process, which could lower the HMs secondary pollution risk of the residual biosolids after AD in land application. The sequential chemical extraction (SCE) experiment was performed to determine the variation in HMs’ distribution prior to and after the APT. The alkaline extracts were characterized in order to elucidate the HMs’ release mechanism. The APT could cause significant release of Zn and Cu with a maximum release efficiency of 96.6% ± 4.6% and 62.7% ± 8.4% under the condition of 1.5 mol/L NaOH and 25 ℃, respectively. The release efficiency of Zn and Cu was reduced by 63.0% and 21.7%, respectively, due to the extra addition of 0.25 mol/L NaCl at a NaOH concentration of 1.25 mol/L in the APT process. The release of Zn and Cu may be attributed to a complex process including disruption of microbial cells in SS, solubilization of organic matters bounded with metals, and the chemical leaching reaction of minerals. This study demonstrates the possibility to remove the Zn and Cu from the SS in the APT process before the AD disposal.

The objective of this research was to evaluate anaerobic co-digestion of guinea pig manure (GP) with Andean agricultural residues such as amaranth (AM), quinoa (QU) and wheat (TR) in batch biodigesters under mesophilic conditions (37 ⁰C) for 40 days. As microbial inoculum, sewage treatment sludge was used in two inoculum/substrate ratios (ISR of 1 and 2). In terms of methane production, the best results occurred in treatments containing AM and QU as co-substrate and an ISR of 2. Thus, the highest methane production yield in the GP:AM biodigesters (25:75) and GP:QU (25:75) with 341.86 mlCH₄/g VS added and 341.05 mlCH₄/g VS added, respectively. On the other hand, the results showed that methane production with an ISR of 2 generated higher yields for guinea pig waste and the methane fraction of the biogas generated was in a range from 57 to 69%. Methane production kinetics from these raw materials was studied using five kinetic models: modified Gompertz, logistic equation, transfer, cone and Richards. The cone model adjusted best to the experimental values ​​with those observed with r² of 0.999 and RMSE of 1.16 mlCH₄/g VS added. Finally, the highest biodegradability (experimental yield/theoretical yield) was obtained in the GP-AM biodigesters (25:75) with 67.92%.